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The map label for this gene is epsC [H]

Identifier: 148381060

GI number: 148381060

Start: 3299249

End: 3301114

Strand: Reverse

Name: epsC [H]

Synonym: CBO3109

Alternate gene names: 148381060

Gene position: 3301114-3299249 (Counterclockwise)

Preceding gene: 148381061

Following gene: 148381059

Centisome position: 84.93

GC content: 26.85

Gene sequence:

>1866_bases
ATGAGAAAAGATAGAGAATTGATTCTTTATGATATATTATTAATAATTTTATCATTATATTTAGCAATTTTATTAAGATT
CGATTTCGGTATGGCTTCTATGGCTTCGGAATTTAAAAAATATATGATATTTTTTAAGTTATCAGTAATTCCAGTTATAA
TAATAATCTTATTTTTTAATAAAATATTTAATTTATATAGTAGTATATGGAAATATGCTTCAGTAGAAGAACTTATGTCT
ATAATTTATTCAGTTTCTATATCTAATATTATATTCATAATTTATAGTTATTTTATTAATTATAAAGTTCTAGAAAGTAA
TTATTATAGATTCCCATTTACTGTACATATAATATTTTGGATTCTATCTATAGTATCCCTAGGAGGTACAAGATTTATAT
ATAGAGTTATAGAAGATAAGGAATGTAATAAGAACAAATGTAACTGTGATTCTAAAGAAAAGAAGTTACTTATAATAGGG
GCAGGGGATGCAGCAGCTCTCATTATAAAAGAAATAAAAAGAAATAAGGAATTAAATTATAGTATAATAGGTCTTGTAGA
TGATGATATAGAGAAGTTAGGAAAGAGAATAAATGGTATACCTGTTTTAGGGGGAAGAGAAAGTATTATAAAGATATCTA
AAAATAAAAAAATAGATGAAATAATATTAGCTATACCTTCAGCAGCATCTTCAACAAAAAGTGAAATAGTATCTATATGT
AAAGAAACTAGTTGTAAATTAAAAACTTTACCAAGCATGGATAAGGTAATACAGGGTAAATTTAATATGAGCAAGTTAAG
AGATGTAAGTATTGAGGATTTGCTTGGGAGAGAGGAAGTTAAACTAGATGATAGTGGAATAAATAGATATATAAAGGATA
AAGTAGTTTTAGTTACTGGTGGAGGAGGGTCTATAGGTTCAGAACTTTGTAGACAAATAGTTAAATTTTCACCTAAAAAA
CTTTTAATATTAGATATATATGAAAACAATGCATATGATGTCCAAATGGAACTAAATTATAAATATCCAGAACTGGACAA
AGCTGTTATAATAGCTTCTATAAGGGATGAAAAGAGGCTAAGAAATATATTTTCATTATATAAACCAGATGTAGTTTTTC
ATGCAGCAGCCCATAAGCATGTTCCACTAATGGAAGAGAATCCAGCAGAATCTATTAAAAATAACGTAATAGGTACCTAT
AATCTAATAAAATGTGCCCATGAATTTGGGATTGAGCGTTTTGTACAAATAAGTACAGATAAAGCTGTTAATCCAACTAA
TATAATGGGAGCTACAAAAAGATTTTGTGAAATAATGATTCAAGCTTTTGATAAAATAAGTAAAACTGAATTTGTAGCTG
TTAGATTTGGGAATGTTTTAGGAAGTAATGGATCTGTTATTCCATTATTTAAAAAACAAATATCTCATGGAGGACCTGTC
ACTGTAACTCATCCAGAAATAAATAGATATTTTATGACTATACCAGAAGCAGCTCAATTAGTTATACAAGCAGGAGCTAT
GGCAAAAGGTGGAGAAATATTTGTTCTGGATATGGGAAAACCAGTTAAAATTGTAGATTTAGCTAAAGATTTAATAACTT
TATCTGGATATAAGCCAGAGGAAGATATTAAGATTGAATACACAGGATTAAGACCTGGGGAAAAATTATATGAAGAACTA
TTAATGGATGAAATAGCTTTAACATCAACAGGACATAATAAAATATTTGTAGAAAAACCTATGGAAAATAATATAGACTT
TGTAGAAAAATCCATAGAAGAATTTAAAAAAGTTGTTTATAATGATGAAGAAGAAATATTTGCATTGATAGAAAAGAAGG
TTCCAACATATATAAGAAAAAAATAA

Upstream 100 bases:

>100_bases
GTATATATGATAAAATAAAGATATTGCAAAAAATTGTTGAATAATTTTATCAATGATGTAAAATAAATAAGTAGAGTTTA
GAGTTTAAGGAAGTGATTAT

Downstream 100 bases:

>100_bases
TGCTTAACAAATAATAGGTAAGTTTTATTTGCAAAATTTAAATATAAAATAATAGTAGCTATAGAATAGTGACAAAAAAT
TAAAAATATATAAATTAAGC

Product: exopolysaccharide biosynthesis protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MRKDRELILYDILLIILSLYLAILLRFDFGMASMASEFKKYMIFFKLSVIPVIIIILFFNKIFNLYSSIWKYASVEELMS
IIYSVSISNIIFIIYSYFINYKVLESNYYRFPFTVHIIFWILSIVSLGGTRFIYRVIEDKECNKNKCNCDSKEKKLLIIG
AGDAAALIIKEIKRNKELNYSIIGLVDDDIEKLGKRINGIPVLGGRESIIKISKNKKIDEIILAIPSAASSTKSEIVSIC
KETSCKLKTLPSMDKVIQGKFNMSKLRDVSIEDLLGREEVKLDDSGINRYIKDKVVLVTGGGGSIGSELCRQIVKFSPKK
LLILDIYENNAYDVQMELNYKYPELDKAVIIASIRDEKRLRNIFSLYKPDVVFHAAAHKHVPLMEENPAESIKNNVIGTY
NLIKCAHEFGIERFVQISTDKAVNPTNIMGATKRFCEIMIQAFDKISKTEFVAVRFGNVLGSNGSVIPLFKKQISHGGPV
TVTHPEINRYFMTIPEAAQLVIQAGAMAKGGEIFVLDMGKPVKIVDLAKDLITLSGYKPEEDIKIEYTGLRPGEKLYEEL
LMDEIALTSTGHNKIFVEKPMENNIDFVEKSIEEFKKVVYNDEEEIFALIEKKVPTYIRKK

Sequences:

>Translated_621_residues
MRKDRELILYDILLIILSLYLAILLRFDFGMASMASEFKKYMIFFKLSVIPVIIIILFFNKIFNLYSSIWKYASVEELMS
IIYSVSISNIIFIIYSYFINYKVLESNYYRFPFTVHIIFWILSIVSLGGTRFIYRVIEDKECNKNKCNCDSKEKKLLIIG
AGDAAALIIKEIKRNKELNYSIIGLVDDDIEKLGKRINGIPVLGGRESIIKISKNKKIDEIILAIPSAASSTKSEIVSIC
KETSCKLKTLPSMDKVIQGKFNMSKLRDVSIEDLLGREEVKLDDSGINRYIKDKVVLVTGGGGSIGSELCRQIVKFSPKK
LLILDIYENNAYDVQMELNYKYPELDKAVIIASIRDEKRLRNIFSLYKPDVVFHAAAHKHVPLMEENPAESIKNNVIGTY
NLIKCAHEFGIERFVQISTDKAVNPTNIMGATKRFCEIMIQAFDKISKTEFVAVRFGNVLGSNGSVIPLFKKQISHGGPV
TVTHPEINRYFMTIPEAAQLVIQAGAMAKGGEIFVLDMGKPVKIVDLAKDLITLSGYKPEEDIKIEYTGLRPGEKLYEEL
LMDEIALTSTGHNKIFVEKPMENNIDFVEKSIEEFKKVVYNDEEEIFALIEKKVPTYIRKK
>Mature_621_residues
MRKDRELILYDILLIILSLYLAILLRFDFGMASMASEFKKYMIFFKLSVIPVIIIILFFNKIFNLYSSIWKYASVEELMS
IIYSVSISNIIFIIYSYFINYKVLESNYYRFPFTVHIIFWILSIVSLGGTRFIYRVIEDKECNKNKCNCDSKEKKLLIIG
AGDAAALIIKEIKRNKELNYSIIGLVDDDIEKLGKRINGIPVLGGRESIIKISKNKKIDEIILAIPSAASSTKSEIVSIC
KETSCKLKTLPSMDKVIQGKFNMSKLRDVSIEDLLGREEVKLDDSGINRYIKDKVVLVTGGGGSIGSELCRQIVKFSPKK
LLILDIYENNAYDVQMELNYKYPELDKAVIIASIRDEKRLRNIFSLYKPDVVFHAAAHKHVPLMEENPAESIKNNVIGTY
NLIKCAHEFGIERFVQISTDKAVNPTNIMGATKRFCEIMIQAFDKISKTEFVAVRFGNVLGSNGSVIPLFKKQISHGGPV
TVTHPEINRYFMTIPEAAQLVIQAGAMAKGGEIFVLDMGKPVKIVDLAKDLITLSGYKPEEDIKIEYTGLRPGEKLYEEL
LMDEIALTSTGHNKIFVEKPMENNIDFVEKSIEEFKKVVYNDEEEIFALIEKKVPTYIRKK

Specific function: Involved in biofilm formation [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=286, Percent_Identity=27.972027972028, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI48994969, Length=140, Percent_Identity=30.7142857142857, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 70630; Mature: 70630

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKDRELILYDILLIILSLYLAILLRFDFGMASMASEFKKYMIFFKLSVIPVIIIILFFN
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIFNLYSSIWKYASVEELMSIIYSVSISNIIFIIYSYFINYKVLESNYYRFPFTVHIIFW
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEECCHHHHHHHH
ILSIVSLGGTRFIYRVIEDKECNKNKCNCDSKEKKLLIIGAGDAAALIIKEIKRNKELNY
HHHHHHCCCHHEEHEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCE
SIIGLVDDDIEKLGKRINGIPVLGGRESIIKISKNKKIDEIILAIPSAASSTKSEIVSIC
EEEEEECHHHHHHHHHHCCCEEECCCCCEEEECCCCCCCHHEEECCCCCCHHHHHHHHHH
KETSCKLKTLPSMDKVIQGKFNMSKLRDVSIEDLLGREEVKLDDSGINRYIKDKVVLVTG
HHCCCCEECCCCHHHHHCCCCCHHHHCCCCHHHHCCCCCEEECHHHCCHHCCCEEEEEEC
GGGSIGSELCRQIVKFSPKKLLILDIYENNAYDVQMELNYKYPELDKAVIIASIRDEKRL
CCCCHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCEEEEEECHHHHHH
RNIFSLYKPDVVFHAAAHKHVPLMEENPAESIKNNVIGTYNLIKCAHEFGIERFVQISTD
HHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCHHHEEEECCC
KAVNPTNIMGATKRFCEIMIQAFDKISKTEFVAVRFGNVLGSNGSVIPLFKKQISHGGPV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCCCCCEEHHHHHHHCCCCCE
TVTHPEINRYFMTIPEAAQLVIQAGAMAKGGEIFVLDMGKPVKIVDLAKDLITLSGYKPE
EEECCCCCCEEEECCHHHHHHHHHCCCCCCCEEEEEECCCCEEHHHHHHHHHHHCCCCCC
EDIKIEYTGLRPGEKLYEELLMDEIALTSTGHNKIFVEKPMENNIDFVEKSIEEFKKVVY
CCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHC
NDEEEIFALIEKKVPTYIRKK
CCHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MRKDRELILYDILLIILSLYLAILLRFDFGMASMASEFKKYMIFFKLSVIPVIIIILFFN
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIFNLYSSIWKYASVEELMSIIYSVSISNIIFIIYSYFINYKVLESNYYRFPFTVHIIFW
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEECCHHHHHHHH
ILSIVSLGGTRFIYRVIEDKECNKNKCNCDSKEKKLLIIGAGDAAALIIKEIKRNKELNY
HHHHHHCCCHHEEHEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCE
SIIGLVDDDIEKLGKRINGIPVLGGRESIIKISKNKKIDEIILAIPSAASSTKSEIVSIC
EEEEEECHHHHHHHHHHCCCEEECCCCCEEEECCCCCCCHHEEECCCCCCHHHHHHHHHH
KETSCKLKTLPSMDKVIQGKFNMSKLRDVSIEDLLGREEVKLDDSGINRYIKDKVVLVTG
HHCCCCEECCCCHHHHHCCCCCHHHHCCCCHHHHCCCCCEEECHHHCCHHCCCEEEEEEC
GGGSIGSELCRQIVKFSPKKLLILDIYENNAYDVQMELNYKYPELDKAVIIASIRDEKRL
CCCCHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCEEEEEECHHHHHH
RNIFSLYKPDVVFHAAAHKHVPLMEENPAESIKNNVIGTYNLIKCAHEFGIERFVQISTD
HHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCHHHEEEECCC
KAVNPTNIMGATKRFCEIMIQAFDKISKTEFVAVRFGNVLGSNGSVIPLFKKQISHGGPV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCCCCCEEHHHHHHHCCCCCE
TVTHPEINRYFMTIPEAAQLVIQAGAMAKGGEIFVLDMGKPVKIVDLAKDLITLSGYKPE
EEECCCCCCEEEECCHHHHHHHHHCCCCCCCEEEEEECCCCEEHHHHHHHHHHHCCCCCC
EDIKIEYTGLRPGEKLYEELLMDEIALTSTGHNKIFVEKPMENNIDFVEKSIEEFKKVVY
CCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHC
NDEEEIFALIEKKVPTYIRKK
CCHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]