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The map label for this gene is truB

Identifier: 148361084

GI number: 148361084

Start: 3298442

End: 3299353

Strand: Reverse

Name: truB

Synonym: LPC_3055

Alternate gene names: 148361084

Gene position: 3299353-3298442 (Counterclockwise)

Preceding gene: 148361085

Following gene: 148361083

Centisome position: 92.25

GC content: 38.93

Gene sequence:

>912_bases
ATGACAACAATTGAATCTCAGTGTTCCATAGATGGAATCCTGCTATTAAATAAACCACAAGGAATGACCTCTAACGCTGC
TCTTCAGAAAGCCAAACATTTGTTTGGTGCTAAAAAAGCTGGTCACACAGGCAGCTTGGATCCTTTAGCAACCGGTATGT
TACCTCTATGCTTTGGAGAAGCGACTAAAATATGCCAGTATCTCCTTAATGCGGATAAAAGCTATGAAACAATTGGGAGG
CTAGGTAGTAAGACAAATACTGCTGATTGTACTGGCGAAGTGATATTTTGCATTGAAAATTATACTGTTAGCCATGAGGA
AATGATTGCAACCCTAGAAAAATATAAGGGTAAAATCAAGCAAATTCCATCCATGTTTTCTGCTTTAAAGCACAAGGGGA
CCCCTTTGTATCGACTTGCCAGAGAAGGTATAGAGATAGAACGTAAGGCTAGGGATATAGTAATCAGCCAATTGAAGTTG
GAACAATTTGATGGTGAATGTTTTACATTAACGGTATCATGCAGCAAAGGAACCTATATACGTAATTTAGTGGAAGATAT
TGGTGATACGTTGAAGGCAGGAGCTCATATGACAAAATTGCATAGGCTCTACACTGCAGGGTTTGAAAATAATCGAATGT
ACACTCTCGATGAATTACAGGATATGCCTTTATCACAAAGGCTTGCTTGCTTAATCCCTATTGATCAGGCCATTCAGCAT
TTAACACCAGTAATTCTTTCAGATTCTGAGGTAACAGCAATTCGCCAGGGCAAGGTGATTTCAAATAAAACTGGTGCTGT
TGAAGGAGAAGATTTGCGCCTTTATGGCGAGCAATCCCAATTTATTGGCATTGGCCAAGCTTTAATTCATGGTGATATTA
AAGCAAAAAGGTTAGTTTCTTTTGCGTTGTAA

Upstream 100 bases:

>100_bases
ACTGTGCCGCAACTTCATTTTATTTATGATGAATCGATTGAGTATGGACAGCGGTTAAGCCGATTAATTGATGAAGTAAA
TCCCCCTGATTCAAGTAGTG

Downstream 100 bases:

>100_bases
TGACAATTTTTGTAAGAATTGCTGCCTACTTTGTAATAAGTATGAAATTTTTTACACAAATTTATATCCACTACTTGTTT
CATTTGACATGCCTTGGTAG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTTIESQCSIDGILLLNKPQGMTSNAALQKAKHLFGAKKAGHTGSLDPLATGMLPLCFGEATKICQYLLNADKSYETIGR
LGSKTNTADCTGEVIFCIENYTVSHEEMIATLEKYKGKIKQIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLKL
EQFDGECFTLTVSCSKGTYIRNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDELQDMPLSQRLACLIPIDQAIQH
LTPVILSDSEVTAIRQGKVISNKTGAVEGEDLRLYGEQSQFIGIGQALIHGDIKAKRLVSFAL

Sequences:

>Translated_303_residues
MTTIESQCSIDGILLLNKPQGMTSNAALQKAKHLFGAKKAGHTGSLDPLATGMLPLCFGEATKICQYLLNADKSYETIGR
LGSKTNTADCTGEVIFCIENYTVSHEEMIATLEKYKGKIKQIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLKL
EQFDGECFTLTVSCSKGTYIRNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDELQDMPLSQRLACLIPIDQAIQH
LTPVILSDSEVTAIRQGKVISNKTGAVEGEDLRLYGEQSQFIGIGQALIHGDIKAKRLVSFAL
>Mature_302_residues
TTIESQCSIDGILLLNKPQGMTSNAALQKAKHLFGAKKAGHTGSLDPLATGMLPLCFGEATKICQYLLNADKSYETIGRL
GSKTNTADCTGEVIFCIENYTVSHEEMIATLEKYKGKIKQIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLKLE
QFDGECFTLTVSCSKGTYIRNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDELQDMPLSQRLACLIPIDQAIQHL
TPVILSDSEVTAIRQGKVISNKTGAVEGEDLRLYGEQSQFIGIGQALIHGDIKAKRLVSFAL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=272, Percent_Identity=30.8823529411765, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI215599015, Length=260, Percent_Identity=26.1538461538462, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI4503337, Length=260, Percent_Identity=26.1538461538462, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI2367200, Length=300, Percent_Identity=43, Blast_Score=250, Evalue=9e-68,
Organism=Saccharomyces cerevisiae, GI6324037, Length=191, Percent_Identity=29.8429319371728, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=257, Percent_Identity=25.6809338521401, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281364189, Length=264, Percent_Identity=24.6212121212121, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281364187, Length=264, Percent_Identity=24.6212121212121, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281364185, Length=264, Percent_Identity=24.6212121212121, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281364183, Length=264, Percent_Identity=24.6212121212121, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI62471759, Length=264, Percent_Identity=24.6212121212121, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17975520, Length=264, Percent_Identity=24.6212121212121, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_LEGPA (Q5X1C5)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_125123.1
- ProteinModelPortal:   Q5X1C5
- SMR:   Q5X1C5
- STRING:   Q5X1C5
- GeneID:   3116997
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp2818
- LegioList:   lpp2818
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   PRK05033
- BioCyc:   LPNE297246:LPP2818-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 33373; Mature: 33242

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: ACT_SITE 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIESQCSIDGILLLNKPQGMTSNAALQKAKHLFGAKKAGHTGSLDPLATGMLPLCFGE
CCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHH
ATKICQYLLNADKSYETIGRLGSKTNTADCTGEVIFCIENYTVSHEEMIATLEKYKGKIK
HHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
QIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLKLEQFDGECFTLTVSCSKGTYI
HHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHH
RNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDELQDMPLSQRLACLIPIDQAIQH
HHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEHHHHHCCCHHHHHEEEECHHHHHHH
LTPVILSDSEVTAIRQGKVISNKTGAVEGEDLRLYGEQSQFIGIGQALIHGDIKAKRLVS
HCCEEECCCCEEHHHCCCEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHH
FAL
HCC
>Mature Secondary Structure 
TTIESQCSIDGILLLNKPQGMTSNAALQKAKHLFGAKKAGHTGSLDPLATGMLPLCFGE
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHH
ATKICQYLLNADKSYETIGRLGSKTNTADCTGEVIFCIENYTVSHEEMIATLEKYKGKIK
HHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
QIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLKLEQFDGECFTLTVSCSKGTYI
HHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHH
RNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDELQDMPLSQRLACLIPIDQAIQH
HHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEHHHHHCCCHHHHHEEEECHHHHHHH
LTPVILSDSEVTAIRQGKVISNKTGAVEGEDLRLYGEQSQFIGIGQALIHGDIKAKRLVS
HCCEEECCCCEEHHHCCCEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHH
FAL
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA