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The map label for this gene is thdF [H]

Identifier: 148361342

GI number: 148361342

Start: 3572650

End: 3573990

Strand: Reverse

Name: thdF [H]

Synonym: LPC_3317

Alternate gene names: 148361342

Gene position: 3573990-3572650 (Counterclockwise)

Preceding gene: 148361343

Following gene: 148361341

Centisome position: 99.93

GC content: 38.55

Gene sequence:

>1341_bases
ATGTCAATAGATACCATTGTTGCTATCGCTACCCCGCCGGGACGAGGCGGGGTAGGAATCGTCAGAATATCAGGTCCAAA
TGCTTATGCTATAGCCCTTTGCCTAAATGGGAACAAAGCATTGCAACCACGTTTAGCCACTTTTTGCTCTTTATATAAAG
GAAATAATGAAGTCCTTGATCAAGGATTGGTTCTTTATTTTAAAGGACCTCATTCTTTTACCGGCGAGGATGTTATTGAA
ATTCAAGCGCATGGCTCACCTGTGGTTTTGGATTTATTAATTAAGGAATCCATAGCAGCAGGGGCAAGGCTAGCCAGGCC
AGGTGAGTTTTCTGAAAGAGCATTTCTTAATGATAAAATCGATTTAATTCAAGCAGAAGCCATAGCTGATTTAATTCAAG
CCAGTTCAGATACCGCAGCTCGTATGGCTTTAAAATCACTGCAAGGTGATTTTTCTAAAAAAATAAATCAATTGAATGAA
GAGCTAATTTACTTACGCATGTATGTTGAAGCCGCTATCGATTTTCCTGAAGAGGAAATTGATTTTCTTAATGATGGAAA
TGTCAGTCAATTACTACAAAGAATTATTGGACGATTAGAAGAGATACGTTCACAAGCGAATCAAGGAGTTTTGTTGCGTG
AAGGGTTATCCTTGGTTATTGCTGGCAGGCCTAATGCAGGAAAATCAACCTTAATTAACAACCTGGCAGGGCGAGATGTT
GCCATTGTTACTGAAATTGCTGGTACTACGCGAGATATTATGCGAGAACATATCCTATTGGATGATATCCCTCTACACAT
TATCGATACGGCAGGATTAAGAGATAGCGATGATCTGGTGGAGAAAGAGGGAATAAAACGTGCTTGGCAAGAGTTGAAGC
GAGCTGATTGCGTGCTTCTTGTAGTAGATATTAACAATCCGGATCAACAAAATTCTCTGCTTAATGAATTGAGATTAACA
TTACCCAATAAAATACCCATAATCACGGTATACAATAAAATTGATACTACTAAACTTACAGCAAAATGTGATGAGCATAC
GGTTTATTTGTCTGCAAAAACTGGTGAGGGACTGGATGAGCTAAAAAAAGTGATCAAACAAGTGGTTGGATATCAACCAA
CAGAAGGCCAATTTTTAGCTCGCAGACGACATCTACAAGCGTTGGATGAAGCAAAAGCTTTGTTACTAACTGGGCAATCG
CAGCTAACAAATCATAAAGCTGGAGAATTGTTGGCAGAAGATTTACGGTTAGCTCATCAGACTTTATGTGAAATTACAGG
TGAATTCACTTCAGACGATTTATTAGGAAAAATTTTTTCCAGTTTTTGTATTGGAAAATAA

Upstream 100 bases:

>100_bases
GATTGTGAATAATACTTTATCAATATTACAGCAATGGTACATCACCAGAAAATACTCAGATGAAAAGCCAGCTAAAAAGG
TTGTTGCCACAGCAAAATAA

Downstream 100 bases:

>100_bases
CGATGATGCAAGGCTGTCATCATGTGAATTTTCAATTTTTCTTCTTTTGAATACAGTAAAATTATTATTAAATATTCTGT
GGTGATGTGCTTTATTGGGT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSIDTIVAIATPPGRGGVGIVRISGPNAYAIALCLNGNKALQPRLATFCSLYKGNNEVLDQGLVLYFKGPHSFTGEDVIE
IQAHGSPVVLDLLIKESIAAGARLARPGEFSERAFLNDKIDLIQAEAIADLIQASSDTAARMALKSLQGDFSKKINQLNE
ELIYLRMYVEAAIDFPEEEIDFLNDGNVSQLLQRIIGRLEEIRSQANQGVLLREGLSLVIAGRPNAGKSTLINNLAGRDV
AIVTEIAGTTRDIMREHILLDDIPLHIIDTAGLRDSDDLVEKEGIKRAWQELKRADCVLLVVDINNPDQQNSLLNELRLT
LPNKIPIITVYNKIDTTKLTAKCDEHTVYLSAKTGEGLDELKKVIKQVVGYQPTEGQFLARRRHLQALDEAKALLLTGQS
QLTNHKAGELLAEDLRLAHQTLCEITGEFTSDDLLGKIFSSFCIGK

Sequences:

>Translated_446_residues
MSIDTIVAIATPPGRGGVGIVRISGPNAYAIALCLNGNKALQPRLATFCSLYKGNNEVLDQGLVLYFKGPHSFTGEDVIE
IQAHGSPVVLDLLIKESIAAGARLARPGEFSERAFLNDKIDLIQAEAIADLIQASSDTAARMALKSLQGDFSKKINQLNE
ELIYLRMYVEAAIDFPEEEIDFLNDGNVSQLLQRIIGRLEEIRSQANQGVLLREGLSLVIAGRPNAGKSTLINNLAGRDV
AIVTEIAGTTRDIMREHILLDDIPLHIIDTAGLRDSDDLVEKEGIKRAWQELKRADCVLLVVDINNPDQQNSLLNELRLT
LPNKIPIITVYNKIDTTKLTAKCDEHTVYLSAKTGEGLDELKKVIKQVVGYQPTEGQFLARRRHLQALDEAKALLLTGQS
QLTNHKAGELLAEDLRLAHQTLCEITGEFTSDDLLGKIFSSFCIGK
>Mature_445_residues
SIDTIVAIATPPGRGGVGIVRISGPNAYAIALCLNGNKALQPRLATFCSLYKGNNEVLDQGLVLYFKGPHSFTGEDVIEI
QAHGSPVVLDLLIKESIAAGARLARPGEFSERAFLNDKIDLIQAEAIADLIQASSDTAARMALKSLQGDFSKKINQLNEE
LIYLRMYVEAAIDFPEEEIDFLNDGNVSQLLQRIIGRLEEIRSQANQGVLLREGLSLVIAGRPNAGKSTLINNLAGRDVA
IVTEIAGTTRDIMREHILLDDIPLHIIDTAGLRDSDDLVEKEGIKRAWQELKRADCVLLVVDINNPDQQNSLLNELRLTL
PNKIPIITVYNKIDTTKLTAKCDEHTVYLSAKTGEGLDELKKVIKQVVGYQPTEGQFLARRRHLQALDEAKALLLTGQSQ
LTNHKAGELLAEDLRLAHQTLCEITGEFTSDDLLGKIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=34.6808510638298, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI193082993, Length=461, Percent_Identity=34.9240780911063, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI193082988, Length=497, Percent_Identity=32.3943661971831, Blast_Score=209, Evalue=5e-54,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=59.8669623059867, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI87082120, Length=125, Percent_Identity=36.8, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17507259, Length=454, Percent_Identity=29.5154185022026, Blast_Score=178, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6323665, Length=500, Percent_Identity=31.8, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI45550813, Length=478, Percent_Identity=33.8912133891213, Blast_Score=204, Evalue=8e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 48920; Mature: 48789

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDTIVAIATPPGRGGVGIVRISGPNAYAIALCLNGNKALQPRLATFCSLYKGNNEVLD
CCCCEEEEEECCCCCCCEEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHC
QGLVLYFKGPHSFTGEDVIEIQAHGSPVVLDLLIKESIAAGARLARPGEFSERAFLNDKI
CCEEEEEECCCCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCH
DLIQAEAIADLIQASSDTAARMALKSLQGDFSKKINQLNEELIYLRMYVEAAIDFPEEEI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
DFLNDGNVSQLLQRIIGRLEEIRSQANQGVLLREGLSLVIAGRPNAGKSTLINNLAGRDV
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCCHHHHHHHCCCCCE
AIVTEIAGTTRDIMREHILLDDIPLHIIDTAGLRDSDDLVEKEGIKRAWQELKRADCVLL
EEEEHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
VVDINNPDQQNSLLNELRLTLPNKIPIITVYNKIDTTKLTAKCDEHTVYLSAKTGEGLDE
EEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCEEEEEEECCCCCHHH
LKKVIKQVVGYQPTEGQFLARRRHLQALDEAKALLLTGQSQLTNHKAGELLAEDLRLAHQ
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEECCHHHCCCHHHHHHHHHHHHHHH
TLCEITGEFTSDDLLGKIFSSFCIGK
HHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIDTIVAIATPPGRGGVGIVRISGPNAYAIALCLNGNKALQPRLATFCSLYKGNNEVLD
CCCEEEEEECCCCCCCEEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHC
QGLVLYFKGPHSFTGEDVIEIQAHGSPVVLDLLIKESIAAGARLARPGEFSERAFLNDKI
CCEEEEEECCCCCCCCCEEEEEECCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCH
DLIQAEAIADLIQASSDTAARMALKSLQGDFSKKINQLNEELIYLRMYVEAAIDFPEEEI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
DFLNDGNVSQLLQRIIGRLEEIRSQANQGVLLREGLSLVIAGRPNAGKSTLINNLAGRDV
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCCHHHHHHHCCCCCE
AIVTEIAGTTRDIMREHILLDDIPLHIIDTAGLRDSDDLVEKEGIKRAWQELKRADCVLL
EEEEHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
VVDINNPDQQNSLLNELRLTLPNKIPIITVYNKIDTTKLTAKCDEHTVYLSAKTGEGLDE
EEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCEEEEEEECCCCCHHH
LKKVIKQVVGYQPTEGQFLARRRHLQALDEAKALLLTGQSQLTNHKAGELLAEDLRLAHQ
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEECCHHHCCCHHHHHHHHHHHHHHH
TLCEITGEFTSDDLLGKIFSSFCIGK
HHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA