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The map label for this gene is frvX [C]

Identifier: 148642194

GI number: 148642194

Start: 128536

End: 129567

Strand: Reverse

Name: frvX [C]

Synonym: Msm_0134

Alternate gene names: 148642194

Gene position: 129567-128536 (Counterclockwise)

Preceding gene: 148642195

Following gene: 148642193

Centisome position: 6.99

GC content: 35.47

Gene sequence:

>1032_bases
ATGATGGAATTAATGAAAGAGCTATCTTTAACTCCAGGTGTTTCTGGATCTGAAGAAAAAATAGCAAACATTATTAAAAG
AGAATTAAAAGATGTCGCTGATTCTATTGAAGAAGACAGTATGGGTAATGTAATAGCTACTAAAAAAGGTGAGAAAAAAG
CACCTACTGTAATGTTAGCTTCCCATATGGATGAAATCGGATTGATGGTCAGATATATTGATGAAAAAGGATATGTCAAA
TTCTCAACAATTGGTGGAATTAATGACCAAATGTTAATGAATCAGTCTGTAATTATTCACAGCAGCATTGGAGAAGATGT
TCCGGGGGTTATTGGCTCAAAACCACCTCATGTTACAAAACCTGAAGAAAGAAACAAAGTTGTAAAAGCAGATGACATGT
TTATTGATATCGGTGCAAAAGACCAGGAAGATGCAGAAAAAATGATTCGTATTGGAGACAAAATAAGTTTCAGAGCTTGG
TTTGAAGAATATCCAAACGATTTGATTATGGGTAAAGCATTAGATAATCGTGTTGGCTGTTATGTAATGATGGAAGTTTT
AAAAAGAGTAAATACAAGAGCTACTGTTTACGGTGTAGGTACTGTACAGGAAGAAGTAGGTCTTAAAGGAGCAAAAACTT
CTGCATTTAAATTAAATCCAGACATGGCTATTGCATTAGATGTTACCTTATCTGGTGACCATCCGGGAATTAAACCTGAA
GAGGCACCAGTAGTAATGGGTAAAGGTCCGGCTATAATTTTAGCTGATGCAAGTGGACGCGGAATTCTTACTCAGCAGTC
AATTAAAGACTTGCTTATTAAAGCAGGTGATGAAAATGAAATTGATTACCAGCTTGAAGTAAGTGACGGCGGAACAACTG
ACGGTACAGCTATTCACTTAACAAGGGAAGGAATTCCAACTGGAGTTTTATCAGTTCCAACCAGATATATACACACTACT
GTAAGTGTTTGCAGTATGAAAGATGTTGAATCAACTATTCAATTAATTACAGAAGCTATAAATTCATTATAG

Upstream 100 bases:

>100_bases
ATTGGATTTAAAGCATTAACAAATAAGAAGTAAATCTTCTTGTTTTTACTTATTTTTTAAACTATTTATAATATAAAAAA
TACATTTATTTAATGGTGAT

Downstream 100 bases:

>100_bases
CTTTTTTTTAATTTTTTTTGGAGATTATGAAAAAATGTTTAGAACTATTGTCACACCAAAATTTGGAGATCTTGATGGTT
TAAGGCATATTAACAATAAT

Product: M42 glutamyl aminopeptidase/endo-glucanase

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MMELMKELSLTPGVSGSEEKIANIIKRELKDVADSIEEDSMGNVIATKKGEKKAPTVMLASHMDEIGLMVRYIDEKGYVK
FSTIGGINDQMLMNQSVIIHSSIGEDVPGVIGSKPPHVTKPEERNKVVKADDMFIDIGAKDQEDAEKMIRIGDKISFRAW
FEEYPNDLIMGKALDNRVGCYVMMEVLKRVNTRATVYGVGTVQEEVGLKGAKTSAFKLNPDMAIALDVTLSGDHPGIKPE
EAPVVMGKGPAIILADASGRGILTQQSIKDLLIKAGDENEIDYQLEVSDGGTTDGTAIHLTREGIPTGVLSVPTRYIHTT
VSVCSMKDVESTIQLITEAINSL

Sequences:

>Translated_343_residues
MMELMKELSLTPGVSGSEEKIANIIKRELKDVADSIEEDSMGNVIATKKGEKKAPTVMLASHMDEIGLMVRYIDEKGYVK
FSTIGGINDQMLMNQSVIIHSSIGEDVPGVIGSKPPHVTKPEERNKVVKADDMFIDIGAKDQEDAEKMIRIGDKISFRAW
FEEYPNDLIMGKALDNRVGCYVMMEVLKRVNTRATVYGVGTVQEEVGLKGAKTSAFKLNPDMAIALDVTLSGDHPGIKPE
EAPVVMGKGPAIILADASGRGILTQQSIKDLLIKAGDENEIDYQLEVSDGGTTDGTAIHLTREGIPTGVLSVPTRYIHTT
VSVCSMKDVESTIQLITEAINSL
>Mature_343_residues
MMELMKELSLTPGVSGSEEKIANIIKRELKDVADSIEEDSMGNVIATKKGEKKAPTVMLASHMDEIGLMVRYIDEKGYVK
FSTIGGINDQMLMNQSVIIHSSIGEDVPGVIGSKPPHVTKPEERNKVVKADDMFIDIGAKDQEDAEKMIRIGDKISFRAW
FEEYPNDLIMGKALDNRVGCYVMMEVLKRVNTRATVYGVGTVQEEVGLKGAKTSAFKLNPDMAIALDVTLSGDHPGIKPE
EAPVVMGKGPAIILADASGRGILTQQSIKDLLIKAGDENEIDYQLEVSDGGTTDGTAIHLTREGIPTGVLSVPTRYIHTT
VSVCSMKDVESTIQLITEAINSL

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=344, Percent_Identity=38.3720930232558, Blast_Score=246, Evalue=1e-66,
Organism=Escherichia coli, GI87082417, Length=334, Percent_Identity=33.8323353293413, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788727, Length=349, Percent_Identity=28.9398280802292, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 37236; Mature: 37236

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMELMKELSLTPGVSGSEEKIANIIKRELKDVADSIEEDSMGNVIATKKGEKKAPTVMLA
CHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEH
SHMDEIGLMVRYIDEKGYVKFSTIGGINDQMLMNQSVIIHSSIGEDVPGVIGSKPPHVTK
HHHHHHCEEEEEECCCCCEEEEEECCCCHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCC
PEERNKVVKADDMFIDIGAKDQEDAEKMIRIGDKISFRAWFEEYPNDLIMGKALDNRVGC
CHHHCCEEEECCEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCEEECCCCCCCCHH
YVMMEVLKRVNTRATVYGVGTVQEEVGLKGAKTSAFKLNPDMAIALDVTLSGDHPGIKPE
HHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCCEEEECCCEEEEEEEEECCCCCCCCCC
EAPVVMGKGPAIILADASGRGILTQQSIKDLLIKAGDENEIDYQLEVSDGGTTDGTAIHL
CCCEEECCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEE
TREGIPTGVLSVPTRYIHTTVSVCSMKDVESTIQLITEAINSL
ECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMELMKELSLTPGVSGSEEKIANIIKRELKDVADSIEEDSMGNVIATKKGEKKAPTVMLA
CHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEH
SHMDEIGLMVRYIDEKGYVKFSTIGGINDQMLMNQSVIIHSSIGEDVPGVIGSKPPHVTK
HHHHHHCEEEEEECCCCCEEEEEECCCCHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCC
PEERNKVVKADDMFIDIGAKDQEDAEKMIRIGDKISFRAWFEEYPNDLIMGKALDNRVGC
CHHHCCEEEECCEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCEEECCCCCCCCHH
YVMMEVLKRVNTRATVYGVGTVQEEVGLKGAKTSAFKLNPDMAIALDVTLSGDHPGIKPE
HHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCCEEEECCCEEEEEEEEECCCCCCCCCC
EAPVVMGKGPAIILADASGRGILTQQSIKDLLIKAGDENEIDYQLEVSDGGTTDGTAIHL
CCCEEECCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEE
TREGIPTGVLSVPTRYIHTTVSVCSMKDVESTIQLITEAINSL
ECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]