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The map label for this gene is mtbP [H]

Identifier: 148380278

GI number: 148380278

Start: 2478334

End: 2479356

Strand: Reverse

Name: mtbP [H]

Synonym: CBO2328

Alternate gene names: 148380278

Gene position: 2479356-2478334 (Counterclockwise)

Preceding gene: 148380279

Following gene: 148380277

Centisome position: 63.79

GC content: 28.84

Gene sequence:

>1023_bases
ATGAAAACAATAAAACTATTAAGTCTATTTAGTGGAATAGGAGCCTTTGAAAAGGCTCTATCTAATATAAATCAAGATCA
TGAAATAATAAACTATTGCGAGATAGATAAGTATGCTAGTTATGCTTACAGTGTACTTCATAATGCAAATGAAAGCTTAA
ATTTAGGTGATGTGTCTAAGATTAATCCAGATACATTAAAAGATTTTGATTTATTAACTCATGGAAGTCCTTGCCAAAGT
TTTAGCCTAGCAGGTAAGGGTGAAGGAGGAGACGAAGGAAGTGGAACAAAATCATCATTAATGTGGTATACAGTTGATAT
TATTAAAAAGAAATTACCTAAATATATAATTTGGGAAAATGTAAAGGCAGTAACATGTAAAAGGCATAAACATAATTTTT
CTAAATATATAGATACATTGAATAAATTAGGCTATAACTCTTACTGGAATATATTAAATTCTAAAGATTATGGATCACCA
CAATCTAGAGAAAGAATGTTTTGTATATCAATCAGAAAAGATATAGATGCCTGCTTATTTAAATTCCCTAAACCTATGGA
TAGTATTGTTCCAATAAATACTATATTAGATGAATATGTAGATAAAAAATATTATTGTATTAATAAATACACAAGAGAAT
TTATTAATAAAGTAGATAGAAAAGTAGTAGATAATAAGGAACCTAATAAATATGGCTTATTGAGAATAGGAAAAATCGAA
AATCCAACAGCACTTAATATGAATAAGAGGGTATTTTCGTCACAAGGGGCATGTCCTACTATTTTAACTGGCTCTCATAG
TGTTCCTAAGATCATGGAATACAAATTAAGGCGACTTACTCCATGTGAGAGCTGGAAGGCTACAGGATTTACAGAAGAAG
ATTACAATTTAGTTAAAACTAAACTAACTAATAAATTTTATAAAGGGGTTGATAGAACAGATACACAAATGTACAAAATG
GCTGGTAATAGCATTGTTGTACCTGTTCTAGAAACTATATTGAAAGAACTATTTAAAGCATAA

Upstream 100 bases:

>100_bases
TACGATACAAATAAATAATTTATAAAGGCAAAGTAATAGGCCATGCAGGTCTTTTTATTTTGCCTATTTTTACATCAAAA
GAAGAAAGAAGGGTATACAA

Downstream 100 bases:

>100_bases
TCCCTATTTATATGGAGGTGTAATGTGGAATTAAAAGTTTGCGAAGAAAAGCATAAAAGGATAGAAGAAAAAATAAATGT
GCATGATATTAGGCTTAACG

Product: C-5 cytosine-specific DNA methylase

Products: NA

Alternate protein names: M.Phi3TI; Cytosine-specific methyltransferase Phi3TI [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKTIKLLSLFSGIGAFEKALSNINQDHEIINYCEIDKYASYAYSVLHNANESLNLGDVSKINPDTLKDFDLLTHGSPCQS
FSLAGKGEGGDEGSGTKSSLMWYTVDIIKKKLPKYIIWENVKAVTCKRHKHNFSKYIDTLNKLGYNSYWNILNSKDYGSP
QSRERMFCISIRKDIDACLFKFPKPMDSIVPINTILDEYVDKKYYCINKYTREFINKVDRKVVDNKEPNKYGLLRIGKIE
NPTALNMNKRVFSSQGACPTILTGSHSVPKIMEYKLRRLTPCESWKATGFTEEDYNLVKTKLTNKFYKGVDRTDTQMYKM
AGNSIVVPVLETILKELFKA

Sequences:

>Translated_340_residues
MKTIKLLSLFSGIGAFEKALSNINQDHEIINYCEIDKYASYAYSVLHNANESLNLGDVSKINPDTLKDFDLLTHGSPCQS
FSLAGKGEGGDEGSGTKSSLMWYTVDIIKKKLPKYIIWENVKAVTCKRHKHNFSKYIDTLNKLGYNSYWNILNSKDYGSP
QSRERMFCISIRKDIDACLFKFPKPMDSIVPINTILDEYVDKKYYCINKYTREFINKVDRKVVDNKEPNKYGLLRIGKIE
NPTALNMNKRVFSSQGACPTILTGSHSVPKIMEYKLRRLTPCESWKATGFTEEDYNLVKTKLTNKFYKGVDRTDTQMYKM
AGNSIVVPVLETILKELFKA
>Mature_340_residues
MKTIKLLSLFSGIGAFEKALSNINQDHEIINYCEIDKYASYAYSVLHNANESLNLGDVSKINPDTLKDFDLLTHGSPCQS
FSLAGKGEGGDEGSGTKSSLMWYTVDIIKKKLPKYIIWENVKAVTCKRHKHNFSKYIDTLNKLGYNSYWNILNSKDYGSP
QSRERMFCISIRKDIDACLFKFPKPMDSIVPINTILDEYVDKKYYCINKYTREFINKVDRKVVDNKEPNKYGLLRIGKIE
NPTALNMNKRVFSSQGACPTILTGSHSVPKIMEYKLRRLTPCESWKATGFTEEDYNLVKTKLTNKFYKGVDRTDTQMYKM
AGNSIVVPVLETILKELFKA

Specific function: This enzyme methylates the first cytosine within the sequences GGCC and GCNGC [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=383, Percent_Identity=24.2819843342037, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 38903; Mature: 38903

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIKLLSLFSGIGAFEKALSNINQDHEIINYCEIDKYASYAYSVLHNANESLNLGDVSK
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
INPDTLKDFDLLTHGSPCQSFSLAGKGEGGDEGSGTKSSLMWYTVDIIKKKLPKYIIWEN
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
VKAVTCKRHKHNFSKYIDTLNKLGYNSYWNILNSKDYGSPQSRERMFCISIRKDIDACLF
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHH
KFPKPMDSIVPINTILDEYVDKKYYCINKYTREFINKVDRKVVDNKEPNKYGLLRIGKIE
HCCCCCCHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NPTALNMNKRVFSSQGACPTILTGSHSVPKIMEYKLRRLTPCESWKATGFTEEDYNLVKT
CCCCCCCCHHHHCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
KLTNKFYKGVDRTDTQMYKMAGNSIVVPVLETILKELFKA
HHHHHHHCCCCCCHHHHHHHCCCCEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTIKLLSLFSGIGAFEKALSNINQDHEIINYCEIDKYASYAYSVLHNANESLNLGDVSK
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
INPDTLKDFDLLTHGSPCQSFSLAGKGEGGDEGSGTKSSLMWYTVDIIKKKLPKYIIWEN
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
VKAVTCKRHKHNFSKYIDTLNKLGYNSYWNILNSKDYGSPQSRERMFCISIRKDIDACLF
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHH
KFPKPMDSIVPINTILDEYVDKKYYCINKYTREFINKVDRKVVDNKEPNKYGLLRIGKIE
HCCCCCCHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NPTALNMNKRVFSSQGACPTILTGSHSVPKIMEYKLRRLTPCESWKATGFTEEDYNLVKT
CCCCCCCCHHHHCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
KLTNKFYKGVDRTDTQMYKMAGNSIVVPVLETILKELFKA
HHHHHHHCCCCCCHHHHHHHCCCCEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]