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The map label for this gene is rfbA [H]

Identifier: 148361199

GI number: 148361199

Start: 3435057

End: 3436550

Strand: Direct

Name: rfbA [H]

Synonym: LPC_3173

Alternate gene names: 148361199

Gene position: 3435057-3436550 (Clockwise)

Preceding gene: 148361195

Following gene: 148361200

Centisome position: 96.05

GC content: 42.24

Gene sequence:

>1494_bases
TTGCTCTTAAAAATTCCTGCTAAAATGCGGGTCAATTATGCCACAATATGGAATGTGATGACGACACCTCTATATCCAAT
AATTTTAGCCGGAGGCTCTGGCACTCGACTATGGCCTTTATCAAGACAAAATTTTCCTAAGCAATTTTTAAAATTAAATG
GCGAATTGTCTTTAATTCAACAAACCATGAAAAGAGCGATGGGATTGCCTGGCAATCAAACCATTATCGTAAGTAATGAT
GCTCATTACTTTATCTGTCAGGATCAATTGCAAGATTTTAATGCACAAACCACTTACCTGCTTGAGCCTTGTGCAAGAAA
CACAGCCCCTGCGATAGCATGCGCAGCCCATTATTTGGCAAACACAGTAGGAAAGGATGCTGTCATGCTGGTTTTACCCT
CGGATCACTGGATAGCCGATGATGAAATCTGGCTGGCCGCCATGTTAGAGGGAGGACAATTTGCCTCAGAAAACAATGCG
ATAGTGACATTTGGGATAAAGCCTGATAGCCCCAAAACCGGTTATGGCTACATTGAGGCCGGCACCACTTTATTCAGTGA
TATAAAGCAAGTTTTGAGCTTTAGAGAAAAACCAGACGCACACACTGCTGCACAATTCGTCAGTAAAGGGAATTATTTCT
GGAATAGCGGCATGTTCATTTGTCGTGCAGGGGTTTATCTTGAAGAGCTAGAACAATTTGAAGCCAACATCTACCAATTC
AGTCAACAGGCATTAACCCTTGCGCAGCATCATCATGATTTTTTGCGCCTTGATCTGGATTGTTTTTCTCAATGTAAAGA
AGAGTCTATAGACTATGCCATCATGGAAAAAACAGACAAGGCTGTTGTAATACCCATTTCAATACAATGGAGTGATCTGG
GCTGCTGGACCGCTGTGGCCGACGCGAATAAACAAGACGAACAAGGCAATACTCTCATTGGAAATGTCATCGCCCAGGAT
AGTCACAATTGCCTCATTAATAGTGAAGAGTTGTTAGTCACAACCGTTGGAATTCAGGATCAAATCATAGTTGCAACCAG
TGACGCTGTGTTAGTTGCCGATAAACGTTATTCGCAACAAGTGAAAGATTTGGTTCACTCCTTACGCAAAGATCATCCTC
ATTTAACTCAAGATCATCAACGTGTTCCCAGACCATGGGGGTATTATGAAGTGCTGGCAGAGGGCACCTCATTTAAAGTA
AAACGGCTTATGGTGAAGCCTGGTGCAAAACTCTCTTTACAAACGCATCAACATCGGGCTGAGCATTGGGTTATCGTTGG
CGGTGAGGCCGAGGTAGTTAATGGAAACAATACGTTCCGATTATCAAAAAACCAATCCACCTACATTCCGAAAAACGCAT
TGCATCGATTAAGTAACCCGCATAATGAACCACTTTATGTTATCGAAGTACAAAGTGGCTCCTATCTGGGCGAAGACGAC
ATCAAACGCTTTGATGATATTTATTTAAGAAAGGAAGTTGAACTGGCAGAATGA

Upstream 100 bases:

>100_bases
GACTTAGCCAACGCGAGACAAGTCGTTGAATCCAAACCGCCAGAAAGCAAGACCACAGCTTTTTTCATAATGCAAAACCT
CAAAAAATTGGAACAATTGC

Downstream 100 bases:

>100_bases
TGTCTATCGAAAATAACCCTCTTGATTCCACTCACTGTGCTTAGCCCTCTTGCCTAAGTTGCAATACACTTCATCCCTAT
TCCTGCAAATTTTAGTATCA

Product: phosphomannose isomerase GDP mannose pyrophosphorylase

Products: NA

Alternate protein names: Mannose-6-phosphate isomerase; Phosphohexomutase; Phosphomannose isomerase; PMI; Mannose-1-phosphate guanylyltransferase; GDP-mannose pyrophosphorylase; GMP; GMPP; GTP--mannose-1-phosphate guanylyltransferase [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MLLKIPAKMRVNYATIWNVMTTPLYPIILAGGSGTRLWPLSRQNFPKQFLKLNGELSLIQQTMKRAMGLPGNQTIIVSND
AHYFICQDQLQDFNAQTTYLLEPCARNTAPAIACAAHYLANTVGKDAVMLVLPSDHWIADDEIWLAAMLEGGQFASENNA
IVTFGIKPDSPKTGYGYIEAGTTLFSDIKQVLSFREKPDAHTAAQFVSKGNYFWNSGMFICRAGVYLEELEQFEANIYQF
SQQALTLAQHHHDFLRLDLDCFSQCKEESIDYAIMEKTDKAVVIPISIQWSDLGCWTAVADANKQDEQGNTLIGNVIAQD
SHNCLINSEELLVTTVGIQDQIIVATSDAVLVADKRYSQQVKDLVHSLRKDHPHLTQDHQRVPRPWGYYEVLAEGTSFKV
KRLMVKPGAKLSLQTHQHRAEHWVIVGGEAEVVNGNNTFRLSKNQSTYIPKNALHRLSNPHNEPLYVIEVQSGSYLGEDD
IKRFDDIYLRKEVELAE

Sequences:

>Translated_497_residues
MLLKIPAKMRVNYATIWNVMTTPLYPIILAGGSGTRLWPLSRQNFPKQFLKLNGELSLIQQTMKRAMGLPGNQTIIVSND
AHYFICQDQLQDFNAQTTYLLEPCARNTAPAIACAAHYLANTVGKDAVMLVLPSDHWIADDEIWLAAMLEGGQFASENNA
IVTFGIKPDSPKTGYGYIEAGTTLFSDIKQVLSFREKPDAHTAAQFVSKGNYFWNSGMFICRAGVYLEELEQFEANIYQF
SQQALTLAQHHHDFLRLDLDCFSQCKEESIDYAIMEKTDKAVVIPISIQWSDLGCWTAVADANKQDEQGNTLIGNVIAQD
SHNCLINSEELLVTTVGIQDQIIVATSDAVLVADKRYSQQVKDLVHSLRKDHPHLTQDHQRVPRPWGYYEVLAEGTSFKV
KRLMVKPGAKLSLQTHQHRAEHWVIVGGEAEVVNGNNTFRLSKNQSTYIPKNALHRLSNPHNEPLYVIEVQSGSYLGEDD
IKRFDDIYLRKEVELAE
>Mature_497_residues
MLLKIPAKMRVNYATIWNVMTTPLYPIILAGGSGTRLWPLSRQNFPKQFLKLNGELSLIQQTMKRAMGLPGNQTIIVSND
AHYFICQDQLQDFNAQTTYLLEPCARNTAPAIACAAHYLANTVGKDAVMLVLPSDHWIADDEIWLAAMLEGGQFASENNA
IVTFGIKPDSPKTGYGYIEAGTTLFSDIKQVLSFREKPDAHTAAQFVSKGNYFWNSGMFICRAGVYLEELEQFEANIYQF
SQQALTLAQHHHDFLRLDLDCFSQCKEESIDYAIMEKTDKAVVIPISIQWSDLGCWTAVADANKQDEQGNTLIGNVIAQD
SHNCLINSEELLVTTVGIQDQIIVATSDAVLVADKRYSQQVKDLVHSLRKDHPHLTQDHQRVPRPWGYYEVLAEGTSFKV
KRLMVKPGAKLSLQTHQHRAEHWVIVGGEAEVVNGNNTFRLSKNQSTYIPKNALHRLSNPHNEPLYVIEVQSGSYLGEDD
IKRFDDIYLRKEVELAE

Specific function: Produces a precursor for alginate polymerization. The alginate layer provides a protective barrier against host immune defenses and antibiotics [H]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=475, Percent_Identity=44.8421052631579, Blast_Score=407, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =5.3.1.8; =2.7.7.13 [H]

Molecular weight: Translated: 55944; Mature: 55944

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKIPAKMRVNYATIWNVMTTPLYPIILAGGSGTRLWPLSRQNFPKQFLKLNGELSLIQ
CEEECCHHHEEEHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCHHHHHCCCCHHHHH
QTMKRAMGLPGNQTIIVSNDAHYFICQDQLQDFNAQTTYLLEPCARNTAPAIACAAHYLA
HHHHHHHCCCCCCEEEEECCCEEEEEEHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHH
NTVGKDAVMLVLPSDHWIADDEIWLAAMLEGGQFASENNAIVTFGIKPDSPKTGYGYIEA
HHCCCCEEEEEECCCCEECCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCEEEEC
GTTLFSDIKQVLSFREKPDAHTAAQFVSKGNYFWNSGMFICRAGVYLEELEQFEANIYQF
CHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCEEEEECCHHHHHHHHHHHHHHHH
SQQALTLAQHHHDFLRLDLDCFSQCKEESIDYAIMEKTDKAVVIPISIQWSDLGCWTAVA
HHHHHHHHHHCCHHEEECHHHHHHHHHHCCCEEEEECCCCEEEEEEEEEECCCCCEEEEC
DANKQDEQGNTLIGNVIAQDSHNCLINSEELLVTTVGIQDQIIVATSDAVLVADKRYSQQ
CCCCCCCCCCEEEEEEEECCCCCEEECCCEEEEEEECCCCEEEEEECCEEEEECHHHHHH
VKDLVHSLRKDHPHLTQDHQRVPRPWGYYEVLAEGTSFKVKRLMVKPGAKLSLQTHQHRA
HHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHEECCCCEEEEEEEECCCCCEEEEHHCCCC
EHWVIVGGEAEVVNGNNTFRLSKNQSTYIPKNALHRLSNPHNEPLYVIEVQSGSYLGEDD
CEEEEECCCEEEECCCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCHHH
IKRFDDIYLRKEVELAE
HHHHHHHHHEEECCCCC
>Mature Secondary Structure
MLLKIPAKMRVNYATIWNVMTTPLYPIILAGGSGTRLWPLSRQNFPKQFLKLNGELSLIQ
CEEECCHHHEEEHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCHHHHHCCCCHHHHH
QTMKRAMGLPGNQTIIVSNDAHYFICQDQLQDFNAQTTYLLEPCARNTAPAIACAAHYLA
HHHHHHHCCCCCCEEEEECCCEEEEEEHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHH
NTVGKDAVMLVLPSDHWIADDEIWLAAMLEGGQFASENNAIVTFGIKPDSPKTGYGYIEA
HHCCCCEEEEEECCCCEECCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCEEEEC
GTTLFSDIKQVLSFREKPDAHTAAQFVSKGNYFWNSGMFICRAGVYLEELEQFEANIYQF
CHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCEEEEECCHHHHHHHHHHHHHHHH
SQQALTLAQHHHDFLRLDLDCFSQCKEESIDYAIMEKTDKAVVIPISIQWSDLGCWTAVA
HHHHHHHHHHCCHHEEECHHHHHHHHHHCCCEEEEECCCCEEEEEEEEEECCCCCEEEEC
DANKQDEQGNTLIGNVIAQDSHNCLINSEELLVTTVGIQDQIIVATSDAVLVADKRYSQQ
CCCCCCCCCCEEEEEEEECCCCCEEECCCEEEEEEECCCCEEEEEECCEEEEECHHHHHH
VKDLVHSLRKDHPHLTQDHQRVPRPWGYYEVLAEGTSFKVKRLMVKPGAKLSLQTHQHRA
HHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHEECCCCEEEEEEEECCCCCEEEEHHCCCC
EHWVIVGGEAEVVNGNNTFRLSKNQSTYIPKNALHRLSNPHNEPLYVIEVQSGSYLGEDD
CEEEEECCCEEEECCCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCHHH
IKRFDDIYLRKEVELAE
HHHHHHHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]