| Definition | Clostridium botulinum A str. ATCC 3502, complete genome. |
|---|---|
| Accession | NC_009495 |
| Length | 3,886,916 |
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The map label for this gene is yqiI [H]
Identifier: 148381062
GI number: 148381062
Start: 3304381
End: 3307284
Strand: Reverse
Name: yqiI [H]
Synonym: CBO3111
Alternate gene names: 148381062
Gene position: 3307284-3304381 (Counterclockwise)
Preceding gene: 148381063
Following gene: 148381061
Centisome position: 85.09
GC content: 27.41
Gene sequence:
>2904_bases ATGAGAAATAAAAAATATAATTTAATGCCTAAGATTATTTCATTGTTTTTTATATTATTTTTATTTTCTATAAGAGTACA AGCTATATCAAATATAAGCATTGATTTAAAAACGCCAATACATAAAGGCATAATAAATGATAAGAAATTAAATATTGATG GAGAAATAAAATCTATTTTAAAGTTAAAATCAATATATTTATATATAGATAATAAAAAAATAGGACAAGCCAATTTAAAT GAATTAGAATTTAAAGAAAATACATATAAAACTAGATTAAAATATACAAAAGATATTTCTTCTTTGAAAAATGGAATACA TAATTTAAGAATTTTAGCTATAGATGAACAGAATAACAAAAAAGAAAAAGAAATTAGTATAAATATACAAGAAAATCAAG AAAAATCAGAAACTGATAAAAATATTATACAGAATAATTTAGTAGATACTAATGTAAAGATGGGTAATATAGAAAAAGCA TTAACTACTGTATCTAATGAAGCTAAGCTAGAAAAAGTAGAAGTTTCATACAATGGTAATGTGATAACCAATGGAGAAAT AGGAGTTGGAAAAAGTTACGTTATAAAAGGATATGGAAATTCTGAAAATGGGGTACTGTATCAATTTTGGGTAAAAGATC TATCCACAAATAGTTGGACAATGATAAGAGATTATGGAGAAACAAATAGTTTTAATTACACACCAACAAAGGCTGGAAAG TATCTGATAGGAATACATGTAAAAGATAAGTATAGTAAAGAAAATTTAGATGATTTTATATATGAGAATTATACAGTAAC TGTAAGTAAAGCTAAATTAGAAAAAGTGGAAGTTTCATACGATGGTAATGTAATAACTAATGGAGAAATAGGAGTTGGAA AAAGCTACGTTATAAAAGGATATGGAAATTCTGAAAATGGAGTATTGTATCAATTTTGGGTAAAGGATTTATCTACAAAC AGTTGGACAATGATAAGAGATTATGGAGAAACAAATAGTTTTAATTACACACCAACAAAGGCTGGAAAGTATCTGATAGG AATACATGTAAAAGATAAGTATAGTAAAGAAAATTTAGATGATTTTATATATGAGAATTATACAGTAACTGTAAGTAAAG CTAAATTAGAAAAAGTGGAAGTTTCATACGATGGTAATGTAATAACTAATGGAGAAATAGGAGTTGGAAAAAGCTACGTT ATAAAAGGATATGGAAATTCTGAAAATGGAGTACTGTATCAATTTTGGGTAAAGGATCTATCCACAAATAGTTGGACAAT GATAAGAGATTATGGAGAAACAAATAGTTTTAATTATACACCAGCAAAGGCTGGAAAGTATCTGATAGGAATACATGTAA AAGATAAGTATAGTAAAGAGAATTTAGATGATTTTATATATGAGAATTATGATGTGTCTATAAGTAAAGCTAAATTAGAA AAAGTGGAAGTTTCATACAATGGTAATGTAATAACTGATGGAGAAATAGGAGTTGGAAAAAGCTACGTTATAAAAGGATA TGGAAATTCTGAAAATGGAGTACTGTATCAATTTTGGGTAAAGGATCTATCTACAAATAGTTGGACAATGATAAGAGATT ATGGAGAAACAAATAGTTTTAATTATACACCAGCAAAGGCTGGAAAGTATCTGATAGGAATACATGTAAAAGATAAGTAT AGTAAAGAGAATTTAGATGATTTTATATATGAGAATTATGATGTGTCTATAAGTAAAGCTAAGTTGGAAAAAGTAGAAGT CTCATACAATGGGAATGCAATAACCAATAGTGAAATAGAGACTGGAAAAAATTATACTATAAAAGGATATGGAAATTCTA AAAATGAGATACTGTATCAATTCTGGGTAAAGGATTTATATACAAATAGTTGGACAATGATAAAAGATTATGGAGAAGAG AATAGTATAAATTGGAAACCTACTATTGGTGGTAAATATTTAATAGGAATACATGTAAAGGATAAATATAGTGCTGATAC ATTAGATGATCACATATATGAGAATTATACAATATTGAGTGATAATGCAAGGCTAGAAAAGGTAGAAGTTAAATTAGATG GAAACCTAATAAGTAATAATTTAAATGTAGGAAAGACATATACAATAGAAGGAAGTGCTACATCTAAAAATGGAGTGCTA TATCAATTTTGGGTAAAAGACTTGTCTATAAATAGCTGGGCAATGTTAAGGGATTATGGAGAATCAAATAATATAACATA TACACCAACAAAGAGTGGACAATATTTAATAGGGGTACATGTAAAAGATAAGAATAGCAAGGAAAGCTTAGATGATTTTA AATACAATCAATATAGCATTATAGGTTATAATAAAACAGTAATTGTTGATGCAGGACATGGAGGATATGATAGTGGCGCT GTAGGACCAACCGGTATTAAAGAAAAGGATGTCACTTTAAAAGTAGCTTTAAAGTTAGGAAAAATTTTAGAAAATAGTGG GGTAAATGTTATTTATACTAGAACTAGTGATAATGTATCTTGGCCTTCAAATGAATCACAAGATTTAGCAGCTAGAGTGG CCATTGCAAATAACAATAATACAGATTTATATGTAAGTATACATGCTAATAGTTTTAATGGATCAGCGAATGGAACAGAA ACATATTACTATGATGGGAGTGCAAAAGGAAAAGAAACAGCAGAAAAAGTTCAAAAGGAACTTGTAAATTCTATTGGCTT ATATGATAGAGGCGTTAAAACAGCAGGATATTATGTTTTAAAAAATACTATAAGCCCATCTATATTGGTTGAATTAGGAT TCATAGATAATAGAAATGAAGAAATTCTTTTAAATTCTGATTGGTTTCAACAAAAGTGTGCCGAAGCTATTGCTAAAGGA ATATTATCAACTAGCTTTATGTAA
Upstream 100 bases:
>100_bases AAAAAAAGGTGGTGAAAAGCCATCTTTTTTTATTTTAAAAAGTAAAATTATATACATATATTACGATAATAAAAATATAT AATTTTGAAAGGGTGAGTTT
Downstream 100 bases:
>100_bases CCTAATGAATATAAAAAATAATAAATCAATAAAATAATTATAAAATTGCATATTAAGCTCTAATTATGCTAGTCTTAAAA TAAATAAAAAACCATTAATT
Product: N-acetylmuramoyl-L-alanine amidase
Products: Hydrolyzed cell wall glycopeptides; N-acetylmuramoyl residues; L-amino acid residues [C]
Alternate protein names: NA
Number of amino acids: Translated: 967; Mature: 967
Protein sequence:
>967_residues MRNKKYNLMPKIISLFFILFLFSIRVQAISNISIDLKTPIHKGIINDKKLNIDGEIKSILKLKSIYLYIDNKKIGQANLN ELEFKENTYKTRLKYTKDISSLKNGIHNLRILAIDEQNNKKEKEISINIQENQEKSETDKNIIQNNLVDTNVKMGNIEKA LTTVSNEAKLEKVEVSYNGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGK YLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTN SWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYV IKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLE KVEVSYNGNVITDGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKY SKENLDDFIYENYDVSISKAKLEKVEVSYNGNAITNSEIETGKNYTIKGYGNSKNEILYQFWVKDLYTNSWTMIKDYGEE NSINWKPTIGGKYLIGIHVKDKYSADTLDDHIYENYTILSDNARLEKVEVKLDGNLISNNLNVGKTYTIEGSATSKNGVL YQFWVKDLSINSWAMLRDYGESNNITYTPTKSGQYLIGVHVKDKNSKESLDDFKYNQYSIIGYNKTVIVDAGHGGYDSGA VGPTGIKEKDVTLKVALKLGKILENSGVNVIYTRTSDNVSWPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTE TYYYDGSAKGKETAEKVQKELVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKG ILSTSFM
Sequences:
>Translated_967_residues MRNKKYNLMPKIISLFFILFLFSIRVQAISNISIDLKTPIHKGIINDKKLNIDGEIKSILKLKSIYLYIDNKKIGQANLN ELEFKENTYKTRLKYTKDISSLKNGIHNLRILAIDEQNNKKEKEISINIQENQEKSETDKNIIQNNLVDTNVKMGNIEKA LTTVSNEAKLEKVEVSYNGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGK YLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTN SWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYV IKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLE KVEVSYNGNVITDGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKY SKENLDDFIYENYDVSISKAKLEKVEVSYNGNAITNSEIETGKNYTIKGYGNSKNEILYQFWVKDLYTNSWTMIKDYGEE NSINWKPTIGGKYLIGIHVKDKYSADTLDDHIYENYTILSDNARLEKVEVKLDGNLISNNLNVGKTYTIEGSATSKNGVL YQFWVKDLSINSWAMLRDYGESNNITYTPTKSGQYLIGVHVKDKNSKESLDDFKYNQYSIIGYNKTVIVDAGHGGYDSGA VGPTGIKEKDVTLKVALKLGKILENSGVNVIYTRTSDNVSWPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTE TYYYDGSAKGKETAEKVQKELVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKG ILSTSFM >Mature_967_residues MRNKKYNLMPKIISLFFILFLFSIRVQAISNISIDLKTPIHKGIINDKKLNIDGEIKSILKLKSIYLYIDNKKIGQANLN ELEFKENTYKTRLKYTKDISSLKNGIHNLRILAIDEQNNKKEKEISINIQENQEKSETDKNIIQNNLVDTNVKMGNIEKA LTTVSNEAKLEKVEVSYNGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGK YLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTN SWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYV IKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLE KVEVSYNGNVITDGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKY SKENLDDFIYENYDVSISKAKLEKVEVSYNGNAITNSEIETGKNYTIKGYGNSKNEILYQFWVKDLYTNSWTMIKDYGEE NSINWKPTIGGKYLIGIHVKDKYSADTLDDHIYENYTILSDNARLEKVEVKLDGNLISNNLNVGKTYTIEGSATSKNGVL YQFWVKDLSINSWAMLRDYGESNNITYTPTKSGQYLIGVHVKDKNSKESLDDFKYNQYSIIGYNKTVIVDAGHGGYDSGA VGPTGIKEKDVTLKVALKLGKILENSGVNVIYTRTSDNVSWPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTE TYYYDGSAKGKETAEKVQKELVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKG ILSTSFM
Specific function: Unknown
COG id: COG0860
COG function: function code M; N-acetylmuramoyl-L-alanine amidase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]
Homologues:
Organism=Escherichia coli, GI1788776, Length=228, Percent_Identity=32.0175438596491, Blast_Score=99, Evalue=9e-22, Organism=Escherichia coli, GI87082163, Length=220, Percent_Identity=31.8181818181818, Blast_Score=98, Evalue=3e-21,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002508 [H]
Pfam domain/function: PF01520 Amidase_3 [H]
EC number: 3.5.1.28 [C]
Molecular weight: Translated: 109293; Mature: 109293
Theoretical pI: Translated: 6.93; Mature: 6.93
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 1.0 %Met (Translated Protein) 1.1 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 1.0 %Met (Mature Protein) 1.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRNKKYNLMPKIISLFFILFLFSIRVQAISNISIDLKTPIHKGIINDKKLNIDGEIKSIL CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHCCCCCCEEECCHHHHHHH KLKSIYLYIDNKKIGQANLNELEFKENTYKTRLKYTKDISSLKNGIHNLRILAIDEQNNK EEEEEEEEECCCCCCCCCCCCEEECCCCEEEHEEHHHHHHHHHCCCCEEEEEEEECCCCC KEKEISINIQENQEKSETDKNIIQNNLVDTNVKMGNIEKALTTVSNEAKLEKVEVSYNGN CCEEEEEEEECCCCHHHHHHHHHHCCCEECCEECCCHHHHHHHCCCCCEEEEEEEEECCC VITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGK EEECCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCC YLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYVIKG EEEEEEEECCCCCCCCHHHHHCCEEEEEEECEEEEEEEEECCCEEECCCCCCCCEEEEEE YGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLD CCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCH DFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDL HHHHCCEEEEEEECEEEEEEEEECCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECC STNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLE CCCCEEEEEECCCCCCCCCCHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCEEEEEEEEE KVEVSYNGNVITDGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSF EEEEEECCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCC NYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLEKVEVSYNGNAITNSEIE CCCHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCEEEEEEEEEEEEEEECCCEEECCCCC TGKNYTIKGYGNSKNEILYQFWVKDLYTNSWTMIKDYGEENSINWKPTIGGKYLIGIHVK CCCCEEEEECCCCCCCEEEEEEHHHHHCCCEEEEEECCCCCCCEEEECCCCEEEEEEEEE DKYSADTLDDHIYENYTILSDNARLEKVEVKLDGNLISNNLNVGKTYTIEGSATSKNGVL CCCCCCCHHHHHHCCEEEEECCCEEEEEEEEECCEEEECCCCCCEEEEEECCCCCCCCEE YQFWVKDLSINSWAMLRDYGESNNITYTPTKSGQYLIGVHVKDKNSKESLDDFKYNQYSI EEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEECCCCCCCHHHCCCCCEEE IGYNKTVIVDAGHGGYDSGAVGPTGIKEKDVTLKVALKLGKILENSGVNVIYTRTSDNVS EECCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCCEEEEEECCCCCC WPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTETYYYDGSAKGKETAEKVQKE CCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHH LVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKG HHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCEEEECCHHHHHHHHHHHHHH ILSTSFM HHHHCCC >Mature Secondary Structure MRNKKYNLMPKIISLFFILFLFSIRVQAISNISIDLKTPIHKGIINDKKLNIDGEIKSIL CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHCCCCCCEEECCHHHHHHH KLKSIYLYIDNKKIGQANLNELEFKENTYKTRLKYTKDISSLKNGIHNLRILAIDEQNNK EEEEEEEEECCCCCCCCCCCCEEECCCCEEEHEEHHHHHHHHHCCCCEEEEEEEECCCCC KEKEISINIQENQEKSETDKNIIQNNLVDTNVKMGNIEKALTTVSNEAKLEKVEVSYNGN CCEEEEEEEECCCCHHHHHHHHHHCCCEECCEECCCHHHHHHHCCCCCEEEEEEEEECCC VITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGK EEECCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCC YLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYVIKG EEEEEEEECCCCCCCCHHHHHCCEEEEEEECEEEEEEEEECCCEEECCCCCCCCEEEEEE YGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLD CCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCH DFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDL HHHHCCEEEEEEECEEEEEEEEECCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECC STNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLE CCCCEEEEEECCCCCCCCCCHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCEEEEEEEEE KVEVSYNGNVITDGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSF EEEEEECCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCC NYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLEKVEVSYNGNAITNSEIE CCCHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCEEEEEEEEEEEEEEECCCEEECCCCC TGKNYTIKGYGNSKNEILYQFWVKDLYTNSWTMIKDYGEENSINWKPTIGGKYLIGIHVK CCCCEEEEECCCCCCCEEEEEEHHHHHCCCEEEEEECCCCCCCEEEECCCCEEEEEEEEE DKYSADTLDDHIYENYTILSDNARLEKVEVKLDGNLISNNLNVGKTYTIEGSATSKNGVL CCCCCCCHHHHHHCCEEEEECCCEEEEEEEEECCEEEECCCCCCEEEEEECCCCCCCCEE YQFWVKDLSINSWAMLRDYGESNNITYTPTKSGQYLIGVHVKDKNSKESLDDFKYNQYSI EEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEECCCCCCCHHHCCCCCEEE IGYNKTVIVDAGHGGYDSGAVGPTGIKEKDVTLKVALKLGKILENSGVNVIYTRTSDNVS EECCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCCEEEEEECCCCCC WPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTETYYYDGSAKGKETAEKVQKE CCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHH LVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKG HHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCEEEECCHHHHHHHHHHHHHH ILSTSFM HHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: cell wall glycopeptides; D-lactyl-L-Ala [C]
Specific reaction: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues [C]
General reaction: Carboxylic acid amide hydrolysis [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8969508; 9384377 [H]