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The map label for this gene is estA [H]

Identifier: 148361016

GI number: 148361016

Start: 463265

End: 463912

Strand: Direct

Name: estA [H]

Synonym: LPC_2976

Alternate gene names: 148361016

Gene position: 463265-463912 (Clockwise)

Preceding gene: 148361021

Following gene: 148361015

Centisome position: 12.95

GC content: 41.2

Gene sequence:

>648_bases
ATGAAGGAACCTTTAGAGAAAGCACAAGCTTGTGTTATATGGATGCATGGGTTGGGAGCTGATGCCTCTGATATGATGGG
TTTAGCTGATCAGCTAACAGTAGAAGATACCGCTTTAAGGCATGTATTTCTTGATGCTCCTCGAAGACCAGTGGCTCTGA
ATGGAGGAATGGTTATGCCTGCGTGGTATGACATTTATGGATTAGGCTTTGTCGATGAAGAAGATAAGTCTGGCATTGAG
CAATCTGAATTGTTAATACGCAAGGTGGTTGACGCTCAATATAATTGTGGCTTTAAACCTCATCAAATCTTTTTAGCTGG
CTTTTCTCAAGGGGGAGCTATGGCATTGCACACGGCTCTTCATATGACTGAGCGATTATGCGGCGTTATTGCATTGTCCG
CTTATTTGCCCTTGGCTAAGCATAATAAGCCGCAACTTGATAAGAATACTCCTATCTTTATGGGGGCAGGACAATTTGAT
CCTTTAGTTCTGCCAAAATGGACTCTACAGAGTAAGGATTGGCTCTTGGCTAATGGGTATAATGAAGTCTCTTTTCATCA
ATACCCAATGGAACATTCCATATGTTTTGAAGAAATTAAAGATCTTAGCCTTTGGCTTAATAAGCAGGTTCAAGGAGTTT
TACAATGA

Upstream 100 bases:

>100_bases
ATAAATTGTTCAATATATTTTTAAATGTTAAACTTGCGCTGCTCTATTGCATTGGCGATAATTTACGTTTTTGCGTGGAG
AATTTGTTTTGAGCGTTTAT

Downstream 100 bases:

>100_bases
CAATAGTAAAAAGGTCAAGAACAGTACCTTATTCTTGTGAGCAAATGTATGGACTGGTTAATGATGTTGAACATTATTCC
GAGTTTCTACCTTATTGTGC

Product: carboxylesterase/phospholipase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MKEPLEKAQACVIWMHGLGADASDMMGLADQLTVEDTALRHVFLDAPRRPVALNGGMVMPAWYDIYGLGFVDEEDKSGIE
QSELLIRKVVDAQYNCGFKPHQIFLAGFSQGGAMALHTALHMTERLCGVIALSAYLPLAKHNKPQLDKNTPIFMGAGQFD
PLVLPKWTLQSKDWLLANGYNEVSFHQYPMEHSICFEEIKDLSLWLNKQVQGVLQ

Sequences:

>Translated_215_residues
MKEPLEKAQACVIWMHGLGADASDMMGLADQLTVEDTALRHVFLDAPRRPVALNGGMVMPAWYDIYGLGFVDEEDKSGIE
QSELLIRKVVDAQYNCGFKPHQIFLAGFSQGGAMALHTALHMTERLCGVIALSAYLPLAKHNKPQLDKNTPIFMGAGQFD
PLVLPKWTLQSKDWLLANGYNEVSFHQYPMEHSICFEEIKDLSLWLNKQVQGVLQ
>Mature_215_residues
MKEPLEKAQACVIWMHGLGADASDMMGLADQLTVEDTALRHVFLDAPRRPVALNGGMVMPAWYDIYGLGFVDEEDKSGIE
QSELLIRKVVDAQYNCGFKPHQIFLAGFSQGGAMALHTALHMTERLCGVIALSAYLPLAKHNKPQLDKNTPIFMGAGQFD
PLVLPKWTLQSKDWLLANGYNEVSFHQYPMEHSICFEEIKDLSLWLNKQVQGVLQ

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=212, Percent_Identity=36.7924528301887, Blast_Score=118, Evalue=3e-27,
Organism=Homo sapiens, GI9966764, Length=215, Percent_Identity=34.4186046511628, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI20270341, Length=221, Percent_Identity=25.7918552036199, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17508057, Length=215, Percent_Identity=31.6279069767442, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323147, Length=217, Percent_Identity=33.6405529953917, Blast_Score=104, Evalue=9e-24,
Organism=Drosophila melanogaster, GI45553055, Length=214, Percent_Identity=34.5794392523364, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45553057, Length=214, Percent_Identity=34.5794392523364, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21357257, Length=214, Percent_Identity=34.5794392523364, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078179, Length=215, Percent_Identity=26.5116279069767, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24045; Mature: 24045

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00659 GLYCOSYL_HYDROL_F5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEPLEKAQACVIWMHGLGADASDMMGLADQLTVEDTALRHVFLDAPRRPVALNGGMVMP
CCCHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEECC
AWYDIYGLGFVDEEDKSGIEQSELLIRKVVDAQYNCGFKPHQIFLAGFSQGGAMALHTAL
CHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHH
HMTERLCGVIALSAYLPLAKHNKPQLDKNTPIFMGAGQFDPLVLPKWTLQSKDWLLANGY
HHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEECCCCCCEECCCCCCCCCCEEEECCC
NEVSFHQYPMEHSICFEEIKDLSLWLNKQVQGVLQ
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEPLEKAQACVIWMHGLGADASDMMGLADQLTVEDTALRHVFLDAPRRPVALNGGMVMP
CCCHHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEECC
AWYDIYGLGFVDEEDKSGIEQSELLIRKVVDAQYNCGFKPHQIFLAGFSQGGAMALHTAL
CHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHH
HMTERLCGVIALSAYLPLAKHNKPQLDKNTPIFMGAGQFDPLVLPKWTLQSKDWLLANGY
HHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEECCCCCCEECCCCCCCCCCEEEECCC
NEVSFHQYPMEHSICFEEIKDLSLWLNKQVQGVLQ
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA