| Definition | Clostridium botulinum A str. ATCC 3502, complete genome. |
|---|---|
| Accession | NC_009495 |
| Length | 3,886,916 |
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The map label for this gene is dnaG [H]
Identifier: 148378013
GI number: 148378013
Start: 1610
End: 2713
Strand: Direct
Name: dnaG [H]
Synonym: CBO0002
Alternate gene names: 148378013
Gene position: 1610-2713 (Clockwise)
Preceding gene: 148378012
Following gene: 148378014
Centisome position: 0.04
GC content: 25.36
Gene sequence:
>1104_bases ATGAAAATAACTTGTCAAAAAAACATTTTACTAGAAGGTATATCTATAGTTCAAAAAGCAGTAACTGGAAAATCAACTTT ACCTATATTATCTGGAATACTTATTAGAGCTAATAAAAATGAATTGGTCTTAACTGGTTCTGATATGGATCTTAGTATTG AAACTAAAGTAAAAGCTAACATTTTAGAAGAGGGAACTATAGTTTTAGATGCTAGACTTTTTGGTGAGATAATAAGAAAA TTACCTAATGATCTAATAGAAATAAATACGTTAGAAGATAATTCTATAGAAATAATATGTCAAAATTCTAGATTTAATCT AATATATATGAATCCAGGTGAGTTTCCTAATCCACCTATTATCAATGAAAATATGATATTTTCTATAGGTGAAAGTAAAT TAAAAAATATGATTAAAGGAACTATATTTGCTACAGCTCAAGATGAAACAAGACCTATTCTTACAGGTGTTTTATTTCAA ATAAAAGACAAAATGTTAAATTTAGTAGCTTTAGATGGCTATAGGTTAGCTTTAAGAAGCGAAGTTGTTGATAATGATAA TACGATAAATGCTGTTATTCCAGGGAAAACATTAAATGAAGTTTCAAAAATTTTAGAAGAAGATAATGAAAATGTTAATA TTACATTTACTCCTAATCATATTTTATTTAGTATAGGAGAAACAAAAATAATTTCAAGATTATTAGAAGGAGAATTTATT AGCTATAAGTCTATAATACCTGAAGAATTTAACTTAAAAATAATAGCTAAAAGATCAGAGCTTTTAAATTCTATAGAAAG AGCCTCTCTTATGGCTAAAGAAGGAAATACTAATTTAGTTAAATTTGATTTCTCAGATGATAAAATTGTAATAACATCAA ATTCTCAATTAGGAATGGTTCGAGAAGAATTAAAAGTTGTATTGCAAGGAGAAGATTTGCAAATTGCATTTAATTCAAAA TATCTTTTAGATGTGTTGAAAACTATGGAAGATAATGAGGTTGTATTAGAATTTTCAAGTAGTGTAAGTCCTTGTATTAT AAAAAATACAGAAATAAACAATTGCACTTACCTAGTTTTACCAGTAAGATTAAACAATAATTAA
Upstream 100 bases:
>100_bases TATTAACATATCAACAAGCCCTACTACTACTACTACTATTTTATTATATTATCTATTTCTATTCTAACAATAGGAATTGT TAATAAAGGAGGCTAATTTT
Downstream 100 bases:
>100_bases TTAAAAGAGGTATTAAAAATGGTTGAAATAAAAATTAATTCAGAAATAATAAAATTAGATTCTTTCTTAAAATGGTCAGG GGCAACAACTTTAGGTTCAG
Product: DNA polymerase III subunit beta
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 367; Mature: 367
Protein sequence:
>367_residues MKITCQKNILLEGISIVQKAVTGKSTLPILSGILIRANKNELVLTGSDMDLSIETKVKANILEEGTIVLDARLFGEIIRK LPNDLIEINTLEDNSIEIICQNSRFNLIYMNPGEFPNPPIINENMIFSIGESKLKNMIKGTIFATAQDETRPILTGVLFQ IKDKMLNLVALDGYRLALRSEVVDNDNTINAVIPGKTLNEVSKILEEDNENVNITFTPNHILFSIGETKIISRLLEGEFI SYKSIIPEEFNLKIIAKRSELLNSIERASLMAKEGNTNLVKFDFSDDKIVITSNSQLGMVREELKVVLQGEDLQIAFNSK YLLDVLKTMEDNEVVLEFSSSVSPCIIKNTEINNCTYLVLPVRLNNN
Sequences:
>Translated_367_residues MKITCQKNILLEGISIVQKAVTGKSTLPILSGILIRANKNELVLTGSDMDLSIETKVKANILEEGTIVLDARLFGEIIRK LPNDLIEINTLEDNSIEIICQNSRFNLIYMNPGEFPNPPIINENMIFSIGESKLKNMIKGTIFATAQDETRPILTGVLFQ IKDKMLNLVALDGYRLALRSEVVDNDNTINAVIPGKTLNEVSKILEEDNENVNITFTPNHILFSIGETKIISRLLEGEFI SYKSIIPEEFNLKIIAKRSELLNSIERASLMAKEGNTNLVKFDFSDDKIVITSNSQLGMVREELKVVLQGEDLQIAFNSK YLLDVLKTMEDNEVVLEFSSSVSPCIIKNTEINNCTYLVLPVRLNNN >Mature_367_residues MKITCQKNILLEGISIVQKAVTGKSTLPILSGILIRANKNELVLTGSDMDLSIETKVKANILEEGTIVLDARLFGEIIRK LPNDLIEINTLEDNSIEIICQNSRFNLIYMNPGEFPNPPIINENMIFSIGESKLKNMIKGTIFATAQDETRPILTGVLFQ IKDKMLNLVALDGYRLALRSEVVDNDNTINAVIPGKTLNEVSKILEEDNENVNITFTPNHILFSIGETKIISRLLEGEFI SYKSIIPEEFNLKIIAKRSELLNSIERASLMAKEGNTNLVKFDFSDDKIVITSNSQLGMVREELKVVLQGEDLQIAFNSK YLLDVLKTMEDNEVVLEFSSSVSPCIIKNTEINNCTYLVLPVRLNNN
Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped
COG id: COG0592
COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: NA
Homologues:
Organism=Escherichia coli, GI1790136, Length=367, Percent_Identity=28.6103542234332, Blast_Score=156, Evalue=2e-39,
Paralogues:
None
Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001001 - InterPro: IPR022635 - InterPro: IPR022637 - InterPro: IPR022634 [H]
Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]
EC number: =2.7.7.7 [H]
Molecular weight: Translated: 41093; Mature: 41093
Theoretical pI: Translated: 4.59; Mature: 4.59
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKITCQKNILLEGISIVQKAVTGKSTLPILSGILIRANKNELVLTGSDMDLSIETKVKAN CEEEECCCHHHHHHHHHHHHHCCCCCHHHHCCEEEEECCCEEEEECCCCCEEEEEEEEEE ILEEGTIVLDARLFGEIIRKLPNDLIEINTLEDNSIEIICQNSRFNLIYMNPGEFPNPPI EECCCEEEEEHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCE INENMIFSIGESKLKNMIKGTIFATAQDETRPILTGVLFQIKDKMLNLVALDGYRLALRS ECCCEEEEECHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECCEEEEEEH EVVDNDNTINAVIPGKTLNEVSKILEEDNENVNITFTPNHILFSIGETKIISRLLEGEFI HHCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCEEEEECCHHHHHHHHHCCCCE SYKSIIPEEFNLKIIAKRSELLNSIERASLMAKEGNTNLVKFDFSDDKIVITSNSQLGMV EHHHCCCCCCCEEEEEEHHHHHHHHHHHHHEEECCCCEEEEEECCCCEEEEECCCCCCHH REELKVVLQGEDLQIAFNSKYLLDVLKTMEDNEVVLEFSSSVSPCIIKNTEINNCTYLVL HHHEEEEEECCCEEEEECCHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCCEEEEEE PVRLNNN EEEECCC >Mature Secondary Structure MKITCQKNILLEGISIVQKAVTGKSTLPILSGILIRANKNELVLTGSDMDLSIETKVKAN CEEEECCCHHHHHHHHHHHHHCCCCCHHHHCCEEEEECCCEEEEECCCCCEEEEEEEEEE ILEEGTIVLDARLFGEIIRKLPNDLIEINTLEDNSIEIICQNSRFNLIYMNPGEFPNPPI EECCCEEEEEHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCE INENMIFSIGESKLKNMIKGTIFATAQDETRPILTGVLFQIKDKMLNLVALDGYRLALRS ECCCEEEEECHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECCEEEEEEH EVVDNDNTINAVIPGKTLNEVSKILEEDNENVNITFTPNHILFSIGETKIISRLLEGEFI HHCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCEEEEECCHHHHHHHHHCCCCE SYKSIIPEEFNLKIIAKRSELLNSIERASLMAKEGNTNLVKFDFSDDKIVITSNSQLGMV EHHHCCCCCCCEEEEEEHHHHHHHHHHHHHEEECCCCEEEEEECCCCEEEEECCCCCCHH REELKVVLQGEDLQIAFNSKYLLDVLKTMEDNEVVLEFSSSVSPCIIKNTEINNCTYLVL HHHEEEEEECCCEEEEECCHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCCEEEEEE PVRLNNN EEEECCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA