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The map label for this gene is gptB [H]

Identifier: 148380802

GI number: 148380802

Start: 3022098

End: 3023009

Strand: Direct

Name: gptB [H]

Synonym: CBO2845

Alternate gene names: 148380802

Gene position: 3022098-3023009 (Clockwise)

Preceding gene: 148380798

Following gene: 148380803

Centisome position: 77.75

GC content: 35.09

Gene sequence:

>912_bases
ATGGCAAAAGAATTAAAATTAACCAAAAAAGATCATATTTCTGTTTGGTTGCGTTCATTTTTCCTTCAAGGTTCTTGGAA
CTATGAAAGAATGCAAAACGGTGGTTGGGCATTCGCAATGATTCCTGCAATCAAAAAATTATACAAGACTAAAGAAGATC
GAGCAGATGCATTGAAACGTCACTTGGAGTTCTTTAACACTCACCCATATGTAGCTTCACCAGTTCTTGGGGTAACATTA
GCTTTAGAAGAACAACGTGCAAATGGTGCAGAAATCGATGATATAACTATTCAAGGTGTTAAAGTTGGTATGATGGGACC
TTTAGCTGGTATTGGAGATCCAGTTTTTTGGTTTACTGTTAGGTCAATTTTAGGGGCATTAGCTTCTTCACTTGCTATGA
ATGGTAATATCCTAGGACCATTAATCTTTTTCTTGGCTTGGAATATCATCCGTATGGGATTAATGTGGTATACACAAGAG
TTCGGTTTCAAAACAGAATCTTGTATTGCTGATGATTTATCAGGTGGTTTACTACAAGATATTACAAAAGGAGCATCCAT
CCTTGGTATGTTCATTTTAGGAGCATTAGTTAACAGATGGGTATCCGTTAAATTTACACCAGTAGTATCATCCGTTAAGT
TAAGTGATGGTGCTTTCATTGATTGGAGCAAACTTCCTAAAGGAGTGAAAGGTATTCAACAAGTTCTTTTACAACAAGGA
TCTGGTATGTCATTAACTGATTATAAAGTTACAACATTCCAAGATAGCTTGGATTCATTAATTCCTGGACTTGCAGAATT
AATAATTACATTTATTTGTATGTGGTTACTTAAGAAGAAAGTATCTCCAATTACCATTATCCTTGGGATATTTGCAATGG
GTATTGTTTTCCACTTAATCGGTTTAATGTAA

Upstream 100 bases:

>100_bases
AAAGTATTTTCTGCTGTTACTTGTGGTGTTGTTTTCTTGTAATTCTTTTTCTTCTTACGACAAACAGATTTTAAATTCAG
AATTGACAAAGGACATGAAA

Downstream 100 bases:

>100_bases
CATTTTTTATTTAGCCTAGGCTTTTAGCCTGGGCTTTTGTTAGCATATAAAGCAGTTAAAGAATGAAACAAATTGATGAA
ATATGTGTTATTTTTAATGG

Product: PTS system, mannose-specific IID component

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MAKELKLTKKDHISVWLRSFFLQGSWNYERMQNGGWAFAMIPAIKKLYKTKEDRADALKRHLEFFNTHPYVASPVLGVTL
ALEEQRANGAEIDDITIQGVKVGMMGPLAGIGDPVFWFTVRSILGALASSLAMNGNILGPLIFFLAWNIIRMGLMWYTQE
FGFKTESCIADDLSGGLLQDITKGASILGMFILGALVNRWVSVKFTPVVSSVKLSDGAFIDWSKLPKGVKGIQQVLLQQG
SGMSLTDYKVTTFQDSLDSLIPGLAELIITFICMWLLKKKVSPITIILGIFAMGIVFHLIGLM

Sequences:

>Translated_303_residues
MAKELKLTKKDHISVWLRSFFLQGSWNYERMQNGGWAFAMIPAIKKLYKTKEDRADALKRHLEFFNTHPYVASPVLGVTL
ALEEQRANGAEIDDITIQGVKVGMMGPLAGIGDPVFWFTVRSILGALASSLAMNGNILGPLIFFLAWNIIRMGLMWYTQE
FGFKTESCIADDLSGGLLQDITKGASILGMFILGALVNRWVSVKFTPVVSSVKLSDGAFIDWSKLPKGVKGIQQVLLQQG
SGMSLTDYKVTTFQDSLDSLIPGLAELIITFICMWLLKKKVSPITIILGIFAMGIVFHLIGLM
>Mature_302_residues
AKELKLTKKDHISVWLRSFFLQGSWNYERMQNGGWAFAMIPAIKKLYKTKEDRADALKRHLEFFNTHPYVASPVLGVTLA
LEEQRANGAEIDDITIQGVKVGMMGPLAGIGDPVFWFTVRSILGALASSLAMNGNILGPLIFFLAWNIIRMGLMWYTQEF
GFKTESCIADDLSGGLLQDITKGASILGMFILGALVNRWVSVKFTPVVSSVKLSDGAFIDWSKLPKGVKGIQQVLLQQGS
GMSLTDYKVTTFQDSLDSLIPGLAELIITFICMWLLKKKVSPITIILGIFAMGIVFHLIGLM

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=301, Percent_Identity=49.5016611295681, Blast_Score=300, Evalue=8e-83,
Organism=Escherichia coli, GI1789529, Length=303, Percent_Identity=32.3432343234323, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 33514; Mature: 33383

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKELKLTKKDHISVWLRSFFLQGSWNYERMQNGGWAFAMIPAIKKLYKTKEDRADALKR
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEHHHHHHHHHHHCCHHHHHHHHH
HLEFFNTHPYVASPVLGVTLALEEQRANGAEIDDITIQGVKVGMMGPLAGIGDPVFWFTV
HHHHHCCCCCHHCHHHHHHEEEEHHCCCCCCCCCEEECCEEECCCCCCCCCCCHHHHHHH
RSILGALASSLAMNGNILGPLIFFLAWNIIRMGLMWYTQEFGFKTESCIADDLSGGLLQD
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHH
ITKGASILGMFILGALVNRWVSVKFTPVVSSVKLSDGAFIDWSKLPKGVKGIQQVLLQQG
HHHHHHHHHHHHHHHHHHHHEEEEEEHHHHEEEECCCCEEEHHHCCHHHHHHHHHHHHCC
SGMSLTDYKVTTFQDSLDSLIPGLAELIITFICMWLLKKKVSPITIILGIFAMGIVFHLI
CCCCEECEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GLM
HCC
>Mature Secondary Structure 
AKELKLTKKDHISVWLRSFFLQGSWNYERMQNGGWAFAMIPAIKKLYKTKEDRADALKR
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCEEEHHHHHHHHHHHCCHHHHHHHHH
HLEFFNTHPYVASPVLGVTLALEEQRANGAEIDDITIQGVKVGMMGPLAGIGDPVFWFTV
HHHHHCCCCCHHCHHHHHHEEEEHHCCCCCCCCCEEECCEEECCCCCCCCCCCHHHHHHH
RSILGALASSLAMNGNILGPLIFFLAWNIIRMGLMWYTQEFGFKTESCIADDLSGGLLQD
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHH
ITKGASILGMFILGALVNRWVSVKFTPVVSSVKLSDGAFIDWSKLPKGVKGIQQVLLQQG
HHHHHHHHHHHHHHHHHHHHEEEEEEHHHHEEEECCCCEEEHHHCCHHHHHHHHHHHHCC
SGMSLTDYKVTTFQDSLDSLIPGLAELIITFICMWLLKKKVSPITIILGIFAMGIVFHLI
CCCCEECEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GLM
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]