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The map label for this gene is thiC

Identifier: 148380866

GI number: 148380866

Start: 3102194

End: 3103507

Strand: Reverse

Name: thiC

Synonym: CBO2911

Alternate gene names: 148380866

Gene position: 3103507-3102194 (Counterclockwise)

Preceding gene: 148380867

Following gene: 148380865

Centisome position: 79.84

GC content: 33.03

Gene sequence:

>1314_bases
ATGAATTATACAACTCAAATGGATGCTGCTAAAAAAGGAATAGTTACAAAAGAGATGGAAATAGTAGCTAAAAAGGAAAA
TATGAATGTGAAAGACCTTATGGAATTGGTATCTAAAGGTAAGGTTGCTATACCTGCTAATAAAAATCATAAATCTTTAG
ATCCAGAAGGAATAGGTCAAGGTTTAAGAACTAAAATAAATGTCAATTTAGGAATATCTAAAGATTGCTATAATATAGAT
ATGGAATTAGAAAAAGTACAAAAAGCAATAGATATGAAGGCAGAGGCTATAATGGATTTGAGCTGCTTTGGAAAAACAGA
AGAATTTAGAAAAAGATTGATAGACATGTCACCTGCCATAATAGGTACTGTTCCTATATATGATGCAGTAGGTTTCTATG
ATAAAGAATTAAAAGATATTACATCAGAAGAATTTTTAAAGGTAGCAGAGAAGCATGCAGAGAATGGAGCAGACTTTTTA
ACAATACATGTAGGTATGAATAGAAAAACTGCAGCTACATTTAAGAAAAATCCAAGAAGAATGAACATAGTTTCAAGAGG
AGGATCCCTTTTATATGCATGGATGGAACTAAATAATAAAGAAAATCCATTCTATGAAGGATTTGATAAACTTTTAGATA
TATGTGAAAAGTATGATGTTACATTAAGCTTAGGAGATGCTTGTCGTCCAGGTTGTATAGAGGATTCTACAGATGCAAGC
CAGATAGAAGAACTTATAGCTTTAGGAGAACTTACTAAAAGAGCCTGGGAGAGGAATGTACAAGTTATAATAGAGGGACC
AGGACACATGACTTTAGATGAAATAGAAACAAATATGAAAATAGAGAAAAAATTATGTCATGGAGCTCCGTTCTATGTTT
TAGGACCAATAGTTACAGATATAGCTCCAGGTTATGATCACATAACTTCCGCTATAGGTGGTGCTATTGCAGCAACTCAT
GGTGCAGATTTCTTATGCTATGTAACTCCAGCAGAACATTTAAGATTACCAAACCTAGATGATATGAAGGAAGGTATAAT
AGCATCAAAGATAGCAGCTCATGCAGCAGATTTAGCTAAAGGAGTAAAAGGAGCAAGAGATTGGGATAATGCTATGGCTA
AAGCTAGAAGAGATTTAGACTGGGAAAGAATGTTTGAGTTATCTATAGATGAAGAAAAAGCTAGAAGATATAGAGAAGAA
TCTAAGGCAAAAAGTAAAGATTCTTGTACAATGTGCGGAAAAATGTGTGCAGTAAGAAATATGAACAGAGTTACAGAAGG
AAAAGACTTAAACATGTTAAGAGATGATGATTAA

Upstream 100 bases:

>100_bases
TCATTATATTGCAATAAACTTAAAGAAATTAATGTACATATAAATAAATAAAATCCCTGTAGCATATTTTACAGGAGATA
TGAAAAGGAGAAATGTTATT

Downstream 100 bases:

>100_bases
TGTATAGGAGTGACTATAATGGCAAATATAAATTTGAGTTTACAAGTGCTACCCGTAGTTAGTGAAGAAGAGATATATCC
AGTAGTGGATAAAGTTATAG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MNYTTQMDAAKKGIVTKEMEIVAKKENMNVKDLMELVSKGKVAIPANKNHKSLDPEGIGQGLRTKINVNLGISKDCYNID
MELEKVQKAIDMKAEAIMDLSCFGKTEEFRKRLIDMSPAIIGTVPIYDAVGFYDKELKDITSEEFLKVAEKHAENGADFL
TIHVGMNRKTAATFKKNPRRMNIVSRGGSLLYAWMELNNKENPFYEGFDKLLDICEKYDVTLSLGDACRPGCIEDSTDAS
QIEELIALGELTKRAWERNVQVIIEGPGHMTLDEIETNMKIEKKLCHGAPFYVLGPIVTDIAPGYDHITSAIGGAIAATH
GADFLCYVTPAEHLRLPNLDDMKEGIIASKIAAHAADLAKGVKGARDWDNAMAKARRDLDWERMFELSIDEEKARRYREE
SKAKSKDSCTMCGKMCAVRNMNRVTEGKDLNMLRDDD

Sequences:

>Translated_437_residues
MNYTTQMDAAKKGIVTKEMEIVAKKENMNVKDLMELVSKGKVAIPANKNHKSLDPEGIGQGLRTKINVNLGISKDCYNID
MELEKVQKAIDMKAEAIMDLSCFGKTEEFRKRLIDMSPAIIGTVPIYDAVGFYDKELKDITSEEFLKVAEKHAENGADFL
TIHVGMNRKTAATFKKNPRRMNIVSRGGSLLYAWMELNNKENPFYEGFDKLLDICEKYDVTLSLGDACRPGCIEDSTDAS
QIEELIALGELTKRAWERNVQVIIEGPGHMTLDEIETNMKIEKKLCHGAPFYVLGPIVTDIAPGYDHITSAIGGAIAATH
GADFLCYVTPAEHLRLPNLDDMKEGIIASKIAAHAADLAKGVKGARDWDNAMAKARRDLDWERMFELSIDEEKARRYREE
SKAKSKDSCTMCGKMCAVRNMNRVTEGKDLNMLRDDD
>Mature_437_residues
MNYTTQMDAAKKGIVTKEMEIVAKKENMNVKDLMELVSKGKVAIPANKNHKSLDPEGIGQGLRTKINVNLGISKDCYNID
MELEKVQKAIDMKAEAIMDLSCFGKTEEFRKRLIDMSPAIIGTVPIYDAVGFYDKELKDITSEEFLKVAEKHAENGADFL
TIHVGMNRKTAATFKKNPRRMNIVSRGGSLLYAWMELNNKENPFYEGFDKLLDICEKYDVTLSLGDACRPGCIEDSTDAS
QIEELIALGELTKRAWERNVQVIIEGPGHMTLDEIETNMKIEKKLCHGAPFYVLGPIVTDIAPGYDHITSAIGGAIAATH
GADFLCYVTPAEHLRLPNLDDMKEGIIASKIAAHAADLAKGVKGARDWDNAMAKARRDLDWERMFELSIDEEKARRYREE
SKAKSKDSCTMCGKMCAVRNMNRVTEGKDLNMLRDDD

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=447, Percent_Identity=43.4004474272931, Blast_Score=359, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_CLOB1 (A7FXG9)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385173.1
- STRING:   A7FXG9
- GeneID:   5396273
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2874
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- ProtClustDB:   PRK13352
- BioCyc:   CBOT441770:CLB_2874-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 48860; Mature: 48860

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: BINDING 69-69 BINDING 98-98 BINDING 127-127 BINDING 163-163 BINDING 265-265 BINDING 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTTQMDAAKKGIVTKEMEIVAKKENMNVKDLMELVSKGKVAIPANKNHKSLDPEGIGQ
CCCCCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCCCCCCHHHHCC
GLRTKINVNLGISKDCYNIDMELEKVQKAIDMKAEAIMDLSCFGKTEEFRKRLIDMSPAI
CCCEEEEEEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHH
IGTVPIYDAVGFYDKELKDITSEEFLKVAEKHAENGADFLTIHVGMNRKTAATFKKNPRR
EECCHHHHHHCHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCE
MNIVSRGGSLLYAWMELNNKENPFYEGFDKLLDICEKYDVTLSLGDACRPGCIEDSTDAS
EEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHH
QIEELIALGELTKRAWERNVQVIIEGPGHMTLDEIETNMKIEKKLCHGAPFYVLGPIVTD
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCHHHHHHHHCCCCHHHHHHHHHH
IAPGYDHITSAIGGAIAATHGADFLCYVTPAEHLRLPNLDDMKEGIIASKIAAHAADLAK
CCCCHHHHHHHHCCHHEECCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
GVKGARDWDNAMAKARRDLDWERMFELSIDEEKARRYREESKAKSKDSCTMCGKMCAVRN
HCCCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
MNRVTEGKDLNMLRDDD
CHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNYTTQMDAAKKGIVTKEMEIVAKKENMNVKDLMELVSKGKVAIPANKNHKSLDPEGIGQ
CCCCCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCCCCCCHHHHCC
GLRTKINVNLGISKDCYNIDMELEKVQKAIDMKAEAIMDLSCFGKTEEFRKRLIDMSPAI
CCCEEEEEEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHH
IGTVPIYDAVGFYDKELKDITSEEFLKVAEKHAENGADFLTIHVGMNRKTAATFKKNPRR
EECCHHHHHHCHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCE
MNIVSRGGSLLYAWMELNNKENPFYEGFDKLLDICEKYDVTLSLGDACRPGCIEDSTDAS
EEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHH
QIEELIALGELTKRAWERNVQVIIEGPGHMTLDEIETNMKIEKKLCHGAPFYVLGPIVTD
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCHHHHHHHHCCCCHHHHHHHHHH
IAPGYDHITSAIGGAIAATHGADFLCYVTPAEHLRLPNLDDMKEGIIASKIAAHAADLAK
CCCCHHHHHHHHCCHHEECCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
GVKGARDWDNAMAKARRDLDWERMFELSIDEEKARRYREESKAKSKDSCTMCGKMCAVRN
HCCCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
MNRVTEGKDLNMLRDDD
CHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA