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The map label for this gene is eno [H]

Identifier: 148378233

GI number: 148378233

Start: 272163

End: 273458

Strand: Direct

Name: eno [H]

Synonym: CBO0230

Alternate gene names: 148378233

Gene position: 272163-273458 (Clockwise)

Preceding gene: 148378232

Following gene: 148378234

Centisome position: 7.0

GC content: 33.33

Gene sequence:

>1296_bases
ATGAAAAATTATATTGAAATAGTAGATGTATACGCAAGACAAATCTTAGATTCAAGATGCAACCCTACAGTAGAAGTAGA
AGTAGAATTAGAAGACGGAACAGTAGGAGTAGCAGCAGTTCCATCAGGAGCTTCTACAGGAGCCTTTGAAGCAGTTGAAT
TAAGAGATGGAGATAAATCTAAATACTTAGGTAAAGGCGTTTTAAAAGCTGTAGATAATGTAAACACTATAATAGCAGAT
GAACTTGTTGGAATGAATGTTTTAGATCAAGTAGCTATTGATAAAACTATGATAGAATTAGATGGAACAGACAATAAAGC
AAAATTAGGTGCAAATGCAATGCTAGGAGTATCTTTAGCTTGTGCAAAAGCTGCAGCTAACTCTTTAGGAATAAGTTTAT
ACCAATACATAGGAGGAGTAAATGGAAAAGTTTTACCAGTACCTATGATGAACATAATAAATGGTGGAAAACATGCGGAC
AACAATGTTGATCTTCAAGAATTCATGATAATGCCAGCAGGAGCTCCTTCTTTCAGTGAAGCTTTAAGAATGTGTTCAGA
AGTATATCATGCATTAAAATCAACATTAAAAGCACAAGGATATGATACAGGGGTAGGCGACGAAGGTGGATTTGCTCCAA
ACTTAAAATCAAATGAAGAAGCTATAGTAGTTATAATAGAAGCTATAAAAAAAGCTGGATATACTCCAGGAGAAGATATA
TTCATAGCTTTAGATCCAGCTTCATCAGAAATATTTGAAGATGGAAAATACAATCTAGCAGGAGAAGGAAGAGTATTAAC
TCCAGAAGAAATGGCAAACTACTATGTAGAATTAGCAGAAAAATACCCAATAATCTCTATAGAAGACGGTATGGCAGAAG
AAGATTGGGATGGTTGGAAAATCCTTACTGAAAAAATAGGAAACAAAGTTCAATTAGTAGGAGACGATTTATTCGTTACA
AATACTGAAAGATTATCTAAAGGAATAAAATTAGGAGTTGCAAACTCAATTCTTATAAAACTTAACCAAATAGGAACATT
AACAGAAACATTAAATGCTATAGAAATGGCAGAAAGAGCTGGATATACAGCAGTAGTATCTCATAGATCAGGAGAAACTG
AAGATACAACAATAGCTGACTTAGTTGTTGCAGTAAATGCAGGACAAATAAAAACAGGTGCTCCAGCAAGATCAGAAAGA
GTTGCTAAATACAATCAATTATTAAGAATAGAAGAAGAACTTAATGATATGGGAGAATACAGAGGATTAAAAGCATTCTA
CAATATCAATAAATAA

Upstream 100 bases:

>100_bases
CTTCCTAAACCCTCTGAAATGACAGGGAATAGTCTTATTAAATAATAGGGAAACTATATTTAAGATAAAATAATTTTTTT
AAAAATAAGGAGGACCCAAC

Downstream 100 bases:

>100_bases
TAATTTTTAAAATAAGGGGCTGCTCATAGTATGGGTAGTCCTTTAATCTATAAAAATGTAAATATAAAAGTATATATGTA
ATTAAAATATAAATTAATCC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKSKYLGKGVLKAVDNVNTIIAD
ELVGMNVLDQVAIDKTMIELDGTDNKAKLGANAMLGVSLACAKAAANSLGISLYQYIGGVNGKVLPVPMMNIINGGKHAD
NNVDLQEFMIMPAGAPSFSEALRMCSEVYHALKSTLKAQGYDTGVGDEGGFAPNLKSNEEAIVVIIEAIKKAGYTPGEDI
FIALDPASSEIFEDGKYNLAGEGRVLTPEEMANYYVELAEKYPIISIEDGMAEEDWDGWKILTEKIGNKVQLVGDDLFVT
NTERLSKGIKLGVANSILIKLNQIGTLTETLNAIEMAERAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSER
VAKYNQLLRIEEELNDMGEYRGLKAFYNINK

Sequences:

>Translated_431_residues
MKNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKSKYLGKGVLKAVDNVNTIIAD
ELVGMNVLDQVAIDKTMIELDGTDNKAKLGANAMLGVSLACAKAAANSLGISLYQYIGGVNGKVLPVPMMNIINGGKHAD
NNVDLQEFMIMPAGAPSFSEALRMCSEVYHALKSTLKAQGYDTGVGDEGGFAPNLKSNEEAIVVIIEAIKKAGYTPGEDI
FIALDPASSEIFEDGKYNLAGEGRVLTPEEMANYYVELAEKYPIISIEDGMAEEDWDGWKILTEKIGNKVQLVGDDLFVT
NTERLSKGIKLGVANSILIKLNQIGTLTETLNAIEMAERAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSER
VAKYNQLLRIEEELNDMGEYRGLKAFYNINK
>Mature_431_residues
MKNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKSKYLGKGVLKAVDNVNTIIAD
ELVGMNVLDQVAIDKTMIELDGTDNKAKLGANAMLGVSLACAKAAANSLGISLYQYIGGVNGKVLPVPMMNIINGGKHAD
NNVDLQEFMIMPAGAPSFSEALRMCSEVYHALKSTLKAQGYDTGVGDEGGFAPNLKSNEEAIVVIIEAIKKAGYTPGEDI
FIALDPASSEIFEDGKYNLAGEGRVLTPEEMANYYVELAEKYPIISIEDGMAEEDWDGWKILTEKIGNKVQLVGDDLFVT
NTERLSKGIKLGVANSILIKLNQIGTLTETLNAIEMAERAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSER
VAKYNQLLRIEEELNDMGEYRGLKAFYNINK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=52.3255813953488, Blast_Score=434, Evalue=1e-121,
Organism=Homo sapiens, GI4503571, Length=436, Percent_Identity=51.605504587156, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=51.7401392111369, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=51.7401392111369, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=425, Percent_Identity=46.8235294117647, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI169201331, Length=354, Percent_Identity=26.5536723163842, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI169201757, Length=354, Percent_Identity=26.5536723163842, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI239744207, Length=354, Percent_Identity=26.5536723163842, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=63.2075471698113, Blast_Score=520, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=53.5962877030162, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=53.5962877030162, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=48.6772486772487, Blast_Score=182, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=53.5796766743649, Blast_Score=415, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6324974, Length=442, Percent_Identity=51.131221719457, Blast_Score=408, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324969, Length=442, Percent_Identity=51.131221719457, Blast_Score=408, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6323985, Length=442, Percent_Identity=50.9049773755656, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=52.8868360277136, Blast_Score=389, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580918, Length=425, Percent_Identity=53.1764705882353, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580916, Length=425, Percent_Identity=53.1764705882353, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580920, Length=425, Percent_Identity=53.1764705882353, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580914, Length=425, Percent_Identity=53.1764705882353, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI281360527, Length=418, Percent_Identity=53.5885167464115, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI17137654, Length=418, Percent_Identity=53.5885167464115, Blast_Score=408, Evalue=1e-114,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46388; Mature: 46388

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKS
CCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCCCEEEEEECCCCCH
KYLGKGVLKAVDNVNTIIADELVGMNVLDQVAIDKTMIELDGTDNKAKLGANAMLGVSLA
HHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHCCEEEEECCCCCCHHCCCCHHHHHHHH
CAKAAANSLGISLYQYIGGVNGKVLPVPMMNIINGGKHADNNVDLQEFMIMPAGAPSFSE
HHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHCCCCCCCCCCCHHHEEEECCCCCCHHH
ALRMCSEVYHALKSTLKAQGYDTGVGDEGGFAPNLKSNEEAIVVIIEAIKKAGYTPGEDI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCCCCE
FIALDPASSEIFEDGKYNLAGEGRVLTPEEMANYYVELAEKYPIISIEDGMAEEDWDGWK
EEEECCCCHHHHCCCCEEECCCCCEECHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHH
ILTEKIGNKVQLVGDDLFVTNTERLSKGIKLGVANSILIKLNQIGTLTETLNAIEMAERA
HHHHHCCCEEEEEECCEEEECHHHHHCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHC
GYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSERVAKYNQLLRIEEELNDMGEY
CCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RGLKAFYNINK
CCEEEEEECCC
>Mature Secondary Structure
MKNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKS
CCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCCCEEEEEECCCCCH
KYLGKGVLKAVDNVNTIIADELVGMNVLDQVAIDKTMIELDGTDNKAKLGANAMLGVSLA
HHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHCCEEEEECCCCCCHHCCCCHHHHHHHH
CAKAAANSLGISLYQYIGGVNGKVLPVPMMNIINGGKHADNNVDLQEFMIMPAGAPSFSE
HHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHCCCCCCCCCCCHHHEEEECCCCCCHHH
ALRMCSEVYHALKSTLKAQGYDTGVGDEGGFAPNLKSNEEAIVVIIEAIKKAGYTPGEDI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCCCCE
FIALDPASSEIFEDGKYNLAGEGRVLTPEEMANYYVELAEKYPIISIEDGMAEEDWDGWK
EEEECCCCHHHHCCCCEEECCCCCEECHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHH
ILTEKIGNKVQLVGDDLFVTNTERLSKGIKLGVANSILIKLNQIGTLTETLNAIEMAERA
HHHHHCCCEEEEEECCEEEECHHHHHCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHC
GYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSERVAKYNQLLRIEEELNDMGEY
CCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RGLKAFYNINK
CCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA