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The map label for this gene is cisA [H]

Identifier: 148380349

GI number: 148380349

Start: 2521384

End: 2523003

Strand: Direct

Name: cisA [H]

Synonym: CBO2394

Alternate gene names: 148380349

Gene position: 2521384-2523003 (Clockwise)

Preceding gene: 148380348

Following gene: 148380351

Centisome position: 64.87

GC content: 28.77

Gene sequence:

>1620_bases
ATGAAGGCAGCAATTTACAGCAGAAAATCAATGTTCACAGGTAAAGGTGACTCTGTAGAAAATCAAGTCCAAATGTGTAA
AGATTATGGAAACAGATTAAATGTAGATGAATATTTAATATATGAGGATGAAGGATACTCAGGTGGTAATATTAATAGAC
CTAAATTTCAAGAATTATTACAAGATGTTAAAAAGAAAAAGTTTGATGTGCTTATATGCTATAGGCTAGATAGAATTTCA
AGAAATGTAGCAGACTTCTCTTCTACTCTTGAACTACTTCAAGACAATAATATAAGTTTTGTAAGCATAAAAGAACAGTT
TGACACTTCTACGCCAATGGGTAAAGCTATGGTTTATATAGCTTCTGTTTTTGCACAGTTAGAACGTGAAACCATTGCTG
AACGTGTAAGAGATAATATGCTAGAACTTGCTAAATCCGGAAGATGGTTAGGCGGCCAAACTCCTCTAGGGTTTGAATCT
GAAAAAATATCTTATTTTGATGCAGAAATGAAAGAAAGAACTATGTATAAACTTTCTCCTGAATCTAAAGAATTAGAATT
AGTTAAATTAATTTATAATAAATACTTAGAAACTGGATCTATACACCTAACATTAAAATATTTATTATCTAATAATATAA
AAGGTAAAAATGGTGGAGAATTTGCATCCATGTCTATAAATGATATATTAAGGAATCCTGTATATGCTAAATCTAATGAA
ATGGTGCTTAATTATCTTAAAGATAAAGGTATGAATGTATGTGGTACTGCTAATGGTAATGGTATCTTGATATACAATAA
AAGAAATTCTAAATATAAGAGGAAAGATATAAATGAATGGGTAGCTGCTGTTAGTAAACACAAAGGCATTATACCTGCTA
ATGTATGGATAGAAGTTCAAAAAACTCTAGATAAAAATTCTAAAAAAGATACTCCAAGACAAGGAACATCTAAAAAGGCA
TTATTATCTGGAGTTTTAAAATGTAGTAGATGCGGTGCTCCTATGCGAGTTACTTATGGTAGGAAAAGAAAAGATGGCAC
TTCCATATATTATTATACATGTACCATGAAAGCTCATAGTGGCAAAACTAGGTGTGATAATCCTAATGTACGCGGGGACT
ATTTAGAAAAGAACATTATTACAGAATTAAAGAACTTAAATTCTGATGTAGTCATTAAAGGGCTTGAAGAACATAAAAAA
CAACTAGTTGCAACAACAGAAAATTCAATTATAAAAAATATTTCTAAAGAAATAGAAGAAAAAAAGAAGGAAATGGATTC
TTTGATTAATAAACTTTCTAAGATAGAAAGTGATATAGCATCTGAATTTATATTATCTAAAGTAGATTCTTTAGGAAAAG
AAATTAAAGAATTGGAAGTTAATTTATTAAAAAGAACCGATAAGAGAAAAGAAAACTCTGATATTGAACTTAATATTGAA
ATAGTGTTGCAAGCAATAAAAGAATTTAATGATTTTTTCAATAGTGTTGAGAGTTTTAAAGATGATGAATTAACTATACA
AAGGAAAAGATATCTTCTTGAAAGAGCTGTAGATGAAATTAGAATAGATGGAGAAACTAATGGAATAGATATTGATTTAT
GGAGGGCTAAAAAAAAATAA

Upstream 100 bases:

>100_bases
CAGAAGAATTAGATGTTTGTCCTCGACTACTTATACGTTGTACAATAGAATGTAGTGAATGTAAAAAACAATACAAATGT
ATTTGTCAGGGGGAATACTA

Downstream 100 bases:

>100_bases
CCCCCTCTATGGAATATGATGCCAAAATGTTGCAATTACATAAAGCATGTACACGCATGGCTTTATGATGTAAATAAAAT
AGAACCTGCTGTAAAAAATC

Product: resolvase

Products: NA

Alternate protein names: Stage IV sporulation protein CA [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MKAAIYSRKSMFTGKGDSVENQVQMCKDYGNRLNVDEYLIYEDEGYSGGNINRPKFQELLQDVKKKKFDVLICYRLDRIS
RNVADFSSTLELLQDNNISFVSIKEQFDTSTPMGKAMVYIASVFAQLERETIAERVRDNMLELAKSGRWLGGQTPLGFES
EKISYFDAEMKERTMYKLSPESKELELVKLIYNKYLETGSIHLTLKYLLSNNIKGKNGGEFASMSINDILRNPVYAKSNE
MVLNYLKDKGMNVCGTANGNGILIYNKRNSKYKRKDINEWVAAVSKHKGIIPANVWIEVQKTLDKNSKKDTPRQGTSKKA
LLSGVLKCSRCGAPMRVTYGRKRKDGTSIYYYTCTMKAHSGKTRCDNPNVRGDYLEKNIITELKNLNSDVVIKGLEEHKK
QLVATTENSIIKNISKEIEEKKKEMDSLINKLSKIESDIASEFILSKVDSLGKEIKELEVNLLKRTDKRKENSDIELNIE
IVLQAIKEFNDFFNSVESFKDDELTIQRKRYLLERAVDEIRIDGETNGIDIDLWRAKKK

Sequences:

>Translated_539_residues
MKAAIYSRKSMFTGKGDSVENQVQMCKDYGNRLNVDEYLIYEDEGYSGGNINRPKFQELLQDVKKKKFDVLICYRLDRIS
RNVADFSSTLELLQDNNISFVSIKEQFDTSTPMGKAMVYIASVFAQLERETIAERVRDNMLELAKSGRWLGGQTPLGFES
EKISYFDAEMKERTMYKLSPESKELELVKLIYNKYLETGSIHLTLKYLLSNNIKGKNGGEFASMSINDILRNPVYAKSNE
MVLNYLKDKGMNVCGTANGNGILIYNKRNSKYKRKDINEWVAAVSKHKGIIPANVWIEVQKTLDKNSKKDTPRQGTSKKA
LLSGVLKCSRCGAPMRVTYGRKRKDGTSIYYYTCTMKAHSGKTRCDNPNVRGDYLEKNIITELKNLNSDVVIKGLEEHKK
QLVATTENSIIKNISKEIEEKKKEMDSLINKLSKIESDIASEFILSKVDSLGKEIKELEVNLLKRTDKRKENSDIELNIE
IVLQAIKEFNDFFNSVESFKDDELTIQRKRYLLERAVDEIRIDGETNGIDIDLWRAKKK
>Mature_539_residues
MKAAIYSRKSMFTGKGDSVENQVQMCKDYGNRLNVDEYLIYEDEGYSGGNINRPKFQELLQDVKKKKFDVLICYRLDRIS
RNVADFSSTLELLQDNNISFVSIKEQFDTSTPMGKAMVYIASVFAQLERETIAERVRDNMLELAKSGRWLGGQTPLGFES
EKISYFDAEMKERTMYKLSPESKELELVKLIYNKYLETGSIHLTLKYLLSNNIKGKNGGEFASMSINDILRNPVYAKSNE
MVLNYLKDKGMNVCGTANGNGILIYNKRNSKYKRKDINEWVAAVSKHKGIIPANVWIEVQKTLDKNSKKDTPRQGTSKKA
LLSGVLKCSRCGAPMRVTYGRKRKDGTSIYYYTCTMKAHSGKTRCDNPNVRGDYLEKNIITELKNLNSDVVIKGLEEHKK
QLVATTENSIIKNISKEIEEKKKEMDSLINKLSKIESDIASEFILSKVDSLGKEIKELEVNLLKRTDKRKENSDIELNIE
IVLQAIKEFNDFFNSVESFKDDELTIQRKRYLLERAVDEIRIDGETNGIDIDLWRAKKK

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=117, Percent_Identity=35.042735042735, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 61786; Mature: 61786

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAAIYSRKSMFTGKGDSVENQVQMCKDYGNRLNVDEYLIYEDEGYSGGNINRPKFQELL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHH
QDVKKKKFDVLICYRLDRISRNVADFSSTLELLQDNNISFVSIKEQFDTSTPMGKAMVYI
HHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCHHHHHHHH
ASVFAQLERETIAERVRDNMLELAKSGRWLGGQTPLGFESEKISYFDAEMKERTMYKLSP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEECC
ESKELELVKLIYNKYLETGSIHLTLKYLLSNNIKGKNGGEFASMSINDILRNPVYAKSNE
CCHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCH
MVLNYLKDKGMNVCGTANGNGILIYNKRNSKYKRKDINEWVAAVSKHKGIIPANVWIEVQ
HHHHHHHHCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHEEEEH
KTLDKNSKKDTPRQGTSKKALLSGVLKCSRCGAPMRVTYGRKRKDGTSIYYYTCTMKAHS
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEEEEEECC
GKTRCDNPNVRGDYLEKNIITELKNLNSDVVIKGLEEHKKQLVATTENSIIKNISKEIEE
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
KKKEMDSLINKLSKIESDIASEFILSKVDSLGKEIKELEVNLLKRTDKRKENSDIELNIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHH
IVLQAIKEFNDFFNSVESFKDDELTIQRKRYLLERAVDEIRIDGETNGIDIDLWRAKKK
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCCC
>Mature Secondary Structure
MKAAIYSRKSMFTGKGDSVENQVQMCKDYGNRLNVDEYLIYEDEGYSGGNINRPKFQELL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHH
QDVKKKKFDVLICYRLDRISRNVADFSSTLELLQDNNISFVSIKEQFDTSTPMGKAMVYI
HHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCHHHHHHHH
ASVFAQLERETIAERVRDNMLELAKSGRWLGGQTPLGFESEKISYFDAEMKERTMYKLSP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEECC
ESKELELVKLIYNKYLETGSIHLTLKYLLSNNIKGKNGGEFASMSINDILRNPVYAKSNE
CCHHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCH
MVLNYLKDKGMNVCGTANGNGILIYNKRNSKYKRKDINEWVAAVSKHKGIIPANVWIEVQ
HHHHHHHHCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHEEEEH
KTLDKNSKKDTPRQGTSKKALLSGVLKCSRCGAPMRVTYGRKRKDGTSIYYYTCTMKAHS
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEEEEEECC
GKTRCDNPNVRGDYLEKNIITELKNLNSDVVIKGLEEHKKQLVATTENSIIKNISKEIEE
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
KKKEMDSLINKLSKIESDIASEFILSKVDSLGKEIKELEVNLLKRTDKRKENSDIELNIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHH
IVLQAIKEFNDFFNSVESFKDDELTIQRKRYLLERAVDEIRIDGETNGIDIDLWRAKKK
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377 [H]