Click here to switch to the map view.

The map label for this gene is queA

Identifier: 148381020

GI number: 148381020

Start: 3256280

End: 3257305

Strand: Reverse

Name: queA

Synonym: CBO3069

Alternate gene names: 148381020

Gene position: 3257305-3256280 (Counterclockwise)

Preceding gene: 148381021

Following gene: 148381019

Centisome position: 83.8

GC content: 31.77

Gene sequence:

>1026_bases
GTGAAAGTAAAAGATTTTGATTTTTATTTACCAGAGGAATTAATAGCACAACATCCTATAGAGAAAAGAGATGAAGCCAG
ACTACTTGTATTAGATAAGGAAACAGGTGAAATAGAACATAAAATATTTAAGGATATTTTAGACTATTTAACACCAAATG
ATTGTTTAGTTCTAAATAATACTAGAGTTTTACCTGCAAGACTTATAGGAGCAAAAGAAGAAACCGGTGGGAAAATGGAA
TTCTTGCTTTTAAAAAGAAAAGAAAAAGATGTATGGGAAACTTTAGTTAAACCAGGAAAGAGAGCTCAGATTGGAGCAAG
ATTTATATTTGGCAACGGAGAGCTTAAAGCAGAAGTTATAGGAATGGGAGAAGAGGGAAGTAGAATAGTAAAATTTTATT
ATGAAGGTATTTTCGAGGAAATATTAGATCAACTAGGACAAATGCCGCTACCTCCATATATAAAGGAAAAACTAGATGAT
AAAGAAATGTATCAAACAGTTTATTCAAAGGAAGAGGGATCGGCAGCAGCTCCTACAGCAGGACTTCATTTTACAGAGGA
ACTTTTGAAAAAAATAGAAGAAAAGGGTGTGAAATTAGCCTTTTTAACCTTACATGTAGGATTAGGAACTTTTAGACCAG
TTAAAGTAGAGGATATTCAAGAACATGTTATGCATTCAGAATACTATAAAATGGATAAAAAAACTGCTGAAATAATAAAT
GATACAAAAGAAAATGGTGGAAGAGTTATAGCAGTAGGAACTACTTCTTGTAGAACATTGGAAACTATAGGAGCCATAGA
AGGTAAAGTAGGAGAACAATCAGGATGGACAGATATATTTATTTATCCCGGATATAAATACAAAGTAGTGGATGCTTTAA
TTACTAATTTCCATCTTCCACAATCTACATTATTGATGTTAGTATCTGCATTAGCAGGAAGAGACAATATAATGAATGCA
TATAATGTTGCAGTAGAAAAGGAATATAGATTCTTCTCTTTTGGAGATGCTATGTTTATTAAATAA

Upstream 100 bases:

>100_bases
ATATAACCATTTTGGAGTAACAAAAAAAGAAGAAAAGGATAATCAAGTATCTATATTTAATAAATAATAAATTTTAATTC
TATATTAAGAGGTGGAAGAC

Downstream 100 bases:

>100_bases
TATTATTAAGTAATTTTAATAGAAGCGTAATCCCTAAGGAAAAAGAGGTGTAAGTATGTACAAATTGTTAAAAAAATCAG
GAAAGGCAAGACGAGGAGAA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKVKDFDFYLPEELIAQHPIEKRDEARLLVLDKETGEIEHKIFKDILDYLTPNDCLVLNNTRVLPARLIGAKEETGGKME
FLLLKRKEKDVWETLVKPGKRAQIGARFIFGNGELKAEVIGMGEEGSRIVKFYYEGIFEEILDQLGQMPLPPYIKEKLDD
KEMYQTVYSKEEGSAAAPTAGLHFTEELLKKIEEKGVKLAFLTLHVGLGTFRPVKVEDIQEHVMHSEYYKMDKKTAEIIN
DTKENGGRVIAVGTTSCRTLETIGAIEGKVGEQSGWTDIFIYPGYKYKVVDALITNFHLPQSTLLMLVSALAGRDNIMNA
YNVAVEKEYRFFSFGDAMFIK

Sequences:

>Translated_341_residues
MKVKDFDFYLPEELIAQHPIEKRDEARLLVLDKETGEIEHKIFKDILDYLTPNDCLVLNNTRVLPARLIGAKEETGGKME
FLLLKRKEKDVWETLVKPGKRAQIGARFIFGNGELKAEVIGMGEEGSRIVKFYYEGIFEEILDQLGQMPLPPYIKEKLDD
KEMYQTVYSKEEGSAAAPTAGLHFTEELLKKIEEKGVKLAFLTLHVGLGTFRPVKVEDIQEHVMHSEYYKMDKKTAEIIN
DTKENGGRVIAVGTTSCRTLETIGAIEGKVGEQSGWTDIFIYPGYKYKVVDALITNFHLPQSTLLMLVSALAGRDNIMNA
YNVAVEKEYRFFSFGDAMFIK
>Mature_341_residues
MKVKDFDFYLPEELIAQHPIEKRDEARLLVLDKETGEIEHKIFKDILDYLTPNDCLVLNNTRVLPARLIGAKEETGGKME
FLLLKRKEKDVWETLVKPGKRAQIGARFIFGNGELKAEVIGMGEEGSRIVKFYYEGIFEEILDQLGQMPLPPYIKEKLDD
KEMYQTVYSKEEGSAAAPTAGLHFTEELLKKIEEKGVKLAFLTLHVGLGTFRPVKVEDIQEHVMHSEYYKMDKKTAEIIN
DTKENGGRVIAVGTTSCRTLETIGAIEGKVGEQSGWTDIFIYPGYKYKVVDALITNFHLPQSTLLMLVSALAGRDNIMNA
YNVAVEKEYRFFSFGDAMFIK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=344, Percent_Identity=50, Blast_Score=331, Evalue=3e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_CLOB1 (A7FY18)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385391.1
- ProteinModelPortal:   A7FY18
- SMR:   A7FY18
- STRING:   A7FY18
- GeneID:   5394829
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3098
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- ProtClustDB:   PRK00147
- BioCyc:   CBOT441770:CLB_3098-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38748; Mature: 38748

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKDFDFYLPEELIAQHPIEKRDEARLLVLDKETGEIEHKIFKDILDYLTPNDCLVLNN
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECC
TRVLPARLIGAKEETGGKMEFLLLKRKEKDVWETLVKPGKRAQIGARFIFGNGELKAEVI
CEECHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCEECEEEEEECCCEEEEEE
GMGEEGSRIVKFYYEGIFEEILDQLGQMPLPPYIKEKLDDKEMYQTVYSKEEGSAAAPTA
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCC
GLHFTEELLKKIEEKGVKLAFLTLHVGLGTFRPVKVEDIQEHVMHSEYYKMDKKTAEIIN
CHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DTKENGGRVIAVGTTSCRTLETIGAIEGKVGEQSGWTDIFIYPGYKYKVVDALITNFHLP
HHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCC
QSTLLMLVSALAGRDNIMNAYNVAVEKEYRFFSFGDAMFIK
HHHHHHHHHHHCCCCHHHHHHHEEEECCEEEEEECCEEEEC
>Mature Secondary Structure
MKVKDFDFYLPEELIAQHPIEKRDEARLLVLDKETGEIEHKIFKDILDYLTPNDCLVLNN
CCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECC
TRVLPARLIGAKEETGGKMEFLLLKRKEKDVWETLVKPGKRAQIGARFIFGNGELKAEVI
CEECHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCEECEEEEEECCCEEEEEE
GMGEEGSRIVKFYYEGIFEEILDQLGQMPLPPYIKEKLDDKEMYQTVYSKEEGSAAAPTA
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCC
GLHFTEELLKKIEEKGVKLAFLTLHVGLGTFRPVKVEDIQEHVMHSEYYKMDKKTAEIIN
CHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DTKENGGRVIAVGTTSCRTLETIGAIEGKVGEQSGWTDIFIYPGYKYKVVDALITNFHLP
HHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCC
QSTLLMLVSALAGRDNIMNAYNVAVEKEYRFFSFGDAMFIK
HHHHHHHHHHHCCCCHHHHHHHEEEECCEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA