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The map label for this gene is iscS [H]

Identifier: 148642324

GI number: 148642324

Start: 246525

End: 247712

Strand: Reverse

Name: iscS [H]

Synonym: Msm_0264

Alternate gene names: 148642324

Gene position: 247712-246525 (Counterclockwise)

Preceding gene: 148642325

Following gene: 148642323

Centisome position: 13.37

GC content: 34.85

Gene sequence:

>1188_bases
ATGTATTTGGATAATTCAGCAACTACTGCAGTTAGTGAGGAAGTACTAAACGAAATGGAGCCTTATTTCAGGGAAGCCTA
TGGAAATCCGTCTACTTTGTACTCTGTTGGCCGCGAAGCTAAAAAGGGTTTAGATAAAGCAAGACAAAGAGTGGCTGATG
CAATTAATGCAGATCCTAAAGAAATAATATTCACTAGTGGAGGATCAGAATCTGATAATTTAGCTATTAAAGGTATTGCT
TTAAAATTAAAAGGTAAAGGAAACCACATTATCACTTCTGAAATTGAACATCCTGCAGTTAAAGAAACACTCCATTTCCT
TGAAACTTTAGATTTCAAAGTAACCTACCTGCCGGTTTATGAAAACGGTATTGTAAAAGTAGAGGATGTTAAAGATGCGA
TTACTCCAGAAACAATCTTAATTACCATTATGCATGGTAATAATGAAATTGGTACTCTTCAGCCAATTGCCGAGATTGGT
AAACTAGCCAGAGAACGTAAAATTCTTTTCCACACTGATGCAGTCCAGACATTCGGTAAAGTTAAAGTGGATGTTGAAGA
ATTGAACGTTGATTTATTATCCTTATCATCTCATAAAGTCCATGGTCCAAAAGGTGTTGGAGCATTATATATTAAAAAAG
GTGTTAGAATAACTCCTCTTATTCATGGTGGTGGCCAGGAAAGAGGAATCAGGTCAGGTACTGAAAATGTTCCAGGTATT
GTTGGATTCGGTAAAGCATGTGAAATAGCTAATAATAATCTGGAAGAAAATTATGCAAGATTAGTTAAAACAAGAGATGA
AATTATTGAAAAAGTATTGGATGCAGTTCCTGAATCTTATCTTAATGGTGATAGGAATGAAAGACTGCCTAATGTCATTA
ATTTCAGATTCTCATCTATTGAAGGTGAATCATTAATCCTCCTTTTAGATGCAAAAGGATATCAGGCATCAACAGGGTCA
GCCTGTTCATCTAAAACTTTAGAAGCATCTCCTGTATTAAAAGCTCTTAATCTTGATGATGTTGATGTACATGGTTCCTT
AAGAATATCTTTAGGTTCCCAAACTCACTTCGATGATGTTGACGGTTTAGTAGAAGCAATTAAAGAGTCAGTAGCTACTT
TAAGGAAAATGTCTCCTTTATGGAATTCTGATGAAAAATATGAAGAAATGATGGGTCAAAGTAAATAA

Upstream 100 bases:

>100_bases
ATCTATTGGTCTTGAAGATGCAGATGACTTGATTGATGATCTTTCACGTGCTTTAGATAAAATTTAAATTTTAATAAAAA
AATAAAAAGGAGATTATAAG

Downstream 100 bases:

>100_bases
ATTAAGGTGTTAAATATGTACAGTGAAAAAGTTATGGATCATTTTGCAAATCCAAGAAATTCAGGAGAAATGGAAAATCC
TGACGGAGTAGGAACAGTAG

Product: cysteine desulfurase, NifS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MYLDNSATTAVSEEVLNEMEPYFREAYGNPSTLYSVGREAKKGLDKARQRVADAINADPKEIIFTSGGSESDNLAIKGIA
LKLKGKGNHIITSEIEHPAVKETLHFLETLDFKVTYLPVYENGIVKVEDVKDAITPETILITIMHGNNEIGTLQPIAEIG
KLARERKILFHTDAVQTFGKVKVDVEELNVDLLSLSSHKVHGPKGVGALYIKKGVRITPLIHGGGQERGIRSGTENVPGI
VGFGKACEIANNNLEENYARLVKTRDEIIEKVLDAVPESYLNGDRNERLPNVINFRFSSIEGESLILLLDAKGYQASTGS
ACSSKTLEASPVLKALNLDDVDVHGSLRISLGSQTHFDDVDGLVEAIKESVATLRKMSPLWNSDEKYEEMMGQSK

Sequences:

>Translated_395_residues
MYLDNSATTAVSEEVLNEMEPYFREAYGNPSTLYSVGREAKKGLDKARQRVADAINADPKEIIFTSGGSESDNLAIKGIA
LKLKGKGNHIITSEIEHPAVKETLHFLETLDFKVTYLPVYENGIVKVEDVKDAITPETILITIMHGNNEIGTLQPIAEIG
KLARERKILFHTDAVQTFGKVKVDVEELNVDLLSLSSHKVHGPKGVGALYIKKGVRITPLIHGGGQERGIRSGTENVPGI
VGFGKACEIANNNLEENYARLVKTRDEIIEKVLDAVPESYLNGDRNERLPNVINFRFSSIEGESLILLLDAKGYQASTGS
ACSSKTLEASPVLKALNLDDVDVHGSLRISLGSQTHFDDVDGLVEAIKESVATLRKMSPLWNSDEKYEEMMGQSK
>Mature_395_residues
MYLDNSATTAVSEEVLNEMEPYFREAYGNPSTLYSVGREAKKGLDKARQRVADAINADPKEIIFTSGGSESDNLAIKGIA
LKLKGKGNHIITSEIEHPAVKETLHFLETLDFKVTYLPVYENGIVKVEDVKDAITPETILITIMHGNNEIGTLQPIAEIG
KLARERKILFHTDAVQTFGKVKVDVEELNVDLLSLSSHKVHGPKGVGALYIKKGVRITPLIHGGGQERGIRSGTENVPGI
VGFGKACEIANNNLEENYARLVKTRDEIIEKVLDAVPESYLNGDRNERLPNVINFRFSSIEGESLILLLDAKGYQASTGS
ACSSKTLEASPVLKALNLDDVDVHGSLRISLGSQTHFDDVDGLVEAIKESVATLRKMSPLWNSDEKYEEMMGQSK

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=384, Percent_Identity=45.3125, Blast_Score=360, Evalue=2e-99,
Organism=Homo sapiens, GI156713448, Length=411, Percent_Identity=37.956204379562, Blast_Score=246, Evalue=2e-65,
Organism=Escherichia coli, GI48994898, Length=386, Percent_Identity=46.3730569948187, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1789175, Length=398, Percent_Identity=26.1306532663317, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1787970, Length=387, Percent_Identity=26.0981912144703, Blast_Score=115, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI25143064, Length=385, Percent_Identity=44.1558441558442, Blast_Score=328, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI17533177, Length=322, Percent_Identity=35.4037267080745, Blast_Score=184, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6319831, Length=384, Percent_Identity=44.0104166666667, Blast_Score=337, Evalue=3e-93,
Organism=Drosophila melanogaster, GI20129463, Length=384, Percent_Identity=44.53125, Blast_Score=337, Evalue=7e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43288; Mature: 43288

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLDNSATTAVSEEVLNEMEPYFREAYGNPSTLYSVGREAKKGLDKARQRVADAINADPK
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EIIFTSGGSESDNLAIKGIALKLKGKGNHIITSEIEHPAVKETLHFLETLDFKVTYLPVY
HEEEECCCCCCCCEEEEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEE
ENGIVKVEDVKDAITPETILITIMHGNNEIGTLQPIAEIGKLARERKILFHTDAVQTFGK
CCCCEEEECCHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCEEEEEEHHHHHHCC
VKVDVEELNVDLLSLSSHKVHGPKGVGALYIKKGVRITPLIHGGGQERGIRSGTENVPGI
EEEEHHHHCEEEEECCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCE
VGFGKACEIANNNLEENYARLVKTRDEIIEKVLDAVPESYLNGDRNERLPNVINFRFSSI
ECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEECC
EGESLILLLDAKGYQASTGSACSSKTLEASPVLKALNLDDVDVHGSLRISLGSQTHFDDV
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEECCCEEEEEECCCCCCHHH
DGLVEAIKESVATLRKMSPLWNSDEKYEEMMGQSK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MYLDNSATTAVSEEVLNEMEPYFREAYGNPSTLYSVGREAKKGLDKARQRVADAINADPK
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EIIFTSGGSESDNLAIKGIALKLKGKGNHIITSEIEHPAVKETLHFLETLDFKVTYLPVY
HEEEECCCCCCCCEEEEEEEEEEECCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEE
ENGIVKVEDVKDAITPETILITIMHGNNEIGTLQPIAEIGKLARERKILFHTDAVQTFGK
CCCCEEEECCHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCEEEEEEHHHHHHCC
VKVDVEELNVDLLSLSSHKVHGPKGVGALYIKKGVRITPLIHGGGQERGIRSGTENVPGI
EEEEHHHHCEEEEECCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCE
VGFGKACEIANNNLEENYARLVKTRDEIIEKVLDAVPESYLNGDRNERLPNVINFRFSSI
ECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEECC
EGESLILLLDAKGYQASTGSACSSKTLEASPVLKALNLDDVDVHGSLRISLGSQTHFDDV
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEECCCEEEEEECCCCCCHHH
DGLVEAIKESVATLRKMSPLWNSDEKYEEMMGQSK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA