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The map label for this gene is traC [H]

Identifier: 148360838

GI number: 148360838

Start: 631778

End: 633964

Strand: Direct

Name: traC [H]

Synonym: LPC_2798

Alternate gene names: 148360838

Gene position: 631778-633964 (Clockwise)

Preceding gene: 148360839

Following gene: 148360833

Centisome position: 17.66

GC content: 43.94

Gene sequence:

>2187_bases
ATGACTAAATTGTCTCACCATCAGGTTGTGGCCAACCAAATCATTGAAAGCCTCAAACAAGGAACCGCACCCTGGGTAAA
ACCATGGGAGCCAAGCACGGGCGGCGGCCAAATCCCGTTTAATCCAGTCACAGGAAAGCGGTATCGCGGCATTAATGCCT
TGTATTTGATGTTAAATCAAAGCGGGGATAACCGCTGGCTCACCTACAAACAGGCGCAAAGCATTGACGCGCAGGTTAGA
AAAGGAGAAAAAGGCACGACTGTTCAATACTGGAAATTTACTGATGAAAAAATCAAAAACGATGATGCGGGAAACCCAGT
CCTCGATGAGCAGGGAAATCCTGTCAAAGTGCAGGTTAACCTTGAAAGACCCAAGGTGTTTTACGCAACGGTATTTCATG
CCTCACAAATTGATAATATGCCGGAACTTGTCCAAAAAGAGCAGGACTGGTCTTTAATTGAAAGAGCAGAAAACCTGCTA
CTTAATTCTGGTGCTGCCATTTTTCATTCGGAGGCTGATAGAGCATTTTACAGGGTATCAACCGACAGCATTCACCTCCC
TCCCAAAGAGCAATTTAAATCAGCCGCCCATTATTACGTCACAGCCCTTCATGCGCTTGGTCATTGGAGCGGGCATCCTT
CAAGGCTTGATCGAGACCTACGCCATCCCTTTGGCAGTGAAGCTTACGCCAAAGAAGAACTAAGGGCTGAAATCGCCAGT
ATGCTGTTGGGTTCGGAGCTTGGTATTGGACATGACCCCTCCCAACACACGGCTTACATCAAATCATGGATTCGCGTTTT
AGAAGGTGATCCGCTGGAAATATTCAGAGCGTCAGCGGATGCTGAAAAAATTGTCAATCACATTTGCTCATTGGAACAAG
CACAAGACATCCAGCATGAGCAATCTATTGTAAACAGGGATGAAAAAAATAATGAGGAAACTCTAATGGAATCCGAAAAG
AATATCCCTGAAAAAGTCTGGCTTAGCATCCCATTCAAACAAAAAGAGTTAGCCAAATCCGTGGTAGGGAAGTTGCCCGA
TGGAACACCTGGCATTGCCTGGGATAAAACACAGAAATGCTGGTATGCAAAACCAGGTGTTTCCATTGAAAAAATCAAAG
CCTGGCTTCCAGAAAATCAAAACTCACCAGAAGACAAAGCACTTTCACCAGCCGATGAATTCAAGGAAGCGTTAATCAGT
TTGGGTGCAGTATTGACAGGTGAGCATCCTATCATGGACGGCAAGACTCATCGTATACAAATGGAGGACGATAAAACTGG
TGAAAAGGCAGGATTTTACGTGGCCCATTTAGATGGTATTCCCGCAGGCTATATCAAAAATAACCGCACAGGTGCTGAGC
TAAAATGGAAAAGTAAAGGTTATGTTTTAACCGATGAACAAAAAGCAGCACTTAAGGCTCAGGTACTCGAAAATCAAAAA
AATCGCGAGCTTGAATTGGTGGAAAGGCACAAAAGCACCGCATTAAGGCTCAAGCAAAGGCTTTCCAAAATGAGCGAGAT
CACCGAGCCAACACCCTATATGAAATCAAAAGGCATTCAGGTTCATCCAGATGTTTATACTGATGATGACAAAAAATTAA
CGTGTATTCCTGCAACTGATATTGAAGGTACAATTTGGACGGTTCAATATATTGCAGAAGATGGCACCAAGCGCTTCGCT
AAAGATTCAAAAAAGGAAGGCTGCTTTCATGTTCTGGGCGGCATGAACAAACTTTTTGATGCGCCGGTTATTGTCATTGC
GGAAGGATATGCAACCGCTGCAACGATAAAAGAAGCATGCGGGCTGCCTGCTGTCGTTTCTGCCTTTGATTCAGGTAACC
TTAAATCGGTGGCGAAAGTATTGCATGAAAAATATCCATATACTCCCGTTATGATCGCATCTGACGATGATAAGCACCTG
GAGTTAACGCAAGGCATCAATCCGGGTAAAGAAAAAGCTTTGGAGGCTGCGGGTGCTGTTAATGGCTTTATTGTTATGCC
GACTTTCGCGCCCCATGAACAATCTTTAAACCCTAAAAAATTCAGCGATTTTAATGACTTGGCAAATCACAGTATGCTGG
GCTTGGATGGGGTTAAGCGTCAGCTCAAACCAAAAATTGAAAAAGTTGCCAAACGATGCAATTGTCTGCGCTTATTGCAA
AAAAGTAATGGGATTCATATTGCTTAG

Upstream 100 bases:

>100_bases
GTTCCGGGTTTTATCGCCGAAGTCACTCCCATCGAGGCAGAAATCATGGGTGCCTTTTCTGAGGATGCGCTTAGTGCTTA
TGACGCTAAGGAGGCGGCCT

Downstream 100 bases:

>100_bases
GTTATTTGATAAGGTGAAGTTTCTTACTTTCATCAATTAACCAGCGAAGGCCATCAGTAATCTTGGTTTGTAATAAAATA
TGGGCGCTTTCATTAAAGAA

Product: DNA primase TraC

Products: NA

Alternate protein names: Replication primase [H]

Number of amino acids: Translated: 728; Mature: 727

Protein sequence:

>728_residues
MTKLSHHQVVANQIIESLKQGTAPWVKPWEPSTGGGQIPFNPVTGKRYRGINALYLMLNQSGDNRWLTYKQAQSIDAQVR
KGEKGTTVQYWKFTDEKIKNDDAGNPVLDEQGNPVKVQVNLERPKVFYATVFHASQIDNMPELVQKEQDWSLIERAENLL
LNSGAAIFHSEADRAFYRVSTDSIHLPPKEQFKSAAHYYVTALHALGHWSGHPSRLDRDLRHPFGSEAYAKEELRAEIAS
MLLGSELGIGHDPSQHTAYIKSWIRVLEGDPLEIFRASADAEKIVNHICSLEQAQDIQHEQSIVNRDEKNNEETLMESEK
NIPEKVWLSIPFKQKELAKSVVGKLPDGTPGIAWDKTQKCWYAKPGVSIEKIKAWLPENQNSPEDKALSPADEFKEALIS
LGAVLTGEHPIMDGKTHRIQMEDDKTGEKAGFYVAHLDGIPAGYIKNNRTGAELKWKSKGYVLTDEQKAALKAQVLENQK
NRELELVERHKSTALRLKQRLSKMSEITEPTPYMKSKGIQVHPDVYTDDDKKLTCIPATDIEGTIWTVQYIAEDGTKRFA
KDSKKEGCFHVLGGMNKLFDAPVIVIAEGYATAATIKEACGLPAVVSAFDSGNLKSVAKVLHEKYPYTPVMIASDDDKHL
ELTQGINPGKEKALEAAGAVNGFIVMPTFAPHEQSLNPKKFSDFNDLANHSMLGLDGVKRQLKPKIEKVAKRCNCLRLLQ
KSNGIHIA

Sequences:

>Translated_728_residues
MTKLSHHQVVANQIIESLKQGTAPWVKPWEPSTGGGQIPFNPVTGKRYRGINALYLMLNQSGDNRWLTYKQAQSIDAQVR
KGEKGTTVQYWKFTDEKIKNDDAGNPVLDEQGNPVKVQVNLERPKVFYATVFHASQIDNMPELVQKEQDWSLIERAENLL
LNSGAAIFHSEADRAFYRVSTDSIHLPPKEQFKSAAHYYVTALHALGHWSGHPSRLDRDLRHPFGSEAYAKEELRAEIAS
MLLGSELGIGHDPSQHTAYIKSWIRVLEGDPLEIFRASADAEKIVNHICSLEQAQDIQHEQSIVNRDEKNNEETLMESEK
NIPEKVWLSIPFKQKELAKSVVGKLPDGTPGIAWDKTQKCWYAKPGVSIEKIKAWLPENQNSPEDKALSPADEFKEALIS
LGAVLTGEHPIMDGKTHRIQMEDDKTGEKAGFYVAHLDGIPAGYIKNNRTGAELKWKSKGYVLTDEQKAALKAQVLENQK
NRELELVERHKSTALRLKQRLSKMSEITEPTPYMKSKGIQVHPDVYTDDDKKLTCIPATDIEGTIWTVQYIAEDGTKRFA
KDSKKEGCFHVLGGMNKLFDAPVIVIAEGYATAATIKEACGLPAVVSAFDSGNLKSVAKVLHEKYPYTPVMIASDDDKHL
ELTQGINPGKEKALEAAGAVNGFIVMPTFAPHEQSLNPKKFSDFNDLANHSMLGLDGVKRQLKPKIEKVAKRCNCLRLLQ
KSNGIHIA
>Mature_727_residues
TKLSHHQVVANQIIESLKQGTAPWVKPWEPSTGGGQIPFNPVTGKRYRGINALYLMLNQSGDNRWLTYKQAQSIDAQVRK
GEKGTTVQYWKFTDEKIKNDDAGNPVLDEQGNPVKVQVNLERPKVFYATVFHASQIDNMPELVQKEQDWSLIERAENLLL
NSGAAIFHSEADRAFYRVSTDSIHLPPKEQFKSAAHYYVTALHALGHWSGHPSRLDRDLRHPFGSEAYAKEELRAEIASM
LLGSELGIGHDPSQHTAYIKSWIRVLEGDPLEIFRASADAEKIVNHICSLEQAQDIQHEQSIVNRDEKNNEETLMESEKN
IPEKVWLSIPFKQKELAKSVVGKLPDGTPGIAWDKTQKCWYAKPGVSIEKIKAWLPENQNSPEDKALSPADEFKEALISL
GAVLTGEHPIMDGKTHRIQMEDDKTGEKAGFYVAHLDGIPAGYIKNNRTGAELKWKSKGYVLTDEQKAALKAQVLENQKN
RELELVERHKSTALRLKQRLSKMSEITEPTPYMKSKGIQVHPDVYTDDDKKLTCIPATDIEGTIWTVQYIAEDGTKRFAK
DSKKEGCFHVLGGMNKLFDAPVIVIAEGYATAATIKEACGLPAVVSAFDSGNLKSVAKVLHEKYPYTPVMIASDDDKHLE
LTQGINPGKEKALEAAGAVNGFIVMPTFAPHEQSLNPKKFSDFNDLANHSMLGLDGVKRQLKPKIEKVAKRCNCLRLLQK
SNGIHIA

Specific function: Required for autonomous replication in E.coli. Transferred into the recipient cell during bacterial conjugation. Catalyzes the synthesis of short oligoribonucleotide primers with CpA or pCpA at their 5'-termini on a single stranded template DNA [H]

COG id: COG4227

COG function: function code L; Antirestriction protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013610
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08401 DUF1738; PF01751 Toprim [H]

EC number: NA

Molecular weight: Translated: 81342; Mature: 81211

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLSHHQVVANQIIESLKQGTAPWVKPWEPSTGGGQIPFNPVTGKRYRGINALYLMLNQ
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCEEEEEEEEC
SGDNRWLTYKQAQSIDAQVRKGEKGTTVQYWKFTDEKIKNDDAGNPVLDEQGNPVKVQVN
CCCCCEEEEHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCCCCCCCCCCEEEEEEE
LERPKVFYATVFHASQIDNMPELVQKEQDWSLIERAENLLLNSGAAIFHSEADRAFYRVS
ECCCEEEEEEEHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEC
TDSIHLPPKEQFKSAAHYYVTALHALGHWSGHPSRLDRDLRHPFGSEAYAKEELRAEIAS
CCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
MLLGSELGIGHDPSQHTAYIKSWIRVLEGDPLEIFRASADAEKIVNHICSLEQAQDIQHE
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QSIVNRDEKNNEETLMESEKNIPEKVWLSIPFKQKELAKSVVGKLPDGTPGIAWDKTQKC
HHHHCCCCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
WYAKPGVSIEKIKAWLPENQNSPEDKALSPADEFKEALISLGAVLTGEHPIMDGKTHRIQ
EECCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
MEDDKTGEKAGFYVAHLDGIPAGYIKNNRTGAELKWKSKGYVLTDEQKAALKAQVLENQK
ECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCEEEECCHHHHHHHHHHHCCC
NRELELVERHKSTALRLKQRLSKMSEITEPTPYMKSKGIQVHPDVYTDDDKKLTCIPATD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEECCCCEECCCCEEEEEECCC
IEGTIWTVQYIAEDGTKRFAKDSKKEGCFHVLGGMNKLFDAPVIVIAEGYATAATIKEAC
CCCCEEEEEEEHHCCHHHHHHCCCCCCHHHHHCCHHHHHCCCEEEEECCCHHHHHHHHHC
GLPAVVSAFDSGNLKSVAKVLHEKYPYTPVMIASDDDKHLELTQGINPGKEKALEAAGAV
CCCHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCEEHHCCCCCCHHHHHHHHCCC
NGFIVMPTFAPHEQSLNPKKFSDFNDLANHSMLGLDGVKRQLKPKIEKVAKRCNCLRLLQ
CCEEEECCCCCCCCCCCCCCCCCHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHH
KSNGIHIA
HCCCCCCC
>Mature Secondary Structure 
TKLSHHQVVANQIIESLKQGTAPWVKPWEPSTGGGQIPFNPVTGKRYRGINALYLMLNQ
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCEEEEEEEEC
SGDNRWLTYKQAQSIDAQVRKGEKGTTVQYWKFTDEKIKNDDAGNPVLDEQGNPVKVQVN
CCCCCEEEEHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCCCCCCCCCCEEEEEEE
LERPKVFYATVFHASQIDNMPELVQKEQDWSLIERAENLLLNSGAAIFHSEADRAFYRVS
ECCCEEEEEEEHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEC
TDSIHLPPKEQFKSAAHYYVTALHALGHWSGHPSRLDRDLRHPFGSEAYAKEELRAEIAS
CCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
MLLGSELGIGHDPSQHTAYIKSWIRVLEGDPLEIFRASADAEKIVNHICSLEQAQDIQHE
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QSIVNRDEKNNEETLMESEKNIPEKVWLSIPFKQKELAKSVVGKLPDGTPGIAWDKTQKC
HHHHCCCCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
WYAKPGVSIEKIKAWLPENQNSPEDKALSPADEFKEALISLGAVLTGEHPIMDGKTHRIQ
EECCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
MEDDKTGEKAGFYVAHLDGIPAGYIKNNRTGAELKWKSKGYVLTDEQKAALKAQVLENQK
ECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCEEEECCHHHHHHHHHHHCCC
NRELELVERHKSTALRLKQRLSKMSEITEPTPYMKSKGIQVHPDVYTDDDKKLTCIPATD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEECCCCEECCCCEEEEEECCC
IEGTIWTVQYIAEDGTKRFAKDSKKEGCFHVLGGMNKLFDAPVIVIAEGYATAATIKEAC
CCCCEEEEEEEHHCCHHHHHHCCCCCCHHHHHCCHHHHHCCCEEEEECCCHHHHHHHHHC
GLPAVVSAFDSGNLKSVAKVLHEKYPYTPVMIASDDDKHLELTQGINPGKEKALEAAGAV
CCCHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCEEHHCCCCCCHHHHHHHHCCC
NGFIVMPTFAPHEQSLNPKKFSDFNDLANHSMLGLDGVKRQLKPKIEKVAKRCNCLRLLQ
CCEEEECCCCCCCCCCCCCCCCCHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHH
KSNGIHIA
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1818755 [H]