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The map label for this gene is tyrS

Identifier: 148381266

GI number: 148381266

Start: 3541467

End: 3542690

Strand: Reverse

Name: tyrS

Synonym: CBO3323

Alternate gene names: 148381266

Gene position: 3542690-3541467 (Counterclockwise)

Preceding gene: 148381267

Following gene: 148381261

Centisome position: 91.14

GC content: 30.56

Gene sequence:

>1224_bases
TTGAGTAATGTATATGACATATTAAAAGAACGTGGTTATATAAAACAATTAACACATGAAGAAGAAATAAGAGAACTTTT
AGGTAAAGAAAAAATTTCTTTTTATATAGGTTTTGATCCTACAGCAGACAGCTTACATGTAGGTCACTTCCTTCAAATGA
TGGTTATGGCTCATATGCAAAAGGCTGGACATAGACCTATAGCATTAGTAGGTGGAGGAACAGGAATGATAGGGGATCCT
ACTGGTAAAACAGATATGAGAAAGATGATGACAAAAGAGCAAATAGAGCATAACTGTAACTGTTTTAAAAAGCAATTAGC
AAAAATAATAGATTTTTCAGAAGATAAAGCTATTATGGTTAATAATGCAGATTGGCTTTTAAATTTAAATTACATAGAAT
TCTTAAGGGAAATAGGAGTTCATTTTTCTGTAAATAAAATGTTAACAGCAGAATGTTTTAAGAGTAGATTAGAAAAAGGG
TTATCTTTTTTAGAATTTAATTATATGTTAATGCAAGGTTATGATTTCTTAGAATTAAATAGAAAATATAACTGCGTTAT
GGAATTAGGTGGAGATGACCAATGGTCTAATATATTAGCAGGTGTAGATTTGATAAGAAGAAAAGAAAGCAAATCAGCCT
ATGGTATGACATTTACTCTTTTAACTAATAGTGAAGGAAAGAAAATGGGTAAAACAGAAAGTGGTGCCCTATGGTTAGAT
CCAGAAAAAACATCACCATATGAATTCTATCAATATTGGAGAAATGTAGCAGATGCAGATGTAGAAAAATGTTTACGTCT
AATAACTTTCTTACCAATGGATGAAGTTAGAAGACTTTCATCATTAGAAGGAGCAGAAATAAATGAAGCTAAAAAGGTTT
TAGCTTTTGAAGTTACTAAGCTTATACATGGAGAAGAAGAAGCTCAAAAAGCTAAAATTGCTGCAGAAGCTTTATTCGGT
GGAAATGCTAAAGATTTAGGAAATATGCCTACAGCTTATATAGATAAGAATGATTTAAATAATCTATTAGTAGATCTATT
AGTTAAGTGTGAAATATTGCCATCTAAGAGTGAAGCCAGGAGACTAATAAAGCAGGGTGGTCTTTATTTAAATGATGAGA
AAGTTACAGATATGAATTTAGTAGTAACAGAAGAACATGTGACAGAAGATGGAATAATGATTAGAAGAGGCAAAAAGAAT
TTTAACAGAATAGTTGTAGAGTAG

Upstream 100 bases:

>100_bases
TAAGATTTAAATTATGTAAAACATTGCTTATATGAATATTGATAAAATATAACTTTCAATATATACTAAAAAAAGGAACT
AATATTAGGAGGAATGAACA

Downstream 100 bases:

>100_bases
TGAATAAAAGAGACTATCTTAAAATAGCTTTAATTTTAAAAGCGTAAATATAAATAATATATTCATAAAGCACTTCATTT
TGCTAAGCGGTTCTAGATTT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSNVYDILKERGYIKQLTHEEEIRELLGKEKISFYIGFDPTADSLHVGHFLQMMVMAHMQKAGHRPIALVGGGTGMIGDP
TGKTDMRKMMTKEQIEHNCNCFKKQLAKIIDFSEDKAIMVNNADWLLNLNYIEFLREIGVHFSVNKMLTAECFKSRLEKG
LSFLEFNYMLMQGYDFLELNRKYNCVMELGGDDQWSNILAGVDLIRRKESKSAYGMTFTLLTNSEGKKMGKTESGALWLD
PEKTSPYEFYQYWRNVADADVEKCLRLITFLPMDEVRRLSSLEGAEINEAKKVLAFEVTKLIHGEEEAQKAKIAAEALFG
GNAKDLGNMPTAYIDKNDLNNLLVDLLVKCEILPSKSEARRLIKQGGLYLNDEKVTDMNLVVTEEHVTEDGIMIRRGKKN
FNRIVVE

Sequences:

>Translated_407_residues
MSNVYDILKERGYIKQLTHEEEIRELLGKEKISFYIGFDPTADSLHVGHFLQMMVMAHMQKAGHRPIALVGGGTGMIGDP
TGKTDMRKMMTKEQIEHNCNCFKKQLAKIIDFSEDKAIMVNNADWLLNLNYIEFLREIGVHFSVNKMLTAECFKSRLEKG
LSFLEFNYMLMQGYDFLELNRKYNCVMELGGDDQWSNILAGVDLIRRKESKSAYGMTFTLLTNSEGKKMGKTESGALWLD
PEKTSPYEFYQYWRNVADADVEKCLRLITFLPMDEVRRLSSLEGAEINEAKKVLAFEVTKLIHGEEEAQKAKIAAEALFG
GNAKDLGNMPTAYIDKNDLNNLLVDLLVKCEILPSKSEARRLIKQGGLYLNDEKVTDMNLVVTEEHVTEDGIMIRRGKKN
FNRIVVE
>Mature_406_residues
SNVYDILKERGYIKQLTHEEEIRELLGKEKISFYIGFDPTADSLHVGHFLQMMVMAHMQKAGHRPIALVGGGTGMIGDPT
GKTDMRKMMTKEQIEHNCNCFKKQLAKIIDFSEDKAIMVNNADWLLNLNYIEFLREIGVHFSVNKMLTAECFKSRLEKGL
SFLEFNYMLMQGYDFLELNRKYNCVMELGGDDQWSNILAGVDLIRRKESKSAYGMTFTLLTNSEGKKMGKTESGALWLDP
EKTSPYEFYQYWRNVADADVEKCLRLITFLPMDEVRRLSSLEGAEINEAKKVLAFEVTKLIHGEEEAQKAKIAAEALFGG
NAKDLGNMPTAYIDKNDLNNLLVDLLVKCEILPSKSEARRLIKQGGLYLNDEKVTDMNLVVTEEHVTEDGIMIRRGKKNF
NRIVVE

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=427, Percent_Identity=33.2552693208431, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI1787925, Length=419, Percent_Identity=43.9140811455847, Blast_Score=360, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25145802, Length=398, Percent_Identity=34.9246231155779, Blast_Score=217, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI25145805, Length=293, Percent_Identity=34.4709897610922, Blast_Score=157, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6325160, Length=434, Percent_Identity=32.0276497695853, Blast_Score=233, Evalue=6e-62,
Organism=Drosophila melanogaster, GI20130383, Length=428, Percent_Identity=37.8504672897196, Blast_Score=301, Evalue=8e-82,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_CLOB1 (A7FYR9)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385642.1
- ProteinModelPortal:   A7FYR9
- SMR:   A7FYR9
- STRING:   A7FYR9
- GeneID:   5396495
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3381
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   TFYIGFD
- ProtClustDB:   PRK05912
- BioCyc:   CBOT441770:CLB_3381-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 46459; Mature: 46328

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 35-35 BINDING 168-168 BINDING 172-172 BINDING 231-231

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVYDILKERGYIKQLTHEEEIRELLGKEKISFYIGFDPTADSLHVGHFLQMMVMAHMQ
CCHHHHHHHCCCHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
KAGHRPIALVGGGTGMIGDPTGKTDMRKMMTKEQIEHNCNCFKKQLAKIIDFSEDKAIMV
HCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
NNADWLLNLNYIEFLREIGVHFSVNKMLTAECFKSRLEKGLSFLEFNYMLMQGYDFLELN
ECCCEEEECHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEECC
RKYNCVMELGGDDQWSNILAGVDLIRRKESKSAYGMTFTLLTNSEGKKMGKTESGALWLD
CCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEC
PEKTSPYEFYQYWRNVADADVEKCLRLITFLPMDEVRRLSSLEGAEINEAKKVLAFEVTK
CCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LIHGEEEAQKAKIAAEALFGGNAKDLGNMPTAYIDKNDLNNLLVDLLVKCEILPSKSEAR
HHCCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEECHHHHHHHHHHHHHHHHCCCCHHHHH
RLIKQGGLYLNDEKVTDMNLVVTEEHVTEDGIMIRRGKKNFNRIVVE
HHHHHCCCEECCCCCCCEEEEEECCCCCCCCEEEEECCCCCCEEECC
>Mature Secondary Structure 
SNVYDILKERGYIKQLTHEEEIRELLGKEKISFYIGFDPTADSLHVGHFLQMMVMAHMQ
CHHHHHHHCCCHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
KAGHRPIALVGGGTGMIGDPTGKTDMRKMMTKEQIEHNCNCFKKQLAKIIDFSEDKAIMV
HCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
NNADWLLNLNYIEFLREIGVHFSVNKMLTAECFKSRLEKGLSFLEFNYMLMQGYDFLELN
ECCCEEEECHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEECC
RKYNCVMELGGDDQWSNILAGVDLIRRKESKSAYGMTFTLLTNSEGKKMGKTESGALWLD
CCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEC
PEKTSPYEFYQYWRNVADADVEKCLRLITFLPMDEVRRLSSLEGAEINEAKKVLAFEVTK
CCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LIHGEEEAQKAKIAAEALFGGNAKDLGNMPTAYIDKNDLNNLLVDLLVKCEILPSKSEAR
HHCCHHHHHHHHHHHHHHHCCCCHHHCCCCCEEECHHHHHHHHHHHHHHHHCCCCHHHHH
RLIKQGGLYLNDEKVTDMNLVVTEEHVTEDGIMIRRGKKNFNRIVVE
HHHHHCCCEECCCCCCCEEEEEECCCCCCCCEEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA