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The map label for this gene is purA [H]

Identifier: 148360898

GI number: 148360898

Start: 578008

End: 579303

Strand: Direct

Name: purA [H]

Synonym: LPC_2858

Alternate gene names: 148360898

Gene position: 578008-579303 (Clockwise)

Preceding gene: 148360899

Following gene: 148360897

Centisome position: 16.16

GC content: 42.36

Gene sequence:

>1296_bases
ATGGGTAAGAATGTTGTTGTTTTGGGTACACAATGGGGAGATGAAGGCAAAGGCAAAATTGTTGATTTGCTGACACAAGA
TGCTCAAGTGGTTGTCCGTTATCAAGGGGGCCATAATGCAGGCCATACATTAAAAATCAATGGAGTAAAAACAGTACTTC
GTTTAATTCCATCCGGGATGCTTCGCCCTAACGTAACCTGCTATATTGCAAATGGTGTGGTCTTATCACCTCAGGCATTA
CTTAGTGAAATTAAGGAGCTTGAGGGTAATGGCGTTAATGTGAGGGAGCGATTACGCATTAGCTTGGCTTGTCCCCTGAT
CCTCCCTTACCACATTGCTTTGGATAAGGCTCGTGAGACACATATGGGCAAGTCTGCAATTGGAACAACAGGTCGTGGTA
TAGGTCCTGCTTATGAGGATAAAGTGGCTCGGCGCGCTTTAAGAGTAGGTGATTTATTTCATAGAGACCGATTTGCCAAT
AAATTAACAGAATTATTGGATTATCATAATTTTGTATTAACACAATATTTCAAACAACCAGCTGTTGATTTGGAATCCCT
ATTGGATGAGTCATTACAATGGGTTGAAGAATTAAGACCAATGGTTTGCGATGTTTCTGCCTGTTTGCATGAACATAGAA
AACAAGGTGAAAACATTTTATTCGAAGGGGCGCAGGGTGTTTATTTAGATATTGATCATGGAACATACCCCTACGTGACT
TCTTCAAATACCTGTGTGGGATCTGTAATTAATGGTGCGGGATTTGGTCCTCGCTATATTGATTACGTGTTAGGTATCAC
GAAAGCTTACACAACTCGTGTTGGTGGTGGCCCATTCCCCACTGAGTTACTGGATGACGTTGGAAAACGCATTGCGGAAC
GAGGACAGGAATTCGGCGCAGTAACTGGTAGGCCCAGACGTTGTGGGTGGTTTGACGCTGTTTTATTAAAACGCTCGATT
GAGTTAAATAGCATTTCTGGACTCTGCGTTACCAAATTGGATGTGCTTGATGGTTTAGAGGTATTACGGATTGCTGTGGC
TTATAAAGACAGGGATGGCAATATATTATCTCGACCTCCTCTGGCAGCTGATGATTTCAACGATTTGTTACCAGTCTATG
AGGAGCTTCCTGGTTGGCAGGAATCTACTGCTGATGTGACTTTGATGAGTGACTTACCCGCCAATGCACGGGCTTATCTA
AAGCGAATAGAAGAGATATTAGGAATTCCTATTGATATGCTATCAACAGGACCTGAAAGGGATTCAACAATTACTTTACG
AGGTCCTTTTCTTTGA

Upstream 100 bases:

>100_bases
TATTTATTAGCATCATCCATCTAATATAAATGCAGTTGGGTTTCTCGTAAAATACAACTGCATTTTTAATTTTTACAGGT
ATGCAAACAAAGAGTAAATC

Downstream 100 bases:

>100_bases
TACAAAGATAAAGTTTTGTAACCACTATGAGTGTCATGCAAGGATTTCTTGATATGACACTTTCCGCCTTTTAAGTATTA
TTGTCTGTGTTTTAGCAAGC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MGKNVVVLGTQWGDEGKGKIVDLLTQDAQVVVRYQGGHNAGHTLKINGVKTVLRLIPSGMLRPNVTCYIANGVVLSPQAL
LSEIKELEGNGVNVRERLRISLACPLILPYHIALDKARETHMGKSAIGTTGRGIGPAYEDKVARRALRVGDLFHRDRFAN
KLTELLDYHNFVLTQYFKQPAVDLESLLDESLQWVEELRPMVCDVSACLHEHRKQGENILFEGAQGVYLDIDHGTYPYVT
SSNTCVGSVINGAGFGPRYIDYVLGITKAYTTRVGGGPFPTELLDDVGKRIAERGQEFGAVTGRPRRCGWFDAVLLKRSI
ELNSISGLCVTKLDVLDGLEVLRIAVAYKDRDGNILSRPPLAADDFNDLLPVYEELPGWQESTADVTLMSDLPANARAYL
KRIEEILGIPIDMLSTGPERDSTITLRGPFL

Sequences:

>Translated_431_residues
MGKNVVVLGTQWGDEGKGKIVDLLTQDAQVVVRYQGGHNAGHTLKINGVKTVLRLIPSGMLRPNVTCYIANGVVLSPQAL
LSEIKELEGNGVNVRERLRISLACPLILPYHIALDKARETHMGKSAIGTTGRGIGPAYEDKVARRALRVGDLFHRDRFAN
KLTELLDYHNFVLTQYFKQPAVDLESLLDESLQWVEELRPMVCDVSACLHEHRKQGENILFEGAQGVYLDIDHGTYPYVT
SSNTCVGSVINGAGFGPRYIDYVLGITKAYTTRVGGGPFPTELLDDVGKRIAERGQEFGAVTGRPRRCGWFDAVLLKRSI
ELNSISGLCVTKLDVLDGLEVLRIAVAYKDRDGNILSRPPLAADDFNDLLPVYEELPGWQESTADVTLMSDLPANARAYL
KRIEEILGIPIDMLSTGPERDSTITLRGPFL
>Mature_430_residues
GKNVVVLGTQWGDEGKGKIVDLLTQDAQVVVRYQGGHNAGHTLKINGVKTVLRLIPSGMLRPNVTCYIANGVVLSPQALL
SEIKELEGNGVNVRERLRISLACPLILPYHIALDKARETHMGKSAIGTTGRGIGPAYEDKVARRALRVGDLFHRDRFANK
LTELLDYHNFVLTQYFKQPAVDLESLLDESLQWVEELRPMVCDVSACLHEHRKQGENILFEGAQGVYLDIDHGTYPYVTS
SNTCVGSVINGAGFGPRYIDYVLGITKAYTTRVGGGPFPTELLDDVGKRIAERGQEFGAVTGRPRRCGWFDAVLLKRSIE
LNSISGLCVTKLDVLDGLEVLRIAVAYKDRDGNILSRPPLAADDFNDLLPVYEELPGWQESTADVTLMSDLPANARAYLK
RIEEILGIPIDMLSTGPERDSTITLRGPFL

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=430, Percent_Identity=42.3255813953488, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI22748717, Length=429, Percent_Identity=40.0932400932401, Blast_Score=302, Evalue=4e-82,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=37.844611528822, Blast_Score=257, Evalue=1e-68,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=63.4883720930233, Blast_Score=566, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI25146555, Length=428, Percent_Identity=41.8224299065421, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI25146553, Length=428, Percent_Identity=41.8224299065421, Blast_Score=322, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6324109, Length=433, Percent_Identity=42.7251732101617, Blast_Score=337, Evalue=2e-93,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=41.9811320754717, Blast_Score=336, Evalue=2e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47468; Mature: 47337

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNVVVLGTQWGDEGKGKIVDLLTQDAQVVVRYQGGHNAGHTLKINGVKTVLRLIPSGM
CCCCEEEEEECCCCCCCCCEEHEECCCCEEEEEECCCCCCCCEEEECHHHHHHHHHCCCC
LRPNVTCYIANGVVLSPQALLSEIKELEGNGVNVRERLRISLACPLILPYHIALDKARET
CCCCEEEEEECCEEECHHHHHHHHHHHCCCCCCHHHHHEEEEECHHHHHHHHHHHHHHHH
HMGKSAIGTTGRGIGPAYEDKVARRALRVGDLFHRDRFANKLTELLDYHNFVLTQYFKQP
HCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AVDLESLLDESLQWVEELRPMVCDVSACLHEHRKQGENILFEGAQGVYLDIDHGTYPYVT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCEEE
SSNTCVGSVINGAGFGPRYIDYVLGITKAYTTRVGGGPFPTELLDDVGKRIAERGQEFGA
CCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
VTGRPRRCGWFDAVLLKRSIELNSISGLCVTKLDVLDGLEVLRIAVAYKDRDGNILSRPP
CCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCC
LAADDFNDLLPVYEELPGWQESTADVTLMSDLPANARAYLKRIEEILGIPIDMLSTGPER
CCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHCCCCCC
DSTITLRGPFL
CCEEEEECCCC
>Mature Secondary Structure 
GKNVVVLGTQWGDEGKGKIVDLLTQDAQVVVRYQGGHNAGHTLKINGVKTVLRLIPSGM
CCCEEEEEECCCCCCCCCEEHEECCCCEEEEEECCCCCCCCEEEECHHHHHHHHHCCCC
LRPNVTCYIANGVVLSPQALLSEIKELEGNGVNVRERLRISLACPLILPYHIALDKARET
CCCCEEEEEECCEEECHHHHHHHHHHHCCCCCCHHHHHEEEEECHHHHHHHHHHHHHHHH
HMGKSAIGTTGRGIGPAYEDKVARRALRVGDLFHRDRFANKLTELLDYHNFVLTQYFKQP
HCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AVDLESLLDESLQWVEELRPMVCDVSACLHEHRKQGENILFEGAQGVYLDIDHGTYPYVT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCEEE
SSNTCVGSVINGAGFGPRYIDYVLGITKAYTTRVGGGPFPTELLDDVGKRIAERGQEFGA
CCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
VTGRPRRCGWFDAVLLKRSIELNSISGLCVTKLDVLDGLEVLRIAVAYKDRDGNILSRPP
CCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCC
LAADDFNDLLPVYEELPGWQESTADVTLMSDLPANARAYLKRIEEILGIPIDMLSTGPER
CCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHCCCCCC
DSTITLRGPFL
CCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA