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The map label for this gene is purF [H]

Identifier: 148380867

GI number: 148380867

Start: 3103718

End: 3105100

Strand: Reverse

Name: purF [H]

Synonym: CBO2913

Alternate gene names: 148380867

Gene position: 3105100-3103718 (Counterclockwise)

Preceding gene: 148380868

Following gene: 148380866

Centisome position: 79.89

GC content: 28.13

Gene sequence:

>1383_bases
ATGCAAATGTTATTTGATAGAAAGCAGGATAAATTTAGGGAAGAATGTGGAGTTTTTGGCGTTTTTAAAGATTATACATC
AGAACTTGGAGAAATATTTTATCCAGGGTTAGTATCTCTTCAACATAGAGGAGAAGAAAGTTCAGGAATAAGCTATACAA
CTAGCAAAGGTATGATAACTAAAAAAGCTCTTGGAATGGTGTCTAACTTGTTTTCTAAAGAGGATTTTTATAAAATGAAA
TATTTTTCTGCTATAGGACATGTTAGATATTCAACTAGTGGTAATGCTTCTATTGAAAATGCACAACCATTTCAAGAAGA
AACTATAGATGGATCTATTTCTTTAGCTCATAATGGAAATTTATTAAATTATTTAAATATTAAGTATGAATTAGAGAAAA
AAGGAACGATATTTAAAAGTAATTCAGACTCTGAAATTATACTAAAGTTTATATTAGAAAAAATTCAAGAAGTAAGAGAA
ATAGAAAAAGCCATAGCTTATGCTATTAATACTTTAAAGGGAGCTTTTTCAGTACTTATTTTAACCGAAGATAAGTTAAT
AGGTTTTAGAGATAAAAATGGTATAAGACCTTTATGTCTAGGAAAAATAGAAGGCAATTATATTTTATCTTCAGAAAGTA
CTTCCATAGATGTTGTGGGAGGAGAATACATAAGAGATGTAGATCCAGGGGAAATAGTGGTTATAAATAAAAAAGGAATA
AAATTTATAAAGAATAAAGAAGTTTATTGTGGTTCTCTCTGCGCATTAGAATATATATATTTTTCACGACCAGATAGTAT
CATAGATGGGATAAATCTATCACAATTTAGAATTAAATGTGGAGAAAAGTTATATGAAAAATACAAATCAAACTCAGATA
TTGTAATGGGAGTACCTGAATCGGGAAATTTTGCAGCTTTAGGTTATTCTAAGGAATCAAATATACCCTATAGTATAGGT
CTTATTAAAAATTCTTATGTAGGGAGAAATTTTATAAAAGCTACAGAAAAGGAAAGAAAAAGGGATATAAATATAAAAAT
AAATGCCATAAAAAGTATAGTTCAAGATAAAAGCATTATTGTGATAGATGATTCTATTGTAAGAGGCACTAGTAGTAAAA
AGGTAGTGTCCGCATTAAAAAAAGCAGGGGCAAGAGAGGTTCACTTTATGGTGGCATCTCCTAAAATAAATTATTATTGT
AATTTGGGTATAGATATAAAAAGTAAAAAAGAGCTTCTATCTTTCCAAAAAACAAAGGAAGAAATGAGAAAGTTTATAGG
GGCAGATAGCTTAGAATTTTTAAGTTTAATAGATATGGAACAGTGTTTAAATAAATCTAACATTTGTACAGGTTGTTTTG
ATGGAAGTTATGCTAATTATTAA

Upstream 100 bases:

>100_bases
AGTATATATAGTAAATTATATGTAGTATAAAAAGCTTACCTTCGGGTGGGCTTTATTTTTTTATTTTTAAATTATAATTT
GATGTTTTAAGTTAGAAGGG

Downstream 100 bases:

>100_bases
ATTTCATAGCTAGTATAGTATACATAAAAATTTATAATATAAAATAAATTTAATTAGTTTTTAACATTGATTTCTTTTTA
CTTTCTAGAGGTAATATTTT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MQMLFDRKQDKFREECGVFGVFKDYTSELGEIFYPGLVSLQHRGEESSGISYTTSKGMITKKALGMVSNLFSKEDFYKMK
YFSAIGHVRYSTSGNASIENAQPFQEETIDGSISLAHNGNLLNYLNIKYELEKKGTIFKSNSDSEIILKFILEKIQEVRE
IEKAIAYAINTLKGAFSVLILTEDKLIGFRDKNGIRPLCLGKIEGNYILSSESTSIDVVGGEYIRDVDPGEIVVINKKGI
KFIKNKEVYCGSLCALEYIYFSRPDSIIDGINLSQFRIKCGEKLYEKYKSNSDIVMGVPESGNFAALGYSKESNIPYSIG
LIKNSYVGRNFIKATEKERKRDINIKINAIKSIVQDKSIIVIDDSIVRGTSSKKVVSALKKAGAREVHFMVASPKINYYC
NLGIDIKSKKELLSFQKTKEEMRKFIGADSLEFLSLIDMEQCLNKSNICTGCFDGSYANY

Sequences:

>Translated_460_residues
MQMLFDRKQDKFREECGVFGVFKDYTSELGEIFYPGLVSLQHRGEESSGISYTTSKGMITKKALGMVSNLFSKEDFYKMK
YFSAIGHVRYSTSGNASIENAQPFQEETIDGSISLAHNGNLLNYLNIKYELEKKGTIFKSNSDSEIILKFILEKIQEVRE
IEKAIAYAINTLKGAFSVLILTEDKLIGFRDKNGIRPLCLGKIEGNYILSSESTSIDVVGGEYIRDVDPGEIVVINKKGI
KFIKNKEVYCGSLCALEYIYFSRPDSIIDGINLSQFRIKCGEKLYEKYKSNSDIVMGVPESGNFAALGYSKESNIPYSIG
LIKNSYVGRNFIKATEKERKRDINIKINAIKSIVQDKSIIVIDDSIVRGTSSKKVVSALKKAGAREVHFMVASPKINYYC
NLGIDIKSKKELLSFQKTKEEMRKFIGADSLEFLSLIDMEQCLNKSNICTGCFDGSYANY
>Mature_460_residues
MQMLFDRKQDKFREECGVFGVFKDYTSELGEIFYPGLVSLQHRGEESSGISYTTSKGMITKKALGMVSNLFSKEDFYKMK
YFSAIGHVRYSTSGNASIENAQPFQEETIDGSISLAHNGNLLNYLNIKYELEKKGTIFKSNSDSEIILKFILEKIQEVRE
IEKAIAYAINTLKGAFSVLILTEDKLIGFRDKNGIRPLCLGKIEGNYILSSESTSIDVVGGEYIRDVDPGEIVVINKKGI
KFIKNKEVYCGSLCALEYIYFSRPDSIIDGINLSQFRIKCGEKLYEKYKSNSDIVMGVPESGNFAALGYSKESNIPYSIG
LIKNSYVGRNFIKATEKERKRDINIKINAIKSIVQDKSIIVIDDSIVRGTSSKKVVSALKKAGAREVHFMVASPKINYYC
NLGIDIKSKKELLSFQKTKEEMRKFIGADSLEFLSLIDMEQCLNKSNICTGCFDGSYANY

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=507, Percent_Identity=34.1222879684418, Blast_Score=294, Evalue=1e-79,
Organism=Escherichia coli, GI1788651, Length=465, Percent_Identity=35.2688172043011, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1790167, Length=229, Percent_Identity=26.6375545851528, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17554892, Length=477, Percent_Identity=35.2201257861635, Blast_Score=271, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI17539970, Length=188, Percent_Identity=28.7234042553192, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323958, Length=470, Percent_Identity=34.2553191489362, Blast_Score=251, Evalue=1e-67,
Organism=Drosophila melanogaster, GI28573187, Length=487, Percent_Identity=35.523613963039, Blast_Score=321, Evalue=8e-88,
Organism=Drosophila melanogaster, GI24659598, Length=483, Percent_Identity=35.4037267080745, Blast_Score=314, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24659604, Length=457, Percent_Identity=35.2297592997812, Blast_Score=305, Evalue=6e-83,
Organism=Drosophila melanogaster, GI21357745, Length=182, Percent_Identity=29.1208791208791, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51707; Mature: 51707

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMLFDRKQDKFREECGVFGVFKDYTSELGEIFYPGLVSLQHRGEESSGISYTTSKGMIT
CCCHHHCHHHHHHHHCCEEEEHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEECCCCCHH
KKALGMVSNLFSKEDFYKMKYFSAIGHVRYSTSGNASIENAQPFQEETIDGSISLAHNGN
HHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHCCCCEEEEEECCC
LLNYLNIKYELEKKGTIFKSNSDSEIILKFILEKIQEVREIEKAIAYAINTLKGAFSVLI
EEEEEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
LTEDKLIGFRDKNGIRPLCLGKIEGNYILSSESTSIDVVGGEYIRDVDPGEIVVINKKGI
EECCCEEEEECCCCCCCEEEEEECCCEEEECCCCEEEEECCHHHCCCCCCCEEEEECCCC
KFIKNKEVYCGSLCALEYIYFSRPDSIIDGINLSQFRIKCGEKLYEKYKSNSDIVMGVPE
EEEECCCCEECHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCC
SGNFAALGYSKESNIPYSIGLIKNSYVGRNFIKATEKERKRDINIKINAIKSIVQDKSII
CCCEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHCCCCEE
VIDDSIVRGTSSKKVVSALKKAGAREVHFMVASPKINYYCNLGIDIKSKKELLSFQKTKE
EEECHHCCCCCHHHHHHHHHHCCCCEEEEEEECCCEEEEEEECEEECCHHHHHHHHHHHH
EMRKFIGADSLEFLSLIDMEQCLNKSNICTGCFDGSYANY
HHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MQMLFDRKQDKFREECGVFGVFKDYTSELGEIFYPGLVSLQHRGEESSGISYTTSKGMIT
CCCHHHCHHHHHHHHCCEEEEHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEECCCCCHH
KKALGMVSNLFSKEDFYKMKYFSAIGHVRYSTSGNASIENAQPFQEETIDGSISLAHNGN
HHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHCCCCEEEEEECCC
LLNYLNIKYELEKKGTIFKSNSDSEIILKFILEKIQEVREIEKAIAYAINTLKGAFSVLI
EEEEEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
LTEDKLIGFRDKNGIRPLCLGKIEGNYILSSESTSIDVVGGEYIRDVDPGEIVVINKKGI
EECCCEEEEECCCCCCCEEEEEECCCEEEECCCCEEEEECCHHHCCCCCCCEEEEECCCC
KFIKNKEVYCGSLCALEYIYFSRPDSIIDGINLSQFRIKCGEKLYEKYKSNSDIVMGVPE
EEEECCCCEECHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCC
SGNFAALGYSKESNIPYSIGLIKNSYVGRNFIKATEKERKRDINIKINAIKSIVQDKSII
CCCEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHCCCCEE
VIDDSIVRGTSSKKVVSALKKAGAREVHFMVASPKINYYCNLGIDIKSKKELLSFQKTKE
EEECHHCCCCCHHHHHHHHHHCCCCEEEEEEECCCEEEEEEECEEECCHHHHHHHHHHHH
EMRKFIGADSLEFLSLIDMEQCLNKSNICTGCFDGSYANY
HHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8809759 [H]