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The map label for this gene is ygcA [C]

Identifier: 148381310

GI number: 148381310

Start: 3602179

End: 3603564

Strand: Reverse

Name: ygcA [C]

Synonym: CBO3369

Alternate gene names: 148381310

Gene position: 3603564-3602179 (Counterclockwise)

Preceding gene: 148381311

Following gene: 148381309

Centisome position: 92.71

GC content: 28.64

Gene sequence:

>1386_bases
ATGAAAAAAAATATACCTGTAGAAAAAAATAAAGAATACAATTTAGACATAATAGGCACTGGATTTGAAGGAGAAGGAGT
AAGTAAAATTGATGATTTTACAGTATTTATTCCAGGAGCCATAAAAGGAGAAAATGCATTAACAAGAATAGTTAAAGTAA
ATAAAAACTTCGCCTTCGGAAAGATTATAAAGTTAAACAAAACATCAGAAAATAGAGTAGAATCTGTATGTCCTATATAT
AAACAGTGTGGAGGATGTCAAGTTCAACACTATAGTTATAAGGCTCAATTAGAGTTTAAAAAGCAGAGGGTTATAGATTC
ACTAGAAAGAATAGGTAAACTAGATATAGATAAAATAAAAATAGAAGATACTATTGGCATGGAAAACCCGTATAGATATA
GAAACAAAATTCAAATGCCTGTAAGATTTGAAAAAGGTGAAATAAAAATAGGATTTTATAAATCAAGAACTCATGAAGTA
GCAGATGTAAAAGAATGTTTTATACAAGATGAGCAAGCAGATAAAATTATACAAATAATAAGAAATTGGATGGTAAAACA
TAATATATCTGCCTACAATGAAGAAACAGGAAAAGGGTTTATAAGGCATATAATGGTAAGGAAAGCTTTTAAAAATAAAG
AACTTATGTTAGTTATTGTAACTAATAAAGAAGAGGAAATACCTTACAAAGAAGGATTAATAAATGAAGTAATAAAAGAA
TTTTCAAATATAAAAAGTATAATACAAAATATAAATACTAAAAAAACCAATGTGATTTTAGGAGAAAAGTGTATTACATT
ATGGGGAAAAGATACCATAACTGACTATATAGATAAGTTTAAATTTAATATATCTCCATTATCTTTTTTTCAGGTAAATC
CAATACAAACAGAGGTATTGTATAAAAAAGCCTTAGAATTTGCAAATCTAAAAGGAGAAGAAATAGTATTTGATGCTTAC
TGTGGCACAGGAACTATATCCTTATTCTTATCACAGAAAGCTAAAAAAGTATACGGAGTAGAAATAATAAAGGAAGCTAT
AGACAACGCAAAAGATAATGCAAAAGAAAATAAGATAGATAATGCAGAATTTTTTGTAGGAAAATCAGAAGAAGTTATAC
CAGATTTAATAGATAAAGGTATAAAAGCAGATGTGGTAGTTGTAGATCCACCAAGAAAAGGCTGCGAAAAAACCTTACTA
GAAGCTATAGCAGCTACAAAACCTAAAAGAATAGTATATGTATCCTGTGATCCAGGAACTTTGGCTAGAGATTTAGGTAT
ATTAAATGGATTAGGTTATGAAACTAGAGAAGTACAACCAGTAGATATGTTCCCACAGACAAGTCACGTGGAGACTTGTG
TGTTGCTACAAAGAAAAACTATGTAG

Upstream 100 bases:

>100_bases
AATTTATATATTGGAAAGAGCCTAAGGATAGATAGTCTAATTGTTACTATTTATCCTGTGTTTTTGTTATGATATAAAAT
TACATAAATGGAGAGAGAGT

Downstream 100 bases:

>100_bases
AAATCCTTAATTTTAGGTACTTCGTCAACTATTTTATTTTTACAAATGATGGGGATAAAATCCGAAATAAACAGTTAAAA
AAATATATTAACGTTTCTGT

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKKNIPVEKNKEYNLDIIGTGFEGEGVSKIDDFTVFIPGAIKGENALTRIVKVNKNFAFGKIIKLNKTSENRVESVCPIY
KQCGGCQVQHYSYKAQLEFKKQRVIDSLERIGKLDIDKIKIEDTIGMENPYRYRNKIQMPVRFEKGEIKIGFYKSRTHEV
ADVKECFIQDEQADKIIQIIRNWMVKHNISAYNEETGKGFIRHIMVRKAFKNKELMLVIVTNKEEEIPYKEGLINEVIKE
FSNIKSIIQNINTKKTNVILGEKCITLWGKDTITDYIDKFKFNISPLSFFQVNPIQTEVLYKKALEFANLKGEEIVFDAY
CGTGTISLFLSQKAKKVYGVEIIKEAIDNAKDNAKENKIDNAEFFVGKSEEVIPDLIDKGIKADVVVVDPPRKGCEKTLL
EAIAATKPKRIVYVSCDPGTLARDLGILNGLGYETREVQPVDMFPQTSHVETCVLLQRKTM

Sequences:

>Translated_461_residues
MKKNIPVEKNKEYNLDIIGTGFEGEGVSKIDDFTVFIPGAIKGENALTRIVKVNKNFAFGKIIKLNKTSENRVESVCPIY
KQCGGCQVQHYSYKAQLEFKKQRVIDSLERIGKLDIDKIKIEDTIGMENPYRYRNKIQMPVRFEKGEIKIGFYKSRTHEV
ADVKECFIQDEQADKIIQIIRNWMVKHNISAYNEETGKGFIRHIMVRKAFKNKELMLVIVTNKEEEIPYKEGLINEVIKE
FSNIKSIIQNINTKKTNVILGEKCITLWGKDTITDYIDKFKFNISPLSFFQVNPIQTEVLYKKALEFANLKGEEIVFDAY
CGTGTISLFLSQKAKKVYGVEIIKEAIDNAKDNAKENKIDNAEFFVGKSEEVIPDLIDKGIKADVVVVDPPRKGCEKTLL
EAIAATKPKRIVYVSCDPGTLARDLGILNGLGYETREVQPVDMFPQTSHVETCVLLQRKTM
>Mature_461_residues
MKKNIPVEKNKEYNLDIIGTGFEGEGVSKIDDFTVFIPGAIKGENALTRIVKVNKNFAFGKIIKLNKTSENRVESVCPIY
KQCGGCQVQHYSYKAQLEFKKQRVIDSLERIGKLDIDKIKIEDTIGMENPYRYRNKIQMPVRFEKGEIKIGFYKSRTHEV
ADVKECFIQDEQADKIIQIIRNWMVKHNISAYNEETGKGFIRHIMVRKAFKNKELMLVIVTNKEEEIPYKEGLINEVIKE
FSNIKSIIQNINTKKTNVILGEKCITLWGKDTITDYIDKFKFNISPLSFFQVNPIQTEVLYKKALEFANLKGEEIVFDAY
CGTGTISLFLSQKAKKVYGVEIIKEAIDNAKDNAKENKIDNAEFFVGKSEEVIPDLIDKGIKADVVVVDPPRKGCEKTLL
EAIAATKPKRIVYVSCDPGTLARDLGILNGLGYETREVQPVDMFPQTSHVETCVLLQRKTM

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=350, Percent_Identity=28.5714285714286, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI51173878, Length=350, Percent_Identity=28.5714285714286, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI269784680, Length=206, Percent_Identity=32.0388349514563, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI269784676, Length=206, Percent_Identity=32.0388349514563, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI20149688, Length=206, Percent_Identity=32.0388349514563, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI269784678, Length=206, Percent_Identity=32.0388349514563, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1789148, Length=445, Percent_Identity=27.1910112359551, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1787083, Length=325, Percent_Identity=25.5384615384615, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790403, Length=185, Percent_Identity=30.8108108108108, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71997438, Length=166, Percent_Identity=36.7469879518072, Blast_Score=92, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6322909, Length=450, Percent_Identity=25.3333333333333, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24666579, Length=437, Percent_Identity=29.5194508009153, Blast_Score=164, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 52578; Mature: 52578

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNIPVEKNKEYNLDIIGTGFEGEGVSKIDDFTVFIPGAIKGENALTRIVKVNKNFAFG
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHEEEEEECCCCCEE
KIIKLNKTSENRVESVCPIYKQCGGCQVQHYSYKAQLEFKKQRVIDSLERIGKLDIDKIK
EEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCEEE
IEDTIGMENPYRYRNKIQMPVRFEKGEIKIGFYKSRTHEVADVKECFIQDEQADKIIQII
EECCCCCCCCHHHHCEECCCEEEECCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHH
RNWMVKHNISAYNEETGKGFIRHIMVRKAFKNKELMLVIVTNKEEEIPYKEGLINEVIKE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
FSNIKSIIQNINTKKTNVILGEKCITLWGKDTITDYIDKFKFNISPLSFFQVNPIQTEVL
HHHHHHHHHCCCCCCCEEEECCCEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHH
YKKALEFANLKGEEIVFDAYCGTGTISLFLSQKAKKVYGVEIIKEAIDNAKDNAKENKID
HHHHHHHHCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC
NAEFFVGKSEEVIPDLIDKGIKADVVVVDPPRKGCEKTLLEAIAATKPKRIVYVSCDPGT
CCEEEECCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCH
LARDLGILNGLGYETREVQPVDMFPQTSHVETCVLLQRKTM
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEEEECCCC
>Mature Secondary Structure
MKKNIPVEKNKEYNLDIIGTGFEGEGVSKIDDFTVFIPGAIKGENALTRIVKVNKNFAFG
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHEEEEEECCCCCEE
KIIKLNKTSENRVESVCPIYKQCGGCQVQHYSYKAQLEFKKQRVIDSLERIGKLDIDKIK
EEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCEEE
IEDTIGMENPYRYRNKIQMPVRFEKGEIKIGFYKSRTHEVADVKECFIQDEQADKIIQII
EECCCCCCCCHHHHCEECCCEEEECCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHH
RNWMVKHNISAYNEETGKGFIRHIMVRKAFKNKELMLVIVTNKEEEIPYKEGLINEVIKE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
FSNIKSIIQNINTKKTNVILGEKCITLWGKDTITDYIDKFKFNISPLSFFQVNPIQTEVL
HHHHHHHHHCCCCCCCEEEECCCEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHH
YKKALEFANLKGEEIVFDAYCGTGTISLFLSQKAKKVYGVEIIKEAIDNAKDNAKENKID
HHHHHHHHCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC
NAEFFVGKSEEVIPDLIDKGIKADVVVVDPPRKGCEKTLLEAIAATKPKRIVYVSCDPGT
CCEEEECCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCH
LARDLGILNGLGYETREVQPVDMFPQTSHVETCVLLQRKTM
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]