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The map label for this gene is infB

Identifier: 148642262

GI number: 148642262

Start: 193349

End: 195139

Strand: Reverse

Name: infB

Synonym: Msm_0202

Alternate gene names: 148642262

Gene position: 195139-193349 (Counterclockwise)

Preceding gene: 148642263

Following gene: 148642261

Centisome position: 10.53

GC content: 33.67

Gene sequence:

>1791_bases
ATGAAAATCAGATCCCCAATTGTATCAGTTTTAGGACATGTAGACCACGGTAAGACCACTTTATTGGATTATATTAGAGG
AAGTACTATTGCAGCTAAGGAAGCTGGAGGTATTACTCAGCATATTGGTGCTACTGAGATTCCTAATGATACCATTGAAA
ATATCTGTGGAGATTTCATATCCAAATTAGCTATTAAAGATTTAATTCCTGGTTTGTTTTTCATTGATACTCCTGGACAT
GCCGCATTTACTAGTTTAAGAAAACGTGGTGGTGCATTAGCTGATTTGGCTGTATTGATTTTAGATGTTAATGACGGATT
TAAGCCACAGACTTATGAAGCATTAAACATTCTTAAAATGTATAAAACTCCATTTATTGTTGTAGCTAATAAAATCGATA
GGCTTTTTGGTTGGGAAGTTCATGAAGGTGCCTCTTTTAGGGAAACATTTTCCAATCAGGCTAAAAGTGTTCAGCAGGAT
TTGGACAATAAGATTTATGAAATTGTCGGTGAACTTCACAAGGAAGGATTCCAGTCTGAAAGATTTGACAGAGTAAGCAA
TTTTGCTTCCCAGATTAGTATTATTCCGATTAGTGCTAAAACTGGTGAAGGAGTTATAGAAGTTTTAGCTATGCTTTTAG
GGCTTGCTCAAGAATATTTAACTGAACAACTTGAAATCGATGAAAATGCTCCAGCTAAAGGTACAGTTTTAGAAATTAAG
GAAGAAACTGGTTTGGGAGTTACTCTTGATGCTATTATTTATGATGGTGTTTTAAGAACCAATGATGAAATAGCTTTAAT
GTTATCTTCAGAAGATGTATTGGTAACTAAAATTAGATCTATTTTAAGACCTCTTCCTCTTGAGGAAATGAGGGATTCTA
AAAAGAAATTTAGAAAATTAGATGAAGTTGTTGCAGCGGCAGGTATTAAAGTAGCTGCACCTCATTTAGATGATGTTGTT
TCAGGTTCTCCTCTTAGAGTTTTAAGTGAAGATACTGATGTTGAACAGGAGATTTTAAATGAAATTGACAACATTACTAT
TGATACAGAAGATGAAGGTATTTTAGTTAAAGCAGATACTATTGGTTCACTGGAAGCTGTTGTTAAGCTATTAAGGGAAA
TGGATATTCCAATTCGTGCAGCTGATATCGGTGATGTAAATCGTAGGGATATTATTAATTCTTCAATTGCTTATGATGAA
AATGAACTTCACGGAGCAATTATTGCTTTTAATGTGGATGTTCATCCAAATTCTGAAGAAGATCTTAATAATTCTGAAGT
CAAGCTCTTTTCAGGAGATGTAATTTATCAGATTCTTGAAGAGTATGAGGAATGGGTCAAGCAAAAGCAAGAAGATAAGA
AAAAGTCATTTTATGATGCTATAATTAAACCTGCAAAATTTGTTTCACTTCCAAAATTAGTGTTCCGTCAAAGTAAACCT
GCTATTATCGGTATTGAATCTTTAAGCGGAACTCTTAAACAAGGTCAACAATTGATTAATAAAGATGGGCATGTTGTTGG
TTCAATAGCTAGTATGGAAGATAAGGGTGAAACATTACCTGATATTTCCAGAGGTCAAAGGGTAGCTATGGCTATTAAAG
ATGCTATAGTTGGAAAAGACTTTGAAGAGGGAGACGAGTTATATGTTGATATTCCTGAAAAACATTACAAATACATTGAA
AGGGAATTTAAGGATAAATTGACTGAAGATGAATTTGAAACCTTATATGAGTTTTTGGAAATTAAACGTAAGCAGGATTC
TGATTGGGGAAGTTTTGGTCTTTTTGAATAA

Upstream 100 bases:

>100_bases
CAGAAAGAGAAATCAATTTATTCTTTAATGAAGATGAAATTTGCGATTACGAAATCGTTGATAACAAATTAATTTACGAA
TAATTATTATTTAAGATAAC

Downstream 100 bases:

>100_bases
TAATTAATATTTTTTATAAAACAATTTAAAGGAGGAATACCATGGCATTCAAAGTAGTTGTATCTCAAAAAGAGGTAACT
CATCAAATTGAAGTTGAAGA

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MKIRSPIVSVLGHVDHGKTTLLDYIRGSTIAAKEAGGITQHIGATEIPNDTIENICGDFISKLAIKDLIPGLFFIDTPGH
AAFTSLRKRGGALADLAVLILDVNDGFKPQTYEALNILKMYKTPFIVVANKIDRLFGWEVHEGASFRETFSNQAKSVQQD
LDNKIYEIVGELHKEGFQSERFDRVSNFASQISIIPISAKTGEGVIEVLAMLLGLAQEYLTEQLEIDENAPAKGTVLEIK
EETGLGVTLDAIIYDGVLRTNDEIALMLSSEDVLVTKIRSILRPLPLEEMRDSKKKFRKLDEVVAAAGIKVAAPHLDDVV
SGSPLRVLSEDTDVEQEILNEIDNITIDTEDEGILVKADTIGSLEAVVKLLREMDIPIRAADIGDVNRRDIINSSIAYDE
NELHGAIIAFNVDVHPNSEEDLNNSEVKLFSGDVIYQILEEYEEWVKQKQEDKKKSFYDAIIKPAKFVSLPKLVFRQSKP
AIIGIESLSGTLKQGQQLINKDGHVVGSIASMEDKGETLPDISRGQRVAMAIKDAIVGKDFEEGDELYVDIPEKHYKYIE
REFKDKLTEDEFETLYEFLEIKRKQDSDWGSFGLFE

Sequences:

>Translated_596_residues
MKIRSPIVSVLGHVDHGKTTLLDYIRGSTIAAKEAGGITQHIGATEIPNDTIENICGDFISKLAIKDLIPGLFFIDTPGH
AAFTSLRKRGGALADLAVLILDVNDGFKPQTYEALNILKMYKTPFIVVANKIDRLFGWEVHEGASFRETFSNQAKSVQQD
LDNKIYEIVGELHKEGFQSERFDRVSNFASQISIIPISAKTGEGVIEVLAMLLGLAQEYLTEQLEIDENAPAKGTVLEIK
EETGLGVTLDAIIYDGVLRTNDEIALMLSSEDVLVTKIRSILRPLPLEEMRDSKKKFRKLDEVVAAAGIKVAAPHLDDVV
SGSPLRVLSEDTDVEQEILNEIDNITIDTEDEGILVKADTIGSLEAVVKLLREMDIPIRAADIGDVNRRDIINSSIAYDE
NELHGAIIAFNVDVHPNSEEDLNNSEVKLFSGDVIYQILEEYEEWVKQKQEDKKKSFYDAIIKPAKFVSLPKLVFRQSKP
AIIGIESLSGTLKQGQQLINKDGHVVGSIASMEDKGETLPDISRGQRVAMAIKDAIVGKDFEEGDELYVDIPEKHYKYIE
REFKDKLTEDEFETLYEFLEIKRKQDSDWGSFGLFE
>Mature_596_residues
MKIRSPIVSVLGHVDHGKTTLLDYIRGSTIAAKEAGGITQHIGATEIPNDTIENICGDFISKLAIKDLIPGLFFIDTPGH
AAFTSLRKRGGALADLAVLILDVNDGFKPQTYEALNILKMYKTPFIVVANKIDRLFGWEVHEGASFRETFSNQAKSVQQD
LDNKIYEIVGELHKEGFQSERFDRVSNFASQISIIPISAKTGEGVIEVLAMLLGLAQEYLTEQLEIDENAPAKGTVLEIK
EETGLGVTLDAIIYDGVLRTNDEIALMLSSEDVLVTKIRSILRPLPLEEMRDSKKKFRKLDEVVAAAGIKVAAPHLDDVV
SGSPLRVLSEDTDVEQEILNEIDNITIDTEDEGILVKADTIGSLEAVVKLLREMDIPIRAADIGDVNRRDIINSSIAYDE
NELHGAIIAFNVDVHPNSEEDLNNSEVKLFSGDVIYQILEEYEEWVKQKQEDKKKSFYDAIIKPAKFVSLPKLVFRQSKP
AIIGIESLSGTLKQGQQLINKDGHVVGSIASMEDKGETLPDISRGQRVAMAIKDAIVGKDFEEGDELYVDIPEKHYKYIE
REFKDKLTEDEFETLYEFLEIKRKQDSDWGSFGLFE

Specific function: Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI84043963, Length=594, Percent_Identity=31.3131313131313, Blast_Score=326, Evalue=4e-89,
Organism=Homo sapiens, GI53729339, Length=256, Percent_Identity=33.203125, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI53729337, Length=256, Percent_Identity=33.203125, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI1789559, Length=462, Percent_Identity=27.2727272727273, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI212656558, Length=600, Percent_Identity=31.6666666666667, Blast_Score=322, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI71994658, Length=131, Percent_Identity=46.5648854961832, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6319282, Length=593, Percent_Identity=34.2327150084317, Blast_Score=337, Evalue=3e-93,
Organism=Saccharomyces cerevisiae, GI6324550, Length=584, Percent_Identity=26.1986301369863, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24656849, Length=589, Percent_Identity=31.918505942275, Blast_Score=321, Evalue=1e-87,
Organism=Drosophila melanogaster, GI28572034, Length=259, Percent_Identity=35.1351351351351, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI160714833, Length=90, Percent_Identity=52.2222222222222, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2P_METS3 (A5UJM9)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001272775.1
- ProteinModelPortal:   A5UJM9
- SMR:   A5UJM9
- STRING:   A5UJM9
- GeneID:   5215790
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_0202
- eggNOG:   arNOG04413
- HOGENOM:   HBG635083
- OMA:   SPLRIVP
- ProtClustDB:   PRK04004
- GO:   GO:0005622
- HAMAP:   MF_00100_A
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004544
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00491
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 66426; Mature: 66426

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSPIVSVLGHVDHGKTTLLDYIRGSTIAAKEAGGITQHIGATEIPNDTIENICGDFI
CCCHHHHHHHHHCCCCCHHHHHHHHCCCCEEHHHCCCCHHHCCCCCCCCHHHHHHHHHHH
SKLAIKDLIPGLFFIDTPGHAAFTSLRKRGGALADLAVLILDVNDGFKPQTYEALNILKM
HHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCHHEEEEEEEECCCCCCCCHHHHHHHHHH
YKTPFIVVANKIDRLFGWEVHEGASFRETFSNQAKSVQQDLDNKIYEIVGELHKEGFQSE
HCCCEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
RFDRVSNFASQISIIPISAKTGEGVIEVLAMLLGLAQEYLTEQLEIDENAPAKGTVLEIK
HHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
EETGLGVTLDAIIYDGVLRTNDEIALMLSSEDVLVTKIRSILRPLPLEEMRDSKKKFRKL
CCCCCCEEEHHHHHCCCEECCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
DEVVAAAGIKVAAPHLDDVVSGSPLRVLSEDTDVEQEILNEIDNITIDTEDEGILVKADT
HHHHHHCCCEEECCCHHHHHCCCCCEEECCCCCHHHHHHHHHCCCEEECCCCCEEEEECC
IGSLEAVVKLLREMDIPIRAADIGDVNRRDIINSSIAYDENELHGAIIAFNVDVHPNSEE
CCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHCCHHCCCCCCCCEEEEEEEEEECCCCCC
DLNNSEVKLFSGDVIYQILEEYEEWVKQKQEDKKKSFYDAIIKPAKFVSLPKLVFRQSKP
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
AIIGIESLSGTLKQGQQLINKDGHVVGSIASMEDKGETLPDISRGQRVAMAIKDAIVGKD
CEEEHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
FEEGDELYVDIPEKHYKYIEREFKDKLTEDEFETLYEFLEIKRKQDSDWGSFGLFE
CCCCCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKIRSPIVSVLGHVDHGKTTLLDYIRGSTIAAKEAGGITQHIGATEIPNDTIENICGDFI
CCCHHHHHHHHHCCCCCHHHHHHHHCCCCEEHHHCCCCHHHCCCCCCCCHHHHHHHHHHH
SKLAIKDLIPGLFFIDTPGHAAFTSLRKRGGALADLAVLILDVNDGFKPQTYEALNILKM
HHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCHHEEEEEEEECCCCCCCCHHHHHHHHHH
YKTPFIVVANKIDRLFGWEVHEGASFRETFSNQAKSVQQDLDNKIYEIVGELHKEGFQSE
HCCCEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
RFDRVSNFASQISIIPISAKTGEGVIEVLAMLLGLAQEYLTEQLEIDENAPAKGTVLEIK
HHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
EETGLGVTLDAIIYDGVLRTNDEIALMLSSEDVLVTKIRSILRPLPLEEMRDSKKKFRKL
CCCCCCEEEHHHHHCCCEECCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
DEVVAAAGIKVAAPHLDDVVSGSPLRVLSEDTDVEQEILNEIDNITIDTEDEGILVKADT
HHHHHHCCCEEECCCHHHHHCCCCCEEECCCCCHHHHHHHHHCCCEEECCCCCEEEEECC
IGSLEAVVKLLREMDIPIRAADIGDVNRRDIINSSIAYDENELHGAIIAFNVDVHPNSEE
CCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHCCHHCCCCCCCCEEEEEEEEEECCCCCC
DLNNSEVKLFSGDVIYQILEEYEEWVKQKQEDKKKSFYDAIIKPAKFVSLPKLVFRQSKP
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
AIIGIESLSGTLKQGQQLINKDGHVVGSIASMEDKGETLPDISRGQRVAMAIKDAIVGKD
CEEEHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
FEEGDELYVDIPEKHYKYIEREFKDKLTEDEFETLYEFLEIKRKQDSDWGSFGLFE
CCCCCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA