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The map label for this gene is yjbJ [H]

Identifier: 148381219

GI number: 148381219

Start: 3480237

End: 3480908

Strand: Reverse

Name: yjbJ [H]

Synonym: CBO3274

Alternate gene names: 148381219

Gene position: 3480908-3480237 (Counterclockwise)

Preceding gene: 148381220

Following gene: 148381218

Centisome position: 89.55

GC content: 33.63

Gene sequence:

>672_bases
ATGAAAGTGAACAGTAGTGAAATGGTATTACAACAGATGCTACAAATGCAATTGATGGGACAGATAATGAAGTCATCCTT
TGGAGATTCATCAAGTTTTCAAATAGTTTTAGATAGTTTGTTAAAGGCCATGGAAAATAAGGATATTAATGCTTCAAATG
ATATATTATCATTAGACAGTGCAGCAAATAGTGGAAATAAATATTATGGAGCAGGACAAAGATTAGAAGATGCGAGAAGA
GAAATAAATAATATAAAATCTGAAGTTAGAACTGGTAATATAACTATAGATAGCGCTGTAGAAAAAGCTTCAAGAAAATA
TGGTATAGACAAAGAACTTCTAATGGCAGTTATAAAGCAAGAATCGGATTTCAATCCTAATTGCGTATCAAGTGCAGGGG
CAAAGGGGTTAATGCAGCTTATGCCAGGTACTGCTAGAGAAGTAGGAGTTACAAATCCCTTCGATATAGAACAAAATATA
GATGGGGGAACAAAATATTTAAAGAAAATGCTAGATATGCATGGAAACGTAAAAGAGTTAGCTTTGGCAGCATATAACGC
AGGTCCTGGAACCCTCCAGTGGAGAGGTGTGAAAAGCCCTTCAGATATAAATAGATTGCCAAGTGAAACAAGAAATTATG
TAAAAAATATAATGAAAAATTATGGAGTATAA

Upstream 100 bases:

>100_bases
TGTGCTTATGAAGATTATGATATATTTAAATTTAAAGTGTAAATAAAGTAGAAAGATGAACGATAATGTTAAATATATGT
TTAATATTGGTGGTGGATTT

Downstream 100 bases:

>100_bases
AAAGGAAAGTGCCCTGCACTTTCCTTTAAATTTGTTATAATGGTAATACTAATAATAGAATAATCAAAACTAGGAGGAAT
TTCATGGAACGTTTAGATAA

Product: Slt family transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MKVNSSEMVLQQMLQMQLMGQIMKSSFGDSSSFQIVLDSLLKAMENKDINASNDILSLDSAANSGNKYYGAGQRLEDARR
EINNIKSEVRTGNITIDSAVEKASRKYGIDKELLMAVIKQESDFNPNCVSSAGAKGLMQLMPGTAREVGVTNPFDIEQNI
DGGTKYLKKMLDMHGNVKELALAAYNAGPGTLQWRGVKSPSDINRLPSETRNYVKNIMKNYGV

Sequences:

>Translated_223_residues
MKVNSSEMVLQQMLQMQLMGQIMKSSFGDSSSFQIVLDSLLKAMENKDINASNDILSLDSAANSGNKYYGAGQRLEDARR
EINNIKSEVRTGNITIDSAVEKASRKYGIDKELLMAVIKQESDFNPNCVSSAGAKGLMQLMPGTAREVGVTNPFDIEQNI
DGGTKYLKKMLDMHGNVKELALAAYNAGPGTLQWRGVKSPSDINRLPSETRNYVKNIMKNYGV
>Mature_223_residues
MKVNSSEMVLQQMLQMQLMGQIMKSSFGDSSSFQIVLDSLLKAMENKDINASNDILSLDSAANSGNKYYGAGQRLEDARR
EINNIKSEVRTGNITIDSAVEKASRKYGIDKELLMAVIKQESDFNPNCVSSAGAKGLMQLMPGTAREVGVTNPFDIEQNI
DGGTKYLKKMLDMHGNVKELALAAYNAGPGTLQWRGVKSPSDINRLPSETRNYVKNIMKNYGV

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=127, Percent_Identity=40.1574803149606, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082191, Length=111, Percent_Identity=36.9369369369369, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 24523; Mature: 24523

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVNSSEMVLQQMLQMQLMGQIMKSSFGDSSSFQIVLDSLLKAMENKDINASNDILSLDS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCC
AANSGNKYYGAGQRLEDARREINNIKSEVRTGNITIDSAVEKASRKYGIDKELLMAVIKQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCHHHHHHHHHH
ESDFNPNCVSSAGAKGLMQLMPGTAREVGVTNPFDIEQNIDGGTKYLKKMLDMHGNVKEL
CCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
ALAAYNAGPGTLQWRGVKSPSDINRLPSETRNYVKNIMKNYGV
HHHHHCCCCCCEEECCCCCCHHHHHCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVNSSEMVLQQMLQMQLMGQIMKSSFGDSSSFQIVLDSLLKAMENKDINASNDILSLDS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCC
AANSGNKYYGAGQRLEDARREINNIKSEVRTGNITIDSAVEKASRKYGIDKELLMAVIKQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCHHHHHHHHHH
ESDFNPNCVSSAGAKGLMQLMPGTAREVGVTNPFDIEQNIDGGTKYLKKMLDMHGNVKEL
CCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
ALAAYNAGPGTLQWRGVKSPSDINRLPSETRNYVKNIMKNYGV
HHHHHCCCCCCEEECCCCCCHHHHHCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]