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The map label for this gene is 148379710

Identifier: 148379710

GI number: 148379710

Start: 1862461

End: 1863222

Strand: Direct

Name: 148379710

Synonym: CBO1751

Alternate gene names: NA

Gene position: 1862461-1863222 (Clockwise)

Preceding gene: 148379709

Following gene: 148379711

Centisome position: 47.92

GC content: 30.97

Gene sequence:

>762_bases
ATGAAAATAGGAATTGATTGTGGACATACAATGTCAGGTGCTGATTATGGAGCAGTAGGAATAAAAGCAGAATCTAATTT
GACTAGAGAGGTAGGGACAAAAGTAATAAGTAAATTACAAGTTTTAGGTCATACGGTTATTAAGTGTTATAAAGATACTT
GTAGTAGTTTAAATGATAGTTTAAGTTATAGAACTAATACAGCCAATAATAACAATGTAGATTTATATGTATCTATTCAT
TTTAATTGCTATAATGGTAGTGCTTATGGTGCAGAGGTTTTTACTTATGGCGGAAAATCTTTTACAGAAGCGTCAAGAGT
ATTAAATAATATATGTGCTTTAGGTTATACAAATAGAGGGATAAAAGATGGTAGTAATTTATATGTACTAAAACACACAA
AGGCTAAAGCCATGCTTATAGAATGTTGTTTCTGTGATAATGCAGGAGATATGAACAGATATAATGCTGAAAATATGGCT
AATGCTATAGTTAAAGGATTAGTAGGACAGACTACAAGTAGTACACCAAGCAAACCAACAGATAACAACAATAATAGCTG
GATTAATTTAGATGGTAAAACAGGTACTATATGTACCCCAAGCGGTGTAAATATTAGAGAAAAGAAATCTACATCTAGCA
GAATATTAGGTGCTTTACCTAATGGTGCAAAAGTACAATTATACCGTAAAGAAGGAGATTGGATACATATTTATTATCCT
CCGCATGGTGGTTATGTTTATGAGAAATATATAAGATATTAA

Upstream 100 bases:

>100_bases
TTTTACTTTGGACAAAGTTCAGCAAGACAAGCGGTGAAAGAAAGTAAAGAGCAGGAATAAACCTGTTCTTTTTTTATATT
AAATTTTAGGAGGTAATATT

Downstream 100 bases:

>100_bases
ATTTTTAAAGGTACTTCTGTAATGGAAGTACCTTCTTTTTTTATTTTGCTGAATAATAATGTAAAATGTCTGAATTTTTG
GTAAAAATTATAAAGGAAAA

Product: bacteriophage endolysin (N-acetylmuramoyl-L-alanine amidase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MKIGIDCGHTMSGADYGAVGIKAESNLTREVGTKVISKLQVLGHTVIKCYKDTCSSLNDSLSYRTNTANNNNVDLYVSIH
FNCYNGSAYGAEVFTYGGKSFTEASRVLNNICALGYTNRGIKDGSNLYVLKHTKAKAMLIECCFCDNAGDMNRYNAENMA
NAIVKGLVGQTTSSTPSKPTDNNNNSWINLDGKTGTICTPSGVNIREKKSTSSRILGALPNGAKVQLYRKEGDWIHIYYP
PHGGYVYEKYIRY

Sequences:

>Translated_253_residues
MKIGIDCGHTMSGADYGAVGIKAESNLTREVGTKVISKLQVLGHTVIKCYKDTCSSLNDSLSYRTNTANNNNVDLYVSIH
FNCYNGSAYGAEVFTYGGKSFTEASRVLNNICALGYTNRGIKDGSNLYVLKHTKAKAMLIECCFCDNAGDMNRYNAENMA
NAIVKGLVGQTTSSTPSKPTDNNNNSWINLDGKTGTICTPSGVNIREKKSTSSRILGALPNGAKVQLYRKEGDWIHIYYP
PHGGYVYEKYIRY
>Mature_253_residues
MKIGIDCGHTMSGADYGAVGIKAESNLTREVGTKVISKLQVLGHTVIKCYKDTCSSLNDSLSYRTNTANNNNVDLYVSIH
FNCYNGSAYGAEVFTYGGKSFTEASRVLNNICALGYTNRGIKDGSNLYVLKHTKAKAMLIECCFCDNAGDMNRYNAENMA
NAIVKGLVGQTTSSTPSKPTDNNNNSWINLDGKTGTICTPSGVNIREKKSTSSRILGALPNGAKVQLYRKEGDWIHIYYP
PHGGYVYEKYIRY

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To C.perfringens pIP404 ORF10 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 27697; Mature: 27697

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIDCGHTMSGADYGAVGIKAESNLTREVGTKVISKLQVLGHTVIKCYKDTCSSLNDS
CEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LSYRTNTANNNNVDLYVSIHFNCYNGSAYGAEVFTYGGKSFTEASRVLNNICALGYTNRG
CEEEECCCCCCCEEEEEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCC
IKDGSNLYVLKHTKAKAMLIECCFCDNAGDMNRYNAENMANAIVKGLVGQTTSSTPSKPT
CCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
DNNNNSWINLDGKTGTICTPSGVNIREKKSTSSRILGALPNGAKVQLYRKEGDWIHIYYP
CCCCCCEEEECCCCCCEECCCCCCEECCCCCCCCEEEECCCCCEEEEEEECCCEEEEEEC
PHGGYVYEKYIRY
CCCCEEHHHHHCC
>Mature Secondary Structure
MKIGIDCGHTMSGADYGAVGIKAESNLTREVGTKVISKLQVLGHTVIKCYKDTCSSLNDS
CEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LSYRTNTANNNNVDLYVSIHFNCYNGSAYGAEVFTYGGKSFTEASRVLNNICALGYTNRG
CEEEECCCCCCCEEEEEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCC
IKDGSNLYVLKHTKAKAMLIECCFCDNAGDMNRYNAENMANAIVKGLVGQTTSSTPSKPT
CCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
DNNNNSWINLDGKTGTICTPSGVNIREKKSTSSRILGALPNGAKVQLYRKEGDWIHIYYP
CCCCCCEEEECCCCCCEECCCCCCEECCCCCCCCEEEECCCCCEEEEEEECCCEEEEEEC
PHGGYVYEKYIRY
CCCCEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8052128; 11792842 [H]