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The map label for this gene is braB [H]

Identifier: 148381237

GI number: 148381237

Start: 3502001

End: 3503323

Strand: Reverse

Name: braB [H]

Synonym: CBO3294

Alternate gene names: 148381237

Gene position: 3503323-3502001 (Counterclockwise)

Preceding gene: 148381238

Following gene: 148381236

Centisome position: 90.13

GC content: 33.41

Gene sequence:

>1323_bases
ATGTCAGAACAAAAAAGACGAAAGGATATTTTTGTACAAGGATTCGCACTATTCGCAATTTTTTTCGGAGCAGGAAACTT
AATTTTTCCACCTAGTTTAGGAATGGCCGCAGGAGATAATTGGGCTCTAGCTGCTTTTGGTTTTTTACTTACAGATCCGG
TACTGCCGATCTTAGGTGTAATTGCAACAGCTAAAGTAGGGGGGAAAGCAGAAGACTTAGGTAAGAGAGTTAGTCCAGGC
TTTGCAAAGGTTTTAGGAGCTGTCTGTATTTTGGTTATTGGTCCGTTGTTTGCTATTCCTAGAACAGCGGCTACTGTATA
TGAAATAGCAGTAAAACCAGTAGCACCTGGTGTGCCGATCATAGTTATATCACTAATATTTTTTGTGTTAACCTATATAT
TTGTAAGTAATCAGGGGCATGTAATAGATAATATCGGTAAAATTTTAACACCATTGTTATTAATTATACTAGCTGCCATA
GTAATTGCAAGTATAGTAACACCTGTAGGGCCAATAGTAGCAACAAAACCTAATAGATACTTTTTAATAGGTTTTCAGGA
AGGGTATCAAACAATGGATGCGTTGGGAGCATCATTAATGGCTGGAATAGTATTAACAGATTTAATTTTAAAAGGATATA
AAGATAGAAAAGAACAAGAATATATGACAATTAGGGTAGGCTTAGTTTCAGGAGTTCTATTATTATTGGTATATGGAGGT
CTTACATTTGTAGGAGCTACTGGAAGTGGAATTTTTACCGGAGATATTAGTAGAGTTGATCTATTATTAAAATTAGTATA
TGGACTTTTCGGAAATGCTGGTGCTGTAGCTATATCCATAGCAGTAGCTTTAGCCTGTTTAACTACAGCAGTAGGACTTA
CTTCTACAGCAGCTAATTTTTTTAATAGTGTAAGTAATGGGAAGCTTTCTTACAAAGCTGTTAGTATTGCTGTAGTATTA
TGTAGTTTATTTTTATCTGTAATAGGGGTAGAAGGATTAATCAATATAGCTGTACCAATTTTATCTACTGTATACCCAGT
TATGATTGTTTTAATATTAATGACATTATTTGATAGACACATTCCTAATAATTTAACTTATACTGGTGCTGTAATAGGAG
CATTTTTAGTTAGTTTAATTATAAATATAAATAGTGCATTTGGAATTTTAAATGGTCCAATGGCGCTTGTGAAAAAATTA
CCTCTTTTTGAAGCGGGATTTCCATGGATTGTACCAGCTATAGTATTGGCTATTTTATTCACTATATTTGGGAAAAAAAA
TAAATCCCAAAGCTTAGAACATGAAAACTCTTTTGATAATTAA

Upstream 100 bases:

>100_bases
TAAATTTTATTGAAATTCTGAATAGTTGCTATATTGACTACAATTTATTTATGTGATACATTGATAAAGAATAAACAATT
TAAAAAAAGGAGTGTGAAAA

Downstream 100 bases:

>100_bases
CCTACGTTGATCTGCTAAATCGTAAAAGTAATAAAATAGTAATGAAAAAAGCAATTATAGAGATATTAAAGTTTTATGGA
AAATTATAGGCAGATAGTTT

Product: branched chain amino acid ABC transporter carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSEQKRRKDIFVQGFALFAIFFGAGNLIFPPSLGMAAGDNWALAAFGFLLTDPVLPILGVIATAKVGGKAEDLGKRVSPG
FAKVLGAVCILVIGPLFAIPRTAATVYEIAVKPVAPGVPIIVISLIFFVLTYIFVSNQGHVIDNIGKILTPLLLIILAAI
VIASIVTPVGPIVATKPNRYFLIGFQEGYQTMDALGASLMAGIVLTDLILKGYKDRKEQEYMTIRVGLVSGVLLLLVYGG
LTFVGATGSGIFTGDISRVDLLLKLVYGLFGNAGAVAISIAVALACLTTAVGLTSTAANFFNSVSNGKLSYKAVSIAVVL
CSLFLSVIGVEGLINIAVPILSTVYPVMIVLILMTLFDRHIPNNLTYTGAVIGAFLVSLIININSAFGILNGPMALVKKL
PLFEAGFPWIVPAIVLAILFTIFGKKNKSQSLEHENSFDN

Sequences:

>Translated_440_residues
MSEQKRRKDIFVQGFALFAIFFGAGNLIFPPSLGMAAGDNWALAAFGFLLTDPVLPILGVIATAKVGGKAEDLGKRVSPG
FAKVLGAVCILVIGPLFAIPRTAATVYEIAVKPVAPGVPIIVISLIFFVLTYIFVSNQGHVIDNIGKILTPLLLIILAAI
VIASIVTPVGPIVATKPNRYFLIGFQEGYQTMDALGASLMAGIVLTDLILKGYKDRKEQEYMTIRVGLVSGVLLLLVYGG
LTFVGATGSGIFTGDISRVDLLLKLVYGLFGNAGAVAISIAVALACLTTAVGLTSTAANFFNSVSNGKLSYKAVSIAVVL
CSLFLSVIGVEGLINIAVPILSTVYPVMIVLILMTLFDRHIPNNLTYTGAVIGAFLVSLIININSAFGILNGPMALVKKL
PLFEAGFPWIVPAIVLAILFTIFGKKNKSQSLEHENSFDN
>Mature_439_residues
SEQKRRKDIFVQGFALFAIFFGAGNLIFPPSLGMAAGDNWALAAFGFLLTDPVLPILGVIATAKVGGKAEDLGKRVSPGF
AKVLGAVCILVIGPLFAIPRTAATVYEIAVKPVAPGVPIIVISLIFFVLTYIFVSNQGHVIDNIGKILTPLLLIILAAIV
IASIVTPVGPIVATKPNRYFLIGFQEGYQTMDALGASLMAGIVLTDLILKGYKDRKEQEYMTIRVGLVSGVLLLLVYGGL
TFVGATGSGIFTGDISRVDLLLKLVYGLFGNAGAVAISIAVALACLTTAVGLTSTAANFFNSVSNGKLSYKAVSIAVVLC
SLFLSVIGVEGLINIAVPILSTVYPVMIVLILMTLFDRHIPNNLTYTGAVIGAFLVSLIININSAFGILNGPMALVKKLP
LFEAGFPWIVPAIVLAILFTIFGKKNKSQSLEHENSFDN

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=444, Percent_Identity=34.2342342342342, Blast_Score=226, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46478; Mature: 46347

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEQKRRKDIFVQGFALFAIFFGAGNLIFPPSLGMAAGDNWALAAFGFLLTDPVLPILGV
CCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IATAKVGGKAEDLGKRVSPGFAKVLGAVCILVIGPLFAIPRTAATVYEIAVKPVAPGVPI
HHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHH
IVISLIFFVLTYIFVSNQGHVIDNIGKILTPLLLIILAAIVIASIVTPVGPIVATKPNRY
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCE
FLIGFQEGYQTMDALGASLMAGIVLTDLILKGYKDRKEQEYMTIRVGLVSGVLLLLVYGG
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
LTFVGATGSGIFTGDISRVDLLLKLVYGLFGNAGAVAISIAVALACLTTAVGLTSTAANF
HHEEECCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNSVSNGKLSYKAVSIAVVLCSLFLSVIGVEGLINIAVPILSTVYPVMIVLILMTLFDRH
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPNNLTYTGAVIGAFLVSLIININSAFGILNGPMALVKKLPLFEAGFPWIVPAIVLAILF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHH
TIFGKKNKSQSLEHENSFDN
HHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]