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The map label for this gene is leuA2 [H]

Identifier: 148378420

GI number: 148378420

Start: 496993

End: 498351

Strand: Direct

Name: leuA2 [H]

Synonym: CBO0417

Alternate gene names: 148378420

Gene position: 496993-498351 (Clockwise)

Preceding gene: 148378419

Following gene: 148378421

Centisome position: 12.79

GC content: 31.27

Gene sequence:

>1359_bases
ATGGTTAAAATAGTTAAGTCTTCATATAATCATGTACTTCAAAATATATCAAGTCCTAATTTGTATAAGGATCTATTTCC
ATATGATGAAATACCAAAGGTGACATTTAATGATATACAGGTTCCAATGGATTTACCAAAAGATATATGGATTACTGATA
CAACATTTAGAGATGGACAACAATCAATGCCACCATATACTACAGAACAAATAGTAAAAATATATGACTATCTTCATAAA
TTAGACAATGAATCTGGGGTAATAAGACAAACAGAATTTTTCTTGTATTCTGAGAAAGACAGAAAGGCAGCAGAAATATG
TTTAGATAGAGGCTATAAGTTTCCAGAAGTTACTTCATGGATAAGAGCTAGCAAAGAAGATTTTAAATTGGTTAAGGAAA
TGGGAATAAAAGAAACTGGCATGCTTATGTCTTGTTCAGATTACCATATATTTAAGAAACTTAATACAACAAGAGAAAAG
GCTATGAGTATGTATTTAGAAATCGCGGAAGAAGCTTTAGCTAATGGAATTGTTCCAAGATGCCATTTAGAAGATATAAC
AAGAGCGGATTTTTATGGGTTTGTAGTTCCTTTTGTAAATAAATTGATGGAACTCTCCAATAAAGCTAATATACCTATTA
AAATAAGAGCCTGTGACACTTTAGGATTAGGGGTTCCTTATAGTGGAGCATCACTACCAAGAAGTGTTCAAGGAATCATT
CATGGACTTAGATATTGTTGTAATGTTCCTTCAGAAAGAATAGAGTGGCATGGACACAATGATTTTTATGGTGTAGTAGC
TAATGCTTCCACATCATGGCTTTATGGCTGCTCATCTGTAAATACTTCTCTTTTAGGTATAGGCGAAAGAACGGGAAATT
GTCCTCTAGAAGCTATGATTATAGAATATGGACAATTAAAAGGTAATACCGCTAATATGAACTTACATGTTATAAATGAA
ATAGCAGAATATTTTGAGAGAGAAATGAATTATAATATACCATCAAGAACACCTTTTGTAGGAAGTGAGTTTAATGTTAC
AAGAGCTGGTATTCATGCAGATGGTATATTAAAAAATGAGGAAATATATAATATTTTCAATACAGAAAAGATTTTAGACA
GACCAGTTGTAGTTGCAGTAAATCAGTATTCAGGCTTAGCTGGTATTGCAGCTTGGATAAACACTTACTTTAAATTAAAA
GGTGAAAATAAAATAGATAAAAAAGATGAAAGAATTGTGGCTATAAAAGAATGGGTAGATAATCAATATGAAAATGGCAG
AACAACAATAATAGGAAATAATGAATTGGAAGTTATAGTAAAGCAACTTATACCGGATTTATTTAGAAGAGCTAGCTAG

Upstream 100 bases:

>100_bases
ATATTATCTAAAATGAAGGAAAAGAATTAAAAAATGCATAATATAGTTTTTTGAGTAAAATATGGGGGAAAACCAATAAA
TAAATTTAGGGGGAATTATG

Downstream 100 bases:

>100_bases
TAATTACCATATAGTTTCATAAAATAAAAAAATATTTAAATAAAACCCTATAAAATAAAAACTAAAGAGAGTATAAATAA
AAAATTAGTAAGTAAAAAAC

Product: HMGL-related enzyme

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MVKIVKSSYNHVLQNISSPNLYKDLFPYDEIPKVTFNDIQVPMDLPKDIWITDTTFRDGQQSMPPYTTEQIVKIYDYLHK
LDNESGVIRQTEFFLYSEKDRKAAEICLDRGYKFPEVTSWIRASKEDFKLVKEMGIKETGMLMSCSDYHIFKKLNTTREK
AMSMYLEIAEEALANGIVPRCHLEDITRADFYGFVVPFVNKLMELSNKANIPIKIRACDTLGLGVPYSGASLPRSVQGII
HGLRYCCNVPSERIEWHGHNDFYGVVANASTSWLYGCSSVNTSLLGIGERTGNCPLEAMIIEYGQLKGNTANMNLHVINE
IAEYFEREMNYNIPSRTPFVGSEFNVTRAGIHADGILKNEEIYNIFNTEKILDRPVVVAVNQYSGLAGIAAWINTYFKLK
GENKIDKKDERIVAIKEWVDNQYENGRTTIIGNNELEVIVKQLIPDLFRRAS

Sequences:

>Translated_452_residues
MVKIVKSSYNHVLQNISSPNLYKDLFPYDEIPKVTFNDIQVPMDLPKDIWITDTTFRDGQQSMPPYTTEQIVKIYDYLHK
LDNESGVIRQTEFFLYSEKDRKAAEICLDRGYKFPEVTSWIRASKEDFKLVKEMGIKETGMLMSCSDYHIFKKLNTTREK
AMSMYLEIAEEALANGIVPRCHLEDITRADFYGFVVPFVNKLMELSNKANIPIKIRACDTLGLGVPYSGASLPRSVQGII
HGLRYCCNVPSERIEWHGHNDFYGVVANASTSWLYGCSSVNTSLLGIGERTGNCPLEAMIIEYGQLKGNTANMNLHVINE
IAEYFEREMNYNIPSRTPFVGSEFNVTRAGIHADGILKNEEIYNIFNTEKILDRPVVVAVNQYSGLAGIAAWINTYFKLK
GENKIDKKDERIVAIKEWVDNQYENGRTTIIGNNELEVIVKQLIPDLFRRAS
>Mature_452_residues
MVKIVKSSYNHVLQNISSPNLYKDLFPYDEIPKVTFNDIQVPMDLPKDIWITDTTFRDGQQSMPPYTTEQIVKIYDYLHK
LDNESGVIRQTEFFLYSEKDRKAAEICLDRGYKFPEVTSWIRASKEDFKLVKEMGIKETGMLMSCSDYHIFKKLNTTREK
AMSMYLEIAEEALANGIVPRCHLEDITRADFYGFVVPFVNKLMELSNKANIPIKIRACDTLGLGVPYSGASLPRSVQGII
HGLRYCCNVPSERIEWHGHNDFYGVVANASTSWLYGCSSVNTSLLGIGERTGNCPLEAMIIEYGQLKGNTANMNLHVINE
IAEYFEREMNYNIPSRTPFVGSEFNVTRAGIHADGILKNEEIYNIFNTEKILDRPVVVAVNQYSGLAGIAAWINTYFKLK
GENKIDKKDERIVAIKEWVDNQYENGRTTIIGNNELEVIVKQLIPDLFRRAS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=363, Percent_Identity=26.7217630853994, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320019, Length=351, Percent_Identity=25.3561253561254, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320071, Length=355, Percent_Identity=24.7887323943662, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 51560; Mature: 51560

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIVKSSYNHVLQNISSPNLYKDLFPYDEIPKVTFNDIQVPMDLPKDIWITDTTFRDGQ
CEEEEHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCCCEEEECCCCCCCC
QSMPPYTTEQIVKIYDYLHKLDNESGVIRQTEFFLYSEKDRKAAEICLDRGYKFPEVTSW
CCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHHCCCCCHHHHHH
IRASKEDFKLVKEMGIKETGMLMSCSDYHIFKKLNTTREKAMSMYLEIAEEALANGIVPR
HHCCHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
CHLEDITRADFYGFVVPFVNKLMELSNKANIPIKIRACDTLGLGVPYSGASLPRSVQGII
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECEECCCCCCCCCCCCHHHHHHH
HGLRYCCNVPSERIEWHGHNDFYGVVANASTSWLYGCSSVNTSLLGIGERTGNCPLEAMI
HHHHHHHCCCHHHEEECCCCCEEEEEECCCCCEEEECCCCCCEEEECCCCCCCCCHHHHE
IEYGQLKGNTANMNLHVINEIAEYFEREMNYNIPSRTPFVGSEFNVTRAGIHADGILKNE
EHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCC
EIYNIFNTEKILDRPVVVAVNQYSGLAGIAAWINTYFKLKGENKIDKKDERIVAIKEWVD
HHHHHHCHHHHHCCCEEEEEECCCCHHHHHHHHHHHEEECCCCCCCCCCCCEEEHHHHHC
NQYENGRTTIIGNNELEVIVKQLIPDLFRRAS
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKIVKSSYNHVLQNISSPNLYKDLFPYDEIPKVTFNDIQVPMDLPKDIWITDTTFRDGQ
CEEEEHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCCCEEEECCCCCCCC
QSMPPYTTEQIVKIYDYLHKLDNESGVIRQTEFFLYSEKDRKAAEICLDRGYKFPEVTSW
CCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHHHHHHCCCCCHHHHHH
IRASKEDFKLVKEMGIKETGMLMSCSDYHIFKKLNTTREKAMSMYLEIAEEALANGIVPR
HHCCHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
CHLEDITRADFYGFVVPFVNKLMELSNKANIPIKIRACDTLGLGVPYSGASLPRSVQGII
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECEECCCCCCCCCCCCHHHHHHH
HGLRYCCNVPSERIEWHGHNDFYGVVANASTSWLYGCSSVNTSLLGIGERTGNCPLEAMI
HHHHHHHCCCHHHEEECCCCCEEEEEECCCCCEEEECCCCCCEEEECCCCCCCCCHHHHE
IEYGQLKGNTANMNLHVINEIAEYFEREMNYNIPSRTPFVGSEFNVTRAGIHADGILKNE
EHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCC
EIYNIFNTEKILDRPVVVAVNQYSGLAGIAAWINTYFKLKGENKIDKKDERIVAIKEWVD
HHHHHHCHHHHHCCCEEEEEECCCCHHHHHHHHHHHEEECCCCCCCCCCCCEEEHHHHHC
NQYENGRTTIIGNNELEVIVKQLIPDLFRRAS
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]