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The map label for this gene is spoIIIF [H]

Identifier: 148381476

GI number: 148381476

Start: 3778453

End: 3779988

Strand: Reverse

Name: spoIIIF [H]

Synonym: CBO3536

Alternate gene names: 148381476

Gene position: 3779988-3778453 (Counterclockwise)

Preceding gene: 148381477

Following gene: 148381475

Centisome position: 97.25

GC content: 29.17

Gene sequence:

>1536_bases
ATGAAAAAACAATCTTTAATAAAGGGAACTTTCATATTGGGAGTTGCAGGAATAATTGCTAAATTTTTAGGATTATTTTT
TAGATGGCCCTTACAAATGCTCATAGGAGATGAAGGTATTGGATACTATCAAATGTCCTATCCATTATATATGTTTTTTA
TAGCAGCTGCATCTGGAATACCTGTAGCAGTTTCTAAGTTAGTATCAGAAAGAAATGCAGTAAGAGATGAAGGTGGGATA
ATATCAGTTTTAAAAGAAGCTATGATATTTATGTTTATAATGGGCATGGGATTTACAATTATACTTTTATTATTTTCAAA
GGATATAATAAGATTTTTAAAATGGGATACAAGATCCTATTATTCTTTGATAGGAATATCTTTAGCACCTCTTTTTATTT
CTATAATGAGTGTGTTTAGGGGATTTTTTCAGGGAATGCAGAATATGAATTATACTGCCATATCACAGCTTATAGAGCAG
CTAGGGAGAGTAATATTCGGAGTTGGACTTGCCTATATATTATTACCAAAGGGGATAGAGTATTCAGCAGGAGGAGCAGC
TATAGGAGCAGCTGCAGGAGGCCTTTTAGGAGGAATATATCTTTTTTTAAAATATTTAGGTGTGAAAAAAGAATTTAGAG
TAAAAAAAGTAAAAAGAAATTTTAAAATAATGAATACTATACTTTATACGGCAATTCCTATATCCATAGGTTCTGCAGTA
GGAACTATCATGAGCTTAATAGATTCTGCATTAGTTCCACAGAAACTATTAGAAGCAGGGTTTACATACAAACAATCCAC
TATATTGTATGGGCAATTAACAGGAAAAGCATTTACTTTAGTTAATGTACCTTTAACATTATCTGTATCTTTGTGTGCAG
CATTGGTACCTATAATTGCAGAGGATTATATATTAAATAGAAAAATGGCAGTTTTGAAAAAAGTGGAATTAGCTATTAAA
ATTTCTATGGTTATAGCCGTACCTTCCTGTCTAGGACTTAATTTTATGGCTAAACCTATATTGAACTTAATATTTCCAGG
TCAAGAAGCCGGATATGAGATATTAAAGCATTTAGCACTAAGTATACCATTTATAGTATTGTGTCAAACTAGTACAGCTA
TATTACAAGGCATAGGAAGGTATATAAGGCCTATAATTAATTTATGTATAGGATGTATACTTAAAATAGTAATTACGTTG
ATTTTAGTCCCTATGAACAATATAAATATATATGGTGCAGTTATAGGCACAATAGCTGGTTATGTAATATCAGCCATATT
AAATATGATGTCTTTAAAAAGGAGTTTAAATATAAGTATCAATTATTATGAAATTATGATAAAACCTCTTATTGCATCTA
CAATAATGATAATTGCAGTTGTATTTATTTACTTCTATGCCTATAATTATACCATAAGTAGTAAAATAGCTTGTTTAATA
GCTGTATTTTTAGGAATGATAATCTACTTTATTATAATAGGATTAATTGGTATACTTGATTATAATTATATAAAGAGAAA
AATCATAAAAAGATAA

Upstream 100 bases:

>100_bases
CACAGGTTTTATTAATAAACTATAAAATTATAAAAATAAAAGTAATAATAACTCAGTTTTTAAAATACTAATAGGATAGT
AGTATTACGGAGGTATTATT

Downstream 100 bases:

>100_bases
GGGTGAATTATTACATGATAAACATTATAGGATTAGGTCCAGGGTCAAAAGAATCAATTACTTTAGGAACTATAGATAGT
TTAAAAACTGTTGATAAAGT

Product: stage V sporulation protein B

Products: NA

Alternate protein names: Stage III sporulation protein F [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MKKQSLIKGTFILGVAGIIAKFLGLFFRWPLQMLIGDEGIGYYQMSYPLYMFFIAAASGIPVAVSKLVSERNAVRDEGGI
ISVLKEAMIFMFIMGMGFTIILLLFSKDIIRFLKWDTRSYYSLIGISLAPLFISIMSVFRGFFQGMQNMNYTAISQLIEQ
LGRVIFGVGLAYILLPKGIEYSAGGAAIGAAAGGLLGGIYLFLKYLGVKKEFRVKKVKRNFKIMNTILYTAIPISIGSAV
GTIMSLIDSALVPQKLLEAGFTYKQSTILYGQLTGKAFTLVNVPLTLSVSLCAALVPIIAEDYILNRKMAVLKKVELAIK
ISMVIAVPSCLGLNFMAKPILNLIFPGQEAGYEILKHLALSIPFIVLCQTSTAILQGIGRYIRPIINLCIGCILKIVITL
ILVPMNNINIYGAVIGTIAGYVISAILNMMSLKRSLNISINYYEIMIKPLIASTIMIIAVVFIYFYAYNYTISSKIACLI
AVFLGMIIYFIIIGLIGILDYNYIKRKIIKR

Sequences:

>Translated_511_residues
MKKQSLIKGTFILGVAGIIAKFLGLFFRWPLQMLIGDEGIGYYQMSYPLYMFFIAAASGIPVAVSKLVSERNAVRDEGGI
ISVLKEAMIFMFIMGMGFTIILLLFSKDIIRFLKWDTRSYYSLIGISLAPLFISIMSVFRGFFQGMQNMNYTAISQLIEQ
LGRVIFGVGLAYILLPKGIEYSAGGAAIGAAAGGLLGGIYLFLKYLGVKKEFRVKKVKRNFKIMNTILYTAIPISIGSAV
GTIMSLIDSALVPQKLLEAGFTYKQSTILYGQLTGKAFTLVNVPLTLSVSLCAALVPIIAEDYILNRKMAVLKKVELAIK
ISMVIAVPSCLGLNFMAKPILNLIFPGQEAGYEILKHLALSIPFIVLCQTSTAILQGIGRYIRPIINLCIGCILKIVITL
ILVPMNNINIYGAVIGTIAGYVISAILNMMSLKRSLNISINYYEIMIKPLIASTIMIIAVVFIYFYAYNYTISSKIACLI
AVFLGMIIYFIIIGLIGILDYNYIKRKIIKR
>Mature_511_residues
MKKQSLIKGTFILGVAGIIAKFLGLFFRWPLQMLIGDEGIGYYQMSYPLYMFFIAAASGIPVAVSKLVSERNAVRDEGGI
ISVLKEAMIFMFIMGMGFTIILLLFSKDIIRFLKWDTRSYYSLIGISLAPLFISIMSVFRGFFQGMQNMNYTAISQLIEQ
LGRVIFGVGLAYILLPKGIEYSAGGAAIGAAAGGLLGGIYLFLKYLGVKKEFRVKKVKRNFKIMNTILYTAIPISIGSAV
GTIMSLIDSALVPQKLLEAGFTYKQSTILYGQLTGKAFTLVNVPLTLSVSLCAALVPIIAEDYILNRKMAVLKKVELAIK
ISMVIAVPSCLGLNFMAKPILNLIFPGQEAGYEILKHLALSIPFIVLCQTSTAILQGIGRYIRPIINLCIGCILKIVITL
ILVPMNNINIYGAVIGTIAGYVISAILNMMSLKRSLNISINYYEIMIKPLIASTIMIIAVVFIYFYAYNYTISSKIACLI
AVFLGMIIYFIIIGLIGILDYNYIKRKIIKR

Specific function: Involved, directly or indirectly, in spore cortex biosynthesis. Affects only indirectly the expression of late sporulation genes [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR002797
- InterPro:   IPR014249 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 56381; Mature: 56381

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1744050; 9384377 [H]