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The map label for this gene is fleQ [H]

Identifier: 148360501

GI number: 148360501

Start: 1034542

End: 1035957

Strand: Direct

Name: fleQ [H]

Synonym: LPC_2442

Alternate gene names: 148360501

Gene position: 1034542-1035957 (Clockwise)

Preceding gene: 148360502

Following gene: 148360498

Centisome position: 28.93

GC content: 39.34

Gene sequence:

>1416_bases
ATGAGGAGCAATGACAGGATTTATATTATCGATGATGATAAGGAACGTTGTGACAAGCTTCGGACTATTTTTGATTTTAT
AGGCCAATTCTCTGAGGTCACTCAATATGGCAATTGGCAATTGAGTGTTAATCCTAATCCAAGTGTGATAGTTCTTGGAG
CGTGCACTTCTTTTGAAAAAATGATGGATGAATTGGATACATTAGTTAAAAGGTTTCCAAAAACTCCAATATTGGTTATT
GATGCTGTGCTTTCTACATCTGAATGCGCGGATCGAAATATCGTTGATTGTTTATCATTTCCATTCAGCTATGCAAAAAT
GCTTAAATCGCTCCATAGGTGTCAAATAGCGAAAGAAGCAGTTAAATTTATCACTGCAGGTACCCATAAGACTCCTTTAT
TTAGAAGCTTGGTTGGGAATAGTGAAGGTATTCGTCAAGTACGCAAACTAATTGAGCAAGTAGCTGATACTGAGGCCAGT
GTGTTGATTTTGGGTGAGTCAGGTACGGGTAAGGAAGTCGTTGCTCGCAATATTCATTCCCTTTCATCCCGAGCAAATAA
ACCATTTGTCCCTATCAATTGTGGAGCAATCCCGGGGGAATTATTGGAGAGTGAATTATTTGGTCATGAAAAAGGTGCAT
TTACAGGAGCGATTACATCAAGGCAAGGACGTTTTGAGTTGGCCAATGGAGGAACTCTATTCCTGGATGAAATAGGAGAT
ATGCCATTGGCTATGCAGGTTAAGTTATTAAGAGTTTTACAAGAACGTTGTTTTGAAAGAGTAGGTTCAAATAAAAGCAT
CGAAGTCAATGTTCGTATCATTGCTGCTACACATAGAAATTTAGAAATTGCTATTCAAGAAGGGAAATTTAGAGAAGATT
TGTTTTACAGATTGAATGTATTTCCCATTGAAATGCCTCCACTACGTAATAGAAGTAGTGATTTATCACTGCTTTTTAAT
GAGCTGGTTTCTCGAATTGAAGGAGAGGGCAGACCTACCGTTCGATTAATGCCCGATGCTATGGATGCTTTATGCGAATA
CAGCTGGCCAGGTAACGTGAGGGAGTTAGCTAATTTGGTGGAGAGATTATCTATTCTTTACCCTAATGGTATTATTGGCA
AGAGTGATTTACCTCATCGGTTTCAAGGTGAATATAAACCATCTTGTTCAGAGGCATCGGGGGCGGAACGTGAAACTTTG
TTGCAAATAATAAAGCAGGAGTCAGTAGCCAATGCAGAGGGCATTGATTTAAAAGAGCACTTGGTTAAAACAGAACTTGC
CTTGATTAGCCAGGCCTTAGATGAGTCAGATTGGGTTGTTGCTCATGCAGCCAGTTATCTCAACATGAGACGGACAACTT
TAGTTGAAAAAATGCGCAAATATGGCTTAACTCGACCAGAAAGAATGGAACGATAG

Upstream 100 bases:

>100_bases
GATTTTATGACGGTTAAAATAAATTGTGACAATTATTTGACAGTTATTCATTATGTGTCCGATACTTTAAATTAAATGAC
TCAAACTTAAGGATAGGTTT

Downstream 100 bases:

>100_bases
ATAGATACGGCTCACCTCGTATCACTTGTTTGCTAGCACAGGATTATTTCTCTTTTTCCTGTGTTTCTTCTGTATTTGAT
TCAGGTTTGCTTTCTGTTTT

Product: transcriptional regulator FleQ

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MRSNDRIYIIDDDKERCDKLRTIFDFIGQFSEVTQYGNWQLSVNPNPSVIVLGACTSFEKMMDELDTLVKRFPKTPILVI
DAVLSTSECADRNIVDCLSFPFSYAKMLKSLHRCQIAKEAVKFITAGTHKTPLFRSLVGNSEGIRQVRKLIEQVADTEAS
VLILGESGTGKEVVARNIHSLSSRANKPFVPINCGAIPGELLESELFGHEKGAFTGAITSRQGRFELANGGTLFLDEIGD
MPLAMQVKLLRVLQERCFERVGSNKSIEVNVRIIAATHRNLEIAIQEGKFREDLFYRLNVFPIEMPPLRNRSSDLSLLFN
ELVSRIEGEGRPTVRLMPDAMDALCEYSWPGNVRELANLVERLSILYPNGIIGKSDLPHRFQGEYKPSCSEASGAERETL
LQIIKQESVANAEGIDLKEHLVKTELALISQALDESDWVVAHAASYLNMRRTTLVEKMRKYGLTRPERMER

Sequences:

>Translated_471_residues
MRSNDRIYIIDDDKERCDKLRTIFDFIGQFSEVTQYGNWQLSVNPNPSVIVLGACTSFEKMMDELDTLVKRFPKTPILVI
DAVLSTSECADRNIVDCLSFPFSYAKMLKSLHRCQIAKEAVKFITAGTHKTPLFRSLVGNSEGIRQVRKLIEQVADTEAS
VLILGESGTGKEVVARNIHSLSSRANKPFVPINCGAIPGELLESELFGHEKGAFTGAITSRQGRFELANGGTLFLDEIGD
MPLAMQVKLLRVLQERCFERVGSNKSIEVNVRIIAATHRNLEIAIQEGKFREDLFYRLNVFPIEMPPLRNRSSDLSLLFN
ELVSRIEGEGRPTVRLMPDAMDALCEYSWPGNVRELANLVERLSILYPNGIIGKSDLPHRFQGEYKPSCSEASGAERETL
LQIIKQESVANAEGIDLKEHLVKTELALISQALDESDWVVAHAASYLNMRRTTLVEKMRKYGLTRPERMER
>Mature_471_residues
MRSNDRIYIIDDDKERCDKLRTIFDFIGQFSEVTQYGNWQLSVNPNPSVIVLGACTSFEKMMDELDTLVKRFPKTPILVI
DAVLSTSECADRNIVDCLSFPFSYAKMLKSLHRCQIAKEAVKFITAGTHKTPLFRSLVGNSEGIRQVRKLIEQVADTEAS
VLILGESGTGKEVVARNIHSLSSRANKPFVPINCGAIPGELLESELFGHEKGAFTGAITSRQGRFELANGGTLFLDEIGD
MPLAMQVKLLRVLQERCFERVGSNKSIEVNVRIIAATHRNLEIAIQEGKFREDLFYRLNVFPIEMPPLRNRSSDLSLLFN
ELVSRIEGEGRPTVRLMPDAMDALCEYSWPGNVRELANLVERLSILYPNGIIGKSDLPHRFQGEYKPSCSEASGAERETL
LQIIKQESVANAEGIDLKEHLVKTELALISQALDESDWVVAHAASYLNMRRTTLVEKMRKYGLTRPERMER

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=472, Percent_Identity=37.5, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI87082117, Length=332, Percent_Identity=46.3855421686747, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI1790437, Length=382, Percent_Identity=42.1465968586387, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1789087, Length=380, Percent_Identity=41.8421052631579, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI1789233, Length=347, Percent_Identity=44.6685878962536, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1790299, Length=407, Percent_Identity=39.0663390663391, Blast_Score=258, Evalue=7e-70,
Organism=Escherichia coli, GI1788905, Length=240, Percent_Identity=53.3333333333333, Blast_Score=258, Evalue=8e-70,
Organism=Escherichia coli, GI87082152, Length=231, Percent_Identity=50.6493506493506, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1786524, Length=244, Percent_Identity=46.7213114754098, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI87081872, Length=247, Percent_Identity=43.7246963562753, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1787583, Length=230, Percent_Identity=43.4782608695652, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1789828, Length=232, Percent_Identity=37.9310344827586, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI87081858, Length=321, Percent_Identity=28.9719626168224, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52983; Mature: 52983

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSNDRIYIIDDDKERCDKLRTIFDFIGQFSEVTQYGNWQLSVNPNPSVIVLGACTSFEK
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCHHHHH
MMDELDTLVKRFPKTPILVIDAVLSTSECADRNIVDCLSFPFSYAKMLKSLHRCQIAKEA
HHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
VKFITAGTHKTPLFRSLVGNSEGIRQVRKLIEQVADTEASVLILGESGTGKEVVARNIHS
HHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHH
LSSRANKPFVPINCGAIPGELLESELFGHEKGAFTGAITSRQGRFELANGGTLFLDEIGD
HHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEECCCCEEEEECCCC
MPLAMQVKLLRVLQERCFERVGSNKSIEVNVRIIAATHRNLEIAIQEGKFREDLFYRLNV
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCEEEEEECCCCHHHHHEEEEE
FPIEMPPLRNRSSDLSLLFNELVSRIEGEGRPTVRLMPDAMDALCEYSWPGNVRELANLV
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCCCCCHHHHHHHH
ERLSILYPNGIIGKSDLPHRFQGEYKPSCSEASGAERETLLQIIKQESVANAEGIDLKEH
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
LVKTELALISQALDESDWVVAHAASYLNMRRTTLVEKMRKYGLTRPERMER
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC
>Mature Secondary Structure
MRSNDRIYIIDDDKERCDKLRTIFDFIGQFSEVTQYGNWQLSVNPNPSVIVLGACTSFEK
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCHHHHH
MMDELDTLVKRFPKTPILVIDAVLSTSECADRNIVDCLSFPFSYAKMLKSLHRCQIAKEA
HHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
VKFITAGTHKTPLFRSLVGNSEGIRQVRKLIEQVADTEASVLILGESGTGKEVVARNIHS
HHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHH
LSSRANKPFVPINCGAIPGELLESELFGHEKGAFTGAITSRQGRFELANGGTLFLDEIGD
HHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEECCCCEEEEECCCC
MPLAMQVKLLRVLQERCFERVGSNKSIEVNVRIIAATHRNLEIAIQEGKFREDLFYRLNV
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCEEEEEECCCCHHHHHEEEEE
FPIEMPPLRNRSSDLSLLFNELVSRIEGEGRPTVRLMPDAMDALCEYSWPGNVRELANLV
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCCCCCHHHHHHHH
ERLSILYPNGIIGKSDLPHRFQGEYKPSCSEASGAERETLLQIIKQESVANAEGIDLKEH
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
LVKTELALISQALDESDWVVAHAASYLNMRRTTLVEKMRKYGLTRPERMER
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225 [H]