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The map label for this gene is frhB [H]

Identifier: 148642195

GI number: 148642195

Start: 129635

End: 130714

Strand: Reverse

Name: frhB [H]

Synonym: Msm_0135

Alternate gene names: 148642195

Gene position: 130714-129635 (Counterclockwise)

Preceding gene: 148642197

Following gene: 148642194

Centisome position: 7.05

GC content: 34.44

Gene sequence:

>1080_bases
ATGAGTAACAAGAAAACAGCTATGGTGGGAACACCTTGCCAAATATTAGCAGCTACAAAAATTAACAGATACGAAGAAAA
AACCGGCGGTTCTCCTATTGATGTTAAAATAGGTTTGTTTTGTATGGAAAACTTCTCATACCAATATCTTAAAAGATATT
TGAAAAGTCGCGATATTGAATTGTTTGAAGTTAAGGAATTTAGAATAGAAAAAGGACAATTTGTTGCATATCTGATAGAT
GGTAATGTATTTAAAATACCGATAGCTGAAACCGAACCTTTTACTCGTAAAAATTGCCATATTTGTACTGATTATACTTC
TGATGTTTCAGACATTTCTGTTGGATCTGTCGGTTCTCCGAAATATCACTCAACAGTTATTGTAAGAAGCCAAAAAGGTA
AGCAGATAATTGATGCATGTATAGCTGAAGGATATATTGAAGCTGAAGCGATTTCCAGAAAAGGTCAGGATTTGCTGGAA
AAAATAGCTAATCAAAAAATCAGCAAAAATACAAGAATCTATAAGAAAAGGGAAGCTATTGGAAGACCTGTTTTATCAAA
AAGACAAATATCCGAAGAAGAATTCTATGATGAATGCGGCAAATGTCAGTTTGACAACCTGCAAAATGATGTAATCAGTG
TAGGGTCATGTGTTTTATGCGGTGCCTGCGAATATGTCTGTCCGATTGATGCTGTTCAGATAAACAACAGAAAACCTGTA
TCAGTAAAAGAATGTGAAGAAGACTGTCATGCATGTTACTTTGCATGTCCTAGAACATTTATCAGTGATGCAATATATCC
TGAAGGAATTGATGAACAGCCATTAGGCGAATACCTTGAAATTTACTCTGTAAAAGCAGATTCCATTATGGGACAAGATG
GTGGTGTAGTATCAGCTATTTTAGTATATCTGCTTGAAAACAATATTGTTGATGAAGTTTCTGTTGTCGGTGAAGATAAG
GATGCGCCTTGGAGACCTGAATCTTATCTTACATCTAAAATCCAGGATGTTATTAAAGCTGCTGGAACTAAATACAGTAC
TACAACAATTGGATTTAAAGCATTAACAAATAAGAAGTAA

Upstream 100 bases:

>100_bases
GTTTTTAAAGGGATTTGTTTAGTTTGATAAAAAACTTTAAATGATATTACATCACATATATTATATTAATATGATTATGT
TAGCATAAGAGGTGAAAAAT

Downstream 100 bases:

>100_bases
ATCTTCTTGTTTTTACTTATTTTTTAAACTATTTATAATATAAAAAATACATTTATTTAATGGTGATATGATGGAATTAA
TGAAAGAGCTATCTTTAACT

Product: coenzyme F420-reducing hydrogenase, beta subunit

Products: NA

Alternate protein names: 8-hydroxy-5-deazaflavin-reducing hydrogenase subunit beta; FRH [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSNKKTAMVGTPCQILAATKINRYEEKTGGSPIDVKIGLFCMENFSYQYLKRYLKSRDIELFEVKEFRIEKGQFVAYLID
GNVFKIPIAETEPFTRKNCHICTDYTSDVSDISVGSVGSPKYHSTVIVRSQKGKQIIDACIAEGYIEAEAISRKGQDLLE
KIANQKISKNTRIYKKREAIGRPVLSKRQISEEEFYDECGKCQFDNLQNDVISVGSCVLCGACEYVCPIDAVQINNRKPV
SVKECEEDCHACYFACPRTFISDAIYPEGIDEQPLGEYLEIYSVKADSIMGQDGGVVSAILVYLLENNIVDEVSVVGEDK
DAPWRPESYLTSKIQDVIKAAGTKYSTTTIGFKALTNKK

Sequences:

>Translated_359_residues
MSNKKTAMVGTPCQILAATKINRYEEKTGGSPIDVKIGLFCMENFSYQYLKRYLKSRDIELFEVKEFRIEKGQFVAYLID
GNVFKIPIAETEPFTRKNCHICTDYTSDVSDISVGSVGSPKYHSTVIVRSQKGKQIIDACIAEGYIEAEAISRKGQDLLE
KIANQKISKNTRIYKKREAIGRPVLSKRQISEEEFYDECGKCQFDNLQNDVISVGSCVLCGACEYVCPIDAVQINNRKPV
SVKECEEDCHACYFACPRTFISDAIYPEGIDEQPLGEYLEIYSVKADSIMGQDGGVVSAILVYLLENNIVDEVSVVGEDK
DAPWRPESYLTSKIQDVIKAAGTKYSTTTIGFKALTNKK
>Mature_358_residues
SNKKTAMVGTPCQILAATKINRYEEKTGGSPIDVKIGLFCMENFSYQYLKRYLKSRDIELFEVKEFRIEKGQFVAYLIDG
NVFKIPIAETEPFTRKNCHICTDYTSDVSDISVGSVGSPKYHSTVIVRSQKGKQIIDACIAEGYIEAEAISRKGQDLLEK
IANQKISKNTRIYKKREAIGRPVLSKRQISEEEFYDECGKCQFDNLQNDVISVGSCVLCGACEYVCPIDAVQINNRKPVS
VKECEEDCHACYFACPRTFISDAIYPEGIDEQPLGEYLEIYSVKADSIMGQDGGVVSAILVYLLENNIVDEVSVVGEDKD
APWRPESYLTSKIQDVIKAAGTKYSTTTIGFKALTNKK

Specific function: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen [H]

COG id: COG1035

COG function: function code C; Coenzyme F420-reducing hydrogenase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the frhB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007516
- InterPro:   IPR017679
- InterPro:   IPR007525 [H]

Pfam domain/function: PF04432 FrhB_FdhB_C; PF04422 FrhB_FdhB_N [H]

EC number: =1.12.98.1 [H]

Molecular weight: Translated: 40156; Mature: 40025

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKKTAMVGTPCQILAATKINRYEEKTGGSPIDVKIGLFCMENFSYQYLKRYLKSRDIE
CCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCE
LFEVKEFRIEKGQFVAYLIDGNVFKIPIAETEPFTRKNCHICTDYTSDVSDISVGSVGSP
EEEHHHHEECCCCEEEEEECCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
KYHSTVIVRSQKGKQIIDACIAEGYIEAEAISRKGQDLLEKIANQKISKNTRIYKKREAI
CCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHH
GRPVLSKRQISEEEFYDECGKCQFDNLQNDVISVGSCVLCGACEYVCPIDAVQINNRKPV
CCCCHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHCCCHHHCCCHHHCCCCEEEECCCCCC
SVKECEEDCHACYFACPRTFISDAIYPEGIDEQPLGEYLEIYSVKADSIMGQDGGVVSAI
CHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHEECHHHHCCCCCCHHHHH
LVYLLENNIVDEVSVVGEDKDAPWRPESYLTSKIQDVIKAAGTKYSTTTIGFKALTNKK
HHHHHHCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCC
>Mature Secondary Structure 
SNKKTAMVGTPCQILAATKINRYEEKTGGSPIDVKIGLFCMENFSYQYLKRYLKSRDIE
CCCCCEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCE
LFEVKEFRIEKGQFVAYLIDGNVFKIPIAETEPFTRKNCHICTDYTSDVSDISVGSVGSP
EEEHHHHEECCCCEEEEEECCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
KYHSTVIVRSQKGKQIIDACIAEGYIEAEAISRKGQDLLEKIANQKISKNTRIYKKREAI
CCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHH
GRPVLSKRQISEEEFYDECGKCQFDNLQNDVISVGSCVLCGACEYVCPIDAVQINNRKPV
CCCCHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHCCCHHHCCCHHHCCCCEEEECCCCCC
SVKECEEDCHACYFACPRTFISDAIYPEGIDEQPLGEYLEIYSVKADSIMGQDGGVVSAI
CHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHEECHHHHCCCCCCHHHHH
LVYLLENNIVDEVSVVGEDKDAPWRPESYLTSKIQDVIKAAGTKYSTTTIGFKALTNKK
HHHHHHCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]