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The map label for this gene is leuC2 [H]

Identifier: 148378421

GI number: 148378421

Start: 498495

End: 500420

Strand: Direct

Name: leuC2 [H]

Synonym: CBO0418

Alternate gene names: 148378421

Gene position: 498495-500420 (Clockwise)

Preceding gene: 148378420

Following gene: 148378422

Centisome position: 12.82

GC content: 31.83

Gene sequence:

>1926_bases
TTGAGTTTAACATTAACAGAAAAGATAATAAAATCACATTTAGTAAAAGGAAATATGACTAAAGGAGAAGAAATTGCTAT
TAAAATAGATAATACTTTAACTCAGGATTCTACGGGAACTATGGCATATCTTCAGTTTGAAGCTTTAGGTATAGATAAAG
TAAAGACTAAAAGATCAGTGGCTTATATAGACCACAATATGTTGCAAAGTGGACCAGAGAATGCGGATGACCATTTATAT
ATACAAACAGTAGCTAAAAAACATGGAATTTATTTTTCAAAACCAGGTAATGGAATATGTCATCAAGTTAATTTGGAAAG
ATTTGGTGTGCCTGGAGAAACTTTATTAGGATCCGATAGCCATACTCCAACAGGAGGAGGTATAGGAATGTTAGCTATAG
GTGCTGGTGGATTGGATGTAGCAGTAGCTATGGCAGGTGGAGAATATTATATAAATATGCCTAAAGTAGTTAAGGTAAAT
TTAATAGGAAAATTAAAACCTTTTGTATCTGCAAAAGATATTATCTTAGAAGTTTTAAGAAGATTATCTGTAAAGGGTGG
AGTAGGAAAAATATTTGAATATACAGGAGAAGGAGTAAAAGCTCTTACAGTTCCAGAGAGAGCAACTATAACTAATATGG
GAGCAGAACTTGGAGCTACTACATCAATTTTTCCATCAGATGAAATTACTAAAGATTTCTTAAAAGCTCAAGAAAGAGAA
GAAGATTATAAGTATCTAGTGGCAGATGAGGATGCTTTCTATGATGAGGAATTAGAAATAAATTTATCAGATCTAAAACC
TTTAGCAGCCTGTCCACACAGCCCTGATAATATAGTACCTGTATCAGAACTTAAAAATATAAAAGTTAACCAAGTAGCTA
TAGGTAGTTGTACAAATTCTTCTTATAGAGATATGTTAAGAACAGCTAGTATATTAAAGGGGAAAACTGTATCAGAAAAT
GTATCTTTAGTTATAGCACCAGGCTCTAAACAAGTTCTAAACATGATGGCTAATAATGGAGCTTTAACAGATTTAGTTAT
GGCAGGAGCAAGAATACTAGAATGTGCCTGTGGACCATGTATAGGAATGGGACAATCTCCTTGTACAGATGCTGTATCTT
TAAGAACTTTTAATAGAAATTTTGAGGGTAGATCTGGAACAAAATCTGCTGATGTGTATTTAGTAAGTCCTGAAGTGGCA
GCAGTTTCAGCATTAACAGGATATATAACAGATCCCACTGAGTTAACTGATTTTGAAGATATAGAAATGCCTAATAAATT
TTTGATAAATGATAATTTAATAATAGAACCAGCTACAGAAGGAGAAGTTGTAGAGGTTAAAAGAGGACCAAATATAAAAC
CTTTCCCTAAAGCAGAAGCTTTAAAAGAATTAGTAACTGGAAAGGCATTAACTAAAGTAGGGGACAATATAACAACAGAT
CATATAATGCCATCTAATGCTAAACTTTTACCTTATAGATCTAATGTACCATATTTATCAAACTTTTGCTTAACTCCATG
TGATGAAAAATTCCCTGAAAAAGCTAAGGAAAATGGAGGAGGCATAATAGTCGCAGGAACAAATTACGGACAAGGTTCTA
GTAGAGAACATGCAGCCTTAGCCCCTGTTTATTTAGGTATAAAAGCAGTTTTAGCAAAATCCTTTGCTAGAATTCATAAA
GCAAATCTAATAAATAATGGTATATTACCTTTAGTTTTTGAAAGAGAAGAAGATTATGATTCTATAGATGAAATGGATGA
GTTAGTAATAGAAAATGTACTAAATCAAGTGGAAATAGGTAAAATAGATATAATAAATAAAACTAAGAGCATAAAATATA
AAACTTTATTAGAAGCAACAGATAGACAAAAGAAGATGATTTTAAAGGGCGGATTATTAAATTTAATAAAAAGTTCTCAA
CAATAG

Upstream 100 bases:

>100_bases
AACCCTATAAAATAAAAACTAAAGAGAGTATAAATAAAAAATTAGTAAGTAAAAAACAAAAGATAAATATTAAAGTATAA
ATTGATTGGGGGAATAACAG

Downstream 100 bases:

>100_bases
CTACAAAAATTTAGTTAAGTTTTCGGAGGGATATGATGAAACACAATATAACTTTAATACCAGGAGATGGTATAGGACCA
GAAGTAACTGAAGCTGCAAG

Product: aconitate hydratase

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 641; Mature: 640

Protein sequence:

>641_residues
MSLTLTEKIIKSHLVKGNMTKGEEIAIKIDNTLTQDSTGTMAYLQFEALGIDKVKTKRSVAYIDHNMLQSGPENADDHLY
IQTVAKKHGIYFSKPGNGICHQVNLERFGVPGETLLGSDSHTPTGGGIGMLAIGAGGLDVAVAMAGGEYYINMPKVVKVN
LIGKLKPFVSAKDIILEVLRRLSVKGGVGKIFEYTGEGVKALTVPERATITNMGAELGATTSIFPSDEITKDFLKAQERE
EDYKYLVADEDAFYDEELEINLSDLKPLAACPHSPDNIVPVSELKNIKVNQVAIGSCTNSSYRDMLRTASILKGKTVSEN
VSLVIAPGSKQVLNMMANNGALTDLVMAGARILECACGPCIGMGQSPCTDAVSLRTFNRNFEGRSGTKSADVYLVSPEVA
AVSALTGYITDPTELTDFEDIEMPNKFLINDNLIIEPATEGEVVEVKRGPNIKPFPKAEALKELVTGKALTKVGDNITTD
HIMPSNAKLLPYRSNVPYLSNFCLTPCDEKFPEKAKENGGGIIVAGTNYGQGSSREHAALAPVYLGIKAVLAKSFARIHK
ANLINNGILPLVFEREEDYDSIDEMDELVIENVLNQVEIGKIDIINKTKSIKYKTLLEATDRQKKMILKGGLLNLIKSSQ
Q

Sequences:

>Translated_641_residues
MSLTLTEKIIKSHLVKGNMTKGEEIAIKIDNTLTQDSTGTMAYLQFEALGIDKVKTKRSVAYIDHNMLQSGPENADDHLY
IQTVAKKHGIYFSKPGNGICHQVNLERFGVPGETLLGSDSHTPTGGGIGMLAIGAGGLDVAVAMAGGEYYINMPKVVKVN
LIGKLKPFVSAKDIILEVLRRLSVKGGVGKIFEYTGEGVKALTVPERATITNMGAELGATTSIFPSDEITKDFLKAQERE
EDYKYLVADEDAFYDEELEINLSDLKPLAACPHSPDNIVPVSELKNIKVNQVAIGSCTNSSYRDMLRTASILKGKTVSEN
VSLVIAPGSKQVLNMMANNGALTDLVMAGARILECACGPCIGMGQSPCTDAVSLRTFNRNFEGRSGTKSADVYLVSPEVA
AVSALTGYITDPTELTDFEDIEMPNKFLINDNLIIEPATEGEVVEVKRGPNIKPFPKAEALKELVTGKALTKVGDNITTD
HIMPSNAKLLPYRSNVPYLSNFCLTPCDEKFPEKAKENGGGIIVAGTNYGQGSSREHAALAPVYLGIKAVLAKSFARIHK
ANLINNGILPLVFEREEDYDSIDEMDELVIENVLNQVEIGKIDIINKTKSIKYKTLLEATDRQKKMILKGGLLNLIKSSQ
Q
>Mature_640_residues
SLTLTEKIIKSHLVKGNMTKGEEIAIKIDNTLTQDSTGTMAYLQFEALGIDKVKTKRSVAYIDHNMLQSGPENADDHLYI
QTVAKKHGIYFSKPGNGICHQVNLERFGVPGETLLGSDSHTPTGGGIGMLAIGAGGLDVAVAMAGGEYYINMPKVVKVNL
IGKLKPFVSAKDIILEVLRRLSVKGGVGKIFEYTGEGVKALTVPERATITNMGAELGATTSIFPSDEITKDFLKAQEREE
DYKYLVADEDAFYDEELEINLSDLKPLAACPHSPDNIVPVSELKNIKVNQVAIGSCTNSSYRDMLRTASILKGKTVSENV
SLVIAPGSKQVLNMMANNGALTDLVMAGARILECACGPCIGMGQSPCTDAVSLRTFNRNFEGRSGTKSADVYLVSPEVAA
VSALTGYITDPTELTDFEDIEMPNKFLINDNLIIEPATEGEVVEVKRGPNIKPFPKAEALKELVTGKALTKVGDNITTDH
IMPSNAKLLPYRSNVPYLSNFCLTPCDEKFPEKAKENGGGIIVAGTNYGQGSSREHAALAPVYLGIKAVLAKSFARIHKA
NLINNGILPLVFEREEDYDSIDEMDELVIENVLNQVEIGKIDIINKTKSIKYKTLLEATDRQKKMILKGGLLNLIKSSQQ

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=721, Percent_Identity=34.6740638002774, Blast_Score=314, Evalue=2e-85,
Organism=Homo sapiens, GI8659555, Length=732, Percent_Identity=26.9125683060109, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI41352693, Length=660, Percent_Identity=27.4242424242424, Blast_Score=179, Evalue=7e-45,
Organism=Escherichia coli, GI87081781, Length=723, Percent_Identity=27.5242047026279, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=28.3261802575107, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1787531, Length=631, Percent_Identity=27.2583201267829, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI2367097, Length=462, Percent_Identity=25.3246753246753, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25149337, Length=715, Percent_Identity=35.3846153846154, Blast_Score=315, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI32564738, Length=679, Percent_Identity=35.7879234167894, Blast_Score=306, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI25149342, Length=565, Percent_Identity=35.2212389380531, Blast_Score=251, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17568399, Length=762, Percent_Identity=26.509186351706, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6322261, Length=690, Percent_Identity=34.3478260869565, Blast_Score=344, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6323335, Length=727, Percent_Identity=33.1499312242091, Blast_Score=325, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6320440, Length=608, Percent_Identity=32.7302631578947, Blast_Score=243, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6321429, Length=667, Percent_Identity=26.9865067466267, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI281365315, Length=724, Percent_Identity=35.3591160220994, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI17864292, Length=724, Percent_Identity=35.3591160220994, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI28571643, Length=721, Percent_Identity=34.2579750346741, Blast_Score=311, Evalue=7e-85,
Organism=Drosophila melanogaster, GI161076999, Length=688, Percent_Identity=35.9011627906977, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24645686, Length=759, Percent_Identity=25.9552042160738, Blast_Score=170, Evalue=3e-42,
Organism=Drosophila melanogaster, GI17137564, Length=724, Percent_Identity=25.9668508287293, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 69493; Mature: 69362

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTLTEKIIKSHLVKGNMTKGEEIAIKIDNTLTQDSTGTMAYLQFEALGIDKVKTKRSV
CCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCHHHHCCCE
AYIDHNMLQSGPENADDHLYIQTVAKKHGIYFSKPGNGICHQVNLERFGVPGETLLGSDS
EEEEHHHHHCCCCCCCCEEEEEEEHHHCCEEEECCCCCCEEEECHHHCCCCCCCEECCCC
HTPTGGGIGMLAIGAGGLDVAVAMAGGEYYINMPKVVKVNLIGKLKPFVSAKDIILEVLR
CCCCCCCEEEEEEECCCCEEEEEEECCEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHH
RLSVKGGVGKIFEYTGEGVKALTVPERATITNMGAELGATTSIFPSDEITKDFLKAQERE
HHCCCCCCCHHHHCCCCCEEEEECCCCHHHHHCCHHHCCCEECCCCHHHHHHHHHHHHCC
EDYKYLVADEDAFYDEELEINLSDLKPLAACPHSPDNIVPVSELKNIKVNQVAIGSCTNS
CCCEEEEECCCCCCCCEEEEEHHHCCHHHCCCCCCCCEEEHHHHCCCEEEEEEEECCCCH
SYRDMLRTASILKGKTVSENVSLVIAPGSKQVLNMMANNGALTDLVMAGARILECACGPC
HHHHHHHHHHHHCCCEECCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
IGMGQSPCTDAVSLRTFNRNFEGRSGTKSADVYLVSPEVAAVSALTGYITDPTELTDFED
CCCCCCCCCCHHEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCHH
IEMPNKFLINDNLIIEPATEGEVVEVKRGPNIKPFPKAEALKELVTGKALTKVGDNITTD
CCCCCEEEECCCEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCC
HIMPSNAKLLPYRSNVPYLSNFCLTPCDEKFPEKAKENGGGIIVAGTNYGQGSSREHAAL
CCCCCCCEEEEECCCCCHHHCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCH
APVYLGIKAVLAKSFARIHKANLINNGILPLVFEREEDYDSIDEMDELVIENVLNQVEIG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
KIDIINKTKSIKYKTLLEATDRQKKMILKGGLLNLIKSSQQ
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLTLTEKIIKSHLVKGNMTKGEEIAIKIDNTLTQDSTGTMAYLQFEALGIDKVKTKRSV
CCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCHHHHCCCE
AYIDHNMLQSGPENADDHLYIQTVAKKHGIYFSKPGNGICHQVNLERFGVPGETLLGSDS
EEEEHHHHHCCCCCCCCEEEEEEEHHHCCEEEECCCCCCEEEECHHHCCCCCCCEECCCC
HTPTGGGIGMLAIGAGGLDVAVAMAGGEYYINMPKVVKVNLIGKLKPFVSAKDIILEVLR
CCCCCCCEEEEEEECCCCEEEEEEECCEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHH
RLSVKGGVGKIFEYTGEGVKALTVPERATITNMGAELGATTSIFPSDEITKDFLKAQERE
HHCCCCCCCHHHHCCCCCEEEEECCCCHHHHHCCHHHCCCEECCCCHHHHHHHHHHHHCC
EDYKYLVADEDAFYDEELEINLSDLKPLAACPHSPDNIVPVSELKNIKVNQVAIGSCTNS
CCCEEEEECCCCCCCCEEEEEHHHCCHHHCCCCCCCCEEEHHHHCCCEEEEEEEECCCCH
SYRDMLRTASILKGKTVSENVSLVIAPGSKQVLNMMANNGALTDLVMAGARILECACGPC
HHHHHHHHHHHHCCCEECCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
IGMGQSPCTDAVSLRTFNRNFEGRSGTKSADVYLVSPEVAAVSALTGYITDPTELTDFED
CCCCCCCCCCHHEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCHH
IEMPNKFLINDNLIIEPATEGEVVEVKRGPNIKPFPKAEALKELVTGKALTKVGDNITTD
CCCCCEEEECCCEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCC
HIMPSNAKLLPYRSNVPYLSNFCLTPCDEKFPEKAKENGGGIIVAGTNYGQGSSREHAAL
CCCCCCCEEEEECCCCCHHHCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCH
APVYLGIKAVLAKSFARIHKANLINNGILPLVFEREEDYDSIDEMDELVIENVLNQVEIG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
KIDIINKTKSIKYKTLLEATDRQKKMILKGGLLNLIKSSQQ
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]