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The map label for this gene is pbpA [H]

Identifier: 148381324

GI number: 148381324

Start: 3620993

End: 3622423

Strand: Reverse

Name: pbpA [H]

Synonym: CBO3383

Alternate gene names: 148381324

Gene position: 3622423-3620993 (Counterclockwise)

Preceding gene: 148381325

Following gene: 148381323

Centisome position: 93.2

GC content: 32.49

Gene sequence:

>1431_bases
ATGAAGGATATTTCAAATAATATAAAAAAAGTTTTATTAGTTTTTTTAATATGCTTTATGGGACTTATAACCTATATAAC
CTATTTTGAAATAGTAGTTGGGCCTAAAATAGTAGATAGTACTTATAATAGAAGGTTATGGGTAAAAAGAAATGAAGTGT
TAAGAGGAACTATATATGATAGAAACATGAAAGCACTAACTAAGAGTGAAAGAGTAAACAGTGAATTGCAAAAAAGAGAA
TATACTGGTAATTCTATGTTTGCTCATGTGTTGGGATATATTAATGTAAAGTATGGACTAACAGGGCTAGAAAAAAAGTA
TGATAAAGAATTAATGTCAACAGATATAAAAGATGATTTAACAACTTTCTTTAAAAATAAAGGTAAGTTGGAACAAAAGG
TAGGACATAATTTAAAAACAACATTAGACTATACAGTTCAAAAGGCTGCTTATGATGCTTTAGGGGACAATAAGGGATCA
GTAGTTGTATTAAATCCAAAAACAGGAGAAGTTTTAGGAATGGTATCTAAGCCTTCCTATGATCCTAATAAATTAGATGA
AATATGGGGAAGTATAAATAAAGATAAGAATATTCCCCTAATAAATAGAGCTACAGCAGGGTTGTATCCTCCAGGATCTA
CTTTTAAAATAGTAACTGCTTTAACTGCTTTAGAAAATATGTCTGGGGTAATGAATAGAACATTTCAAGACAATGGTAGT
TTAGATTTAGGGGGAGGATACTCCTTAAGCAACTATGGAGGAGCATCTTATGGAAGCCTTGATTTAAAAGGTGCCTTTGT
TCATTCAAGTAATGTAGTGTTTGGTAGTTTAGCCTTAGAACTAGGTAATTCAAGATTAAAGGGTACTACAGAAAAATTTT
ATTTTAATAAAGAAGTTCCTACAGATGGTATAGCTATAGAAAGTAGTAAGTTTCCAACTTTAAAGAGCTATGAAAAAGGA
AGTATAGCTCAAAGTGGAATAGGCCAAAGTTCTGTATTAGTAACTCCTATGGAGATGGCTTTGGTTGGAGCTACAGTTGC
TAATGACGGGGTTATGATGAAACCTTATTTAGTAAAAGAAGTTTTAAACAGCAAAGGAGAATTAATAAGAACTATTCCAC
CAGAATCTAATGGAGAAATAGTAAGTAAAAATAATGCCAGAATAGTTAAAGATTTTATGAAAGGTGTTGTAGAGGAAGGT
AGTGGAAGAAATGCTAGTATAGAAGGTATTCAAGTGGCCGGAAAGACTGGAACAGCAGATTATAGCGAATCTATTAAGGG
AATAGCAGCAGCACATTCTTGGTTTGTAGGTTTTGCACCTTATGATAATCCTCAAGTAGCTATAGCAGTAATTGTAGAAA
ACGGAGGTCAAGGTGGTATAGCTGCAGCTAGTATAGCTTCACAAGTAATGAGTACAGCGTTGAGCAAATAA

Upstream 100 bases:

>100_bases
ATAACATTACCTCTAGTAAGCCGTGGAGGAAGTTCCATGATGAGTATTTATATTTGTTTAGGTATATTACAAAAGATATC
AGAAGAGGGTAGATAAAATT

Downstream 100 bases:

>100_bases
TAAAATAGGGGTGAAGGATAAAGTCATATGAAAATTGCAGATATAACTAGTGAAAACAGTGGTGAAGTAGTAGAATTTAA
TGCCTTAGTTAATGATAAAA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBPA [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKDISNNIKKVLLVFLICFMGLITYITYFEIVVGPKIVDSTYNRRLWVKRNEVLRGTIYDRNMKALTKSERVNSELQKRE
YTGNSMFAHVLGYINVKYGLTGLEKKYDKELMSTDIKDDLTTFFKNKGKLEQKVGHNLKTTLDYTVQKAAYDALGDNKGS
VVVLNPKTGEVLGMVSKPSYDPNKLDEIWGSINKDKNIPLINRATAGLYPPGSTFKIVTALTALENMSGVMNRTFQDNGS
LDLGGGYSLSNYGGASYGSLDLKGAFVHSSNVVFGSLALELGNSRLKGTTEKFYFNKEVPTDGIAIESSKFPTLKSYEKG
SIAQSGIGQSSVLVTPMEMALVGATVANDGVMMKPYLVKEVLNSKGELIRTIPPESNGEIVSKNNARIVKDFMKGVVEEG
SGRNASIEGIQVAGKTGTADYSESIKGIAAAHSWFVGFAPYDNPQVAIAVIVENGGQGGIAAASIASQVMSTALSK

Sequences:

>Translated_476_residues
MKDISNNIKKVLLVFLICFMGLITYITYFEIVVGPKIVDSTYNRRLWVKRNEVLRGTIYDRNMKALTKSERVNSELQKRE
YTGNSMFAHVLGYINVKYGLTGLEKKYDKELMSTDIKDDLTTFFKNKGKLEQKVGHNLKTTLDYTVQKAAYDALGDNKGS
VVVLNPKTGEVLGMVSKPSYDPNKLDEIWGSINKDKNIPLINRATAGLYPPGSTFKIVTALTALENMSGVMNRTFQDNGS
LDLGGGYSLSNYGGASYGSLDLKGAFVHSSNVVFGSLALELGNSRLKGTTEKFYFNKEVPTDGIAIESSKFPTLKSYEKG
SIAQSGIGQSSVLVTPMEMALVGATVANDGVMMKPYLVKEVLNSKGELIRTIPPESNGEIVSKNNARIVKDFMKGVVEEG
SGRNASIEGIQVAGKTGTADYSESIKGIAAAHSWFVGFAPYDNPQVAIAVIVENGGQGGIAAASIASQVMSTALSK
>Mature_476_residues
MKDISNNIKKVLLVFLICFMGLITYITYFEIVVGPKIVDSTYNRRLWVKRNEVLRGTIYDRNMKALTKSERVNSELQKRE
YTGNSMFAHVLGYINVKYGLTGLEKKYDKELMSTDIKDDLTTFFKNKGKLEQKVGHNLKTTLDYTVQKAAYDALGDNKGS
VVVLNPKTGEVLGMVSKPSYDPNKLDEIWGSINKDKNIPLINRATAGLYPPGSTFKIVTALTALENMSGVMNRTFQDNGS
LDLGGGYSLSNYGGASYGSLDLKGAFVHSSNVVFGSLALELGNSRLKGTTEKFYFNKEVPTDGIAIESSKFPTLKSYEKG
SIAQSGIGQSSVLVTPMEMALVGATVANDGVMMKPYLVKEVLNSKGELIRTIPPESNGEIVSKNNARIVKDFMKGVVEEG
SGRNASIEGIQVAGKTGTADYSESIKGIAAAHSWFVGFAPYDNPQVAIAVIVENGGQGGIAAASIASQVMSTALSK

Specific function: Cell wall formation. Plays an important role in cell division and cell shape maintenance by cross-linking adjacent peptidoglycan chains through transpeptidation [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Probable). Note=Localized to the division site (septum) along with newly synthesized peptidoglycan, between segregated nucleoids (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=452, Percent_Identity=28.0973451327434, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1786272, Length=411, Percent_Identity=27.4939172749392, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 51575; Mature: 51575

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00337 BETA_LACTAMASE_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDISNNIKKVLLVFLICFMGLITYITYFEIVVGPKIVDSTYNRRLWVKRNEVLRGTIYD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEECCCEEEEEEEC
RNMKALTKSERVNSELQKREYTGNSMFAHVLGYINVKYGLTGLEKKYDKELMSTDIKDDL
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCHHHHH
TTFFKNKGKLEQKVGHNLKTTLDYTVQKAAYDALGDNKGSVVVLNPKTGEVLGMVSKPSY
HHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEECCCCCC
DPNKLDEIWGSINKDKNIPLINRATAGLYPPGSTFKIVTALTALENMSGVMNRTFQDNGS
CHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
LDLGGGYSLSNYGGASYGSLDLKGAFVHSSNVVFGSLALELGNSRLKGTTEKFYFNKEVP
EECCCCCEECCCCCCCCCCEEEEEEEEECCCEEEEEHHHHHCCCCCCCCCHHEEECCCCC
TDGIAIESSKFPTLKSYEKGSIAQSGIGQSSVLVTPMEMALVGATVANDGVMMKPYLVKE
CCCEEEECCCCCCCCCCCCCCCHHCCCCCCCEEECHHHHHHHHHEECCCCEEECHHHHHH
VLNSKGELIRTIPPESNGEIVSKNNARIVKDFMKGVVEEGSGRNASIEGIQVAGKTGTAD
HHCCCCCEEEECCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
YSESIKGIAAAHSWFVGFAPYDNPQVAIAVIVENGGQGGIAAASIASQVMSTALSK
HHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDISNNIKKVLLVFLICFMGLITYITYFEIVVGPKIVDSTYNRRLWVKRNEVLRGTIYD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEECCCEEEEEEEC
RNMKALTKSERVNSELQKREYTGNSMFAHVLGYINVKYGLTGLEKKYDKELMSTDIKDDL
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCHHHHH
TTFFKNKGKLEQKVGHNLKTTLDYTVQKAAYDALGDNKGSVVVLNPKTGEVLGMVSKPSY
HHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEECCCCCC
DPNKLDEIWGSINKDKNIPLINRATAGLYPPGSTFKIVTALTALENMSGVMNRTFQDNGS
CHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
LDLGGGYSLSNYGGASYGSLDLKGAFVHSSNVVFGSLALELGNSRLKGTTEKFYFNKEVP
EECCCCCEECCCCCCCCCCEEEEEEEEECCCEEEEEHHHHHCCCCCCCCCHHEEECCCCC
TDGIAIESSKFPTLKSYEKGSIAQSGIGQSSVLVTPMEMALVGATVANDGVMMKPYLVKE
CCCEEEECCCCCCCCCCCCCCCHHCCCCCCCEEECHHHHHHHHHEECCCCEEECHHHHHH
VLNSKGELIRTIPPESNGEIVSKNNARIVKDFMKGVVEEGSGRNASIEGIQVAGKTGTAD
HHCCCCCEEEECCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
YSESIKGIAAAHSWFVGFAPYDNPQVAIAVIVENGGQGGIAAASIASQVMSTALSK
HHHHHHHHHHHHHHEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA