| Definition | Clostridium botulinum A str. ATCC 3502, complete genome. |
|---|---|
| Accession | NC_009495 |
| Length | 3,886,916 |
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The map label for this gene is amiC [H]
Identifier: 148381061
GI number: 148381061
Start: 3301295
End: 3304144
Strand: Reverse
Name: amiC [H]
Synonym: CBO3110
Alternate gene names: 148381061
Gene position: 3304144-3301295 (Counterclockwise)
Preceding gene: 148381062
Following gene: 148381060
Centisome position: 85.01
GC content: 29.75
Gene sequence:
>2850_bases ATGTATATGGGGGAAATAGAAAATATAGTTAAAAAGATTCCATTAATACTTTTAATTAGTATTATTGTAACTTTTGTACC TTCATTTAAAGTACATGCTAGTAGCACTACACATATAATGGGACAACCTCAGTTAACACAGGAACAAGCATTAAATTATT TTAAGACCAGAAACAGTGAAAAAAGTGACCAAGCTACAAAAGAATTTATTTCCATAGTTTGGCAGGAAGCTAATTTAGAA GGAATAAGAGCTGATGTAGTTTTTATTCAAATAATGAAGGAAACAAATTTTTTGAAATTTACAGGGGATGTAAAAGAATG TCAAAATAATTTTGCAGGTATTGGTGCTACAGGTGGTGGTGTACCAGGTGCTTATTTTAAAGATACAAAAACAGGAGTAA GAGCCGTTGTTCAACATCTAAAAGCTTATTGTAGTACTGAGGGATTAAAAAATCCTTGTGTAGATCCACGATTTACATAT GTTCAAAGAGGAATAAGTCCCTATGTTGAGTGGTTAGGTATAGGGGAAAATCCTAATTATCCAGATAAAGGATGGGCTGC GGATAATAATTATGGTAAGAGTATAGTTGAAATGATGCACAGTGCAAAGTATCTGGCAAATGAAGGTGATTCGCAAGGAA ATATAACTACTTCAAAGGCTACTATTAATAATTTAGAAGTAAGTTTAGATGGAAATAGTGTAGTAACTAATGAATTAGAA CCGGGAAAAGCATATAACATAAAAGCTTATGGGAATAGTTCTAATGGAGTACTATATGAATATTGGATAAAAGACTTATC AATTAATTCATGGATAAAGCTAAGAGATTATAGTACAACCAATGAAGTTAAATGGACACCAAATAAATCAGGGAAATATC TAATAGGAGTACATGTTAAAGATAGATATAGTAAGGAAAGATTAGATAATTTTAAGTATGTAGAATATAATGTAGCATCC CTTAAAAAAGCTACTATTAGTAGTTTAGAAGTAAGCCTAGATGGAAATAATGTAGTGAATAATGAATTACAACTAGGCAA GATATATAATATAAAAGCTTATGGCAGTAGCTCTAATGGAGTATTGTATGAATATTGGATAAAAGACTTATCAAAGAATT CATGGATGAAGTTAAGAGATTATAGTACAAGTACTCAAGTCACATGGACACCAAATAAATCAGGAAAGTATCTACTAGGG GTACATGTTAAAGATAGGTATAGTGAAGAAAGATTAGATAACTTTAAGTATGTAGAATACAATGTAGCATCACCTAAAAA GGCTACTGTTGATAGCTTAGAAGTAAGCTTAGATGGAAATAAGGTAGAAACTAATGAATTAGATCCAGGAAAAGCATATA ACATAAAAGCTTATGGGAATAGTTCTAATGGTGTATTATATGAATATTGGATAAAAGACTTATCGATTAATTCATGGATA AAGTTAAAAGATTATAGTACAAGTACTCAAGTTACATGGACACCGAATAAGTCAGGAAAATATTTAATAGGAGTGCACGT AAAAGATAAATATAGTACACAAAAATTAGATAATTTTAAATATGTAGAATATAATGTAGCATCACCTAAAAAAGCTACTG TCAATAGTTTAGAAGTAAGTCTAAATGAAGGCAAAGTAGTAAATAATGAACTACAAGCAGGTGAAATATATAATATAAAA GCTTATGGTAGTAGTTCTAATGGAGTATTATATGAATACTGGATAAAAGACCTATCAATTAATTCATGGATAAAGTTAAA AGATTATAGTACAAGTACTCAAGTTACATGGACACCAAATAAGTCAGGAAAATATCTGATAGGGGTACACGTAAAAGATA AATATAGTGCACAGAAATTAGATAATTTTAAGTATGTAGAATATAATGTGAAACTATCTAAAAAAGCAGTTATTAGTAAC TTGGAAGTAAGCCTAAATGGAAATATAGTAGCAAACAATCAATTAAATTCAGGAAAAACATATAGTATAAAAGCTTATGC CGAAAGTCTTAATGGAGTATTGTATGAATATTGGATAAAAGATTTATCAAGTAATTCATGGATAAAGTTAAAAGATTATA GTACAAGTGCTCAAATCACATGGACACCAAATAAAGCAGGAAAATATCTAATAGGAGTACATGTAAAAGATAAATATAGT AATGAAAGATTAGACAATTATAAATATGTAGAATATAGTGTTCAAGGTGGTCTAATAAAAACTATAGTTCTAGATGCAGG TCATGGAGGCAAGGACTCAGGTGCAGTATCTTCTAGGGCAACTGGAAATATACACGAAGCAGATATAGTTCAAAAAATTA CCATCAAGTTGGGAAACCTGCTAAAAAAAGCAGGATATAATGTTATTTATACTAGAGATAAGGTAGATAATTATAATTAT ACCTCAGTAACACAAAATTTAGAAGATAGAATAAATGTGGCTAATAGTATAAATGCTGATTTGTTTATGAGTATACATGC TGATTCTTTTGATATACCATCAGCTAATGGATATAGCGCTCATTATAGTAGTTATAGACCTAAGCTAGATAATTCAGGTG TATATATAGAGGATGATGTGTATTATGATAGAACTCCTTGTGATGCAGCATTAAAGAGTAAAGTTTTATCACAACTTATA GTAAATGAGATGTCTTCTTTAGGAGGATCAAATAGAGGAATAAGTGATCATAATTTATATGTTACAAAAAATGCTCTAAT GCCTTCTGTACTTGTAGAATGTGGATTTGTTTCTAATGATGCAGAAGTTAGAAAGTTAAATGGTGACACTTATCAAAATC AAATAGCTCAAAAACTTTACAATGCAGTAACAAAATTGTTTAGTATGTAG
Upstream 100 bases:
>100_bases TATAAATAAAATTATAAATAAAACATATGATATAATAATTTACAAAGGAGGTGAATTTATGAATGGAAAAAAATTTTTAC TAAAAGAAAGGAGGGGAAAT
Downstream 100 bases:
>100_bases TTTAGAAAACCGTTGATATATTTCTTATCAACGGTTTTATTAATTTATTTATAACTTATAAATGATATTTTCTATTTATT GTATATATGATAAAATAAAG
Product: N-acetylmuramoyl-L-alanine amidase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 949; Mature: 949
Protein sequence:
>949_residues MYMGEIENIVKKIPLILLISIIVTFVPSFKVHASSTTHIMGQPQLTQEQALNYFKTRNSEKSDQATKEFISIVWQEANLE GIRADVVFIQIMKETNFLKFTGDVKECQNNFAGIGATGGGVPGAYFKDTKTGVRAVVQHLKAYCSTEGLKNPCVDPRFTY VQRGISPYVEWLGIGENPNYPDKGWAADNNYGKSIVEMMHSAKYLANEGDSQGNITTSKATINNLEVSLDGNSVVTNELE PGKAYNIKAYGNSSNGVLYEYWIKDLSINSWIKLRDYSTTNEVKWTPNKSGKYLIGVHVKDRYSKERLDNFKYVEYNVAS LKKATISSLEVSLDGNNVVNNELQLGKIYNIKAYGSSSNGVLYEYWIKDLSKNSWMKLRDYSTSTQVTWTPNKSGKYLLG VHVKDRYSEERLDNFKYVEYNVASPKKATVDSLEVSLDGNKVETNELDPGKAYNIKAYGNSSNGVLYEYWIKDLSINSWI KLKDYSTSTQVTWTPNKSGKYLIGVHVKDKYSTQKLDNFKYVEYNVASPKKATVNSLEVSLNEGKVVNNELQAGEIYNIK AYGSSSNGVLYEYWIKDLSINSWIKLKDYSTSTQVTWTPNKSGKYLIGVHVKDKYSAQKLDNFKYVEYNVKLSKKAVISN LEVSLNGNIVANNQLNSGKTYSIKAYAESLNGVLYEYWIKDLSSNSWIKLKDYSTSAQITWTPNKAGKYLIGVHVKDKYS NERLDNYKYVEYSVQGGLIKTIVLDAGHGGKDSGAVSSRATGNIHEADIVQKITIKLGNLLKKAGYNVIYTRDKVDNYNY TSVTQNLEDRINVANSINADLFMSIHADSFDIPSANGYSAHYSSYRPKLDNSGVYIEDDVYYDRTPCDAALKSKVLSQLI VNEMSSLGGSNRGISDHNLYVTKNALMPSVLVECGFVSNDAEVRKLNGDTYQNQIAQKLYNAVTKLFSM
Sequences:
>Translated_949_residues MYMGEIENIVKKIPLILLISIIVTFVPSFKVHASSTTHIMGQPQLTQEQALNYFKTRNSEKSDQATKEFISIVWQEANLE GIRADVVFIQIMKETNFLKFTGDVKECQNNFAGIGATGGGVPGAYFKDTKTGVRAVVQHLKAYCSTEGLKNPCVDPRFTY VQRGISPYVEWLGIGENPNYPDKGWAADNNYGKSIVEMMHSAKYLANEGDSQGNITTSKATINNLEVSLDGNSVVTNELE PGKAYNIKAYGNSSNGVLYEYWIKDLSINSWIKLRDYSTTNEVKWTPNKSGKYLIGVHVKDRYSKERLDNFKYVEYNVAS LKKATISSLEVSLDGNNVVNNELQLGKIYNIKAYGSSSNGVLYEYWIKDLSKNSWMKLRDYSTSTQVTWTPNKSGKYLLG VHVKDRYSEERLDNFKYVEYNVASPKKATVDSLEVSLDGNKVETNELDPGKAYNIKAYGNSSNGVLYEYWIKDLSINSWI KLKDYSTSTQVTWTPNKSGKYLIGVHVKDKYSTQKLDNFKYVEYNVASPKKATVNSLEVSLNEGKVVNNELQAGEIYNIK AYGSSSNGVLYEYWIKDLSINSWIKLKDYSTSTQVTWTPNKSGKYLIGVHVKDKYSAQKLDNFKYVEYNVKLSKKAVISN LEVSLNGNIVANNQLNSGKTYSIKAYAESLNGVLYEYWIKDLSSNSWIKLKDYSTSAQITWTPNKAGKYLIGVHVKDKYS NERLDNYKYVEYSVQGGLIKTIVLDAGHGGKDSGAVSSRATGNIHEADIVQKITIKLGNLLKKAGYNVIYTRDKVDNYNY TSVTQNLEDRINVANSINADLFMSIHADSFDIPSANGYSAHYSSYRPKLDNSGVYIEDDVYYDRTPCDAALKSKVLSQLI VNEMSSLGGSNRGISDHNLYVTKNALMPSVLVECGFVSNDAEVRKLNGDTYQNQIAQKLYNAVTKLFSM >Mature_949_residues MYMGEIENIVKKIPLILLISIIVTFVPSFKVHASSTTHIMGQPQLTQEQALNYFKTRNSEKSDQATKEFISIVWQEANLE GIRADVVFIQIMKETNFLKFTGDVKECQNNFAGIGATGGGVPGAYFKDTKTGVRAVVQHLKAYCSTEGLKNPCVDPRFTY VQRGISPYVEWLGIGENPNYPDKGWAADNNYGKSIVEMMHSAKYLANEGDSQGNITTSKATINNLEVSLDGNSVVTNELE PGKAYNIKAYGNSSNGVLYEYWIKDLSINSWIKLRDYSTTNEVKWTPNKSGKYLIGVHVKDRYSKERLDNFKYVEYNVAS LKKATISSLEVSLDGNNVVNNELQLGKIYNIKAYGSSSNGVLYEYWIKDLSKNSWMKLRDYSTSTQVTWTPNKSGKYLLG VHVKDRYSEERLDNFKYVEYNVASPKKATVDSLEVSLDGNKVETNELDPGKAYNIKAYGNSSNGVLYEYWIKDLSINSWI KLKDYSTSTQVTWTPNKSGKYLIGVHVKDKYSTQKLDNFKYVEYNVASPKKATVNSLEVSLNEGKVVNNELQAGEIYNIK AYGSSSNGVLYEYWIKDLSINSWIKLKDYSTSTQVTWTPNKSGKYLIGVHVKDKYSAQKLDNFKYVEYNVKLSKKAVISN LEVSLNGNIVANNQLNSGKTYSIKAYAESLNGVLYEYWIKDLSSNSWIKLKDYSTSAQITWTPNKAGKYLIGVHVKDKYS NERLDNYKYVEYSVQGGLIKTIVLDAGHGGKDSGAVSSRATGNIHEADIVQKITIKLGNLLKKAGYNVIYTRDKVDNYNY TSVTQNLEDRINVANSINADLFMSIHADSFDIPSANGYSAHYSSYRPKLDNSGVYIEDDVYYDRTPCDAALKSKVLSQLI VNEMSSLGGSNRGISDHNLYVTKNALMPSVLVECGFVSNDAEVRKLNGDTYQNQIAQKLYNAVTKLFSM
Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Periplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]
Homologues:
Organism=Escherichia coli, GI87082163, Length=229, Percent_Identity=30.5676855895196, Blast_Score=85, Evalue=2e-17, Organism=Escherichia coli, GI1788776, Length=235, Percent_Identity=27.6595744680851, Blast_Score=75, Evalue=2e-14,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR021731 - InterPro: IPR002508 [H]
Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]
EC number: =3.5.1.28 [H]
Molecular weight: Translated: 106583; Mature: 106583
Theoretical pI: Translated: 9.06; Mature: 9.06
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 1.7 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 1.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MYMGEIENIVKKIPLILLISIIVTFVPSFKVHASSTTHIMGQPQLTQEQALNYFKTRNSE CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCHHHHHHHHHHCCCC KSDQATKEFISIVWQEANLEGIRADVVFIQIMKETNFLKFTGDVKECQNNFAGIGATGGG CHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEEECCHHHHHCCCCCCCCCCCC VPGAYFKDTKTGVRAVVQHLKAYCSTEGLKNPCVDPRFTYVQRGISPYVEWLGIGENPNY CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHHEEECCCCCCC PDKGWAADNNYGKSIVEMMHSAKYLANEGDSQGNITTSKATINNLEVSLDGNSVVTNELE CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECEEEEEEEEEEECCCEEEECCCC PGKAYNIKAYGNSSNGVLYEYWIKDLSINSWIKLRDYSTTNEVKWTPNKSGKYLIGVHVK CCCEEEEEEECCCCCCEEEEEEEECCCCCCEEEEEECCCCCCEEECCCCCCCEEEEEEEC DRYSKERLDNFKYVEYNVASLKKATISSLEVSLDGNNVVNNELQLGKIYNIKAYGSSSNG CHHCHHHHCCCEEEEEEHHHHHHHEEEEEEEEECCCCEECCCEEECEEEEEEEECCCCCC VLYEYWIKDLSKNSWMKLRDYSTSTQVTWTPNKSGKYLLGVHVKDRYSEERLDNFKYVEY EEEEEEHHHCCCCCCEEEEECCCCCEEEECCCCCCCEEEEEEEHHCCCHHHHCCEEEEEE NVASPKKATVDSLEVSLDGNKVETNELDPGKAYNIKAYGNSSNGVLYEYWIKDLSINSWI ECCCCCCCCCCEEEEEECCCEEECCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCEE KLKDYSTSTQVTWTPNKSGKYLIGVHVKDKYSTQKLDNFKYVEYNVASPKKATVNSLEVS EEEECCCCEEEEECCCCCCCEEEEEEEECCCCHHHCCCEEEEEEECCCCCCCEEEEEEEE LNEGKVVNNELQAGEIYNIKAYGSSSNGVLYEYWIKDLSINSWIKLKDYSTSTQVTWTPN ECCCEEECCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCEEEEEECCCCEEEEECCC KSGKYLIGVHVKDKYSAQKLDNFKYVEYNVKLSKKAVISNLEVSLNGNIVANNQLNSGKT CCCCEEEEEEECCCCCHHHCCCEEEEEEEEEECCHHHEEEEEEEECCCEEECCCCCCCCE YSIKAYAESLNGVLYEYWIKDLSSNSWIKLKDYSTSAQITWTPNKAGKYLIGVHVKDKYS EEEEEHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCEEEEEEEECCCC NERLDNYKYVEYSVQGGLIKTIVLDAGHGGKDSGAVSSRATGNIHEADIVQKITIKLGNL CCCCCCEEEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH LKKAGYNVIYTRDKVDNYNYTSVTQNLEDRINVANSINADLFMSIHADSFDIPSANGYSA HHHCCCEEEEEECCCCCCCCHHHHHCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCC HYSSYRPKLDNSGVYIEDDVYYDRTPCDAALKSKVLSQLIVNEMSSLGGSNRGISDHNLY HHCCCCCCCCCCCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE VTKNALMPSVLVECGFVSNDAEVRKLNGDTYQNQIAQKLYNAVTKLFSM EECCCCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCC >Mature Secondary Structure MYMGEIENIVKKIPLILLISIIVTFVPSFKVHASSTTHIMGQPQLTQEQALNYFKTRNSE CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCHHHHHHHHHHCCCC KSDQATKEFISIVWQEANLEGIRADVVFIQIMKETNFLKFTGDVKECQNNFAGIGATGGG CHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEEECCHHHHHCCCCCCCCCCCC VPGAYFKDTKTGVRAVVQHLKAYCSTEGLKNPCVDPRFTYVQRGISPYVEWLGIGENPNY CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHHEEECCCCCCC PDKGWAADNNYGKSIVEMMHSAKYLANEGDSQGNITTSKATINNLEVSLDGNSVVTNELE CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECEEEEEEEEEEECCCEEEECCCC PGKAYNIKAYGNSSNGVLYEYWIKDLSINSWIKLRDYSTTNEVKWTPNKSGKYLIGVHVK CCCEEEEEEECCCCCCEEEEEEEECCCCCCEEEEEECCCCCCEEECCCCCCCEEEEEEEC DRYSKERLDNFKYVEYNVASLKKATISSLEVSLDGNNVVNNELQLGKIYNIKAYGSSSNG CHHCHHHHCCCEEEEEEHHHHHHHEEEEEEEEECCCCEECCCEEECEEEEEEEECCCCCC VLYEYWIKDLSKNSWMKLRDYSTSTQVTWTPNKSGKYLLGVHVKDRYSEERLDNFKYVEY EEEEEEHHHCCCCCCEEEEECCCCCEEEECCCCCCCEEEEEEEHHCCCHHHHCCEEEEEE NVASPKKATVDSLEVSLDGNKVETNELDPGKAYNIKAYGNSSNGVLYEYWIKDLSINSWI ECCCCCCCCCCEEEEEECCCEEECCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCEE KLKDYSTSTQVTWTPNKSGKYLIGVHVKDKYSTQKLDNFKYVEYNVASPKKATVNSLEVS EEEECCCCEEEEECCCCCCCEEEEEEEECCCCHHHCCCEEEEEEECCCCCCCEEEEEEEE LNEGKVVNNELQAGEIYNIKAYGSSSNGVLYEYWIKDLSINSWIKLKDYSTSTQVTWTPN ECCCEEECCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCEEEEEECCCCEEEEECCC KSGKYLIGVHVKDKYSAQKLDNFKYVEYNVKLSKKAVISNLEVSLNGNIVANNQLNSGKT CCCCEEEEEEECCCCCHHHCCCEEEEEEEEEECCHHHEEEEEEEECCCEEECCCCCCCCE YSIKAYAESLNGVLYEYWIKDLSSNSWIKLKDYSTSAQITWTPNKAGKYLIGVHVKDKYS EEEEEHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCEEEEEEEECCCC NERLDNYKYVEYSVQGGLIKTIVLDAGHGGKDSGAVSSRATGNIHEADIVQKITIKLGNL CCCCCCEEEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH LKKAGYNVIYTRDKVDNYNYTSVTQNLEDRINVANSINADLFMSIHADSFDIPSANGYSA HHHCCCEEEEEECCCCCCCCHHHHHCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCC HYSSYRPKLDNSGVYIEDDVYYDRTPCDAALKSKVLSQLIVNEMSSLGGSNRGISDHNLY HHCCCCCCCCCCCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE VTKNALMPSVLVECGFVSNDAEVRKLNGDTYQNQIAQKLYNAVTKLFSM EECCCCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10710307 [H]