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The map label for this gene is rluB [H]

Identifier: 148381218

GI number: 148381218

Start: 3479437

End: 3480153

Strand: Reverse

Name: rluB [H]

Synonym: CBO3273

Alternate gene names: 148381218

Gene position: 3480153-3479437 (Counterclockwise)

Preceding gene: 148381219

Following gene: 148381217

Centisome position: 89.54

GC content: 27.62

Gene sequence:

>717_bases
ATGGAACGTTTAGATAAAATACTAGCCAACTTAGGATATGGTACAAGAAAAGAAGTTAAGGCTTTGATTAAAAAAGGGCA
AGTTGATGTTGATGGTGAAGTTGTTAAAGATAATGGTATGCAAATAGATCCAGAAAAAAATAAAATATTTGTATTTGGAA
AAGAATTAGTTTATAAAAAATATATTTATTTAATGATGAATAAACCCCAAGGGGTAGTTTCTGCAACTTTTGATAACTAT
GATGAAACTGTTATAGATTTGTTAGAAGATGAAGACAGAATTTTTGAACCTTTTCCCGTGGGTAGATTAGATAAAAATAC
GGTAGGCTTACTTTTACTTACTAACGATGGAGAGTTAAATCACAGACTTATATCTCCTAAATGGCATGTGGATAAAATAT
ATTATGCCAAAATTAATAAAAAGGTTACTGATGAAGATATAAAGAAATTTAAGAAAGGTATAATATTAGATGATGGTTAT
GAATGTTTTCCTGCAAAGCTTGAAATAATAAAATCATTTAATGAGGAATCAGAAGTAAGAGTTACTATACATGAAGGTAA
ATTTCATCAAGTCAAAAGAATGTTTGAAAGTGTTGATAAAAAGGTTATTTACTTAAAAAGAGAAGAATTTGGACCATTAA
AATTAGATGAGAGCTTAGAAGAGGGTCAATATAGGGAATTAACAGATGAAGAAATATCTATATTAAAAAATCTATAA

Upstream 100 bases:

>100_bases
AAAATTATGGAGTATAAAAAGGAAAGTGCCCTGCACTTTCCTTTAAATTTGTTATAATGGTAATACTAATAATAGAATAA
TCAAAACTAGGAGGAATTTC

Downstream 100 bases:

>100_bases
ATGTAAAAAATATTAAAATATTATGAATATAAAAACATCATTTTAAAAAAAGTTTACAATAAAATTTGCAAAAAAATCTT
GCATTTATCATTTTCATTCA

Product: RNA pseudouridine synthase family protein

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MERLDKILANLGYGTRKEVKALIKKGQVDVDGEVVKDNGMQIDPEKNKIFVFGKELVYKKYIYLMMNKPQGVVSATFDNY
DETVIDLLEDEDRIFEPFPVGRLDKNTVGLLLLTNDGELNHRLISPKWHVDKIYYAKINKKVTDEDIKKFKKGIILDDGY
ECFPAKLEIIKSFNEESEVRVTIHEGKFHQVKRMFESVDKKVIYLKREEFGPLKLDESLEEGQYRELTDEEISILKNL

Sequences:

>Translated_238_residues
MERLDKILANLGYGTRKEVKALIKKGQVDVDGEVVKDNGMQIDPEKNKIFVFGKELVYKKYIYLMMNKPQGVVSATFDNY
DETVIDLLEDEDRIFEPFPVGRLDKNTVGLLLLTNDGELNHRLISPKWHVDKIYYAKINKKVTDEDIKKFKKGIILDDGY
ECFPAKLEIIKSFNEESEVRVTIHEGKFHQVKRMFESVDKKVIYLKREEFGPLKLDESLEEGQYRELTDEEISILKNL
>Mature_238_residues
MERLDKILANLGYGTRKEVKALIKKGQVDVDGEVVKDNGMQIDPEKNKIFVFGKELVYKKYIYLMMNKPQGVVSATFDNY
DETVIDLLEDEDRIFEPFPVGRLDKNTVGLLLLTNDGELNHRLISPKWHVDKIYYAKINKKVTDEDIKKFKKGIILDDGY
ECFPAKLEIIKSFNEESEVRVTIHEGKFHQVKRMFESVDKKVIYLKREEFGPLKLDESLEEGQYRELTDEEISILKNL

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=38.3620689655172, Blast_Score=154, Evalue=4e-39,
Organism=Escherichia coli, GI1787524, Length=248, Percent_Identity=33.0645161290323, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1790453, Length=243, Percent_Identity=29.2181069958848, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI87081838, Length=148, Percent_Identity=33.1081081081081, Blast_Score=81, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27755; Mature: 27755

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLDKILANLGYGTRKEVKALIKKGQVDVDGEVVKDNGMQIDPEKNKIFVFGKELVYKK
CHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEECCCCEECCCCCEEEEEEHHHHHHE
YIYLMMNKPQGVVSATFDNYDETVIDLLEDEDRIFEPFPVGRLDKNTVGLLLLTNDGELN
EEEEEECCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
HRLISPKWHVDKIYYAKINKKVTDEDIKKFKKGIILDDGYECFPAKLEIIKSFNEESEVR
CEEECCCCCCCEEEEEECCCCCCHHHHHHHHCCCEECCCCCCCHHHHHHHHHCCCCCEEE
VTIHEGKFHQVKRMFESVDKKVIYLKREEFGPLKLDESLEEGQYRELTDEEISILKNL
EEEECCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MERLDKILANLGYGTRKEVKALIKKGQVDVDGEVVKDNGMQIDPEKNKIFVFGKELVYKK
CHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEECCCCEECCCCCEEEEEEHHHHHHE
YIYLMMNKPQGVVSATFDNYDETVIDLLEDEDRIFEPFPVGRLDKNTVGLLLLTNDGELN
EEEEEECCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
HRLISPKWHVDKIYYAKINKKVTDEDIKKFKKGIILDDGYECFPAKLEIIKSFNEESEVR
CEEECCCCCCCEEEEEECCCCCCHHHHHHHHCCCEECCCCCCCHHHHHHHHHCCCCCEEE
VTIHEGKFHQVKRMFESVDKKVIYLKREEFGPLKLDESLEEGQYRELTDEEISILKNL
EEEECCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]