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The map label for this gene is queF [H]

Identifier: 148360730

GI number: 148360730

Start: 746350

End: 747207

Strand: Direct

Name: queF [H]

Synonym: LPC_2678

Alternate gene names: 148360730

Gene position: 746350-747207 (Clockwise)

Preceding gene: 148360732

Following gene: 148360729

Centisome position: 20.87

GC content: 37.41

Gene sequence:

>858_bases
ATGAATAAGGAACTTGAAAGTATTTATCAGAATATAGCAAATCAATCGGAGCTGGGGCAAACTGCAAATTATGACAGCTA
CTATAATCCTAAAAGGCTGTATCCTATCCCCAGAGCCCCAAAACGCCAGGAAATTAATTTGGATCCCAACTCCACCACTT
TCTATGGTTTTGATTGCTGGAATCATTATGAGGTCTCCTGGTTAAACTCCAAAGGTAAACCTGTTGTTGCGATGGCTGTT
ATTTCTTATGATTGTCATTCCCCGTGTATTATTGAATCAAAGTCATTAAAACTCTATTTTAACTCGTTGAATAATAGTAC
TTTCCCTGATGTGGAAACCGTTGTGCAAACTATCAGCAAAGATTTAAGTTACTGCATTGGTTCTGAAGTGGCGGTTAATG
TGTACCCCTTGTCGGATATCCCTAATCAAACTATCTATGCTGCATTCGATGGTGTTTGCCTTGATAAGCTTGATATTGAA
TGCTCTGTATATCATGTAATGCCTGATTTTCTTTCGACCTCGTCTGAATTAGTTGAAGAGGTATTGTATTCTGATTTACT
TAAATCAAACTGCCTGGTTACCAACCAACCGGATTGGGGAAGCGTTCAAATTATTTATAAAGGTAAAAAGATAAATCATG
AAGGGTTGCTTAAATACCTGATTTCATTTAGAAATCATAATGAATTTCATGAGCAGTGCATTGAACGGATATTTGCAGAC
ATTATGCGATTTTGCCAACCTGAGTCATTGACTGTTTATGGACGATATACAAGAAGGGGAGGTCTGGATATTAACCCAAT
CAGGTCAACAGAACCTTGTGCTTTTGATGGGCAAAATATTCGTTTAATACGCCAATAA

Upstream 100 bases:

>100_bases
AAATATAGGCTATTTCCTTTTTTTTAAGAAAAAATTGACCCTTAGGTAACATGATAGATTATAATCACTTTGTTTTTTAG
AGTTACAAAAGGCATCCATA

Downstream 100 bases:

>100_bases
AAGAAGGTACTTATCCCCTAAGGGATAAATTTTAATCCCGCCTGGGAATTTAATAATTAATTTTCAATACATTACATCTT
TCCTATGCTAAGATTGCCTG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNKELESIYQNIANQSELGQTANYDSYYNPKRLYPIPRAPKRQEINLDPNSTTFYGFDCWNHYEVSWLNSKGKPVVAMAV
ISYDCHSPCIIESKSLKLYFNSLNNSTFPDVETVVQTISKDLSYCIGSEVAVNVYPLSDIPNQTIYAAFDGVCLDKLDIE
CSVYHVMPDFLSTSSELVEEVLYSDLLKSNCLVTNQPDWGSVQIIYKGKKINHEGLLKYLISFRNHNEFHEQCIERIFAD
IMRFCQPESLTVYGRYTRRGGLDINPIRSTEPCAFDGQNIRLIRQ

Sequences:

>Translated_285_residues
MNKELESIYQNIANQSELGQTANYDSYYNPKRLYPIPRAPKRQEINLDPNSTTFYGFDCWNHYEVSWLNSKGKPVVAMAV
ISYDCHSPCIIESKSLKLYFNSLNNSTFPDVETVVQTISKDLSYCIGSEVAVNVYPLSDIPNQTIYAAFDGVCLDKLDIE
CSVYHVMPDFLSTSSELVEEVLYSDLLKSNCLVTNQPDWGSVQIIYKGKKINHEGLLKYLISFRNHNEFHEQCIERIFAD
IMRFCQPESLTVYGRYTRRGGLDINPIRSTEPCAFDGQNIRLIRQ
>Mature_285_residues
MNKELESIYQNIANQSELGQTANYDSYYNPKRLYPIPRAPKRQEINLDPNSTTFYGFDCWNHYEVSWLNSKGKPVVAMAV
ISYDCHSPCIIESKSLKLYFNSLNNSTFPDVETVVQTISKDLSYCIGSEVAVNVYPLSDIPNQTIYAAFDGVCLDKLDIE
CSVYHVMPDFLSTSSELVEEVLYSDLLKSNCLVTNQPDWGSVQIIYKGKKINHEGLLKYLISFRNHNEFHEQCIERIFAD
IMRFCQPESLTVYGRYTRRGGLDINPIRSTEPCAFDGQNIRLIRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=271, Percent_Identity=48.7084870848708, Blast_Score=270, Evalue=9e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 32712; Mature: 32712

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKELESIYQNIANQSELGQTANYDSYYNPKRLYPIPRAPKRQEINLDPNSTTFYGFDCW
CCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCEECCCCCCCCCCEEECCCCCCEEEEEECC
NHYEVSWLNSKGKPVVAMAVISYDCHSPCIIESKSLKLYFNSLNNSTFPDVETVVQTISK
CCEEEEEECCCCCCEEEEEEEEECCCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHH
DLSYCIGSEVAVNVYPLSDIPNQTIYAAFDGVCLDKLDIECSVYHVMPDFLSTSSELVEE
HHHHHCCCCEEEEEEECCCCCCCEEEEEECCEEEECCCCEEEEEEECHHHHCCHHHHHHH
VLYSDLLKSNCLVTNQPDWGSVQIIYKGKKINHEGLLKYLISFRNHNEFHEQCIERIFAD
HHHHHHHHCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IMRFCQPESLTVYGRYTRRGGLDINPIRSTEPCAFDGQNIRLIRQ
HHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCEECCCCEEEEEC
>Mature Secondary Structure
MNKELESIYQNIANQSELGQTANYDSYYNPKRLYPIPRAPKRQEINLDPNSTTFYGFDCW
CCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCEECCCCCCCCCCEEECCCCCCEEEEEECC
NHYEVSWLNSKGKPVVAMAVISYDCHSPCIIESKSLKLYFNSLNNSTFPDVETVVQTISK
CCEEEEEECCCCCCEEEEEEEEECCCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHH
DLSYCIGSEVAVNVYPLSDIPNQTIYAAFDGVCLDKLDIECSVYHVMPDFLSTSSELVEE
HHHHHCCCCEEEEEEECCCCCCCEEEEEECCEEEECCCCEEEEEEECHHHHCCHHHHHHH
VLYSDLLKSNCLVTNQPDWGSVQIIYKGKKINHEGLLKYLISFRNHNEFHEQCIERIFAD
HHHHHHHHCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IMRFCQPESLTVYGRYTRRGGLDINPIRSTEPCAFDGQNIRLIRQ
HHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA