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The map label for this gene is prfB [H]

Identifier: 148378178

GI number: 148378178

Start: 210125

End: 211102

Strand: Direct

Name: prfB [H]

Synonym: CBO0175

Alternate gene names: 148378178

Gene position: 210125-211102 (Clockwise)

Preceding gene: 148378177

Following gene: 148378180

Centisome position: 5.41

GC content: 30.37

Gene sequence:

>978_bases
ATGCAAAACCCCAATTTTTGGAATGATATAAAAGAGGCACAAAAAATAACTTCAGAAGAAAAGTATTTAAAGGATAAGTT
AACCAAATATAATCATTTAAGAAATACTATAGAAGATATTATATCTCTTTGTGAAATGTTATCAGAAGAAGATGATGATA
TGAAAAAAGAAATAATATCAGAATATAAAAATATAAAAGAAGAAACAGATAGATTTAAAATAGAAACCCTATTATCTGGA
GAGTATGATAGAAATAATGTAATAATGACCCTACATTCAGAAGTAGGAGGAACAGACGCGCAGGATTGGACAGATATGCT
ATTTAGAATGTATACAAGGTGGGCAGAAAAAGAAGGATTTGCTGTTAAGATTATGGATTTATTAGAAGCTGAAGATGCAG
GTATAAAAAGTGTAACTCTAAAGATTACTGGAGAGTTTGTATATGGATATCTTAAAAGTGAAAAGGGAATACACAGATTG
GTTAGAATATCTCCTTTTAATGCAAATGGTAAAAGACAGACTTCCTTTGCATCTATAGAAGTATTACCAGAACTAACAGA
TGATCAGGAAATAGAAATAAGAAGTGAAGACTTAAAAATAGATACCTATAGAGCTGGTGGAGCTGGAGGACAGCATGTTA
ATAAAACGGAATCTGCAGTAAGAATAACTCATATGCCAACAGGAATTATAGTACAATGCCAAAATGAAAGAAGTCAGCAT
ACTAACAAAGAAACAGCTATGAAGGTTTTAAAATCAAAATTAGTAGAACTAAAAGAAAGATCTCATAAAGAAAAAATAGA
AGATCTAACAGGAGAACTAAAGGATATGGGATGGGGAAGTCAAATAAGATCTTATGTATTTCATCCTTATAATCTAGTAA
AGGACCATAGAACAGGAGCAGAAACTAGCAATGTAGATTCAGTGATGAATGGTGATATAAATATATTCATAACACAGTAT
CTAAAAGGAAATTTTTAA

Upstream 100 bases:

>100_bases
ATAAATAACTTATATGAGCTTAAAAACACAATAAAAGAAATCGGGGAGTCTCTTTGACATAGAAAAATTAAAAAATAGTA
TATCAGAATTACAAAATAAA

Downstream 100 bases:

>100_bases
AATTAAAAGTTAAAAAATAAAGGGGATGTCTTAGAATAGATTTAATTTTAACATCATAAAAATAAAAATTAAATCTATTT
TATTTTTAAACATCCCTTTT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MQNPNFWNDIKEAQKITSEEKYLKDKLTKYNHLRNTIEDIISLCEMLSEEDDDMKKEIISEYKNIKEETDRFKIETLLSG
EYDRNNVIMTLHSEVGGTDAQDWTDMLFRMYTRWAEKEGFAVKIMDLLEAEDAGIKSVTLKITGEFVYGYLKSEKGIHRL
VRISPFNANGKRQTSFASIEVLPELTDDQEIEIRSEDLKIDTYRAGGAGGQHVNKTESAVRITHMPTGIIVQCQNERSQH
TNKETAMKVLKSKLVELKERSHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGAETSNVDSVMNGDINIFITQY
LKGNF

Sequences:

>Translated_325_residues
MQNPNFWNDIKEAQKITSEEKYLKDKLTKYNHLRNTIEDIISLCEMLSEEDDDMKKEIISEYKNIKEETDRFKIETLLSG
EYDRNNVIMTLHSEVGGTDAQDWTDMLFRMYTRWAEKEGFAVKIMDLLEAEDAGIKSVTLKITGEFVYGYLKSEKGIHRL
VRISPFNANGKRQTSFASIEVLPELTDDQEIEIRSEDLKIDTYRAGGAGGQHVNKTESAVRITHMPTGIIVQCQNERSQH
TNKETAMKVLKSKLVELKERSHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGAETSNVDSVMNGDINIFITQY
LKGNF
>Mature_325_residues
MQNPNFWNDIKEAQKITSEEKYLKDKLTKYNHLRNTIEDIISLCEMLSEEDDDMKKEIISEYKNIKEETDRFKIETLLSG
EYDRNNVIMTLHSEVGGTDAQDWTDMLFRMYTRWAEKEGFAVKIMDLLEAEDAGIKSVTLKITGEFVYGYLKSEKGIHRL
VRISPFNANGKRQTSFASIEVLPELTDDQEIEIRSEDLKIDTYRAGGAGGQHVNKTESAVRITHMPTGIIVQCQNERSQH
TNKETAMKVLKSKLVELKERSHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGAETSNVDSVMNGDINIFITQY
LKGNF

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=277, Percent_Identity=35.0180505415162, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI34577120, Length=288, Percent_Identity=33.3333333333333, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI166795305, Length=186, Percent_Identity=34.4086021505376, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI2367172, Length=329, Percent_Identity=46.8085106382979, Blast_Score=313, Evalue=7e-87,
Organism=Escherichia coli, GI1787462, Length=326, Percent_Identity=34.9693251533742, Blast_Score=185, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17542784, Length=314, Percent_Identity=28.6624203821656, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=293, Percent_Identity=30.3754266211604, Blast_Score=135, Evalue=7e-33,
Organism=Drosophila melanogaster, GI19921226, Length=287, Percent_Identity=35.8885017421603, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37462; Mature: 37462

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNPNFWNDIKEAQKITSEEKYLKDKLTKYNHLRNTIEDIISLCEMLSEEDDDMKKEIIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EYKNIKEETDRFKIETLLSGEYDRNNVIMTLHSEVGGTDAQDWTDMLFRMYTRWAEKEGF
HHHHHHHHHHHEEHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
AVKIMDLLEAEDAGIKSVTLKITGEFVYGYLKSEKGIHRLVRISPFNANGKRQTSFASIE
EEEHHHHHHHCCCCCEEEEEEEEHHHHHHHHHCCCCHHHHEEECCCCCCCCCCCCHHHEE
VLPELTDDQEIEIRSEDLKIDTYRAGGAGGQHVNKTESAVRITHMPTGIIVQCQNERSQH
ECCCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHC
TNKETAMKVLKSKLVELKERSHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCCCCC
ETSNVDSVMNGDINIFITQYLKGNF
CCCCCHHHHCCCEEEEEEECCCCCC
>Mature Secondary Structure
MQNPNFWNDIKEAQKITSEEKYLKDKLTKYNHLRNTIEDIISLCEMLSEEDDDMKKEIIS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EYKNIKEETDRFKIETLLSGEYDRNNVIMTLHSEVGGTDAQDWTDMLFRMYTRWAEKEGF
HHHHHHHHHHHEEHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
AVKIMDLLEAEDAGIKSVTLKITGEFVYGYLKSEKGIHRLVRISPFNANGKRQTSFASIE
EEEHHHHHHHCCCCCEEEEEEEEHHHHHHHHHCCCCHHHHEEECCCCCCCCCCCCHHHEE
VLPELTDDQEIEIRSEDLKIDTYRAGGAGGQHVNKTESAVRITHMPTGIIVQCQNERSQH
ECCCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHC
TNKETAMKVLKSKLVELKERSHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCCCCC
ETSNVDSVMNGDINIFITQYLKGNF
CCCCCHHHHCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA