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The map label for this gene is glyS [H]

Identifier: 148381458

GI number: 148381458

Start: 3759022

End: 3760413

Strand: Reverse

Name: glyS [H]

Synonym: CBO3517

Alternate gene names: 148381458

Gene position: 3760413-3759022 (Counterclockwise)

Preceding gene: 148381459

Following gene: 148381457

Centisome position: 96.75

GC content: 31.68

Gene sequence:

>1392_bases
ATGGCTTTTAAAAAAACTATGGATAAAGTGGTTGCTTTAAGTAAAAATAGGGGATTTGTATTTCCGGGATCCGATATATA
TGGAGGATTGGCAAATTCATGGGATTATGGCCCACTAGGAGTAGAGCTAAAAAATAATGTGAAGAAAGCTTGGTGGAAAA
AATTTGTTCAAGAAAGTCCATATAATGTTGGAATAGATGCGGCTATTTTAATGAATAGCCAAGTTTGGGCAGCATCTGGG
CATGTAGGAGGATTTTCAGATCCGTTAATGGATTGTAAAGAATGTAAAGCAAGATTTAGAGCAGACAAACTAGTAGAAGA
GCACATGACAGAACAAGGTGTTGAAAAAGCTAGTGCAGATGGTTGGTCAAATGAAAAATTAAAACAGTATATAGATGATA
ATGGTATAGAATGTCCAAAATGTAAAGCTAAAAACTTTACAGATATAAGACAATTTAATTTAATGTTTAAGACATTTCAA
GGAGTAACAGAAGATGCAAAATCAGAAATATACTTAAGACCAGAAACAGCTCAAGGTATATTCGTTAATTTCAAAAATGT
TCAAAGAACATCTAGAAAGAAAGTACCATTTGGTATTGCTCAAATAGGAAAATCTTTTAGAAATGAAATAACTCCAGGAA
ACTTTACATTTAGAACTAGAGAATTTGAACAAATGGAATTAGAATTTTTCTGTAAGCCAGGAACAGATCTAGAATGGTTC
AATTATTGGAAAGATTATTGCTGGAATTTCTTAATAAATTTAGGTATAAACAAAGAAAATATAAGATTTAGAGATCATAG
TCAAGAAGAATTATCTCACTATAGTAATGCTACATCAGATATAGAATTTTTATTTCCATTTGGTTGGGGTGAGCTATGGG
GCGTTGCAGATAGAACAGATTTCGACTTGAAAAAGCATATGGAGCATTCAGGAGAAGATCTAAACTATTTAGATCCAGCA
ACTAAAGAAAGATATGTGCCATATTGTATAGAACCATCTTTAGGAGCGGATAGGGTTGTTTTAGCATTTTTAGTAGATGC
TTATGATGAAGAAGAACTAGAAGGTGGAGATGTGAGAAATGTATTACATTTACACCCAGCTTTGGCTCCATTTAAAGCAG
CAGTATTGCCACTTAGTAAAAAACTTTCTGATAAATCACAAGAAGTTTACAGTATGTTAAGCAAAAAGTTTAATATAGAT
TATGATGAGGCAGGCAGCATAGGAAAAAGATATAGAAGAGAAGATGAAATAGGAACTCCTTATTGTATAACAGTAGATTT
TGATACATTAGAAGATAATACGGTTACTATAAGAGACAGGGATACTATGAATCAAATAAGAGTCAATATAAATGAACTAG
AAAAATTTATAGAAGATAAATTAGAATTTTAA

Upstream 100 bases:

>100_bases
ATAGCCAAAAAGGGTGGAACCGCGATTAAGTCGTCCCTTTGTTGCTATAGCCTTTTTTATATTAAAAATTTTATGACAAA
ATATTAGGGAGGTACTTTTT

Downstream 100 bases:

>100_bases
AATAAAACCTATTAATAATAAAATTTAATAACATATAAATTTTATTATTAAATTAAAAAGCTATGAATTATAAAATATTA
TTTTATAATTCATAGCTTTA

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MAFKKTMDKVVALSKNRGFVFPGSDIYGGLANSWDYGPLGVELKNNVKKAWWKKFVQESPYNVGIDAAILMNSQVWAASG
HVGGFSDPLMDCKECKARFRADKLVEEHMTEQGVEKASADGWSNEKLKQYIDDNGIECPKCKAKNFTDIRQFNLMFKTFQ
GVTEDAKSEIYLRPETAQGIFVNFKNVQRTSRKKVPFGIAQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTDLEWF
NYWKDYCWNFLINLGINKENIRFRDHSQEELSHYSNATSDIEFLFPFGWGELWGVADRTDFDLKKHMEHSGEDLNYLDPA
TKERYVPYCIEPSLGADRVVLAFLVDAYDEEELEGGDVRNVLHLHPALAPFKAAVLPLSKKLSDKSQEVYSMLSKKFNID
YDEAGSIGKRYRREDEIGTPYCITVDFDTLEDNTVTIRDRDTMNQIRVNINELEKFIEDKLEF

Sequences:

>Translated_463_residues
MAFKKTMDKVVALSKNRGFVFPGSDIYGGLANSWDYGPLGVELKNNVKKAWWKKFVQESPYNVGIDAAILMNSQVWAASG
HVGGFSDPLMDCKECKARFRADKLVEEHMTEQGVEKASADGWSNEKLKQYIDDNGIECPKCKAKNFTDIRQFNLMFKTFQ
GVTEDAKSEIYLRPETAQGIFVNFKNVQRTSRKKVPFGIAQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTDLEWF
NYWKDYCWNFLINLGINKENIRFRDHSQEELSHYSNATSDIEFLFPFGWGELWGVADRTDFDLKKHMEHSGEDLNYLDPA
TKERYVPYCIEPSLGADRVVLAFLVDAYDEEELEGGDVRNVLHLHPALAPFKAAVLPLSKKLSDKSQEVYSMLSKKFNID
YDEAGSIGKRYRREDEIGTPYCITVDFDTLEDNTVTIRDRDTMNQIRVNINELEKFIEDKLEF
>Mature_462_residues
AFKKTMDKVVALSKNRGFVFPGSDIYGGLANSWDYGPLGVELKNNVKKAWWKKFVQESPYNVGIDAAILMNSQVWAASGH
VGGFSDPLMDCKECKARFRADKLVEEHMTEQGVEKASADGWSNEKLKQYIDDNGIECPKCKAKNFTDIRQFNLMFKTFQG
VTEDAKSEIYLRPETAQGIFVNFKNVQRTSRKKVPFGIAQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTDLEWFN
YWKDYCWNFLINLGINKENIRFRDHSQEELSHYSNATSDIEFLFPFGWGELWGVADRTDFDLKKHMEHSGEDLNYLDPAT
KERYVPYCIEPSLGADRVVLAFLVDAYDEEELEGGDVRNVLHLHPALAPFKAAVLPLSKKLSDKSQEVYSMLSKKFNIDY
DEAGSIGKRYRREDEIGTPYCITVDFDTLEDNTVTIRDRDTMNQIRVNINELEKFIEDKLEF

Specific function: Catalyzes the attachment of glycine to tRNA(Gly) [H]

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI116805340, Length=382, Percent_Identity=32.4607329842932, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI70887790, Length=311, Percent_Identity=23.7942122186495, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI32564541, Length=353, Percent_Identity=33.7110481586402, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17554996, Length=357, Percent_Identity=33.6134453781513, Blast_Score=170, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6325338, Length=365, Percent_Identity=30.958904109589, Blast_Score=176, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6319597, Length=404, Percent_Identity=28.4653465346535, Blast_Score=155, Evalue=9e-39,
Organism=Drosophila melanogaster, GI21357965, Length=363, Percent_Identity=32.7823691460055, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24664462, Length=363, Percent_Identity=32.7823691460055, Blast_Score=179, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022961
- InterPro:   IPR002315 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 53443; Mature: 53312

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50862 AA_TRNA_LIGASE_II ; PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFKKTMDKVVALSKNRGFVFPGSDIYGGLANSWDYGPLGVELKNNVKKAWWKKFVQESP
CCHHHHHHHHHHHCCCCCEECCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
YNVGIDAAILMNSQVWAASGHVGGFSDPLMDCKECKARFRADKLVEEHMTEQGVEKASAD
CCCCCCEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GWSNEKLKQYIDDNGIECPKCKAKNFTDIRQFNLMFKTFQGVTEDAKSEIYLRPETAQGI
CCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEE
FVNFKNVQRTSRKKVPFGIAQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTDLEWF
EEEEHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHEEEEEEECCCCCCHHH
NYWKDYCWNFLINLGINKENIRFRDHSQEELSHYSNATSDIEFLFPFGWGELWGVADRTD
HHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHCCCCCCEEEEEECCCHHHCCCCCCCC
FDLKKHMEHSGEDLNYLDPATKERYVPYCIEPSLGADRVVLAFLVDAYDEEELEGGDVRN
HHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCHHHCCCCCHHH
VLHLHPALAPFKAAVLPLSKKLSDKSQEVYSMLSKKFNIDYDEAGSIGKRYRREDEIGTP
HHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCCCCC
YCITVDFDTLEDNTVTIRDRDTMNQIRVNINELEKFIEDKLEF
EEEEEEECCCCCCEEEEECCCCHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AFKKTMDKVVALSKNRGFVFPGSDIYGGLANSWDYGPLGVELKNNVKKAWWKKFVQESP
CHHHHHHHHHHHCCCCCEECCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
YNVGIDAAILMNSQVWAASGHVGGFSDPLMDCKECKARFRADKLVEEHMTEQGVEKASAD
CCCCCCEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GWSNEKLKQYIDDNGIECPKCKAKNFTDIRQFNLMFKTFQGVTEDAKSEIYLRPETAQGI
CCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEE
FVNFKNVQRTSRKKVPFGIAQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTDLEWF
EEEEHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHEEEEEEECCCCCCHHH
NYWKDYCWNFLINLGINKENIRFRDHSQEELSHYSNATSDIEFLFPFGWGELWGVADRTD
HHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHCCCCCCEEEEEECCCHHHCCCCCCCC
FDLKKHMEHSGEDLNYLDPATKERYVPYCIEPSLGADRVVLAFLVDAYDEEELEGGDVRN
HHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCHHHCCCCCHHH
VLHLHPALAPFKAAVLPLSKKLSDKSQEVYSMLSKKFNIDYDEAGSIGKRYRREDEIGTP
HHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCCCCC
YCITVDFDTLEDNTVTIRDRDTMNQIRVNINELEKFIEDKLEF
EEEEEEECCCCCCEEEEECCCCHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA