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The map label for this gene is pflA [H]

Identifier: 148381164

GI number: 148381164

Start: 3424102

End: 3426330

Strand: Reverse

Name: pflA [H]

Synonym: CBO3216

Alternate gene names: 148381164

Gene position: 3426330-3424102 (Counterclockwise)

Preceding gene: 148381165

Following gene: 148381163

Centisome position: 88.15

GC content: 29.92

Gene sequence:

>2229_bases
ATGTTTTATGATTCATGGAAAAAATTTAATGAAGGTTCATGGGAAAATAATATAAATGTAAGAGAGTTTATACAAAATAA
TTATACGCCTTATTATGGAGATCATAGCTTTTTAAAGGAATCTACTGAAAAAACGAAAGATCTTTGGAAACAATGCGAAA
CATTAATAGCAGAAGAAATAAAAAAAGGAATATTAGATGTAGATTTAGATAATATTTCTGCTATAAATGCTTTTGATGCA
GGATATATAGACAAGGATAATGAAACCATAGTAGGTCTTCAGACAGATAAACCACTAAAAAGAATTATAAATCCTTTTGG
TGGTATAAGAATGGTAAAACAAGCTTTAGAAGCCTATGATTATAAGTTAAACCCACACATAAAGGATATATTTACCAAAT
ATAGAAAAACCCATAATGATGGTGTTTTTGATGCTTATACTGAAGAGATGAGAAAAGCCAGAAGTGCAGGATTATTAACA
GGGCTTCCAGATGCCTATGGAAGAGGGAGAATAATAGGTGATTATAGAAGAATACCATTATATGGGGTAGATTTTTTAAT
AAAAAATAAAGAAGAAGATTTAAAAGCTGTTAAAGGGGAAATGAATGAAACTACCATAAGAAAAAGAGAAGAGATAAGCG
AACAAATAAAAGCTTTAATAGCTATGAAAGAAATGGCACTAAAGTATGGAATAGACATAAGCAAGCCAGCTAAAAATGCA
GAAGAAGCAGTACAATTTTTATATTTTGGTTATTTAGCAGGAGTTAAAGAAAATAATGGAGCAGCGATGTCCTTAGGAAG
AGTAAGTTCATTTATAGATATATATATAGAAAGAGACTTGAAACAGGGAATACTAACAGAAGAAACAGCGCAAGAAATTA
TAGATCAGTTTGTTATAAAATTAAGATTAGTAAGACATCTTAGAACACCAGAGTATAATGATCTTTTTGCAGGAGATCCT
AACTGGATTACAGAAGCTATAGGGGGAATGGGATTAAATGGGGAGACTTTAGTTACTAAAACATCCTATAGATTCTTAAA
TACTTTAAATAATTTAGGACCAGCACCAGAACCTAATATGACGATATTATGGTCACAAAACTTACCAGAAAACTTTAAAA
AATTCTGTGCAGAAATGTCTATAAAGACAGATTCTATTCAATATGAAAATGACGATTTAATGAGAGATATATATGGAGAT
GATTATGGTATAGCTTGTTGTGTATCAGCTATGGCCTTGGGAAAACAGATGCAATTTTTCGGTGCAAGATGCAATTTAGC
CAAAGCTTTATTGTATTCCATAAATGGTGGAGTAGATGAAAAGAAGAATATTAAAGTAATAGACAATATAAATGCAATAG
AAGATACTATGTTAGATTATGAAAAAGTTAAGGAAAATTATTTTAAAGTATTAGAATATATAGCAAATTTATATGTAAAT
ACTATGAATATAATACATTATATGCATGATAAGTATGCTTATGAAGGTGGGCTTATGGCACTTCATGATACAGAAGTAGA
AAGACTTATGGCCTTTGGTGTAGCTGGATTGTCTGTTGTAGCAGATTCATTAAGTGCTATAAAATATGCAAAAGTGAAAC
CAGTAAGAGAAAATGGTATTGCTGTAGATTTTGAAATAGAAGGAGATTTCCCTAAATACGGTAATGATGATGATAGAGTT
GATGAAATAGCAGTGGAAATAGTTAATAAATTTATTAATGAATTAAAGAAAAATAAAACTTATAGAAATGCAAAACATAC
ACTTTCAGTTTTAACAATAACTTCTAATGTAGTGTATGGTAAGAAAACAGGTTCAACACCAGATGGAAGAAAATCAGGAG
AAGCATTTGCTCCAGGTGCTAATCCTATGCATGGAAGAGATAAAAATGGTGCTTTAGCATCATTAAATTCTGTAGCTAAA
ATACCTTACAAAAATATTTGTGAAGATGGAGTATCAAATACATTTTCTATAGTTCCAGATGCATTAGGTAAAAGTGAAGA
AGAAAGAATAAATAATTTAGTATCTATATTAGATGGATATTTTGTACAAAATGCTCATCATTTAAATGTTAATGTGTTAA
ACAGAGATCTACTAATAGATGCAATGGAACATCCAGAAAAATATCCATCACTTACTATAAGGGTATCAGGTTATGCAGTT
CATTTTAATAGATTGACTAAAGCACAACAATTAGAAGTGATAAGCAGAACTTTCCATAAAGACATGTAA

Upstream 100 bases:

>100_bases
TAGGAGTAATTGTGGTTTGCAAATTAAAGTTAGACGTGGTTTTTTAGGCAACATAAGCTCTAAATGGCTTTAAAATATAT
TAAATTTTAGGAGGTTTGCT

Downstream 100 bases:

>100_bases
ATTATTTCTATATTATGATTATTAGGATTAATGTTTCTAGTCTTTTTAGATGAGATATATTATAGGTTTTTATGCTGTAT
ATAAGGGGTGTCATAGCCCT

Product: formate acetyltransferase 1

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 742; Mature: 742

Protein sequence:

>742_residues
MFYDSWKKFNEGSWENNINVREFIQNNYTPYYGDHSFLKESTEKTKDLWKQCETLIAEEIKKGILDVDLDNISAINAFDA
GYIDKDNETIVGLQTDKPLKRIINPFGGIRMVKQALEAYDYKLNPHIKDIFTKYRKTHNDGVFDAYTEEMRKARSAGLLT
GLPDAYGRGRIIGDYRRIPLYGVDFLIKNKEEDLKAVKGEMNETTIRKREEISEQIKALIAMKEMALKYGIDISKPAKNA
EEAVQFLYFGYLAGVKENNGAAMSLGRVSSFIDIYIERDLKQGILTEETAQEIIDQFVIKLRLVRHLRTPEYNDLFAGDP
NWITEAIGGMGLNGETLVTKTSYRFLNTLNNLGPAPEPNMTILWSQNLPENFKKFCAEMSIKTDSIQYENDDLMRDIYGD
DYGIACCVSAMALGKQMQFFGARCNLAKALLYSINGGVDEKKNIKVIDNINAIEDTMLDYEKVKENYFKVLEYIANLYVN
TMNIIHYMHDKYAYEGGLMALHDTEVERLMAFGVAGLSVVADSLSAIKYAKVKPVRENGIAVDFEIEGDFPKYGNDDDRV
DEIAVEIVNKFINELKKNKTYRNAKHTLSVLTITSNVVYGKKTGSTPDGRKSGEAFAPGANPMHGRDKNGALASLNSVAK
IPYKNICEDGVSNTFSIVPDALGKSEEERINNLVSILDGYFVQNAHHLNVNVLNRDLLIDAMEHPEKYPSLTIRVSGYAV
HFNRLTKAQQLEVISRTFHKDM

Sequences:

>Translated_742_residues
MFYDSWKKFNEGSWENNINVREFIQNNYTPYYGDHSFLKESTEKTKDLWKQCETLIAEEIKKGILDVDLDNISAINAFDA
GYIDKDNETIVGLQTDKPLKRIINPFGGIRMVKQALEAYDYKLNPHIKDIFTKYRKTHNDGVFDAYTEEMRKARSAGLLT
GLPDAYGRGRIIGDYRRIPLYGVDFLIKNKEEDLKAVKGEMNETTIRKREEISEQIKALIAMKEMALKYGIDISKPAKNA
EEAVQFLYFGYLAGVKENNGAAMSLGRVSSFIDIYIERDLKQGILTEETAQEIIDQFVIKLRLVRHLRTPEYNDLFAGDP
NWITEAIGGMGLNGETLVTKTSYRFLNTLNNLGPAPEPNMTILWSQNLPENFKKFCAEMSIKTDSIQYENDDLMRDIYGD
DYGIACCVSAMALGKQMQFFGARCNLAKALLYSINGGVDEKKNIKVIDNINAIEDTMLDYEKVKENYFKVLEYIANLYVN
TMNIIHYMHDKYAYEGGLMALHDTEVERLMAFGVAGLSVVADSLSAIKYAKVKPVRENGIAVDFEIEGDFPKYGNDDDRV
DEIAVEIVNKFINELKKNKTYRNAKHTLSVLTITSNVVYGKKTGSTPDGRKSGEAFAPGANPMHGRDKNGALASLNSVAK
IPYKNICEDGVSNTFSIVPDALGKSEEERINNLVSILDGYFVQNAHHLNVNVLNRDLLIDAMEHPEKYPSLTIRVSGYAV
HFNRLTKAQQLEVISRTFHKDM
>Mature_742_residues
MFYDSWKKFNEGSWENNINVREFIQNNYTPYYGDHSFLKESTEKTKDLWKQCETLIAEEIKKGILDVDLDNISAINAFDA
GYIDKDNETIVGLQTDKPLKRIINPFGGIRMVKQALEAYDYKLNPHIKDIFTKYRKTHNDGVFDAYTEEMRKARSAGLLT
GLPDAYGRGRIIGDYRRIPLYGVDFLIKNKEEDLKAVKGEMNETTIRKREEISEQIKALIAMKEMALKYGIDISKPAKNA
EEAVQFLYFGYLAGVKENNGAAMSLGRVSSFIDIYIERDLKQGILTEETAQEIIDQFVIKLRLVRHLRTPEYNDLFAGDP
NWITEAIGGMGLNGETLVTKTSYRFLNTLNNLGPAPEPNMTILWSQNLPENFKKFCAEMSIKTDSIQYENDDLMRDIYGD
DYGIACCVSAMALGKQMQFFGARCNLAKALLYSINGGVDEKKNIKVIDNINAIEDTMLDYEKVKENYFKVLEYIANLYVN
TMNIIHYMHDKYAYEGGLMALHDTEVERLMAFGVAGLSVVADSLSAIKYAKVKPVRENGIAVDFEIEGDFPKYGNDDDRV
DEIAVEIVNKFINELKKNKTYRNAKHTLSVLTITSNVVYGKKTGSTPDGRKSGEAFAPGANPMHGRDKNGALASLNSVAK
IPYKNICEDGVSNTFSIVPDALGKSEEERINNLVSILDGYFVQNAHHLNVNVLNRDLLIDAMEHPEKYPSLTIRVSGYAV
HFNRLTKAQQLEVISRTFHKDM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=751, Percent_Identity=60.8521970705726, Blast_Score=953, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=751, Percent_Identity=59.9201065246338, Blast_Score=941, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=665, Percent_Identity=26.4661654135338, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1790388, Length=730, Percent_Identity=24.1095890410959, Blast_Score=150, Evalue=4e-37,
Organism=Escherichia coli, GI1788933, Length=61, Percent_Identity=63.9344262295082, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84030; Mature: 84030

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYDSWKKFNEGSWENNINVREFIQNNYTPYYGDHSFLKESTEKTKDLWKQCETLIAEEI
CCCCCHHHCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KKGILDVDLDNISAINAFDAGYIDKDNETIVGLQTDKPLKRIINPFGGIRMVKQALEAYD
HHCCEECCCCCCCEECCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHC
YKLNPHIKDIFTKYRKTHNDGVFDAYTEEMRKARSAGLLTGLPDAYGRGRIIGDYRRIPL
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCEECCE
YGVDFLIKNKEEDLKAVKGEMNETTIRKREEISEQIKALIAMKEMALKYGIDISKPAKNA
ECCHHEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
EEAVQFLYFGYLAGVKENNGAAMSLGRVSSFIDIYIERDLKQGILTEETAQEIIDQFVIK
HHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LRLVRHLRTPEYNDLFAGDPNWITEAIGGMGLNGETLVTKTSYRFLNTLNNLGPAPEPNM
HHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCE
TILWSQNLPENFKKFCAEMSIKTDSIQYENDDLMRDIYGDDYGIACCVSAMALGKQMQFF
EEEECCCCCHHHHHHHHHHCCCCCCEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GARCNLAKALLYSINGGVDEKKNIKVIDNINAIEDTMLDYEKVKENYFKVLEYIANLYVN
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TMNIIHYMHDKYAYEGGLMALHDTEVERLMAFGVAGLSVVADSLSAIKYAKVKPVRENGI
HHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
AVDFEIEGDFPKYGNDDDRVDEIAVEIVNKFINELKKNKTYRNAKHTLSVLTITSNVVYG
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCEEEC
KKTGSTPDGRKSGEAFAPGANPMHGRDKNGALASLNSVAKIPYKNICEDGVSNTFSIVPD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCHHHCCH
ALGKSEEERINNLVSILDGYFVQNAHHLNVNVLNRDLLIDAMEHPEKYPSLTIRVSGYAV
HHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHCCCCCCCEEEEEEEEEE
HFNRLTKAQQLEVISRTFHKDM
EHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFYDSWKKFNEGSWENNINVREFIQNNYTPYYGDHSFLKESTEKTKDLWKQCETLIAEEI
CCCCCHHHCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KKGILDVDLDNISAINAFDAGYIDKDNETIVGLQTDKPLKRIINPFGGIRMVKQALEAYD
HHCCEECCCCCCCEECCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHC
YKLNPHIKDIFTKYRKTHNDGVFDAYTEEMRKARSAGLLTGLPDAYGRGRIIGDYRRIPL
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCEECCE
YGVDFLIKNKEEDLKAVKGEMNETTIRKREEISEQIKALIAMKEMALKYGIDISKPAKNA
ECCHHEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
EEAVQFLYFGYLAGVKENNGAAMSLGRVSSFIDIYIERDLKQGILTEETAQEIIDQFVIK
HHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LRLVRHLRTPEYNDLFAGDPNWITEAIGGMGLNGETLVTKTSYRFLNTLNNLGPAPEPNM
HHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCE
TILWSQNLPENFKKFCAEMSIKTDSIQYENDDLMRDIYGDDYGIACCVSAMALGKQMQFF
EEEECCCCCHHHHHHHHHHCCCCCCEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GARCNLAKALLYSINGGVDEKKNIKVIDNINAIEDTMLDYEKVKENYFKVLEYIANLYVN
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TMNIIHYMHDKYAYEGGLMALHDTEVERLMAFGVAGLSVVADSLSAIKYAKVKPVRENGI
HHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
AVDFEIEGDFPKYGNDDDRVDEIAVEIVNKFINELKKNKTYRNAKHTLSVLTITSNVVYG
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCEEEC
KKTGSTPDGRKSGEAFAPGANPMHGRDKNGALASLNSVAKIPYKNICEDGVSNTFSIVPD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCHHHCCH
ALGKSEEERINNLVSILDGYFVQNAHHLNVNVLNRDLLIDAMEHPEKYPSLTIRVSGYAV
HHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHCCCCCCCEEEEEEEEEE
HFNRLTKAQQLEVISRTFHKDM
EHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8636053 [H]