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The map label for this gene is 148380275

Identifier: 148380275

GI number: 148380275

Start: 2477046

End: 2477807

Strand: Reverse

Name: 148380275

Synonym: CBO2325

Alternate gene names: NA

Gene position: 2477807-2477046 (Counterclockwise)

Preceding gene: 148380276

Following gene: 148380274

Centisome position: 63.75

GC content: 31.36

Gene sequence:

>762_bases
ATGAAAATAGGAATAGATTGTGGTCATACATTAAGTGGAGCTGATTATGGAGCAGTAGGAATAAAAGCAGAATCTAATCT
AACCAGGGAAGTAGGTATTAGAGTAATAAGTAAATTGCAGGCTTTAGGACATAAAGTTATTAAGTGCTATAAAGATACTT
GCAATAGTTTAAATGACAGTTTAAGTTATAGAACTAATACGGCTAATAATAACAATGTAGATTTATATGTATCTATTCAT
TTTAATTGTTATAACGGTAGTGCTTATGGTACTGAAGTATTTACATACGGAGGTAAGGAGCTACAACAAGCTAGAGCAGT
ATTAAATAATATTTGTGCTTTAGGATATACAAACAGAGGGTTAAAAGATGGTTCTAGTCTTTATGTATTGAAACATACAA
AATCTAAAGCAATGCTAATAGAATGTTGTTTCTGTGATAATGCAGGAGATATGAACAGATATAATGCTGAAAATATGGCT
AATGCTATAGTTAAAGGATTAGTAGGACAAACTACAAGTAGTACATCAAACAAGCCAACAGGTAACAGCGATAATGGATG
GATTAATTTAGATGGTAAAACAGGTGCTATAAATACACCAAGCGGTATAAATGTTAGAGCAGGTAAGTCTACAAGTTCTA
AAATATTAGGAACATTAGCTAATGGTGCAAAGGTTAATTTATATAGAAAAGAGGGAGATTGGATGCACATATATTATCCT
CCATGCGGTGGATATGTTTATGCTAAATATATAAGATATTAA

Upstream 100 bases:

>100_bases
ATTTTATTTTGGACAAAGTTCAGCTAGGCAGGCAGTAAAAGAAAGTAAAGAGCAGGAGTAAACCTGTTCTTTTATTATTA
AATTTTTAGGAGGTAACTTT

Downstream 100 bases:

>100_bases
TTTTAAAGGTGCTTCTGTAATGGGAGCACCTTATTTTTTATTTTTTGAAGGAATAGATAAATATATGTAGAAATATACTT
AATATTGGGAATATGAAGAG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MKIGIDCGHTLSGADYGAVGIKAESNLTREVGIRVISKLQALGHKVIKCYKDTCNSLNDSLSYRTNTANNNNVDLYVSIH
FNCYNGSAYGTEVFTYGGKELQQARAVLNNICALGYTNRGLKDGSSLYVLKHTKSKAMLIECCFCDNAGDMNRYNAENMA
NAIVKGLVGQTTSSTSNKPTGNSDNGWINLDGKTGAINTPSGINVRAGKSTSSKILGTLANGAKVNLYRKEGDWMHIYYP
PCGGYVYAKYIRY

Sequences:

>Translated_253_residues
MKIGIDCGHTLSGADYGAVGIKAESNLTREVGIRVISKLQALGHKVIKCYKDTCNSLNDSLSYRTNTANNNNVDLYVSIH
FNCYNGSAYGTEVFTYGGKELQQARAVLNNICALGYTNRGLKDGSSLYVLKHTKSKAMLIECCFCDNAGDMNRYNAENMA
NAIVKGLVGQTTSSTSNKPTGNSDNGWINLDGKTGAINTPSGINVRAGKSTSSKILGTLANGAKVNLYRKEGDWMHIYYP
PCGGYVYAKYIRY
>Mature_253_residues
MKIGIDCGHTLSGADYGAVGIKAESNLTREVGIRVISKLQALGHKVIKCYKDTCNSLNDSLSYRTNTANNNNVDLYVSIH
FNCYNGSAYGTEVFTYGGKELQQARAVLNNICALGYTNRGLKDGSSLYVLKHTKSKAMLIECCFCDNAGDMNRYNAENMA
NAIVKGLVGQTTSSTSNKPTGNSDNGWINLDGKTGAINTPSGINVRAGKSTSSKILGTLANGAKVNLYRKEGDWMHIYYP
PCGGYVYAKYIRY

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To C.perfringens pIP404 ORF10 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 27424; Mature: 27424

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIDCGHTLSGADYGAVGIKAESNLTREVGIRVISKLQALGHKVIKCYKDTCNSLNDS
CEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LSYRTNTANNNNVDLYVSIHFNCYNGSAYGTEVFTYGGKELQQARAVLNNICALGYTNRG
CEEEECCCCCCCEEEEEEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCC
LKDGSSLYVLKHTKSKAMLIECCFCDNAGDMNRYNAENMANAIVKGLVGQTTSSTSNKPT
CCCCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
GNSDNGWINLDGKTGAINTPSGINVRAGKSTSSKILGTLANGAKVNLYRKEGDWMHIYYP
CCCCCCEEEECCCCCCCCCCCCCEEEECCCCCHHHHEEECCCEEEEEEEECCCEEEEEEC
PCGGYVYAKYIRY
CCCCEEEEEEECC
>Mature Secondary Structure
MKIGIDCGHTLSGADYGAVGIKAESNLTREVGIRVISKLQALGHKVIKCYKDTCNSLNDS
CEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LSYRTNTANNNNVDLYVSIHFNCYNGSAYGTEVFTYGGKELQQARAVLNNICALGYTNRG
CEEEECCCCCCCEEEEEEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCC
LKDGSSLYVLKHTKSKAMLIECCFCDNAGDMNRYNAENMANAIVKGLVGQTTSSTSNKPT
CCCCCEEEEEEECCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
GNSDNGWINLDGKTGAINTPSGINVRAGKSTSSKILGTLANGAKVNLYRKEGDWMHIYYP
CCCCCCEEEECCCCCCCCCCCCCEEEECCCCCHHHHEEECCCEEEEEEEECCCEEEEEEC
PCGGYVYAKYIRY
CCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8052128; 11792842 [H]