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The map label for this gene is ruvA

Identifier: 148381022

GI number: 148381022

Start: 3258387

End: 3258980

Strand: Reverse

Name: ruvA

Synonym: CBO3071

Alternate gene names: 148381022

Gene position: 3258980-3258387 (Counterclockwise)

Preceding gene: 148381023

Following gene: 148381021

Centisome position: 83.84

GC content: 26.94

Gene sequence:

>594_bases
GTGTACGAATATATAAAAGGAAAATATATTGATATGTACAAAGACTATATAGTTATAGAAAATAACAATATAGGTTATAA
GATATACACATCAGGAAGTACTATGGCAAAATTACCTTCCATAGGTGAAAATATAATGTTATATACTGAGCAAATAGTAA
GAGAAGATTTTATAGGAGTTTATGGATTTTTAACAAAAGATGAGTTAAGCATGTTTAAATTGCTTTTAACAATAAATGGG
GTAGGGGCTAAGGCAGCGTTATCTTTATTATCTATAAGCAATGTTTCTACTTTGAAATATGCTATAAAAATGGGAGATGA
AAAAACTATAACAAGAGCTCCTGGTATAGGTAAAAAAACAGCTCAAAGAATTATATTAGAGCTTAAGGATAAGATAGAGA
TAGATATTTTAGAAGAAGATGATGAACAAATCATAAATAAGGTTGCGGATGATAAAAAAGTATTAGAAGCAGTAGCTGCT
TTAGTAACACTAGGTTATTCTGAAAAAGAAGCAAATAAAGTGATTAATTCATGTGATAAAAATAATTCTTTAGAACAGAT
TATAAAGGAAGCTTTAAAATATTTAATGAAATAA

Upstream 100 bases:

>100_bases
ATTTACACTAATATGCAAGCTATGTTTTTTTAGTTGAATAAAAACTCCTAAAATGATAAAGTAATATATGTCTATTAATA
TGTAATTGGAGGGTTAAGGG

Downstream 100 bases:

>100_bases
ATTAGAGGGGGGTACTTATATGGAGAATAGAATGGTAACTCCTTTTGATGTAGAAGATGATAGGGAACAGTATAGTTTAA
GACCTACTACATTAAAAGAA

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MYEYIKGKYIDMYKDYIVIENNNIGYKIYTSGSTMAKLPSIGENIMLYTEQIVREDFIGVYGFLTKDELSMFKLLLTING
VGAKAALSLLSISNVSTLKYAIKMGDEKTITRAPGIGKKTAQRIILELKDKIEIDILEEDDEQIINKVADDKKVLEAVAA
LVTLGYSEKEANKVINSCDKNNSLEQIIKEALKYLMK

Sequences:

>Translated_197_residues
MYEYIKGKYIDMYKDYIVIENNNIGYKIYTSGSTMAKLPSIGENIMLYTEQIVREDFIGVYGFLTKDELSMFKLLLTING
VGAKAALSLLSISNVSTLKYAIKMGDEKTITRAPGIGKKTAQRIILELKDKIEIDILEEDDEQIINKVADDKKVLEAVAA
LVTLGYSEKEANKVINSCDKNNSLEQIIKEALKYLMK
>Mature_197_residues
MYEYIKGKYIDMYKDYIVIENNNIGYKIYTSGSTMAKLPSIGENIMLYTEQIVREDFIGVYGFLTKDELSMFKLLLTING
VGAKAALSLLSISNVSTLKYAIKMGDEKTITRAPGIGKKTAQRIILELKDKIEIDILEEDDEQIINKVADDKKVLEAVAA
LVTLGYSEKEANKVINSCDKNNSLEQIIKEALKYLMK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=200, Percent_Identity=33.5, Blast_Score=116, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_CLOB1 (A7FY20)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385393.1
- ProteinModelPortal:   A7FY20
- SMR:   A7FY20
- STRING:   A7FY20
- GeneID:   5397741
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3100
- eggNOG:   COG0632
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- ProtClustDB:   PRK00116
- BioCyc:   CBOT441770:CLB_3100-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 22183; Mature: 22183

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEYIKGKYIDMYKDYIVIENNNIGYKIYTSGSTMAKLPSIGENIMLYTEQIVREDFIGV
CCCCCCCCEEEEECCEEEEECCCCCEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHHHHH
YGFLTKDELSMFKLLLTINGVGAKAALSLLSISNVSTLKYAIKMGDEKTITRAPGIGKKT
HHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCEECCCCCCHHH
AQRIILELKDKIEIDILEEDDEQIINKVADDKKVLEAVAALVTLGYSEKEANKVINSCDK
HHHHHHHHHCCEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
NNSLEQIIKEALKYLMK
CCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MYEYIKGKYIDMYKDYIVIENNNIGYKIYTSGSTMAKLPSIGENIMLYTEQIVREDFIGV
CCCCCCCCEEEEECCEEEEECCCCCEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHHHHH
YGFLTKDELSMFKLLLTINGVGAKAALSLLSISNVSTLKYAIKMGDEKTITRAPGIGKKT
HHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCEECCCCCCHHH
AQRIILELKDKIEIDILEEDDEQIINKVADDKKVLEAVAALVTLGYSEKEANKVINSCDK
HHHHHHHHHCCEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
NNSLEQIIKEALKYLMK
CCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA