Click here to switch to the map view.

The map label for this gene is gyrA [H]

Identifier: 148378018

GI number: 148378018

Start: 6342

End: 8852

Strand: Direct

Name: gyrA [H]

Synonym: CBO0007

Alternate gene names: 148378018

Gene position: 6342-8852 (Clockwise)

Preceding gene: 148378017

Following gene: 148378019

Centisome position: 0.16

GC content: 28.79

Gene sequence:

>2511_bases
ATGCTAAACGAAGGAAAGATTTTGCCTGTAGATGTAAGTAAAGAAATGAAGAAATGTTATATAGACTATGCCATGAGTGT
AATAGCTGGCCGTGCATTACCAGATGTAAGGGATGGGTTAAAGCCAGTACATAGAAGAATAATATATTCAATGCAGGGAT
TAGGATTAGCTCCAGAAAAAGGTTATAGAAAATGTGCAAGAATTGTAGGAGATGTACTGGGTAAGTATCATCCTCATGGA
GATACAGCAGTTTATGAAGCTTTAGTAAGAATGGCTCAGAATTTTTCAATAAGATATACATTAGTAGATGGTCATGGAAA
TTTTGGTTCTGTAGATGGTGATGGAGCAGCAGCAATGAGATATACAGAAGCTAAGATGAGTAAAATATCTATGGAACTTA
TAAAAGACATAAATAAAAATACAGTTGATTTTATTCCTAACTTTGATGGAGAAGAAGAAGAACCATCTGTATTACCATCA
AGGTTTCCTAATCTTTTAGTTAATGGTTCTTCAGGTATAGCAGTAGGTATGGCTACTAATATTCCACCTCATAATTTAAC
AGAGGTTATAGATGGAATAATAATGCTAATAGAAAATGAAGATGTAAATATACTTGATTTAATGACTAAAATAAAAGGAC
CAGATTTCCCTACTTCTGGATTAATCGTAGGTACAAGAGGAATAAGGGAAGCTTATGAAACGGGTAGGGGAAAAGTTATA
ATTAGAGCTAAAGCTGAGATTGAAGAGGAAAAAGGGAAAAATAAGATAATAGTTACAGAAATACCTTATCAAGTTAATAA
AGCTAGACTTATAGAAAATATGGCTAACCTTGTAAAGGATAAAAAAATAAATGGAATTTCAGATTTAAGAGATGAATCAG
ATAGAGATGGTATGAGAATTGTTATAGAATTAAAAAGGGATGCAAATCCTAATATAGTTTTAAACCAATTATATAAGCAT
ACAAAACTTCAAGATACCTTTGGAATAATTATGTTAGCTTTAGTTAATAACCAACCTCAAATTTTAAATTTAAAAGAAAT
ATTAGTTAATTATGTGGAATTCCAAAAGGAAGTAATAAGAAGAAGAACTAGATTTGACTTAGATAAAGCATTAGCAAGGG
CTCATATATTAGAAGGTTTAAGAATAGCCTTAGATCATATTGATGAAGTTATAAAGCTTATAAGAGCATCTAAAAATACA
GCTGAAGCTAAAGAGGGATTAATGAATAACTTTAATCTTTCAGAAAAACAAGCTCAAGCTATATTAGATATGAAGTTACA
AAGGCTTACAGGTTTAGAAAGAGAAAAAATAGAAGAAGAATATAAAGAACTTATGGAAAAGATAAGTTATTTTAGAGAAA
TATTGGATAAAGAAGAGTTAGTACTAAGTATAATAAAAGAAGAATTAATAGAGATAAAAAATAAATATGGTGATGAAAGA
AAAACAGAAATAGTAAAAGGTGAACATGATATAGATATAGAGGACTTAATTGAAGATAAAAAGGTAATAATAACTTTAAC
TCATGGTGGATACATAAAAAGATTAGATATGGATACATACTCTTCACAAAAAAGAGGAGGAAAGGGTATTCAAGCCACAT
CTACAAAACAGGATGATTTTATAGAAAATATGTTTGTAACATCTACTCATAGTACTATATTATTCTTTACAAATAGGGGT
AAGGTATATAAACTTAAAGCTTATGAAATACCAGAAGCAGGAAGAACAGCTAAAGGTACAAATATAGTAAATATTATACC
TATAGAAAATAATGAAAAAATACAAACAGTAATAGGATTAAAAGACATAGATGATATGAAACATTTTGTAATGTGTACTA
GAAATGGAATAATTAAAAAGACAGAAATAAAGAAATATTCTTCTATAAGAAAAGGTGGATTAAATGCCATAAATTTAAGA
GAAGATGATGAACTAATAGATGTAAAAATGACTAAAGGAAATAATGAAATAATAATAGTCACACAAAATGGGTACTGCAT
AAGATTTAATGAAGAAGATGTAAGGCCTATGGGAAGAGTTGCAACAGGAGTTAAAGGTATAACATTAAGAGAAGATGATA
AAGCTGTATCTATGGAGGTAGTGTTAGAAGATGAAGCACTACTATCAATAAGTGAAAATGGATTTGGAAAAAGAACAGAT
ATAGAAGAATATCCTATCCATAGAAGAGGTGGAAAAGGTGTTATAACTTATAAAATAACAGATAAAACAGGACCTATAGT
TGGAGCAAGGTTTATAAAAGAAGATGATGAACTTATGCTTGTAAATAGTGGTGATGTTGCAATAAGAATAAATGTTTCGG
AAATATCTAAAACTAGTAGAAATGCTATGGGTGTAAAACTAATGAGAACTAGTGAAGAAGAAAAAATAGTAGCTATAGCT
AAAATAAAGAGTGAAGACATAATAGAAGAAGAAATTTTAAATGAAGAAAATTTAAATGAAGAAAATTTAAATGAAGAAAA
TTTAAATGAAGAAAATTTAAATGAAGAATAA

Upstream 100 bases:

>100_bases
ATGGGAACAAAAGTAGAACCAAGAAGAGACTTTATAGAAAGTAATGCTGATAAAGTAGTTAACTTGGATATATAAGATAA
GATTGTAAAGAGGTGTAATC

Downstream 100 bases:

>100_bases
ATAATAAGAAAAATCAGCATTAATGCTGATTTTTCTTATTATAAATTTTAATTATAAAAAACAACTTTTCCAGACGTATC
AATATAAGAAATATAAATAA

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 836; Mature: 836

Protein sequence:

>836_residues
MLNEGKILPVDVSKEMKKCYIDYAMSVIAGRALPDVRDGLKPVHRRIIYSMQGLGLAPEKGYRKCARIVGDVLGKYHPHG
DTAVYEALVRMAQNFSIRYTLVDGHGNFGSVDGDGAAAMRYTEAKMSKISMELIKDINKNTVDFIPNFDGEEEEPSVLPS
RFPNLLVNGSSGIAVGMATNIPPHNLTEVIDGIIMLIENEDVNILDLMTKIKGPDFPTSGLIVGTRGIREAYETGRGKVI
IRAKAEIEEEKGKNKIIVTEIPYQVNKARLIENMANLVKDKKINGISDLRDESDRDGMRIVIELKRDANPNIVLNQLYKH
TKLQDTFGIIMLALVNNQPQILNLKEILVNYVEFQKEVIRRRTRFDLDKALARAHILEGLRIALDHIDEVIKLIRASKNT
AEAKEGLMNNFNLSEKQAQAILDMKLQRLTGLEREKIEEEYKELMEKISYFREILDKEELVLSIIKEELIEIKNKYGDER
KTEIVKGEHDIDIEDLIEDKKVIITLTHGGYIKRLDMDTYSSQKRGGKGIQATSTKQDDFIENMFVTSTHSTILFFTNRG
KVYKLKAYEIPEAGRTAKGTNIVNIIPIENNEKIQTVIGLKDIDDMKHFVMCTRNGIIKKTEIKKYSSIRKGGLNAINLR
EDDELIDVKMTKGNNEIIIVTQNGYCIRFNEEDVRPMGRVATGVKGITLREDDKAVSMEVVLEDEALLSISENGFGKRTD
IEEYPIHRRGGKGVITYKITDKTGPIVGARFIKEDDELMLVNSGDVAIRINVSEISKTSRNAMGVKLMRTSEEEKIVAIA
KIKSEDIIEEEILNEENLNEENLNEENLNEENLNEE

Sequences:

>Translated_836_residues
MLNEGKILPVDVSKEMKKCYIDYAMSVIAGRALPDVRDGLKPVHRRIIYSMQGLGLAPEKGYRKCARIVGDVLGKYHPHG
DTAVYEALVRMAQNFSIRYTLVDGHGNFGSVDGDGAAAMRYTEAKMSKISMELIKDINKNTVDFIPNFDGEEEEPSVLPS
RFPNLLVNGSSGIAVGMATNIPPHNLTEVIDGIIMLIENEDVNILDLMTKIKGPDFPTSGLIVGTRGIREAYETGRGKVI
IRAKAEIEEEKGKNKIIVTEIPYQVNKARLIENMANLVKDKKINGISDLRDESDRDGMRIVIELKRDANPNIVLNQLYKH
TKLQDTFGIIMLALVNNQPQILNLKEILVNYVEFQKEVIRRRTRFDLDKALARAHILEGLRIALDHIDEVIKLIRASKNT
AEAKEGLMNNFNLSEKQAQAILDMKLQRLTGLEREKIEEEYKELMEKISYFREILDKEELVLSIIKEELIEIKNKYGDER
KTEIVKGEHDIDIEDLIEDKKVIITLTHGGYIKRLDMDTYSSQKRGGKGIQATSTKQDDFIENMFVTSTHSTILFFTNRG
KVYKLKAYEIPEAGRTAKGTNIVNIIPIENNEKIQTVIGLKDIDDMKHFVMCTRNGIIKKTEIKKYSSIRKGGLNAINLR
EDDELIDVKMTKGNNEIIIVTQNGYCIRFNEEDVRPMGRVATGVKGITLREDDKAVSMEVVLEDEALLSISENGFGKRTD
IEEYPIHRRGGKGVITYKITDKTGPIVGARFIKEDDELMLVNSGDVAIRINVSEISKTSRNAMGVKLMRTSEEEKIVAIA
KIKSEDIIEEEILNEENLNEENLNEENLNEENLNEE
>Mature_836_residues
MLNEGKILPVDVSKEMKKCYIDYAMSVIAGRALPDVRDGLKPVHRRIIYSMQGLGLAPEKGYRKCARIVGDVLGKYHPHG
DTAVYEALVRMAQNFSIRYTLVDGHGNFGSVDGDGAAAMRYTEAKMSKISMELIKDINKNTVDFIPNFDGEEEEPSVLPS
RFPNLLVNGSSGIAVGMATNIPPHNLTEVIDGIIMLIENEDVNILDLMTKIKGPDFPTSGLIVGTRGIREAYETGRGKVI
IRAKAEIEEEKGKNKIIVTEIPYQVNKARLIENMANLVKDKKINGISDLRDESDRDGMRIVIELKRDANPNIVLNQLYKH
TKLQDTFGIIMLALVNNQPQILNLKEILVNYVEFQKEVIRRRTRFDLDKALARAHILEGLRIALDHIDEVIKLIRASKNT
AEAKEGLMNNFNLSEKQAQAILDMKLQRLTGLEREKIEEEYKELMEKISYFREILDKEELVLSIIKEELIEIKNKYGDER
KTEIVKGEHDIDIEDLIEDKKVIITLTHGGYIKRLDMDTYSSQKRGGKGIQATSTKQDDFIENMFVTSTHSTILFFTNRG
KVYKLKAYEIPEAGRTAKGTNIVNIIPIENNEKIQTVIGLKDIDDMKHFVMCTRNGIIKKTEIKKYSSIRKGGLNAINLR
EDDELIDVKMTKGNNEIIIVTQNGYCIRFNEEDVRPMGRVATGVKGITLREDDKAVSMEVVLEDEALLSISENGFGKRTD
IEEYPIHRRGGKGVITYKITDKTGPIVGARFIKEDDELMLVNSGDVAIRINVSEISKTSRNAMGVKLMRTSEEEKIVAIA
KIKSEDIIEEEILNEENLNEENLNEENLNEENLNEE

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=317, Percent_Identity=24.9211356466877, Blast_Score=84, Evalue=6e-16,
Organism=Homo sapiens, GI19913406, Length=177, Percent_Identity=28.2485875706215, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788562, Length=843, Percent_Identity=50.6524317912218, Blast_Score=862, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=673, Percent_Identity=35.9583952451709, Blast_Score=419, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17535065, Length=176, Percent_Identity=29.5454545454545, Blast_Score=74, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI212645845, Length=476, Percent_Identity=23.9495798319328, Blast_Score=73, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=378, Percent_Identity=24.8677248677249, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI17136538, Length=363, Percent_Identity=23.1404958677686, Blast_Score=74, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 94528; Mature: 94528

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNEGKILPVDVSKEMKKCYIDYAMSVIAGRALPDVRDGLKPVHRRIIYSMQGLGLAPEK
CCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
GYRKCARIVGDVLGKYHPHGDTAVYEALVRMAQNFSIRYTLVDGHGNFGSVDGDGAAAMR
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCHHEE
YTEAKMSKISMELIKDINKNTVDFIPNFDGEEEEPSVLPSRFPNLLVNGSSGIAVGMATN
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHCCCCEEEECCCCEEEEEECC
IPPHNLTEVIDGIIMLIENEDVNILDLMTKIKGPDFPTSGLIVGTRGIREAYETGRGKVI
CCCCHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHCCCCEEE
IRAKAEIEEEKGKNKIIVTEIPYQVNKARLIENMANLVKDKKINGISDLRDESDRDGMRI
EEEECHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEE
VIELKRDANPNIVLNQLYKHTKLQDTFGIIMLALVNNQPQILNLKEILVNYVEFQKEVIR
EEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCEECHHHHHHHHHHHHHHHHH
RRTRFDLDKALARAHILEGLRIALDHIDEVIKLIRASKNTAEAKEGLMNNFNLSEKQAQA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHH
ILDMKLQRLTGLEREKIEEEYKELMEKISYFREILDKEELVLSIIKEELIEIKNKYGDER
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCH
KTEIVKGEHDIDIEDLIEDKKVIITLTHGGYIKRLDMDTYSSQKRGGKGIQATSTKQDDF
HHHHCCCCCCCCHHHHHCCCEEEEEEECCCEEEEEECCCCCCHHCCCCCCEECCCCCHHH
IENMFVTSTHSTILFFTNRGKVYKLKAYEIPEAGRTAKGTNIVNIIPIENNEKIQTVIGL
HHHHEEEECCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEEECC
KDIDDMKHFVMCTRNGIIKKTEIKKYSSIRKGGLNAINLREDDELIDVKMTKGNNEIIIV
CCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCCEEEEE
TQNGYCIRFNEEDVRPMGRVATGVKGITLREDDKAVSMEVVLEDEALLSISENGFGKRTD
ECCCEEEEECCHHCCHHHHHHCCCCCEEEECCCCEEEEEEEEECCEEEEECCCCCCCCCC
IEEYPIHRRGGKGVITYKITDKTGPIVGARFIKEDDELMLVNSGDVAIRINVSEISKTSR
CHHCCCCCCCCCEEEEEEEECCCCCEEEHHHCCCCCCEEEEECCCEEEEEEHHHHHHCCC
NAMGVKLMRTSEEEKIVAIAKIKSEDIIEEEILNEENLNEENLNEENLNEENLNEE
CCCCEEEEECCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLNEGKILPVDVSKEMKKCYIDYAMSVIAGRALPDVRDGLKPVHRRIIYSMQGLGLAPEK
CCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
GYRKCARIVGDVLGKYHPHGDTAVYEALVRMAQNFSIRYTLVDGHGNFGSVDGDGAAAMR
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCHHEE
YTEAKMSKISMELIKDINKNTVDFIPNFDGEEEEPSVLPSRFPNLLVNGSSGIAVGMATN
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHCCCCEEEECCCCEEEEEECC
IPPHNLTEVIDGIIMLIENEDVNILDLMTKIKGPDFPTSGLIVGTRGIREAYETGRGKVI
CCCCHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCCCCCCEEEECHHHHHHHHCCCCEEE
IRAKAEIEEEKGKNKIIVTEIPYQVNKARLIENMANLVKDKKINGISDLRDESDRDGMRI
EEEECHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEE
VIELKRDANPNIVLNQLYKHTKLQDTFGIIMLALVNNQPQILNLKEILVNYVEFQKEVIR
EEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCEECHHHHHHHHHHHHHHHHH
RRTRFDLDKALARAHILEGLRIALDHIDEVIKLIRASKNTAEAKEGLMNNFNLSEKQAQA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHH
ILDMKLQRLTGLEREKIEEEYKELMEKISYFREILDKEELVLSIIKEELIEIKNKYGDER
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCH
KTEIVKGEHDIDIEDLIEDKKVIITLTHGGYIKRLDMDTYSSQKRGGKGIQATSTKQDDF
HHHHCCCCCCCCHHHHHCCCEEEEEEECCCEEEEEECCCCCCHHCCCCCCEECCCCCHHH
IENMFVTSTHSTILFFTNRGKVYKLKAYEIPEAGRTAKGTNIVNIIPIENNEKIQTVIGL
HHHHEEEECCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEEECC
KDIDDMKHFVMCTRNGIIKKTEIKKYSSIRKGGLNAINLREDDELIDVKMTKGNNEIIIV
CCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCCEEEEE
TQNGYCIRFNEEDVRPMGRVATGVKGITLREDDKAVSMEVVLEDEALLSISENGFGKRTD
ECCCEEEEECCHHCCHHHHHHCCCCCEEEECCCCEEEEEEEEECCEEEEECCCCCCCCCC
IEEYPIHRRGGKGVITYKITDKTGPIVGARFIKEDDELMLVNSGDVAIRINVSEISKTSR
CHHCCCCCCCCCEEEEEEEECCCCCEEEHHHCCCCCCEEEEECCCEEEEEEHHHHHHCCC
NAMGVKLMRTSEEEKIVAIAKIKSEDIIEEEILNEENLNEENLNEENLNEENLNEE
CCCCEEEEECCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]