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The map label for this gene is folC [H]

Identifier: 148381111

GI number: 148381111

Start: 3363739

End: 3365028

Strand: Reverse

Name: folC [H]

Synonym: CBO3163

Alternate gene names: 148381111

Gene position: 3365028-3363739 (Counterclockwise)

Preceding gene: 148381112

Following gene: 148381109

Centisome position: 86.57

GC content: 27.83

Gene sequence:

>1290_bases
ATGGATTATAAAGAAGCTAGAGAGTATATACAATCAAAGGCTAAGTTTGGTAGTAATCTAGGCCTAGAAAGAACAGAAAA
GCTTTTAGAGTTATTGGGAAACCCTCATAAGAGGTTAAGATGCATACATATAGCAGGTACAAATGGAAAAGGATCTACTA
CAGCTATGATTTCTGCAGTATTAAAAGAGTCTGGCTATAAAGTTGGAATGTATACATCCCCTTATATAGAGGAGTTTGAA
GAAAGAATACAAATAAATGGCCATAATATAACTAAGGAAGATTTAGGTTATATTATAACTAAGGTAGCTAATATAGTGGA
AAAGGTAGAAAATATGGGATATGGAAATCCTACAGAATTTGAAATTATAACTGTTGCTATGTTTTATTATTTTTGTTTAA
AAGAAGTAGATTTTGCGGTAATAGAAGTAGGTTTAGGGGGACGGTTAGACTCTACTAATGTTTTAGAACCTATTTTAAGT
ATTATAACTTCTATAAGTTATGATCATATGAATATTTTAGGTGAAACCCTAGAAGAAATAACTTATGAAAAAGCAGGAAT
AATAAAAAAAGCTCCTGTTATAACGTATCCACAAAAGAAAGAAGTAGAAAAAAATATAGAAAAGGTGTGTAAGGAAAAAA
ATTGTGAGCTTATAAAAGTAGAAGATAATTTAATAAATATAGAAATAGAAATCATAGAAAAAAATATGGGGCAGCAAAGT
TTTAAATTAAAAACTAAAGAAGATACTTATAATATATGTTTATCCTTATTAGGAGAACATCAAATAAAAAACTGTATTAC
AGTTATATTAGCCTTAGAAAAGTTAATGAAATTAGGTATAAAAATAGAAAAAATACACATAATATCAGCCCTTAAAAAAG
TGAAATGGCCAGCTAGACTAGAAATAGTAAATAAAAATCCTCTAACAGTAATAGACGGAGCCCATAATATAGATGGCATA
GAAAGCTTAAAAAATAATGTGAGTAAATATTTTAAATATAATAAACTTATTTTAATATTAGGCATATTAAAAGATAAACA
AGTAGAAGACATGATAAAAACATTAGTACCACTAGCAGATAGAGTATTAACAGTAGCTCCTCATAATGATAGAGGGGAAA
GTTCAAAAGAATTAATGCACATTGTATTAAAACATAATGAAAGTTGTGAACATCTAGAGGACTATAAAGAATGTTATGAT
AAGGCAAAATTTTACTGTGAAGATGGAGATATGATTTTAATTTGTGGGTCATTATACATGGTAGGTGATATGAGAAAATT
AATAAGGTAA

Upstream 100 bases:

>100_bases
TGTAGTCATTAGGTAATTTCTTATGGCTAAATAGAAATAATATAAAAGTAACAGAAGGGTTATGATTTACGTAAAAGATA
TACATAGGAAGTGATTATAT

Downstream 100 bases:

>100_bases
GGAAAGTGTTAGATGCTTTCCTTATTTTGTTTTTTTGCTTCTTGTAGAGCTTTAGACAATTGATCTGGACAGGAAGTATT
TTTAGAACCACAGGTTATAC

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MDYKEAREYIQSKAKFGSNLGLERTEKLLELLGNPHKRLRCIHIAGTNGKGSTTAMISAVLKESGYKVGMYTSPYIEEFE
ERIQINGHNITKEDLGYIITKVANIVEKVENMGYGNPTEFEIITVAMFYYFCLKEVDFAVIEVGLGGRLDSTNVLEPILS
IITSISYDHMNILGETLEEITYEKAGIIKKAPVITYPQKKEVEKNIEKVCKEKNCELIKVEDNLINIEIEIIEKNMGQQS
FKLKTKEDTYNICLSLLGEHQIKNCITVILALEKLMKLGIKIEKIHIISALKKVKWPARLEIVNKNPLTVIDGAHNIDGI
ESLKNNVSKYFKYNKLILILGILKDKQVEDMIKTLVPLADRVLTVAPHNDRGESSKELMHIVLKHNESCEHLEDYKECYD
KAKFYCEDGDMILICGSLYMVGDMRKLIR

Sequences:

>Translated_429_residues
MDYKEAREYIQSKAKFGSNLGLERTEKLLELLGNPHKRLRCIHIAGTNGKGSTTAMISAVLKESGYKVGMYTSPYIEEFE
ERIQINGHNITKEDLGYIITKVANIVEKVENMGYGNPTEFEIITVAMFYYFCLKEVDFAVIEVGLGGRLDSTNVLEPILS
IITSISYDHMNILGETLEEITYEKAGIIKKAPVITYPQKKEVEKNIEKVCKEKNCELIKVEDNLINIEIEIIEKNMGQQS
FKLKTKEDTYNICLSLLGEHQIKNCITVILALEKLMKLGIKIEKIHIISALKKVKWPARLEIVNKNPLTVIDGAHNIDGI
ESLKNNVSKYFKYNKLILILGILKDKQVEDMIKTLVPLADRVLTVAPHNDRGESSKELMHIVLKHNESCEHLEDYKECYD
KAKFYCEDGDMILICGSLYMVGDMRKLIR
>Mature_429_residues
MDYKEAREYIQSKAKFGSNLGLERTEKLLELLGNPHKRLRCIHIAGTNGKGSTTAMISAVLKESGYKVGMYTSPYIEEFE
ERIQINGHNITKEDLGYIITKVANIVEKVENMGYGNPTEFEIITVAMFYYFCLKEVDFAVIEVGLGGRLDSTNVLEPILS
IITSISYDHMNILGETLEEITYEKAGIIKKAPVITYPQKKEVEKNIEKVCKEKNCELIKVEDNLINIEIEIIEKNMGQQS
FKLKTKEDTYNICLSLLGEHQIKNCITVILALEKLMKLGIKIEKIHIISALKKVKWPARLEIVNKNPLTVIDGAHNIDGI
ESLKNNVSKYFKYNKLILILGILKDKQVEDMIKTLVPLADRVLTVAPHNDRGESSKELMHIVLKHNESCEHLEDYKECYD
KAKFYCEDGDMILICGSLYMVGDMRKLIR

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=303, Percent_Identity=27.7227722772277, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI66932990, Length=303, Percent_Identity=27.7227722772277, Blast_Score=138, Evalue=8e-33,
Organism=Escherichia coli, GI1788654, Length=415, Percent_Identity=26.7469879518072, Blast_Score=161, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17553148, Length=443, Percent_Identity=26.1851015801354, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71984923, Length=443, Percent_Identity=26.1851015801354, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17553150, Length=443, Percent_Identity=26.1851015801354, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323760, Length=435, Percent_Identity=32.6436781609195, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6324815, Length=324, Percent_Identity=31.7901234567901, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6322718, Length=440, Percent_Identity=22.5, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24641571, Length=347, Percent_Identity=27.6657060518732, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24581568, Length=172, Percent_Identity=30.8139534883721, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 48889; Mature: 48889

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYKEAREYIQSKAKFGSNLGLERTEKLLELLGNPHKRLRCIHIAGTNGKGSTTAMISAV
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHEEEEEEECCCCCCHHHHHHHHH
LKESGYKVGMYTSPYIEEFEERIQINGHNITKEDLGYIITKVANIVEKVENMGYGNPTEF
HHHCCCEEECCCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
EIITVAMFYYFCLKEVDFAVIEVGLGGRLDSTNVLEPILSIITSISYDHMNILGETLEEI
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TYEKAGIIKKAPVITYPQKKEVEKNIEKVCKEKNCELIKVEDNLINIEIEIIEKNMGQQS
HHHHCCCEECCCEEECCCHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEEEECCCCCCC
FKLKTKEDTYNICLSLLGEHQIKNCITVILALEKLMKLGIKIEKIHIISALKKVKWPARL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEE
EIVNKNPLTVIDGAHNIDGIESLKNNVSKYFKYNKLILILGILKDKQVEDMIKTLVPLAD
EEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHCCCHHHHHHHHHHHHHH
RVLTVAPHNDRGESSKELMHIVLKHNESCEHLEDYKECYDKAKFYCEDGDMILICGSLYM
HEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEECCCCEEEEECCHHH
VGDMRKLIR
HHHHHHHCC
>Mature Secondary Structure
MDYKEAREYIQSKAKFGSNLGLERTEKLLELLGNPHKRLRCIHIAGTNGKGSTTAMISAV
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHEEEEEEECCCCCCHHHHHHHHH
LKESGYKVGMYTSPYIEEFEERIQINGHNITKEDLGYIITKVANIVEKVENMGYGNPTEF
HHHCCCEEECCCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
EIITVAMFYYFCLKEVDFAVIEVGLGGRLDSTNVLEPILSIITSISYDHMNILGETLEEI
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
TYEKAGIIKKAPVITYPQKKEVEKNIEKVCKEKNCELIKVEDNLINIEIEIIEKNMGQQS
HHHHCCCEECCCEEECCCHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEEEECCCCCCC
FKLKTKEDTYNICLSLLGEHQIKNCITVILALEKLMKLGIKIEKIHIISALKKVKWPARL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEE
EIVNKNPLTVIDGAHNIDGIESLKNNVSKYFKYNKLILILGILKDKQVEDMIKTLVPLAD
EEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEHHCCCHHHHHHHHHHHHHH
RVLTVAPHNDRGESSKELMHIVLKHNESCEHLEDYKECYDKAKFYCEDGDMILICGSLYM
HEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEECCCCEEEEECCHHH
VGDMRKLIR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]