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The map label for this gene is sigV [H]

Identifier: 148378828

GI number: 148378828

Start: 945263

End: 945775

Strand: Direct

Name: sigV [H]

Synonym: CBO0836

Alternate gene names: 148378828

Gene position: 945263-945775 (Clockwise)

Preceding gene: 148378827

Following gene: 148378829

Centisome position: 24.32

GC content: 26.71

Gene sequence:

>513_bases
ATGAATACAGAGGAGTTGGCTATCAAAGCAAAAAGCGGAGATGAAAACGCTTTTTGTAAGCTTATAAAAATTAATAAAGA
GGTTCTATATAAAACAGCGTTTTATTATACAAAGAACGAACATGATTCATTAGAAATATTAGATGATACCGTTTATAAAG
CCTATAAATCAATTAAAAAATTAAAACAGGAAAAATATTTTAATACTTGGCTAATGAGAATACTCATAAATAGCTGTATC
ACCCACATAAATAAAAGAAAAAGGTTGATATTTTTTAGAAAAAATATTGATATTACTGAAAAACATGAATCAGTTGATAA
CAGAGAAGAATTATTGGATTTATATAAAGCTATTGATACATTGGAGGACAAGTTTAAAATGATAATAATACTTAGGTACT
TTAATGATTTAACTGTTCCTCAGATAGCTGATATTATGGACTACCCTATTGGTACAGTAAAGACTTATATTCATAAGGCT
TTAAAACAACTAAGAATTGAATTGGAGGTATAG

Upstream 100 bases:

>100_bases
AAATTTGAAAATAAGTAAAACGAAAGTATAGTTTGAAATGTCTAATAGATGACAAGTTATTACCGGTAATAATATTATAT
CTTGCTTGGAGGATTACTAT

Downstream 100 bases:

>100_bases
GAATATGGATTATGACAAGAAATCTTTTAGAGAAAAACTAGAATTAATAGAAGTGCCAGAGGAAGTTGATTTAGTTATAG
ATAAGGCTATTAAGAGAGCT

Product: RNA polymerase sigma-70 factor, ECF family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MNTEELAIKAKSGDENAFCKLIKINKEVLYKTAFYYTKNEHDSLEILDDTVYKAYKSIKKLKQEKYFNTWLMRILINSCI
THINKRKRLIFFRKNIDITEKHESVDNREELLDLYKAIDTLEDKFKMIIILRYFNDLTVPQIADIMDYPIGTVKTYIHKA
LKQLRIELEV

Sequences:

>Translated_170_residues
MNTEELAIKAKSGDENAFCKLIKINKEVLYKTAFYYTKNEHDSLEILDDTVYKAYKSIKKLKQEKYFNTWLMRILINSCI
THINKRKRLIFFRKNIDITEKHESVDNREELLDLYKAIDTLEDKFKMIIILRYFNDLTVPQIADIMDYPIGTVKTYIHKA
LKQLRIELEV
>Mature_170_residues
MNTEELAIKAKSGDENAFCKLIKINKEVLYKTAFYYTKNEHDSLEILDDTVYKAYKSIKKLKQEKYFNTWLMRILINSCI
THINKRKRLIFFRKNIDITEKHESVDNREELLDLYKAIDTLEDKFKMIIILRYFNDLTVPQIADIMDYPIGTVKTYIHKA
LKQLRIELEV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Positively regulates the expression of proteins involved in stress responses against bacitracin, paraquat and tellurite

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=167, Percent_Identity=24.5508982035928, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 20334; Mature: 20334

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTEELAIKAKSGDENAFCKLIKINKEVLYKTAFYYTKNEHDSLEILDDTVYKAYKSIKK
CCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHH
LKQEKYFNTWLMRILINSCITHINKRKRLIFFRKNIDITEKHESVDNREELLDLYKAIDT
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHH
LEDKFKMIIILRYFNDLTVPQIADIMDYPIGTVKTYIHKALKQLRIELEV
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTEELAIKAKSGDENAFCKLIKINKEVLYKTAFYYTKNEHDSLEILDDTVYKAYKSIKK
CCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHH
LKQEKYFNTWLMRILINSCITHINKRKRLIFFRKNIDITEKHESVDNREELLDLYKAIDT
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHH
LEDKFKMIIILRYFNDLTVPQIADIMDYPIGTVKTYIHKALKQLRIELEV
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]