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The map label for this gene is amtB [C]

Identifier: 148642294

GI number: 148642294

Start: 221755

End: 222972

Strand: Reverse

Name: amtB [C]

Synonym: Msm_0234

Alternate gene names: 148642294

Gene position: 222972-221755 (Counterclockwise)

Preceding gene: 148642301

Following gene: 148642293

Centisome position: 12.03

GC content: 37.27

Gene sequence:

>1218_bases
ATGGATATATTTAGCGCAGGTGATACAGCTTGGGTTCTTGTTTGTAGTCTTTTAGTACTTTTAATGAGTATTCCGGCTGT
TGCATTCTTTTATGGTGGTTTAAGTAAACGTAAAAATGTTTTAAATACTATGTTTTTAACCTTTATTGCATTTTCAATTG
TAAGTGTTATTTGGGTTATTTTCGGTTATCAATTTGCCTTTGGAACTCCGTTACTGGGAGGATTTATAGGAAGCCCTTCT
AATTTCTTCTTAAGTGGAATCGGACTTGATGATTTAAGCGGAACTATTCCAACACTATTGTTTGTTATGTTCCAACTTGC
ATTTGCAGGTCTAACAGCAGCATTGATTTCTGGTGGTGTTGTTGGAAGAATGAAAACAAAAGCTTGGGTAATTTTTATCC
CGATTTGGGCTTGTCTTGTCTATATCCCTATTGCTCACTGGGTATGGGGTGGAGGATGGTTAATGAATATGGGAGCACTT
GATTTCGCAGGTGGTACTGTTGTACACATTAACTCAGGGGTTTCTGCATTAGCTATCGCACTTGTATTAGGTAAAAGAAA
AGACATTTCATTATTACCACATAACTTAGGATATGCAGTATTAGGTGCAGCACTTCTATGGTTCGGATGGATGGGATTCA
ACGGAGGATCCGGTTTAGGTGCAAATGGTCTTGCAGCAAATGCAATTATTGTTTCAAATGTTGCAGCTGCAGCAGCTTTA
ATTGTCTGGACTATATTAGATACAATTGTTGTTGGTAAACCAACTGTTCTTGGTGCAATCTCTGGAGGAATTGCTGGTTT
AGTAGCTATTACTCCTGCAGCAGGATTTGTTGATGTTCCTGGAGCATTAGTAATTGGTGCAGTAGCTCCTTTAGTATCTT
ACTTTGCAATTTATTACTTAAAAGCAAAACTCGGTTACGACGATGCATTAGACGTATTCGGTATACACGGTATGTCTGGT
ATATGGGGAGCAATCGCTACTGGTATCTTTGCAGCACCATATATTAATGAAGCAGCAGGATTATTATATGGAAACCCATA
TCAAGTTGTAATACAAATAATAAGTGTAGTAGCTACTATTGTTTATGCATTTGTAATAAGCTATGTATTAGCTAAAGTAC
TTGACAAAGCTATGGGCGGTATCAGAGTAGAAGATGAAGCTGAAGTTGTAGGATTAGATACAAAACTTCACGAAGAATCT
GCATACAATTTTAACTAG

Upstream 100 bases:

>100_bases
GATTCCGACTTCCGACTTCCGTTTAATCAAAATTCATAAAAATTTTAAAAAATAATTTTGATTTATTGTTATTAATTTAT
TTAAAAAGAGAAAAGATATA

Downstream 100 bases:

>100_bases
AAGGTTTAAAAATGAAAAGTATTGTTGCAATTATTAGACAGGAAAAGTATCAGGACGTTAAAAATGAACTTACAGCCGTG
GGCTGTCAAGGAATGAATGT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MDIFSAGDTAWVLVCSLLVLLMSIPAVAFFYGGLSKRKNVLNTMFLTFIAFSIVSVIWVIFGYQFAFGTPLLGGFIGSPS
NFFLSGIGLDDLSGTIPTLLFVMFQLAFAGLTAALISGGVVGRMKTKAWVIFIPIWACLVYIPIAHWVWGGGWLMNMGAL
DFAGGTVVHINSGVSALAIALVLGKRKDISLLPHNLGYAVLGAALLWFGWMGFNGGSGLGANGLAANAIIVSNVAAAAAL
IVWTILDTIVVGKPTVLGAISGGIAGLVAITPAAGFVDVPGALVIGAVAPLVSYFAIYYLKAKLGYDDALDVFGIHGMSG
IWGAIATGIFAAPYINEAAGLLYGNPYQVVIQIISVVATIVYAFVISYVLAKVLDKAMGGIRVEDEAEVVGLDTKLHEES
AYNFN

Sequences:

>Translated_405_residues
MDIFSAGDTAWVLVCSLLVLLMSIPAVAFFYGGLSKRKNVLNTMFLTFIAFSIVSVIWVIFGYQFAFGTPLLGGFIGSPS
NFFLSGIGLDDLSGTIPTLLFVMFQLAFAGLTAALISGGVVGRMKTKAWVIFIPIWACLVYIPIAHWVWGGGWLMNMGAL
DFAGGTVVHINSGVSALAIALVLGKRKDISLLPHNLGYAVLGAALLWFGWMGFNGGSGLGANGLAANAIIVSNVAAAAAL
IVWTILDTIVVGKPTVLGAISGGIAGLVAITPAAGFVDVPGALVIGAVAPLVSYFAIYYLKAKLGYDDALDVFGIHGMSG
IWGAIATGIFAAPYINEAAGLLYGNPYQVVIQIISVVATIVYAFVISYVLAKVLDKAMGGIRVEDEAEVVGLDTKLHEES
AYNFN
>Mature_405_residues
MDIFSAGDTAWVLVCSLLVLLMSIPAVAFFYGGLSKRKNVLNTMFLTFIAFSIVSVIWVIFGYQFAFGTPLLGGFIGSPS
NFFLSGIGLDDLSGTIPTLLFVMFQLAFAGLTAALISGGVVGRMKTKAWVIFIPIWACLVYIPIAHWVWGGGWLMNMGAL
DFAGGTVVHINSGVSALAIALVLGKRKDISLLPHNLGYAVLGAALLWFGWMGFNGGSGLGANGLAANAIIVSNVAAAAAL
IVWTILDTIVVGKPTVLGAISGGIAGLVAITPAAGFVDVPGALVIGAVAPLVSYFAIYYLKAKLGYDDALDVFGIHGMSG
IWGAIATGIFAAPYINEAAGLLYGNPYQVVIQIISVVATIVYAFVISYVLAKVLDKAMGGIRVEDEAEVVGLDTKLHEES
AYNFN

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=402, Percent_Identity=44.2786069651741, Blast_Score=302, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17550182, Length=438, Percent_Identity=26.9406392694064, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32564042, Length=425, Percent_Identity=26.8235294117647, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI133931100, Length=429, Percent_Identity=27.039627039627, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17550180, Length=428, Percent_Identity=25.9345794392523, Blast_Score=105, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6324187, Length=422, Percent_Identity=36.4928909952607, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6325396, Length=420, Percent_Identity=33.5714285714286, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6321559, Length=417, Percent_Identity=31.1750599520384, Blast_Score=190, Evalue=3e-49,
Organism=Drosophila melanogaster, GI161078314, Length=414, Percent_Identity=31.6425120772947, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 42361; Mature: 42361

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00457 NA_SOLUT_SYMP_2 ; PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIFSAGDTAWVLVCSLLVLLMSIPAVAFFYGGLSKRKNVLNTMFLTFIAFSIVSVIWVI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGYQFAFGTPLLGGFIGSPSNFFLSGIGLDDLSGTIPTLLFVMFQLAFAGLTAALISGGV
HHHHHHHCCCHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
VGRMKTKAWVIFIPIWACLVYIPIAHWVWGGGWLMNMGALDFAGGTVVHINSGVSALAIA
EEEECCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCEECCCCEEEEECCCHHHHHHH
LVLGKRKDISLLPHNLGYAVLGAALLWFGWMGFNGGSGLGANGLAANAIIVSNVAAAAAL
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IVWTILDTIVVGKPTVLGAISGGIAGLVAITPAAGFVDVPGALVIGAVAPLVSYFAIYYL
HHHHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KAKLGYDDALDVFGIHGMSGIWGAIATGIFAAPYINEAAGLLYGNPYQVVIQIISVVATI
HHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHCHHCCCCHHHHHHHHHHHHHHH
VYAFVISYVLAKVLDKAMGGIRVEDEAEVVGLDTKLHEESAYNFN
HHHHHHHHHHHHHHHHHHCCCEECCCCCEEECCHHHHHCCCCCCC
>Mature Secondary Structure
MDIFSAGDTAWVLVCSLLVLLMSIPAVAFFYGGLSKRKNVLNTMFLTFIAFSIVSVIWVI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGYQFAFGTPLLGGFIGSPSNFFLSGIGLDDLSGTIPTLLFVMFQLAFAGLTAALISGGV
HHHHHHHCCCHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
VGRMKTKAWVIFIPIWACLVYIPIAHWVWGGGWLMNMGALDFAGGTVVHINSGVSALAIA
EEEECCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCEECCCCEEEEECCCHHHHHHH
LVLGKRKDISLLPHNLGYAVLGAALLWFGWMGFNGGSGLGANGLAANAIIVSNVAAAAAL
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IVWTILDTIVVGKPTVLGAISGGIAGLVAITPAAGFVDVPGALVIGAVAPLVSYFAIYYL
HHHHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KAKLGYDDALDVFGIHGMSGIWGAIATGIFAAPYINEAAGLLYGNPYQVVIQIISVVATI
HHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHCHHCCCCHHHHHHHHHHHHHHH
VYAFVISYVLAKVLDKAMGGIRVEDEAEVVGLDTKLHEESAYNFN
HHHHHHHHHHHHHHHHHHCCCEECCCCCEEECCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]