| Definition | Clostridium botulinum A str. ATCC 3502, complete genome. |
|---|---|
| Accession | NC_009495 |
| Length | 3,886,916 |
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The map label for this gene is polA [H]
Identifier: 148380963
GI number: 148380963
Start: 3204018
End: 3206645
Strand: Reverse
Name: polA [H]
Synonym: CBO3014
Alternate gene names: 148380963
Gene position: 3206645-3204018 (Counterclockwise)
Preceding gene: 148380964
Following gene: 148380962
Centisome position: 82.5
GC content: 27.21
Gene sequence:
>2628_bases ATGAATAAAGAAAGATTATTAATATTAGATAGTAATAGTTTATTAAATAGAGCTTTTTATGCATTACCTGATCTTATGAC TGGAGAGGGAATTCATACTAATGCTATATATGGTTTTGTTAATATGCTTTTAAAAATGAAAGAAGAGATAAACCCAGATT ATATAGTTGCAGCTTTTGATAGAAAGGCACCTACCTTTAGACATGAAGAATATAAGGACTACAAAGCAGGTAGAAAAAAG ATGCCAGAGGAACTTGCACAGCAGTTTCCTATAGTAAAAGAGCTTTTGTCCGCATTAGCCATAAATATATTCGAAATTGA TGGGTTTGAAGCAGACGATCTAATAGGAACTTTATCTGTATTTGCAGAGGAAAAAGGAATAGAAGTTTACATAGTTACAG GAGATAAAGATGCCCTACAATTGGCTACAGATAATGTAAAAGTAGTTATAACTAAAAAGGGTATTACAGAAAAAGAAATT TATGATAAAAATAGAATGGTAGAGGAGTTTGGAGTTACTCCTAAAGAATTTATAGATGTAAAAGGTCTTATGGGAGACAC ATCTGACAATATACCTGGAGTACCTGGAATAGGTGAAAAAACTGCCTTTAAGCTTATAAAAGAATATAAAAGTATAGAAA CAGTACTTGAAAACATAGAGAATATAAAAGGAAAAAAATTAAAGGAAAATTTACATGAATATAGAGAACAGGCTATATTT AGCAAAAAATTAGCTACTATTATATGTAATGTTCCTATAGATATGAGTTTAGAAGATATAAAATCAAAGGAAGAATATGA TATAAACAAAGTTAGAGAAATGTTTGAAAGGTTAGAATTTAAGTCTTTAATGAATAAAGTAGGTAAAAGTGATGAGGAAG AAACGGTAGAAGAGGAAGTTTCTTATAAAAATATAGTTTCTTTAGAAGAATTTAAAGATCTAAAAAATAATATAATAAAA TATAAAGAAAATGATTTATATCTGTATTTTGAATTAGAAGATATAGTTTTATTTTCTAAATCTAGAGTAAAAACATTATA TGCTAATTTTAAAGATGAAGTTTATAGAATAGACTTTGAAATGCTTATAAAAAATACTAAAGAACAATTTATAGAAGTAT GTAAAGAAATATTTGAAAGTAAAGAAATTAAAAAAATTACCTATGATGCAAAAAATCCAAGGACTATATTAAGAAAGTTA GGAATAGAGTTTAATGGTATAAAATTTGATATAAATTTAGCTGCATATCTAATAGATCCAGTGAGGAAAGAATATGAGAT ATCTAGTTTAGCTTCAGAATACTTGTTTAAAAATATAAACAGAGAAGATGAATTTTTAAAAATAAAAGAAGTTAATATAA TGCCTAATTTATATAAAGTGCTGGAAGAAAAAATAAAAGAGCAAGATATGGAAGAATTGCTATATAAGGTAGAACAACCT CTTACAGAAACATTATCAGCTATGGAGACAGAAGGTTTTAAAGTTGATAAAGATATACTTTTACAGCTAGAGAAAAAATT TAAAGGGGAAATAGAAAGTACTCAGCTAGAAATATATTCAATGTCTGAAGAAGAATTTAATATAAATTCTCCTAAACAGT TAGGGAAAATACTCTTTGAAAAATTAGATTTACCCGTTATAAAGAAAACAAAGACAGGATATTCTACTAACGCAGAGGTA CTGGATAAATTAAGAGATAAACATCCAATAATAGATAAAATAACTTATTATAGACAGTTAACAAAAATATACTCTACTTA TATAGAAGGGCTAAAAGCTGCTATAGATGAGGATGGAAAAATACATTCAAATTTTAATCAAACTGTTACAGCTACAGGAA GATTATCTAGTACAGAGCCTAATCTTCAAAATATACCTATAAAATATGAAATGGGAAGAGAAATAAGAAAGGTATTTATC CCTAATACAGGGGATTCTGTAATACTTTCAGCGGATTATTCACAAATAGAATTAAGAGTATTAGCACATATATCTGATGA TAAAAATATGATAAGTGCCTTTAATGAACATGATGATATTCATACTAAAACTGCATCAGAAGTGTTTAAAGTTCCAATAG AAGAAGTAACACCTCTTATGAGAGGTAATGCTAAAGCAGTTAATTTTGGTATAGTTTATGGTATAGGAGACTTTAGCTTA TCTCAAGATTTAAATATAACTAGAAAAGAAGCAAAGCAGTATATAGATGCTTATTTGGAGAGATATCCTAATGTAAAACT TTATTTAGAAAATATAGTAGAAGAAGCTATTGAAAAAGGTTATGTAAGTACTATATTAAATAGAAGAAGATACATAAAAG AGGTTAAGTCCTCAAATAAAATAGTTAAAGCTGCAGGAGAAAGGCTAGCTATGAATAGTCCAATACAAGGAAGTGCAGCG GATATAATAAAATTAGCTATGGTAAATGTTCATAGAAAATTAAAAGAAGATAATTTTAAAAGTAGTATAATATTACAAGT ACATGATGAATTAATATTAAATGTCCATAAAGATGAACTAGAAAAAATCAAAGCTTTAGTAAAAAAAGAAATGGAACAGG TTTTAGAATTAAAAGTTGGGTTAGACGTGGATATAAACATAGGTAATAATTGGTATGAGGCAAAGTAG
Upstream 100 bases:
>100_bases CATTTGAAATATTAAAAGAAATTTTGAATACATATCCCTGTGATGATTTCAATATGTTAAAAAAATACATGAGAGAAATG TAAAATTTGGAGGTTCATTT
Downstream 100 bases:
>100_bases GGAGTTGTTTTATGTATTGCTTAAAAAGTATTTTTAAAATTGGTCTTACAGGTGGTATAGGTGCTGGTAAAAGTACTATA TCAGAAATGATAAAAGAAAA
Product: DNA polymerase I
Products: NA
Alternate protein names: POL I [H]
Number of amino acids: Translated: 875; Mature: 875
Protein sequence:
>875_residues MNKERLLILDSNSLLNRAFYALPDLMTGEGIHTNAIYGFVNMLLKMKEEINPDYIVAAFDRKAPTFRHEEYKDYKAGRKK MPEELAQQFPIVKELLSALAINIFEIDGFEADDLIGTLSVFAEEKGIEVYIVTGDKDALQLATDNVKVVITKKGITEKEI YDKNRMVEEFGVTPKEFIDVKGLMGDTSDNIPGVPGIGEKTAFKLIKEYKSIETVLENIENIKGKKLKENLHEYREQAIF SKKLATIICNVPIDMSLEDIKSKEEYDINKVREMFERLEFKSLMNKVGKSDEEETVEEEVSYKNIVSLEEFKDLKNNIIK YKENDLYLYFELEDIVLFSKSRVKTLYANFKDEVYRIDFEMLIKNTKEQFIEVCKEIFESKEIKKITYDAKNPRTILRKL GIEFNGIKFDINLAAYLIDPVRKEYEISSLASEYLFKNINREDEFLKIKEVNIMPNLYKVLEEKIKEQDMEELLYKVEQP LTETLSAMETEGFKVDKDILLQLEKKFKGEIESTQLEIYSMSEEEFNINSPKQLGKILFEKLDLPVIKKTKTGYSTNAEV LDKLRDKHPIIDKITYYRQLTKIYSTYIEGLKAAIDEDGKIHSNFNQTVTATGRLSSTEPNLQNIPIKYEMGREIRKVFI PNTGDSVILSADYSQIELRVLAHISDDKNMISAFNEHDDIHTKTASEVFKVPIEEVTPLMRGNAKAVNFGIVYGIGDFSL SQDLNITRKEAKQYIDAYLERYPNVKLYLENIVEEAIEKGYVSTILNRRRYIKEVKSSNKIVKAAGERLAMNSPIQGSAA DIIKLAMVNVHRKLKEDNFKSSIILQVHDELILNVHKDELEKIKALVKKEMEQVLELKVGLDVDINIGNNWYEAK
Sequences:
>Translated_875_residues MNKERLLILDSNSLLNRAFYALPDLMTGEGIHTNAIYGFVNMLLKMKEEINPDYIVAAFDRKAPTFRHEEYKDYKAGRKK MPEELAQQFPIVKELLSALAINIFEIDGFEADDLIGTLSVFAEEKGIEVYIVTGDKDALQLATDNVKVVITKKGITEKEI YDKNRMVEEFGVTPKEFIDVKGLMGDTSDNIPGVPGIGEKTAFKLIKEYKSIETVLENIENIKGKKLKENLHEYREQAIF SKKLATIICNVPIDMSLEDIKSKEEYDINKVREMFERLEFKSLMNKVGKSDEEETVEEEVSYKNIVSLEEFKDLKNNIIK YKENDLYLYFELEDIVLFSKSRVKTLYANFKDEVYRIDFEMLIKNTKEQFIEVCKEIFESKEIKKITYDAKNPRTILRKL GIEFNGIKFDINLAAYLIDPVRKEYEISSLASEYLFKNINREDEFLKIKEVNIMPNLYKVLEEKIKEQDMEELLYKVEQP LTETLSAMETEGFKVDKDILLQLEKKFKGEIESTQLEIYSMSEEEFNINSPKQLGKILFEKLDLPVIKKTKTGYSTNAEV LDKLRDKHPIIDKITYYRQLTKIYSTYIEGLKAAIDEDGKIHSNFNQTVTATGRLSSTEPNLQNIPIKYEMGREIRKVFI PNTGDSVILSADYSQIELRVLAHISDDKNMISAFNEHDDIHTKTASEVFKVPIEEVTPLMRGNAKAVNFGIVYGIGDFSL SQDLNITRKEAKQYIDAYLERYPNVKLYLENIVEEAIEKGYVSTILNRRRYIKEVKSSNKIVKAAGERLAMNSPIQGSAA DIIKLAMVNVHRKLKEDNFKSSIILQVHDELILNVHKDELEKIKALVKKEMEQVLELKVGLDVDINIGNNWYEAK >Mature_875_residues MNKERLLILDSNSLLNRAFYALPDLMTGEGIHTNAIYGFVNMLLKMKEEINPDYIVAAFDRKAPTFRHEEYKDYKAGRKK MPEELAQQFPIVKELLSALAINIFEIDGFEADDLIGTLSVFAEEKGIEVYIVTGDKDALQLATDNVKVVITKKGITEKEI YDKNRMVEEFGVTPKEFIDVKGLMGDTSDNIPGVPGIGEKTAFKLIKEYKSIETVLENIENIKGKKLKENLHEYREQAIF SKKLATIICNVPIDMSLEDIKSKEEYDINKVREMFERLEFKSLMNKVGKSDEEETVEEEVSYKNIVSLEEFKDLKNNIIK YKENDLYLYFELEDIVLFSKSRVKTLYANFKDEVYRIDFEMLIKNTKEQFIEVCKEIFESKEIKKITYDAKNPRTILRKL GIEFNGIKFDINLAAYLIDPVRKEYEISSLASEYLFKNINREDEFLKIKEVNIMPNLYKVLEEKIKEQDMEELLYKVEQP LTETLSAMETEGFKVDKDILLQLEKKFKGEIESTQLEIYSMSEEEFNINSPKQLGKILFEKLDLPVIKKTKTGYSTNAEV LDKLRDKHPIIDKITYYRQLTKIYSTYIEGLKAAIDEDGKIHSNFNQTVTATGRLSSTEPNLQNIPIKYEMGREIRKVFI PNTGDSVILSADYSQIELRVLAHISDDKNMISAFNEHDDIHTKTASEVFKVPIEEVTPLMRGNAKAVNFGIVYGIGDFSL SQDLNITRKEAKQYIDAYLERYPNVKLYLENIVEEAIEKGYVSTILNRRRYIKEVKSSNKIVKAAGERLAMNSPIQGSAA DIIKLAMVNVHRKLKEDNFKSSIILQVHDELILNVHKDELEKIKALVKKEMEQVLELKVGLDVDINIGNNWYEAK
Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]
COG id: COG0749
COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 5'-3' exonuclease domain [H]
Homologues:
Organism=Homo sapiens, GI112420958, Length=510, Percent_Identity=32.3529411764706, Blast_Score=254, Evalue=3e-67, Organism=Homo sapiens, GI139394648, Length=646, Percent_Identity=25.6965944272446, Blast_Score=220, Evalue=6e-57, Organism=Escherichia coli, GI1790294, Length=925, Percent_Identity=36.4324324324324, Blast_Score=536, Evalue=1e-153, Organism=Escherichia coli, GI226510970, Length=212, Percent_Identity=32.5471698113208, Blast_Score=127, Evalue=4e-30, Organism=Caenorhabditis elegans, GI86563272, Length=392, Percent_Identity=29.8469387755102, Blast_Score=182, Evalue=5e-46, Organism=Drosophila melanogaster, GI17933644, Length=426, Percent_Identity=34.037558685446, Blast_Score=229, Evalue=5e-60,
Paralogues:
None
Copy number: 400 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002562 - InterPro: IPR020046 - InterPro: IPR020045 - InterPro: IPR002421 - InterPro: IPR020047 - InterPro: IPR019760 - InterPro: IPR001098 - InterPro: IPR018320 - InterPro: IPR002298 - InterPro: IPR008918 - InterPro: IPR003583 - InterPro: IPR012337 [H]
Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 101036; Mature: 101036
Theoretical pI: Translated: 5.06; Mature: 5.06
Prosite motif: PS00447 DNA_POLYMERASE_A
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNKERLLILDSNSLLNRAFYALPDLMTGEGIHTNAIYGFVNMLLKMKEEINPDYIVAAFD CCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEC RKAPTFRHEEYKDYKAGRKKMPEELAQQFPIVKELLSALAINIFEIDGFEADDLIGTLSV CCCCCCCCHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHEEEEECCCCHHHHHHHHHH FAEEKGIEVYIVTGDKDALQLATDNVKVVITKKGITEKEIYDKNRMVEEFGVTPKEFIDV HHCCCCCEEEEEECCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHH KGLMGDTSDNIPGVPGIGEKTAFKLIKEYKSIETVLENIENIKGKKLKENLHEYREQAIF HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH SKKLATIICNVPIDMSLEDIKSKEEYDINKVREMFERLEFKSLMNKVGKSDEEETVEEEV HHHHHHHEECCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH SYKNIVSLEEFKDLKNNIIKYKENDLYLYFELEDIVLFSKSRVKTLYANFKDEVYRIDFE HHHHHHHHHHHHHHHHHHCEEECCCEEEEEEEHHHHEECHHHHHHHHHHHCHHHHHHHHH MLIKNTKEQFIEVCKEIFESKEIKKITYDAKNPRTILRKLGIEFNGIKFDINLAAYLIDP HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHH VRKEYEISSLASEYLFKNINREDEFLKIKEVNIMPNLYKVLEEKIKEQDMEELLYKVEQP HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH LTETLSAMETEGFKVDKDILLQLEKKFKGEIESTQLEIYSMSEEEFNINSPKQLGKILFE HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH KLDLPVIKKTKTGYSTNAEVLDKLRDKHPIIDKITYYRQLTKIYSTYIEGLKAAIDEDGK HCCCCEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC IHSNFNQTVTATGRLSSTEPNLQNIPIKYEMGREIRKVFIPNTGDSVILSADYSQIELRV EECCCCCEEEECCCCCCCCCCCCCCCEEECCCHHHHHEECCCCCCCEEEECCCCCEEEEE LAHISDDKNMISAFNEHDDIHTKTASEVFKVPIEEVTPLMRGNAKAVNFGIVYGIGDFSL EEEECCCHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEEEEECCCCC SQDLNITRKEAKQYIDAYLERYPNVKLYLENIVEEAIEKGYVSTILNRRRYIKEVKSSNK CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH IVKAAGERLAMNSPIQGSAADIIKLAMVNVHRKLKEDNFKSSIILQVHDELILNVHKDEL HHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHCCHHHH EKIKALVKKEMEQVLELKVGLDVDINIGNNWYEAK HHHHHHHHHHHHHHHHHHCCCEEEEECCCCEECCC >Mature Secondary Structure MNKERLLILDSNSLLNRAFYALPDLMTGEGIHTNAIYGFVNMLLKMKEEINPDYIVAAFD CCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEC RKAPTFRHEEYKDYKAGRKKMPEELAQQFPIVKELLSALAINIFEIDGFEADDLIGTLSV CCCCCCCCHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHEEEEECCCCHHHHHHHHHH FAEEKGIEVYIVTGDKDALQLATDNVKVVITKKGITEKEIYDKNRMVEEFGVTPKEFIDV HHCCCCCEEEEEECCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHH KGLMGDTSDNIPGVPGIGEKTAFKLIKEYKSIETVLENIENIKGKKLKENLHEYREQAIF HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH SKKLATIICNVPIDMSLEDIKSKEEYDINKVREMFERLEFKSLMNKVGKSDEEETVEEEV HHHHHHHEECCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH SYKNIVSLEEFKDLKNNIIKYKENDLYLYFELEDIVLFSKSRVKTLYANFKDEVYRIDFE HHHHHHHHHHHHHHHHHHCEEECCCEEEEEEEHHHHEECHHHHHHHHHHHCHHHHHHHHH MLIKNTKEQFIEVCKEIFESKEIKKITYDAKNPRTILRKLGIEFNGIKFDINLAAYLIDP HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHH VRKEYEISSLASEYLFKNINREDEFLKIKEVNIMPNLYKVLEEKIKEQDMEELLYKVEQP HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH LTETLSAMETEGFKVDKDILLQLEKKFKGEIESTQLEIYSMSEEEFNINSPKQLGKILFE HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH KLDLPVIKKTKTGYSTNAEVLDKLRDKHPIIDKITYYRQLTKIYSTYIEGLKAAIDEDGK HCCCCEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC IHSNFNQTVTATGRLSSTEPNLQNIPIKYEMGREIRKVFIPNTGDSVILSADYSQIELRV EECCCCCEEEECCCCCCCCCCCCCCCEEECCCHHHHHEECCCCCCCEEEECCCCCEEEEE LAHISDDKNMISAFNEHDDIHTKTASEVFKVPIEEVTPLMRGNAKAVNFGIVYGIGDFSL EEEECCCHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEEEEECCCCC SQDLNITRKEAKQYIDAYLERYPNVKLYLENIVEEAIEKGYVSTILNRRRYIKEVKSSNK CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH IVKAAGERLAMNSPIQGSAADIIKLAMVNVHRKLKEDNFKSSIILQVHDELILNVHKDEL HHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHCCHHHH EKIKALVKKEMEQVLELKVGLDVDINIGNNWYEAK HHHHHHHHHHHHHHHHHHCCCEEEEECCCCEECCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9387221; 9384377 [H]