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The map label for this gene is cafA [H]

Identifier: 148380941

GI number: 148380941

Start: 3182898

End: 3184337

Strand: Reverse

Name: cafA [H]

Synonym: CBO2990

Alternate gene names: 148380941

Gene position: 3184337-3182898 (Counterclockwise)

Preceding gene: 148380942

Following gene: 148380940

Centisome position: 81.92

GC content: 23.96

Gene sequence:

>1440_bases
GTGAAAGAAATATTTATAGAAAGGCAAAGAGAAAATGTAAGGATAGTTTTAAGAGAAAATAATCACATAAAAGAATTATT
TATAGAAGAAGATAATAAATCTCCTCAGGTTGGAGAGATTTACGTAGGTATGGTTAAAAATATAATTCCTGCTATAAAAA
GCGCTTTTGTAGATATAGGTTGGAATAAGAATGCATATCTTTATTTAGATAAAAAGTTCAATAATACCCATATAAAAAAA
GGAGATTATATTTTAGTAGAAGTAGTTAAAGAGGACTTGAATAAAAAAGGTCCTAAAGTAACTAATGCTATAACTATACC
GGGCAGATATACAGTTCTTCAAATTTTAAACAATGAAATTACATTTTCTCATAAAATAAAAGATGAAAAAATAAGAACAG
AAATACAAAATAATATAGTAAAGCCTAAAGATGTAGGAATTTTAATAAGAACGGAAGCTATAAATACAAGTATAGAAAAT
ATAAATAATGAGATAAGAAGATTATATAAAATATATAGTTCCCTTATAGAAAAATCTAAATATAAAATGAATACAGGAGT
TCTATTTCAAAACGGAGGAATAGTAGGCAAAATACTAAGAGATAAATTAAACGATAATATATCAAAAATTTATTTAAATT
GTAGAGAAGACTATGTTTATGTAAGTGAGTTTCTAAAAGAATACGGAGAAAACAATATAAAACTAGATATTTATGAAGGA
GAAAGAAATCTTTTAGATTATTATGCTATAGAAAAAGAAATACTTTCTCTTAGAAATAAAAAAGTTTATTTAAATTGTGG
AGGCTATATTATAATAGATAAAACTGAAGCAATGTATGTAGTGGACGTTAACTCTGGTAAAAATGTGAAGGGGAATTCCA
TGGAGAAAACTATTTTTACTACTAATATGGAGGCTGCGGATGAAATTTGTAATCAAATTATACTTAGAAATTTAAATGGG
ATTATTGTAATAGATTTTATAGATATGGATAATGAAAATCTTAAAGAAAAAGTCTTAGATAAATTAAAGCAGGGACTAAA
AAGAGATAAAAATAAATCTGTTGTTTATCCCTTTACTGAATTAAATTTGGTTCAAATAGCTAGAAAAAGACAGGGCAAAA
CAATATATGATTATATAGAACAGCCTTGCAAATGTTGTAGTGGAAAAGGGTCTATAGTTAGTTTTACTTATATGAAGTTA
TTAATTAGAAATGAAATAATAAATATTTTGAATATGAGAGAGATAAAGGATATATATATAGAACTAAATAAAAGATATAA
AGATTATATAGATAAAAATAGAATACAATTCATATCAGAAATTGAAGCTTTAGAAAAAAATATTTATATAAATTATATAG
ATGCTGAAAATCAGTATAAGGTAGAACCATTAATATTTAAAAATCAAATAGAAGATGTAAAAGACCTTAAGATTTATTAA

Upstream 100 bases:

>100_bases
TCCAGGGGCGTAGTCGCTCTTTGCTCCCACTGGGAGTATTAGAGCGAGTAGTCATCGGATAAATTATTTTCTTTATTTAA
AGAAGGGAAGTGCCTTTAGA

Downstream 100 bases:

>100_bases
TAAAATTTCTTTACAAAGTAAGGCCGCTATGGTAAAATGGCTTTTGTAGACCGCTCAGAAAAGGTGTTAAACATGATTTG
CATGTACCTTAATTGGCGAG

Product: ribonuclease, Rne/Rng family

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKEIFIERQRENVRIVLRENNHIKELFIEEDNKSPQVGEIYVGMVKNIIPAIKSAFVDIGWNKNAYLYLDKKFNNTHIKK
GDYILVEVVKEDLNKKGPKVTNAITIPGRYTVLQILNNEITFSHKIKDEKIRTEIQNNIVKPKDVGILIRTEAINTSIEN
INNEIRRLYKIYSSLIEKSKYKMNTGVLFQNGGIVGKILRDKLNDNISKIYLNCREDYVYVSEFLKEYGENNIKLDIYEG
ERNLLDYYAIEKEILSLRNKKVYLNCGGYIIIDKTEAMYVVDVNSGKNVKGNSMEKTIFTTNMEAADEICNQIILRNLNG
IIVIDFIDMDNENLKEKVLDKLKQGLKRDKNKSVVYPFTELNLVQIARKRQGKTIYDYIEQPCKCCSGKGSIVSFTYMKL
LIRNEIINILNMREIKDIYIELNKRYKDYIDKNRIQFISEIEALEKNIYINYIDAENQYKVEPLIFKNQIEDVKDLKIY

Sequences:

>Translated_479_residues
MKEIFIERQRENVRIVLRENNHIKELFIEEDNKSPQVGEIYVGMVKNIIPAIKSAFVDIGWNKNAYLYLDKKFNNTHIKK
GDYILVEVVKEDLNKKGPKVTNAITIPGRYTVLQILNNEITFSHKIKDEKIRTEIQNNIVKPKDVGILIRTEAINTSIEN
INNEIRRLYKIYSSLIEKSKYKMNTGVLFQNGGIVGKILRDKLNDNISKIYLNCREDYVYVSEFLKEYGENNIKLDIYEG
ERNLLDYYAIEKEILSLRNKKVYLNCGGYIIIDKTEAMYVVDVNSGKNVKGNSMEKTIFTTNMEAADEICNQIILRNLNG
IIVIDFIDMDNENLKEKVLDKLKQGLKRDKNKSVVYPFTELNLVQIARKRQGKTIYDYIEQPCKCCSGKGSIVSFTYMKL
LIRNEIINILNMREIKDIYIELNKRYKDYIDKNRIQFISEIEALEKNIYINYIDAENQYKVEPLIFKNQIEDVKDLKIY
>Mature_479_residues
MKEIFIERQRENVRIVLRENNHIKELFIEEDNKSPQVGEIYVGMVKNIIPAIKSAFVDIGWNKNAYLYLDKKFNNTHIKK
GDYILVEVVKEDLNKKGPKVTNAITIPGRYTVLQILNNEITFSHKIKDEKIRTEIQNNIVKPKDVGILIRTEAINTSIEN
INNEIRRLYKIYSSLIEKSKYKMNTGVLFQNGGIVGKILRDKLNDNISKIYLNCREDYVYVSEFLKEYGENNIKLDIYEG
ERNLLDYYAIEKEILSLRNKKVYLNCGGYIIIDKTEAMYVVDVNSGKNVKGNSMEKTIFTTNMEAADEICNQIILRNLNG
IIVIDFIDMDNENLKEKVLDKLKQGLKRDKNKSVVYPFTELNLVQIARKRQGKTIYDYIEQPCKCCSGKGSIVSFTYMKL
LIRNEIINILNMREIKDIYIELNKRYKDYIDKNRIQFISEIEALEKNIYINYIDAENQYKVEPLIFKNQIEDVKDLKIY

Specific function: Involved In The Processing Of The 5'End Of 16s Rrna. Could Be Involved In Chromosome Segregation And Cell Division. It May Be One Of The Components Of The Cytoplasmic Axial Filaments Bundles Or Merely Regulate The Formation Of This Structure. [C]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=492, Percent_Identity=28.8617886178862, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI1787325, Length=429, Percent_Identity=27.2727272727273, Blast_Score=191, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 56145; Mature: 56145

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIFIERQRENVRIVLRENNHIKELFIEEDNKSPQVGEIYVGMVKNIIPAIKSAFVDIG
CCCCEEECCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEECC
WNKNAYLYLDKKFNNTHIKKGDYILVEVVKEDLNKKGPKVTNAITIPGRYTVLQILNNEI
CCCCEEEEEECCCCCCCCCCCCEEEHHHHHHHHCCCCCCCEEEEECCCCEEEEEECCCCE
TFSHKIKDEKIRTEIQNNIVKPKDVGILIRTEAINTSIENINNEIRRLYKIYSSLIEKSK
EEEECCCHHHHHHHHHHCCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
YKMNTGVLFQNGGIVGKILRDKLNDNISKIYLNCREDYVYVSEFLKEYGENNIKLDIYEG
CEECCCEEEECCCEEHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEEC
ERNLLDYYAIEKEILSLRNKKVYLNCGGYIIIDKTEAMYVVDVNSGKNVKGNSMEKTIFT
CCCHHHHHHHHHHHHHHCCCEEEEEECCEEEEECCCEEEEEECCCCCCCCCCCCCCEEEE
TNMEAADEICNQIILRNLNGIIVIDFIDMDNENLKEKVLDKLKQGLKRDKNKSVVYPFTE
CCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECC
LNLVQIARKRQGKTIYDYIEQPCKCCSGKGSIVSFTYMKLLIRNEIINILNMREIKDIYI
CHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELNKRYKDYIDKNRIQFISEIEALEKNIYINYIDAENQYKVEPLIFKNQIEDVKDLKIY
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCEEEHHHHCCCHHCCCC
>Mature Secondary Structure
MKEIFIERQRENVRIVLRENNHIKELFIEEDNKSPQVGEIYVGMVKNIIPAIKSAFVDIG
CCCCEEECCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEECC
WNKNAYLYLDKKFNNTHIKKGDYILVEVVKEDLNKKGPKVTNAITIPGRYTVLQILNNEI
CCCCEEEEEECCCCCCCCCCCCEEEHHHHHHHHCCCCCCCEEEEECCCCEEEEEECCCCE
TFSHKIKDEKIRTEIQNNIVKPKDVGILIRTEAINTSIENINNEIRRLYKIYSSLIEKSK
EEEECCCHHHHHHHHHHCCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
YKMNTGVLFQNGGIVGKILRDKLNDNISKIYLNCREDYVYVSEFLKEYGENNIKLDIYEG
CEECCCEEEECCCEEHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEEC
ERNLLDYYAIEKEILSLRNKKVYLNCGGYIIIDKTEAMYVVDVNSGKNVKGNSMEKTIFT
CCCHHHHHHHHHHHHHHCCCEEEEEECCEEEEECCCEEEEEECCCCCCCCCCCCCCEEEE
TNMEAADEICNQIILRNLNGIIVIDFIDMDNENLKEKVLDKLKQGLKRDKNKSVVYPFTE
CCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECC
LNLVQIARKRQGKTIYDYIEQPCKCCSGKGSIVSFTYMKLLIRNEIINILNMREIKDIYI
CHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELNKRYKDYIDKNRIQFISEIEALEKNIYINYIDAENQYKVEPLIFKNQIEDVKDLKIY
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCEEEHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]