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The map label for this gene is maeB [C]

Identifier: 148378213

GI number: 148378213

Start: 248077

End: 249249

Strand: Direct

Name: maeB [C]

Synonym: CBO0210

Alternate gene names: 148378213

Gene position: 248077-249249 (Clockwise)

Preceding gene: 148378212

Following gene: 148378217

Centisome position: 6.38

GC content: 31.2

Gene sequence:

>1173_bases
ATGGATATCAAAGAAAAATCACTAATGGTACATAAAAAATTTAAAGGCAAATTATCTATAGAGGGAAAAATACAAGTAAA
AAATAAAGAAGATTTATCTATAGCATATACTCCAGGAGTTGCAGAACCCTGCGTAAAAATAAATGAAGATAAATCTTTAG
TTTATGAATATACAATGAAGGGAAATACAGTAGCTGTAGTAACTAATGGTACAGCAGTTTTAGGTTTAGGGGATATAGGG
CCTTATGCAGGATTGCCTGTTATGGAAGGAAAAGCTCTTTTATTTAAGGAATTTGCAGATATAGATTCATTTCCAATTTG
TATAGATAGTAAAGACTCTGAAGAAATTATAAAAACAGTTAAACTTATAGCTCCAGGATTTGGAGGAATAAATTTAGAGG
ACATAAAAGCACCAGAATGCTTTTATATAGAAAAAAAATTAAAAGAGGAATTAGATATTCCAGTGTTTCATGATGATCAG
CATGGAACAGCCATAGTTGTATTAGCAGGAATATATAATGCTTTAAGACTTGTAGGGAAAAAATTAGAAGAAGCAAGAAT
AGTTATAAATGGTGCAGGCTCTGCAGGAATATCTATATGTAAATTGTTATTACAAGCAGGTGTTAAAAACATAATAATGT
GTGATAAAGAAGGGTCTTTAGTAAAAGGTAATAGTAATTTAAATGAAGCACAAAAATTAATAGTCGAAGTAACAAACAAA
GAAAATGAGAAAGGAACATTAAAAGACATAATAAAAGGAAAAGATGTATTTATAGGTGTATCAGCCCCAAATATACTTAC
AGAAGAAATGGTAAACGCTATGAACAAAGATTCCATAGTATTTGCTATGGCAAACCCAACTCCAGAGATAATGCCGGATA
AAGCAAAAAAGGCAGGAGCCAGAGTAGTAGCTACAGGAAGATCTGATTTCCCAAATCAAATAAATAATGTGTTAGTATTC
CCAGGAATTTTTAGAGGAGCATTAGATGTAAGATCTAAAGTTATAAATGAGGAAATGAAACTAGCAGCATCTAAAGCTAT
AGCTTCTTTGGTACAGGATAATGAATTAAATGAAGAGTACATAATACCAGGAGCCTTTGATAAAAGAGTAGCAGAAGTTG
TGGCAGAAGAAGTAAAAAAAGTAGCCTTAAAGATGGGATTATCAAAATTATAA

Upstream 100 bases:

>100_bases
GCTATTAAAGATATCCACATGTGGATAATTTTTTTTAGATTTTGTTCATAAATTAAATAAATCCTATATACTTATATACA
ATGAAAGAGGGGATAATAAT

Downstream 100 bases:

>100_bases
AATAAGGAAGTTGCGTAGCAACTTCCTTATTTTAAGTTTCTTCTTAAAAAGTCTAATGCTCTAGTTACAGTTATATTTCT
TATAAATTGTCTGTTACCAG

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MDIKEKSLMVHKKFKGKLSIEGKIQVKNKEDLSIAYTPGVAEPCVKINEDKSLVYEYTMKGNTVAVVTNGTAVLGLGDIG
PYAGLPVMEGKALLFKEFADIDSFPICIDSKDSEEIIKTVKLIAPGFGGINLEDIKAPECFYIEKKLKEELDIPVFHDDQ
HGTAIVVLAGIYNALRLVGKKLEEARIVINGAGSAGISICKLLLQAGVKNIIMCDKEGSLVKGNSNLNEAQKLIVEVTNK
ENEKGTLKDIIKGKDVFIGVSAPNILTEEMVNAMNKDSIVFAMANPTPEIMPDKAKKAGARVVATGRSDFPNQINNVLVF
PGIFRGALDVRSKVINEEMKLAASKAIASLVQDNELNEEYIIPGAFDKRVAEVVAEEVKKVALKMGLSKL

Sequences:

>Translated_390_residues
MDIKEKSLMVHKKFKGKLSIEGKIQVKNKEDLSIAYTPGVAEPCVKINEDKSLVYEYTMKGNTVAVVTNGTAVLGLGDIG
PYAGLPVMEGKALLFKEFADIDSFPICIDSKDSEEIIKTVKLIAPGFGGINLEDIKAPECFYIEKKLKEELDIPVFHDDQ
HGTAIVVLAGIYNALRLVGKKLEEARIVINGAGSAGISICKLLLQAGVKNIIMCDKEGSLVKGNSNLNEAQKLIVEVTNK
ENEKGTLKDIIKGKDVFIGVSAPNILTEEMVNAMNKDSIVFAMANPTPEIMPDKAKKAGARVVATGRSDFPNQINNVLVF
PGIFRGALDVRSKVINEEMKLAASKAIASLVQDNELNEEYIIPGAFDKRVAEVVAEEVKKVALKMGLSKL
>Mature_390_residues
MDIKEKSLMVHKKFKGKLSIEGKIQVKNKEDLSIAYTPGVAEPCVKINEDKSLVYEYTMKGNTVAVVTNGTAVLGLGDIG
PYAGLPVMEGKALLFKEFADIDSFPICIDSKDSEEIIKTVKLIAPGFGGINLEDIKAPECFYIEKKLKEELDIPVFHDDQ
HGTAIVVLAGIYNALRLVGKKLEEARIVINGAGSAGISICKLLLQAGVKNIIMCDKEGSLVKGNSNLNEAQKLIVEVTNK
ENEKGTLKDIIKGKDVFIGVSAPNILTEEMVNAMNKDSIVFAMANPTPEIMPDKAKKAGARVVATGRSDFPNQINNVLVF
PGIFRGALDVRSKVINEEMKLAASKAIASLVQDNELNEEYIIPGAFDKRVAEVVAEEVKKVALKMGLSKL

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=411, Percent_Identity=26.2773722627737, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI62420880, Length=411, Percent_Identity=26.2773722627737, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI239049447, Length=411, Percent_Identity=26.2773722627737, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI4505143, Length=410, Percent_Identity=25.8536585365854, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI4505145, Length=412, Percent_Identity=27.1844660194175, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI270265879, Length=371, Percent_Identity=27.4932614555256, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1788806, Length=405, Percent_Identity=48.1481481481481, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI87081919, Length=484, Percent_Identity=25.6198347107438, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17537199, Length=450, Percent_Identity=26.4444444444444, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6322823, Length=345, Percent_Identity=26.9565217391304, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281362674, Length=442, Percent_Identity=27.6018099547511, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281362672, Length=442, Percent_Identity=27.6018099547511, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356279, Length=442, Percent_Identity=27.6018099547511, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI78707242, Length=433, Percent_Identity=25.635103926097, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI78707238, Length=433, Percent_Identity=25.635103926097, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI78707240, Length=431, Percent_Identity=24.8259860788863, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI78707232, Length=378, Percent_Identity=25.6613756613757, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281363503, Length=378, Percent_Identity=25.6613756613757, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281363505, Length=378, Percent_Identity=25.6613756613757, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI78707236, Length=378, Percent_Identity=25.6613756613757, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 42289; Mature: 42289

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00331 MALIC_ENZYMES ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKEKSLMVHKKFKGKLSIEGKIQVKNKEDLSIAYTPGVAEPCVKINEDKSLVYEYTMK
CCCCHHHHHEEECCCCEEEEEEEEEECCCCCCEEEECCCCCCCCEEECCCCEEEEEEEEC
GNTVAVVTNGTAVLGLGDIGPYAGLPVMEGKALLFKEFADIDSFPICIDSKDSEEIIKTV
CCEEEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCCHHHHHHHH
KLIAPGFGGINLEDIKAPECFYIEKKLKEELDIPVFHDDQHGTAIVVLAGIYNALRLVGK
HHHCCCCCCCCHHHCCCCCEEHHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHHHH
KLEEARIVINGAGSAGISICKLLLQAGVKNIIMCDKEGSLVKGNSNLNEAQKLIVEVTNK
HHCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCHHHHHHHHHHCCCC
ENEKGTLKDIIKGKDVFIGVSAPNILTEEMVNAMNKDSIVFAMANPTPEIMPDKAKKAGA
CCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHCCC
RVVATGRSDFPNQINNVLVFPGIFRGALDVRSKVINEEMKLAASKAIASLVQDNELNEEY
EEEEECCCCCCHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IIPGAFDKRVAEVVAEEVKKVALKMGLSKL
ECCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIKEKSLMVHKKFKGKLSIEGKIQVKNKEDLSIAYTPGVAEPCVKINEDKSLVYEYTMK
CCCCHHHHHEEECCCCEEEEEEEEEECCCCCCEEEECCCCCCCCEEECCCCEEEEEEEEC
GNTVAVVTNGTAVLGLGDIGPYAGLPVMEGKALLFKEFADIDSFPICIDSKDSEEIIKTV
CCEEEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCCHHHHHHHH
KLIAPGFGGINLEDIKAPECFYIEKKLKEELDIPVFHDDQHGTAIVVLAGIYNALRLVGK
HHHCCCCCCCCHHHCCCCCEEHHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHHHH
KLEEARIVINGAGSAGISICKLLLQAGVKNIIMCDKEGSLVKGNSNLNEAQKLIVEVTNK
HHCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCHHHHHHHHHHCCCC
ENEKGTLKDIIKGKDVFIGVSAPNILTEEMVNAMNKDSIVFAMANPTPEIMPDKAKKAGA
CCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHCCC
RVVATGRSDFPNQINNVLVFPGIFRGALDVRSKVINEEMKLAASKAIASLVQDNELNEEY
EEEEECCCCCCHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IIPGAFDKRVAEVVAEEVKKVALKMGLSKL
ECCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]