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The map label for this gene is pflC [H]

Identifier: 148380072

GI number: 148380072

Start: 2254357

End: 2255319

Strand: Reverse

Name: pflC [H]

Synonym: CBO2117

Alternate gene names: 148380072

Gene position: 2255319-2254357 (Counterclockwise)

Preceding gene: 148380073

Following gene: 148380071

Centisome position: 58.02

GC content: 29.8

Gene sequence:

>963_bases
ATGAGTAATATGAGTAATGGAAATTTAGGTATAATCGAGAGAAAAGCAAGGATTTTTAATATACAAAAATATAATATGTA
TGATGGAGATGGAATAAGAACTTTAGTATTTTTTCAAGGATGTCCACTTAGATGTAAATGGTGTGCAAATCCTGAAGGAC
TAGAAAAAAAATATAGAGTTATGCTTAAAAGTAATCTATGTGTAAATTGTGGAGCTTGTGTTTCTGCATGTCCTGTAGGA
ATCCATACTATTTCTAATAAAACTCTAAAGCATGAAGTTAATCGTGACATAGATTGTATAGGATGTGGAAAGTGTAAAGA
AGCTTGTCTTAAATCTGCTATATCAATAGTTGGAGAAGAAAAAACTATCTCTGAACTTTTAAAAATAGTAGAAGAAGATA
GAACTTTTTATGAAATGTCCGGCGGTGGAGTTACATTAGGTGGTGGCGAAGTTTTAATGCAGCCTGAAGCTGCTACTAGT
TTACTAATGGCATGTAAGCAAGAAGGTATAAACACTGCAATTGAAACTTGTGGCTATACAAATCTTGAAACAATCCTTAA
GGTTGCAGAATTTGTAGATCTATTCTTATTTGATATTAAAAATATTAATTCTGATAGACATCATGAATTAACTGGAGTAA
GAAATGAGAGGATTTTAGAAAATCTTCAAGAATTACTCAAGAAAAAATACAATGTAAAGATTCGTATGCCTTTACTTAAA
GGTATAAATGATTCTCAAGATGAGATAGAAAAAACTATGGAATTTTTATTACCTTATAAAGATTATAAAAACTTTAAGGG
AATTGATTTACTTCCTTATCATAAGATGGGGGTAAATAAATATAACCAATTAGGAATGGAATATCCTATAAAAGATGATC
CAAGCCTTAAAAGTGAAGATTTAGATAGAATAGAAGGATGGATTAAAAAATATGATTTACCTGTAAAGGTAATCCGCCAT
TAA

Upstream 100 bases:

>100_bases
TAAAATAATTTATATAAAGTCTGCAATCTACAACTTCTTTTGTAGATTGCAGATACCAATTAAGATATATATTAAAAATC
AAAGTAAATGAAACTGGAGA

Downstream 100 bases:

>100_bases
ATAATTTTCAAATAGATAATAAGATGGAAGGTAAGGTTTATGGGAGATTTTTCTGATAGAAATATACAGCGTGTTATACA
AGAGTCAGTGCCAGGAAAGC

Product: glycyl-radical activating family protein

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 2; PFL-activating enzyme 2 [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSNMSNGNLGIIERKARIFNIQKYNMYDGDGIRTLVFFQGCPLRCKWCANPEGLEKKYRVMLKSNLCVNCGACVSACPVG
IHTISNKTLKHEVNRDIDCIGCGKCKEACLKSAISIVGEEKTISELLKIVEEDRTFYEMSGGGVTLGGGEVLMQPEAATS
LLMACKQEGINTAIETCGYTNLETILKVAEFVDLFLFDIKNINSDRHHELTGVRNERILENLQELLKKKYNVKIRMPLLK
GINDSQDEIEKTMEFLLPYKDYKNFKGIDLLPYHKMGVNKYNQLGMEYPIKDDPSLKSEDLDRIEGWIKKYDLPVKVIRH

Sequences:

>Translated_320_residues
MSNMSNGNLGIIERKARIFNIQKYNMYDGDGIRTLVFFQGCPLRCKWCANPEGLEKKYRVMLKSNLCVNCGACVSACPVG
IHTISNKTLKHEVNRDIDCIGCGKCKEACLKSAISIVGEEKTISELLKIVEEDRTFYEMSGGGVTLGGGEVLMQPEAATS
LLMACKQEGINTAIETCGYTNLETILKVAEFVDLFLFDIKNINSDRHHELTGVRNERILENLQELLKKKYNVKIRMPLLK
GINDSQDEIEKTMEFLLPYKDYKNFKGIDLLPYHKMGVNKYNQLGMEYPIKDDPSLKSEDLDRIEGWIKKYDLPVKVIRH
>Mature_319_residues
SNMSNGNLGIIERKARIFNIQKYNMYDGDGIRTLVFFQGCPLRCKWCANPEGLEKKYRVMLKSNLCVNCGACVSACPVGI
HTISNKTLKHEVNRDIDCIGCGKCKEACLKSAISIVGEEKTISELLKIVEEDRTFYEMSGGGVTLGGGEVLMQPEAATSL
LMACKQEGINTAIETCGYTNLETILKVAEFVDLFLFDIKNINSDRHHELTGVRNERILENLQELLKKKYNVKIRMPLLKG
INDSQDEIEKTMEFLLPYKDYKNFKGIDLLPYHKMGVNKYNQLGMEYPIKDDPSLKSEDLDRIEGWIKKYDLPVKVIRH

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI1790389, Length=302, Percent_Identity=34.7682119205298, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI226510931, Length=286, Percent_Identity=36.013986013986, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1787130, Length=196, Percent_Identity=36.2244897959184, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790839, Length=271, Percent_Identity=25.830258302583, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006638
- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 36336; Mature: 36205

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS01087 RADICAL_ACTIVATING ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNMSNGNLGIIERKARIFNIQKYNMYDGDGIRTLVFFQGCPLRCKWCANPEGLEKKYRV
CCCCCCCCEEEEECHHEEEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCHHHHHHHHH
MLKSNLCVNCGACVSACPVGIHTISNKTLKHEVNRDIDCIGCGKCKEACLKSAISIVGEE
HHHCCCEEECCHHHHHCCCCEEHHCCCHHHHHHCCCCCEEECCHHHHHHHHHHHHHHCCH
KTISELLKIVEEDRTFYEMSGGGVTLGGGEVLMQPEAATSLLMACKQEGINTAIETCGYT
HHHHHHHHHHHCCCCEEEECCCEEEECCCCEEECCHHHHHHHHHHHHHCHHHHHHHCCCC
NLETILKVAEFVDLFLFDIKNINSDRHHELTGVRNERILENLQELLKKKYNVKIRMPLLK
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCEEEEEHHHC
GINDSQDEIEKTMEFLLPYKDYKNFKGIDLLPYHKMGVNKYNQLGMEYPIKDDPSLKSED
CCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHCCCHHHHCCCCCCCCCCCCCCHHH
LDRIEGWIKKYDLPVKVIRH
HHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
SNMSNGNLGIIERKARIFNIQKYNMYDGDGIRTLVFFQGCPLRCKWCANPEGLEKKYRV
CCCCCCCEEEEECHHEEEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCHHHHHHHHH
MLKSNLCVNCGACVSACPVGIHTISNKTLKHEVNRDIDCIGCGKCKEACLKSAISIVGEE
HHHCCCEEECCHHHHHCCCCEEHHCCCHHHHHHCCCCCEEECCHHHHHHHHHHHHHHCCH
KTISELLKIVEEDRTFYEMSGGGVTLGGGEVLMQPEAATSLLMACKQEGINTAIETCGYT
HHHHHHHHHHHCCCCEEEECCCEEEECCCCEEECCHHHHHHHHHHHHHCHHHHHHHCCCC
NLETILKVAEFVDLFLFDIKNINSDRHHELTGVRNERILENLQELLKKKYNVKIRMPLLK
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCEEEEEHHHC
GINDSQDEIEKTMEFLLPYKDYKNFKGIDLLPYHKMGVNKYNQLGMEYPIKDDPSLKSED
CCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHCCCHHHHCCCCCCCCCCCCCCHHH
LDRIEGWIKKYDLPVKVIRH
HHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]