| Definition | Clostridium botulinum A str. ATCC 3502, complete genome. |
|---|---|
| Accession | NC_009495 |
| Length | 3,886,916 |
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The map label for this gene is banIIIM [H]
Identifier: 148381041
GI number: 148381041
Start: 3275627
End: 3277372
Strand: Reverse
Name: banIIIM [H]
Synonym: CBO3090
Alternate gene names: 148381041
Gene position: 3277372-3275627 (Counterclockwise)
Preceding gene: 148381043
Following gene: 148381040
Centisome position: 84.32
GC content: 22.16
Gene sequence:
>1746_bases GTGGTGAGTAATATGAATGCATTTAAAGATAATATCGAAAAAATTTTTAATATATTAATATCTCCTATAAATAGTATTTA CAAATATGAAGCTATTAATAACTTTAAATATAAGTTATCTATTGGTAAAAATGAGAATATATCCTTAAAATATTATGAAT TCATAAAGGGTAAAAAAGAAACTGGAGTTATATATACTCCACAAGAAATTTCTAATTACATGATAGAGAATACTATAAAT AAAGAGGATGTAATAAATAATCCCTTCATAAAAATATTAGATCCTTCCTGTGGTTGTGGAAATATTTTGATTCCTTGTTT TTTTTATTTAAAAAATATATTTGAAGAAAATTTGAAAGAGATAAATAAAAAAAACAATATAAATTTAGAAAAACAATATA TAAGTAAGCATATATTAGATAATAATTTATATGGCTTTGATATTGATACAATAGCTATAAAAATATTGATAATAGATTTG TTTTATTTAACAGGATATTATAACAAAAATAATTTTAAGAAAAAAGATTTTTTGATAGAAGATATAAATAATAACTTTGA TATATATATAGGTAATCCACCTTATGTTGGACATAAATCTGTAGATAAAGAATATTCAATGTTATTAAAAGAAAGATATG GATATATATATAAGGATAAAGGAGATATATCTTATTGTTTTTTTATAAATGCACTAAATTATTCTAACATAAATAGTAAA ATAACTTTTATAACATCAAGATATTTTATGGAGTCTAAAAGCGGGCATAATCTAAGAAAATACTTAAAAGAAAACTGTAA TATATATAAAATATTAGATTTTTATGGTATAAGACCCTTTAAGGCTGTAGGTATAGATCCAGCCATAATATTTATAGATA GAAATATTGGTAATAAAGTGGAAATTATAAAGCCTTGCAGATATGAGAAAGTAAAAATGGGTTTATTTTTTAATAATGAA GATAAGTACGAAAAATTTTATGTACATATGTCAGAGCTAAAACAAGATGGGTGGGTTTTGATAGATGATGGTAGTAGAGA TATAATAAATAAGATAGAGAATAAAACCAATAAAACTTTAGGAGAAATATGTACAAGTTATCAGGGAATTATTACCGGAT GTGATAAGGCTTTTATAGTTGATGAAAAAACTATAAAAAAGGAAAATTTGGAGAGGAGTATAATAAAGCCTTGGATAAAA AGTAGTTATATAAATAGAGAAAAAATTAATTTTAGAGATAGCTTTATAATATATTCAGATTTAATAGAAAATGTAAAAAA GTATCCTAATATAATAAGGCATATTGAAAAATATAAGGATAAATTAGAAAATAGGCGAGAATGCAAGAAGAAGGTTAGAA AGTGGTATGAGTTGCAATGGGGAAGAAAATTCGATATATTTGAAGATAAAAAAATAATATTTCCTTATAAGGCTAGTAAG AATAAATTTTATCTAGATAAAAAAAACGCATATTTTAGTGCAGATGTATATGCTTTGACCATAAATAAAGAAGAAGTCAT AGATTATAATAGTTTATTGATTATTTTAAACAGTGCTATATATGAGTTTTATTTTAAGACTTTTGGTAAAAAATTAGGTG GTAGTTTATATGAGTATTACCCTAATACATTAATGAAATTAAAGATACCAACGATTAAGATAAATAAAGAAGAGGATTTA TATAAATATTTTAATTTAAATGATAATGAAATAAAATTTATACAAAATAAGCTGCTAGTTAAGTAA
Upstream 100 bases:
>100_bases ATAAAAAAGAAGAAGCTAAAACACAAGAAATAACTTTTTTGTTCATAAGTAAATCACCTATCCTTTTCTATGTTATACTT TTTATAGAATAATAAAGATT
Downstream 100 bases:
>100_bases AAAGGTCTTTGCTTGAGCTACAAATATTTCTTCCTTAGAAGTTTTTATAGCTTCTTCATCTATTTTTCATAGTATTACTT TTTCCACATAAAGGTGTTTT
Product: modification methylase family protein
Products: NA
Alternate protein names: M.BanIII; Adenine-specific methyltransferase BanIII [H]
Number of amino acids: Translated: 581; Mature: 581
Protein sequence:
>581_residues MVSNMNAFKDNIEKIFNILISPINSIYKYEAINNFKYKLSIGKNENISLKYYEFIKGKKETGVIYTPQEISNYMIENTIN KEDVINNPFIKILDPSCGCGNILIPCFFYLKNIFEENLKEINKKNNINLEKQYISKHILDNNLYGFDIDTIAIKILIIDL FYLTGYYNKNNFKKKDFLIEDINNNFDIYIGNPPYVGHKSVDKEYSMLLKERYGYIYKDKGDISYCFFINALNYSNINSK ITFITSRYFMESKSGHNLRKYLKENCNIYKILDFYGIRPFKAVGIDPAIIFIDRNIGNKVEIIKPCRYEKVKMGLFFNNE DKYEKFYVHMSELKQDGWVLIDDGSRDIINKIENKTNKTLGEICTSYQGIITGCDKAFIVDEKTIKKENLERSIIKPWIK SSYINREKINFRDSFIIYSDLIENVKKYPNIIRHIEKYKDKLENRRECKKKVRKWYELQWGRKFDIFEDKKIIFPYKASK NKFYLDKKNAYFSADVYALTINKEEVIDYNSLLIILNSAIYEFYFKTFGKKLGGSLYEYYPNTLMKLKIPTIKINKEEDL YKYFNLNDNEIKFIQNKLLVK
Sequences:
>Translated_581_residues MVSNMNAFKDNIEKIFNILISPINSIYKYEAINNFKYKLSIGKNENISLKYYEFIKGKKETGVIYTPQEISNYMIENTIN KEDVINNPFIKILDPSCGCGNILIPCFFYLKNIFEENLKEINKKNNINLEKQYISKHILDNNLYGFDIDTIAIKILIIDL FYLTGYYNKNNFKKKDFLIEDINNNFDIYIGNPPYVGHKSVDKEYSMLLKERYGYIYKDKGDISYCFFINALNYSNINSK ITFITSRYFMESKSGHNLRKYLKENCNIYKILDFYGIRPFKAVGIDPAIIFIDRNIGNKVEIIKPCRYEKVKMGLFFNNE DKYEKFYVHMSELKQDGWVLIDDGSRDIINKIENKTNKTLGEICTSYQGIITGCDKAFIVDEKTIKKENLERSIIKPWIK SSYINREKINFRDSFIIYSDLIENVKKYPNIIRHIEKYKDKLENRRECKKKVRKWYELQWGRKFDIFEDKKIIFPYKASK NKFYLDKKNAYFSADVYALTINKEEVIDYNSLLIILNSAIYEFYFKTFGKKLGGSLYEYYPNTLMKLKIPTIKINKEEDL YKYFNLNDNEIKFIQNKLLVK >Mature_581_residues MVSNMNAFKDNIEKIFNILISPINSIYKYEAINNFKYKLSIGKNENISLKYYEFIKGKKETGVIYTPQEISNYMIENTIN KEDVINNPFIKILDPSCGCGNILIPCFFYLKNIFEENLKEINKKNNINLEKQYISKHILDNNLYGFDIDTIAIKILIIDL FYLTGYYNKNNFKKKDFLIEDINNNFDIYIGNPPYVGHKSVDKEYSMLLKERYGYIYKDKGDISYCFFINALNYSNINSK ITFITSRYFMESKSGHNLRKYLKENCNIYKILDFYGIRPFKAVGIDPAIIFIDRNIGNKVEIIKPCRYEKVKMGLFFNNE DKYEKFYVHMSELKQDGWVLIDDGSRDIINKIENKTNKTLGEICTSYQGIITGCDKAFIVDEKTIKKENLERSIIKPWIK SSYINREKINFRDSFIIYSDLIENVKKYPNIIRHIEKYKDKLENRRECKKKVRKWYELQWGRKFDIFEDKKIIFPYKASK NKFYLDKKNAYFSADVYALTINKEEVIDYNSLLIILNSAIYEFYFKTFGKKLGGSLYEYYPNTLMKLKIPTIKINKEEDL YKYFNLNDNEIKFIQNKLLVK
Specific function: This methylase recognizes the double-stranded sequence ATCGAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BanIII endonuclease [H]
COG id: COG1002
COG function: function code V; Type II restriction enzyme, methylase subunits
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003356 - InterPro: IPR002052 - InterPro: IPR002296 [H]
Pfam domain/function: PF02384 N6_Mtase [H]
EC number: =2.1.1.72 [H]
Molecular weight: Translated: 69146; Mature: 69146
Theoretical pI: Translated: 9.46; Mature: 9.46
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.5 %Cys (Translated Protein) 1.4 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 1.5 %Cys (Mature Protein) 1.4 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MVSNMNAFKDNIEKIFNILISPINSIYKYEAINNFKYKLSIGKNENISLKYYEFIKGKKE CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEHHHHCCCCC TGVIYTPQEISNYMIENTINKEDVINNPFIKILDPSCGCGNILIPCFFYLKNIFEENLKE CCEEECHHHHHHHHHHCCCCHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH INKKNNINLEKQYISKHILDNNLYGFDIDTIAIKILIIDLFYLTGYYNKNNFKKKDFLIE HCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCE DINNNFDIYIGNPPYVGHKSVDKEYSMLLKERYGYIYKDKGDISYCFFINALNYSNINSK ECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCCCCCE ITFITSRYFMESKSGHNLRKYLKENCNIYKILDFYGIRPFKAVGIDPAIIFIDRNIGNKV EEEEEEEHHHCCCCCCHHHHHHHCCCCEEEEEHHHCCCCEEECCCCEEEEEEECCCCCEE EIIKPCRYEKVKMGLFFNNEDKYEKFYVHMSELKQDGWVLIDDGSRDIINKIENKTNKTL EEECCCCCCEEEEEEEECCCCCHHHHEEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH GEICTSYQGIITGCDKAFIVDEKTIKKENLERSIIKPWIKSSYINREKINFRDSFIIYSD HHHHHHCCHHEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHH LIENVKKYPNIIRHIEKYKDKLENRRECKKKVRKWYELQWGRKFDIFEDKKIIFPYKASK HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEECCC NKFYLDKKNAYFSADVYALTINKEEVIDYNSLLIILNSAIYEFYFKTFGKKLGGSLYEYY CEEEEECCCCEEEEEEEEEEECHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHCCHHHHHC PNTLMKLKIPTIKINKEEDLYKYFNLNDNEIKFIQNKLLVK CCCEEEEEECEEEECCCCCHHHHCCCCCCHHHHHHHHHCCC >Mature Secondary Structure MVSNMNAFKDNIEKIFNILISPINSIYKYEAINNFKYKLSIGKNENISLKYYEFIKGKKE CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEHHHHCCCCC TGVIYTPQEISNYMIENTINKEDVINNPFIKILDPSCGCGNILIPCFFYLKNIFEENLKE CCEEECHHHHHHHHHHCCCCHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH INKKNNINLEKQYISKHILDNNLYGFDIDTIAIKILIIDLFYLTGYYNKNNFKKKDFLIE HCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCE DINNNFDIYIGNPPYVGHKSVDKEYSMLLKERYGYIYKDKGDISYCFFINALNYSNINSK ECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCCCCCE ITFITSRYFMESKSGHNLRKYLKENCNIYKILDFYGIRPFKAVGIDPAIIFIDRNIGNKV EEEEEEEHHHCCCCCCHHHHHHHCCCCEEEEEHHHCCCCEEECCCCEEEEEEECCCCCEE EIIKPCRYEKVKMGLFFNNEDKYEKFYVHMSELKQDGWVLIDDGSRDIINKIENKTNKTL EEECCCCCCEEEEEEEECCCCCHHHHEEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH GEICTSYQGIITGCDKAFIVDEKTIKKENLERSIIKPWIKSSYINREKINFRDSFIIYSD HHHHHHCCHHEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHH LIENVKKYPNIIRHIEKYKDKLENRRECKKKVRKWYELQWGRKFDIFEDKKIIFPYKASK HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEECCC NKFYLDKKNAYFSADVYALTINKEEVIDYNSLLIILNSAIYEFYFKTFGKKLGGSLYEYY CEEEEECCCCEEEEEEEEEEECHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHCCHHHHHC PNTLMKLKIPTIKINKEEDLYKYFNLNDNEIKFIQNKLLVK CCCEEEEEECEEEECCCCCHHHHCCCCCCHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 1368640 [H]