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The map label for this gene is vanS [H]

Identifier: 148381289

GI number: 148381289

Start: 3579154

End: 3580539

Strand: Reverse

Name: vanS [H]

Synonym: CBO3349

Alternate gene names: 148381289

Gene position: 3580539-3579154 (Counterclockwise)

Preceding gene: 148381290

Following gene: 148381288

Centisome position: 92.12

GC content: 25.11

Gene sequence:

>1386_bases
TTGAAAAGGAAGCTTAGAATTGCATTAACAACAGAACTGATGCTACTTTTTGTTATATCTTTAATAATTACAATTTTTGG
AATGATGATGGTTTTAGGGAGTCTAAATATTTCATCGTATAATATTAATAAATTTTTATTCCCAGAACATTTTAAGGATA
AGGTTATAATAGAAGAATATGAGAAAACTGTTGGAAGGCGTGTAGATAGGGTCATAAAAGGAAATATAAATCAGCTTAAA
GTACCACATAAAATGGATGAAAAGCTTAAAAAAGAGTTTCCATATAATTTTAGAGGGAAAACTTTAGTTTATATTATTAA
TTCTAATGGGGAAATACTAAGTTCTAATAATGAAGAAAATATGTATACTATACGTAAATCTACAATAAAAGAAGGACTAG
AGTTAAAAAGATATGATGATAATATTATTATAAATATTAGAGAAACAAAAAAAATAAGTGATAACTTATATATAGTAATA
AATAATGATTCTGTTATAGATGGAGATTTTATTATAATGTGTACGGGTTTATTTGCATTTATGATTATTTTTGCCATACT
TGCAAAGGGAAGGTTAAAGTATATCATATCTATTAAAAAAGGAGTAAACAAGCTATATTCATCAGATTTTGAAGATAAAA
TACCATTAAAATATAATAATGAGCTTACATCTCTGGCAATTGCATTAAATGATATGGGAGGAAAAATAAAAGAAAATAAG
GAAAATGAAAAAGAATTTTTATTAAATATATCCCATGACTTAAGGACTCCATTAACCTCTATATTAGGGTTTCTAAATCT
TTTAAAGCAGAAGAAATATGATATAGAAGAGGAGAGGGAAGGTTATATAAATACTATTGAAGAACAAAGTTTATATTTGA
AGACTCTTATAGATGAATTTTTTCAATTTTCAACGCTAAAATGGAAAAATGTTAAACTAAATAAAGAAAATATAAATCTT
CAAGAGATTTTACGCCAAATAAGTGATGGATTTTATCCTCAACTAAAAGAAAATAAAATTAGTGTGTCTATGAATTTTCC
TGAAAAATCTTTATATAAAGAGGTTGATGTTGATAAGTTTATGAGAGTATTAGAAAATGTAATATCTAATGCTATAAAAT
ATAGCCATAAAGGAACAGAAATTAGTTTAAATCTATATGAAAATAAAGATAAAATAGTAATGGAATTTTGGAATACTCCT
ATAGAAGCTCTAAAGGAAGATGAGTTAGATTTTTTATTTAAAAGATTTTATAAAAAAGATTTATCAAGAGGTAATAAAGG
AGCAGGACTGGGGCTTTCTATAGCTTTAGAAGCAGTAAAGCTTCATGGTGGAGAGTTATATGCAGTAATGAAAAATGAAA
GATTGGGAATTATAATAAAATTATAA

Upstream 100 bases:

>100_bases
AATAGCTTGTTGACCCATATTAGAAATTTAAGAGAAAAGCTAAGTGATACTGTAAAAAACTCAAAATACATTAAAACTGT
TTGGGGAGTGGGTTATAAGA

Downstream 100 bases:

>100_bases
TAGCATATATAGTATAGAAGGGTTTTCAGGAAAAGGGGACCTTTCTTTTTTATTTAAAAATCATAATAAAAAAATTAATA
AATTGTTAACAATGATAAAT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKRKLRIALTTELMLLFVISLIITIFGMMMVLGSLNISSYNINKFLFPEHFKDKVIIEEYEKTVGRRVDRVIKGNINQLK
VPHKMDEKLKKEFPYNFRGKTLVYIINSNGEILSSNNEENMYTIRKSTIKEGLELKRYDDNIIINIRETKKISDNLYIVI
NNDSVIDGDFIIMCTGLFAFMIIFAILAKGRLKYIISIKKGVNKLYSSDFEDKIPLKYNNELTSLAIALNDMGGKIKENK
ENEKEFLLNISHDLRTPLTSILGFLNLLKQKKYDIEEEREGYINTIEEQSLYLKTLIDEFFQFSTLKWKNVKLNKENINL
QEILRQISDGFYPQLKENKISVSMNFPEKSLYKEVDVDKFMRVLENVISNAIKYSHKGTEISLNLYENKDKIVMEFWNTP
IEALKEDELDFLFKRFYKKDLSRGNKGAGLGLSIALEAVKLHGGELYAVMKNERLGIIIKL

Sequences:

>Translated_461_residues
MKRKLRIALTTELMLLFVISLIITIFGMMMVLGSLNISSYNINKFLFPEHFKDKVIIEEYEKTVGRRVDRVIKGNINQLK
VPHKMDEKLKKEFPYNFRGKTLVYIINSNGEILSSNNEENMYTIRKSTIKEGLELKRYDDNIIINIRETKKISDNLYIVI
NNDSVIDGDFIIMCTGLFAFMIIFAILAKGRLKYIISIKKGVNKLYSSDFEDKIPLKYNNELTSLAIALNDMGGKIKENK
ENEKEFLLNISHDLRTPLTSILGFLNLLKQKKYDIEEEREGYINTIEEQSLYLKTLIDEFFQFSTLKWKNVKLNKENINL
QEILRQISDGFYPQLKENKISVSMNFPEKSLYKEVDVDKFMRVLENVISNAIKYSHKGTEISLNLYENKDKIVMEFWNTP
IEALKEDELDFLFKRFYKKDLSRGNKGAGLGLSIALEAVKLHGGELYAVMKNERLGIIIKL
>Mature_461_residues
MKRKLRIALTTELMLLFVISLIITIFGMMMVLGSLNISSYNINKFLFPEHFKDKVIIEEYEKTVGRRVDRVIKGNINQLK
VPHKMDEKLKKEFPYNFRGKTLVYIINSNGEILSSNNEENMYTIRKSTIKEGLELKRYDDNIIINIRETKKISDNLYIVI
NNDSVIDGDFIIMCTGLFAFMIIFAILAKGRLKYIISIKKGVNKLYSSDFEDKIPLKYNNELTSLAIALNDMGGKIKENK
ENEKEFLLNISHDLRTPLTSILGFLNLLKQKKYDIEEEREGYINTIEEQSLYLKTLIDEFFQFSTLKWKNVKLNKENINL
QEILRQISDGFYPQLKENKISVSMNFPEKSLYKEVDVDKFMRVLENVISNAIKYSHKGTEISLNLYENKDKIVMEFWNTP
IEALKEDELDFLFKRFYKKDLSRGNKGAGLGLSIALEAVKLHGGELYAVMKNERLGIIIKL

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=265, Percent_Identity=25.2830188679245, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786783, Length=291, Percent_Identity=27.1477663230241, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1788713, Length=209, Percent_Identity=29.1866028708134, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786912, Length=239, Percent_Identity=25.9414225941423, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790346, Length=231, Percent_Identity=25.5411255411255, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI48994928, Length=206, Percent_Identity=26.2135922330097, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693157, Length=222, Percent_Identity=23.8738738738739, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788279, Length=284, Percent_Identity=26.7605633802817, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790861, Length=276, Percent_Identity=22.8260869565217, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789149, Length=224, Percent_Identity=27.6785714285714, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790551, Length=236, Percent_Identity=24.5762711864407, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786600, Length=224, Percent_Identity=25, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87082128, Length=210, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53671; Mature: 53671

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKLRIALTTELMLLFVISLIITIFGMMMVLGSLNISSYNINKFLFPEHFKDKVIIEEY
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEECCHHCCCCHHHHHH
EKTVGRRVDRVIKGNINQLKVPHKMDEKLKKEFPYNFRGKTLVYIINSNGEILSSNNEEN
HHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCCCCC
MYTIRKSTIKEGLELKRYDDNIIINIRETKKISDNLYIVINNDSVIDGDFIIMCTGLFAF
EEEEHHHHHHCCCEEEECCCCEEEEEECCCCCCCCEEEEECCCCEECCCHHHHHHHHHHH
MIIFAILAKGRLKYIISIKKGVNKLYSSDFEDKIPLKYNNELTSLAIALNDMGGKIKENK
HHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCCCCCEEECCCHHEEEEEEECCCCCCCCCC
ENEKEFLLNISHDLRTPLTSILGFLNLLKQKKYDIEEEREGYINTIEEQSLYLKTLIDEF
CCCHHHEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHH
FQFSTLKWKNVKLNKENINLQEILRQISDGFYPQLKENKISVSMNFPEKSLYKEVDVDKF
HHHCCEEEECEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHCCHHHH
MRVLENVISNAIKYSHKGTEISLNLYENKDKIVMEFWNTPIEALKEDELDFLFKRFYKKD
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEHHCCCHHHHHHHHHHHHHHHHHHHH
LSRGNKGAGLGLSIALEAVKLHGGELYAVMKNERLGIIIKL
HCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCEEEEEEC
>Mature Secondary Structure
MKRKLRIALTTELMLLFVISLIITIFGMMMVLGSLNISSYNINKFLFPEHFKDKVIIEEY
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEECCHHCCCCHHHHHH
EKTVGRRVDRVIKGNINQLKVPHKMDEKLKKEFPYNFRGKTLVYIINSNGEILSSNNEEN
HHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCCCCC
MYTIRKSTIKEGLELKRYDDNIIINIRETKKISDNLYIVINNDSVIDGDFIIMCTGLFAF
EEEEHHHHHHCCCEEEECCCCEEEEEECCCCCCCCEEEEECCCCEECCCHHHHHHHHHHH
MIIFAILAKGRLKYIISIKKGVNKLYSSDFEDKIPLKYNNELTSLAIALNDMGGKIKENK
HHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCCCCCEEECCCHHEEEEEEECCCCCCCCCC
ENEKEFLLNISHDLRTPLTSILGFLNLLKQKKYDIEEEREGYINTIEEQSLYLKTLIDEF
CCCHHHEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHH
FQFSTLKWKNVKLNKENINLQEILRQISDGFYPQLKENKISVSMNFPEKSLYKEVDVDKF
HHHCCEEEECEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHCCHHHH
MRVLENVISNAIKYSHKGTEISLNLYENKDKIVMEFWNTPIEALKEDELDFLFKRFYKKD
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEHHCCCHHHHHHHHHHHHHHHHHHHH
LSRGNKGAGLGLSIALEAVKLHGGELYAVMKNERLGIIIKL
HCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]