Click here to switch to the map view.

The map label for this gene is tgt

Identifier: 148381019

GI number: 148381019

Start: 3255094

End: 3256224

Strand: Reverse

Name: tgt

Synonym: CBO3068

Alternate gene names: 148381019

Gene position: 3256224-3255094 (Counterclockwise)

Preceding gene: 148381020

Following gene: 148381018

Centisome position: 83.77

GC content: 32.54

Gene sequence:

>1131_bases
ATGTACAAATTGTTAAAAAAATCAGGAAAGGCAAGACGAGGAGAATTTACGACTCCTCATGGGGTGATACAGACTCCTGT
ATTTATGAATGTAGGAACCCTTGCGGCTATAAAAGGGGCAGTATCATCTATGGATTTAAAGGAAATAGGATGCCAAGTTG
AGTTATCTAATACATACCATCTTCATTTAAGACCAGGAGATGAAGTTGTTAAAAAAATGGGTGGATTACATAAATTTATG
AATTGGGATAGACCAATACTTACAGATTCAGGTGGATTTCAAGTATTTTCATTATCAAAAATAAGAAAAATACAAGAAGA
AGGAGTTTATTTTAATTCTCATATAGATGGAAGAAAAATATTTATGGGACCGGAAGAAAGTATGAGAATACAAAGCAATC
TAGCCTCAACTATAGCTATGGCTTTTGATGAATGTGTAGAAAATCCAGCACCAAGAGAGTATGTAGAAAAGTCCGTAGAA
AGAACAACAAGATGGTTACATAGATGTAAGGATGAAATGAATAGATTAAATTCTTTGCCAGATACTATAAATAATAAGCA
AATGTTATTTGGTATAAATCAAGGTGGAACTTATGAAGATATAAGAATAGAACATGCTAAAACTATAGCTAAAATGGATC
TAGATGGATATGCTATAGGGGGATTAGCAGTTGGAGAAAGTCATGAAGATATGTATAGAATAATAGATGCAGTGGTTCCC
CACCTTCCAGAAGATAAACCCATATATCTTATGGGAGTTGGAATACCAAGTAATATATTAGAAGCTGTAGATAGGGGTGT
AGATTTCTTTGACTGTGTTCTTCCTGCAAGAAATGGAAGACATGCACATGTTTTCACAAAAGAGGGCAAAATAAATTTAT
TGAATGCCAAATTTGAATTAGATGATAGACCTATTGATGAAGGTTGTCAATGTCCAGCTTGTAAGCATTATACAAGGTCA
TATATAAGACATTTATTTAAAGCAAAGGAAATGCTAGCTATGAGATTATGTGTTTTACATAACTTATATTTTTATAATAA
CTTAATGGAAGAAATAAGAGATGCCATTGATGGAAATTATTTTAAAGAATACAAAGAAAGAAAGCTTAAAGAGTGGGGAG
GAAGAGCATAA

Upstream 100 bases:

>100_bases
GAATATAGATTCTTCTCTTTTGGAGATGCTATGTTTATTAAATAATATTATTAAGTAATTTTAATAGAAGCGTAATCCCT
AAGGAAAAAGAGGTGTAAGT

Downstream 100 bases:

>100_bases
AGAATTAAAAATAATTTTTGTGTATTAAATGCTATAACAAATTTTATACTTTAAATATAAAATAATATAAATATATCTAT
TAATTGTAAATTATCAATTA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MYKLLKKSGKARRGEFTTPHGVIQTPVFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYHLHLRPGDEVVKKMGGLHKFM
NWDRPILTDSGGFQVFSLSKIRKIQEEGVYFNSHIDGRKIFMGPEESMRIQSNLASTIAMAFDECVENPAPREYVEKSVE
RTTRWLHRCKDEMNRLNSLPDTINNKQMLFGINQGGTYEDIRIEHAKTIAKMDLDGYAIGGLAVGESHEDMYRIIDAVVP
HLPEDKPIYLMGVGIPSNILEAVDRGVDFFDCVLPARNGRHAHVFTKEGKINLLNAKFELDDRPIDEGCQCPACKHYTRS
YIRHLFKAKEMLAMRLCVLHNLYFYNNLMEEIRDAIDGNYFKEYKERKLKEWGGRA

Sequences:

>Translated_376_residues
MYKLLKKSGKARRGEFTTPHGVIQTPVFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYHLHLRPGDEVVKKMGGLHKFM
NWDRPILTDSGGFQVFSLSKIRKIQEEGVYFNSHIDGRKIFMGPEESMRIQSNLASTIAMAFDECVENPAPREYVEKSVE
RTTRWLHRCKDEMNRLNSLPDTINNKQMLFGINQGGTYEDIRIEHAKTIAKMDLDGYAIGGLAVGESHEDMYRIIDAVVP
HLPEDKPIYLMGVGIPSNILEAVDRGVDFFDCVLPARNGRHAHVFTKEGKINLLNAKFELDDRPIDEGCQCPACKHYTRS
YIRHLFKAKEMLAMRLCVLHNLYFYNNLMEEIRDAIDGNYFKEYKERKLKEWGGRA
>Mature_376_residues
MYKLLKKSGKARRGEFTTPHGVIQTPVFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYHLHLRPGDEVVKKMGGLHKFM
NWDRPILTDSGGFQVFSLSKIRKIQEEGVYFNSHIDGRKIFMGPEESMRIQSNLASTIAMAFDECVENPAPREYVEKSVE
RTTRWLHRCKDEMNRLNSLPDTINNKQMLFGINQGGTYEDIRIEHAKTIAKMDLDGYAIGGLAVGESHEDMYRIIDAVVP
HLPEDKPIYLMGVGIPSNILEAVDRGVDFFDCVLPARNGRHAHVFTKEGKINLLNAKFELDDRPIDEGCQCPACKHYTRS
YIRHLFKAKEMLAMRLCVLHNLYFYNNLMEEIRDAIDGNYFKEYKERKLKEWGGRA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=40.8450704225352, Blast_Score=289, Evalue=3e-78,
Organism=Homo sapiens, GI13375872, Length=254, Percent_Identity=27.5590551181102, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1786607, Length=374, Percent_Identity=45.7219251336898, Blast_Score=356, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17544680, Length=374, Percent_Identity=40.3743315508021, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI25146502, Length=140, Percent_Identity=27.8571428571429, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920510, Length=365, Percent_Identity=41.0958904109589, Blast_Score=291, Evalue=4e-79,
Organism=Drosophila melanogaster, GI21355311, Length=228, Percent_Identity=25.8771929824561, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_CLOB1 (A7FY17)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385390.1
- ProteinModelPortal:   A7FY17
- SMR:   A7FY17
- STRING:   A7FY17
- GeneID:   5394736
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3097
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BioCyc:   CBOT441770:CLB_3097-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 42940; Mature: 42940

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: ACT_SITE 89-89 BINDING 90-90

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKLLKKSGKARRGEFTTPHGVIQTPVFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYH
CCHHHHHCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCHHHCCCEEEECCEEE
LHLRPGDEVVKKMGGLHKFMNWDRPILTDSGGFQVFSLSKIRKIQEEGVYFNSHIDGRKI
EEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHCCCEEECCCCCCEE
FMGPEESMRIQSNLASTIAMAFDECVENPAPREYVEKSVERTTRWLHRCKDEMNRLNSLP
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DTINNKQMLFGINQGGTYEDIRIEHAKTIAKMDLDGYAIGGLAVGESHEDMYRIIDAVVP
CCCCCCEEEEEECCCCCCHHEEHHHHHHHHHCCCCCEEECCEEECCCHHHHHHHHHHHHC
HLPEDKPIYLMGVGIPSNILEAVDRGVDFFDCVLPARNGRHAHVFTKEGKINLLNAKFEL
CCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCEEEEEEEEEC
DDRPIDEGCQCPACKHYTRSYIRHLFKAKEMLAMRLCVLHNLYFYNNLMEEIRDAIDGNY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
FKEYKERKLKEWGGRA
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYKLLKKSGKARRGEFTTPHGVIQTPVFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYH
CCHHHHHCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCHHHCCCEEEECCEEE
LHLRPGDEVVKKMGGLHKFMNWDRPILTDSGGFQVFSLSKIRKIQEEGVYFNSHIDGRKI
EEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHCCCEEECCCCCCEE
FMGPEESMRIQSNLASTIAMAFDECVENPAPREYVEKSVERTTRWLHRCKDEMNRLNSLP
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DTINNKQMLFGINQGGTYEDIRIEHAKTIAKMDLDGYAIGGLAVGESHEDMYRIIDAVVP
CCCCCCEEEEEECCCCCCHHEEHHHHHHHHHCCCCCEEECCEEECCCHHHHHHHHHHHHC
HLPEDKPIYLMGVGIPSNILEAVDRGVDFFDCVLPARNGRHAHVFTKEGKINLLNAKFEL
CCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCEEEEEEEEEC
DDRPIDEGCQCPACKHYTRSYIRHLFKAKEMLAMRLCVLHNLYFYNNLMEEIRDAIDGNY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
FKEYKERKLKEWGGRA
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA