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The map label for this gene is gdhA [H]

Identifier: 148360297

GI number: 148360297

Start: 1255293

End: 1256579

Strand: Direct

Name: gdhA [H]

Synonym: LPC_2234

Alternate gene names: 148360297

Gene position: 1255293-1256579 (Clockwise)

Preceding gene: 148360298

Following gene: 148360295

Centisome position: 35.1

GC content: 40.56

Gene sequence:

>1287_bases
ATGTACTTAGATGCTCTTTCACGGCTTGATAAGGCCGCTTCATTTTGTCGTATTGATTCCGAAGCTTTAGAGAAACTGAA
ACACCCTAAATCCTGTCTGGAAGTATCTCTCCCAGTTCGGATGGATAATGGTGAATTAAAAATTTTTACAGCTTATCGAG
TCCATCATAATGACAGCCGTGGTCCTATGAAAGGGGGCATACGGTTTCATCCAAAATTGGATTTAGATGAGATAAAAACT
CTTGCATTATGGATGACAATAAAATGCGCTGTCGTCGACATCCCCTTTGGTGGTGCAAAAGGTGGGGTAATTGTTGATCC
AAAACAATTATCCCGCATGGAGTTGGAGCGATTAAGTCGAAGTTACATTGAGTTAATAGCGGATTTTATTGGGCCAGACA
AAGACATCCCTGCTCCTGATATGTATACCAATGAGATGATCATGGGCTGGATGATGGATGAGTATGCCACCATTGTTCGG
CAAAATAGTCCAGCGGTAATCACAGGCAAGCCTATTTCTTTGGGAGGGTGCTTGGGGCGTGAGGGTGCGACCGGTCTAGG
TGCCTACTATTGCATTAAAATTCTTGAAAAAAAGAAAAAATGGCAGTCTTCTGAGCTACGAGTTGCTGTTCAAGGATTTG
GTAATGCGGGACAAAGTATTGCTAAGCTGCTTTACGACAATGGCTATAAAATTGTTGCAATCAGTGATTCGAAAGGAGGA
ATTTATAATACCAAAGGCATCGATATACCTCGCATGATTGAAATAAAAAACAGCTCTAAAGAGGTGCAGTCCATATATTG
CAAAGAGTCTGTTTGTAAACTTGCGAAGGATGCTACCATTACGAATGAAGAGTTATTGGAACTCGATGTCGATTTACTTA
TCCCAGCTGCTGCACAAAATCAAATTACCCAAGAAAATGCAGCCAGAATTAAGGCGCCAATAATCATTGAAATTGCAAAT
GGTCCAATAACCTTAGAGGCCGATGCACTATTGCAGAAAAAGGGAGTATTAATTGTACCCGACATATTAGCCAATACGGG
TGGGGTCATCGTAAGCTATTTTGAGTGGGTTCAAAATAAATCAGGTTATTATTGGAGTATGGAAAAAGTTCAAGAAGAAT
TACAGACTATCATATCCCGTGAATTTAAGAATATTTGGCAGCTGATGGAACAATATCAAACAGATATGCGGCAAGCGGCA
TACATTCATGCCTTGAGTCGATACGATAAAGCAGTTACAGCCCAAGGAACTCGCGGATATTTTTCAAATTTAAGCCTAAG
CCTATAA

Upstream 100 bases:

>100_bases
TTTGAACTTATTTTGAGTTCAGAGTAGGCCAAATAACAAATAGTTAGTACTCGTGGTTTGTTCAGAAGAAACCTCGTGAA
TGCACTAATTGGAGGAATGT

Downstream 100 bases:

>100_bases
GGAGAGTTATCCTGCTTGAATTTCATGCCTCTTCTTACTGGGGCTCATTGTCCTATTATTGGTGTTGATTGGGGTATTAG
GCTTACTCTCCCCAAAGAAT

Product: hypothetical protein

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MYLDALSRLDKAASFCRIDSEALEKLKHPKSCLEVSLPVRMDNGELKIFTAYRVHHNDSRGPMKGGIRFHPKLDLDEIKT
LALWMTIKCAVVDIPFGGAKGGVIVDPKQLSRMELERLSRSYIELIADFIGPDKDIPAPDMYTNEMIMGWMMDEYATIVR
QNSPAVITGKPISLGGCLGREGATGLGAYYCIKILEKKKKWQSSELRVAVQGFGNAGQSIAKLLYDNGYKIVAISDSKGG
IYNTKGIDIPRMIEIKNSSKEVQSIYCKESVCKLAKDATITNEELLELDVDLLIPAAAQNQITQENAARIKAPIIIEIAN
GPITLEADALLQKKGVLIVPDILANTGGVIVSYFEWVQNKSGYYWSMEKVQEELQTIISREFKNIWQLMEQYQTDMRQAA
YIHALSRYDKAVTAQGTRGYFSNLSLSL

Sequences:

>Translated_428_residues
MYLDALSRLDKAASFCRIDSEALEKLKHPKSCLEVSLPVRMDNGELKIFTAYRVHHNDSRGPMKGGIRFHPKLDLDEIKT
LALWMTIKCAVVDIPFGGAKGGVIVDPKQLSRMELERLSRSYIELIADFIGPDKDIPAPDMYTNEMIMGWMMDEYATIVR
QNSPAVITGKPISLGGCLGREGATGLGAYYCIKILEKKKKWQSSELRVAVQGFGNAGQSIAKLLYDNGYKIVAISDSKGG
IYNTKGIDIPRMIEIKNSSKEVQSIYCKESVCKLAKDATITNEELLELDVDLLIPAAAQNQITQENAARIKAPIIIEIAN
GPITLEADALLQKKGVLIVPDILANTGGVIVSYFEWVQNKSGYYWSMEKVQEELQTIISREFKNIWQLMEQYQTDMRQAA
YIHALSRYDKAVTAQGTRGYFSNLSLSL
>Mature_428_residues
MYLDALSRLDKAASFCRIDSEALEKLKHPKSCLEVSLPVRMDNGELKIFTAYRVHHNDSRGPMKGGIRFHPKLDLDEIKT
LALWMTIKCAVVDIPFGGAKGGVIVDPKQLSRMELERLSRSYIELIADFIGPDKDIPAPDMYTNEMIMGWMMDEYATIVR
QNSPAVITGKPISLGGCLGREGATGLGAYYCIKILEKKKKWQSSELRVAVQGFGNAGQSIAKLLYDNGYKIVAISDSKGG
IYNTKGIDIPRMIEIKNSSKEVQSIYCKESVCKLAKDATITNEELLELDVDLLIPAAAQNQITQENAARIKAPIIIEIAN
GPITLEADALLQKKGVLIVPDILANTGGVIVSYFEWVQNKSGYYWSMEKVQEELQTIISREFKNIWQLMEQYQTDMRQAA
YIHALSRYDKAVTAQGTRGYFSNLSLSL

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=350, Percent_Identity=40.8571428571429, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI4885281, Length=350, Percent_Identity=40.5714285714286, Blast_Score=267, Evalue=1e-71,
Organism=Escherichia coli, GI1788059, Length=401, Percent_Identity=31.9201995012469, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17544676, Length=370, Percent_Identity=38.1081081081081, Blast_Score=251, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6319256, Length=408, Percent_Identity=31.1274509803922, Blast_Score=155, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6324951, Length=391, Percent_Identity=29.4117647058824, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45553475, Length=376, Percent_Identity=37.2340425531915, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI45549226, Length=377, Percent_Identity=36.8700265251989, Blast_Score=249, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24649283, Length=342, Percent_Identity=39.766081871345, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24649460, Length=325, Percent_Identity=36.6153846153846, Blast_Score=202, Evalue=2e-52,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 47748; Mature: 47748

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLDALSRLDKAASFCRIDSEALEKLKHPKSCLEVSLPVRMDNGELKIFTAYRVHHNDSR
CCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHEEEEEEEECCCCEEEEEEEEEEECCCC
GPMKGGIRFHPKLDLDEIKTLALWMTIKCAVVDIPFGGAKGGVIVDPKQLSRMELERLSR
CCCCCCEEECCCCCHHHHHHHHHHEEEEEEEEEECCCCCCCCEEECHHHHHHHHHHHHHH
SYIELIADFIGPDKDIPAPDMYTNEMIMGWMMDEYATIVRQNSPAVITGKPISLGGCLGR
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCC
EGATGLGAYYCIKILEKKKKWQSSELRVAVQGFGNAGQSIAKLLYDNGYKIVAISDSKGG
CCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCC
IYNTKGIDIPRMIEIKNSSKEVQSIYCKESVCKLAKDATITNEELLELDVDLLIPAAAQN
EEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHEECCHHHH
QITQENAARIKAPIIIEIANGPITLEADALLQKKGVLIVPDILANTGGVIVSYFEWVQNK
HHHHHHHHHCCCCEEEEECCCCEEEEHHHHHHHCCCEEECHHHCCCCCHHHHHHHHHCCC
SGYYWSMEKVQEELQTIISREFKNIWQLMEQYQTDMRQAAYIHALSRYDKAVTAQGTRGY
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
FSNLSLSL
HCCCEECC
>Mature Secondary Structure
MYLDALSRLDKAASFCRIDSEALEKLKHPKSCLEVSLPVRMDNGELKIFTAYRVHHNDSR
CCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHEEEEEEEECCCCEEEEEEEEEEECCCC
GPMKGGIRFHPKLDLDEIKTLALWMTIKCAVVDIPFGGAKGGVIVDPKQLSRMELERLSR
CCCCCCEEECCCCCHHHHHHHHHHEEEEEEEEEECCCCCCCCEEECHHHHHHHHHHHHHH
SYIELIADFIGPDKDIPAPDMYTNEMIMGWMMDEYATIVRQNSPAVITGKPISLGGCLGR
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCEEECCCCCC
EGATGLGAYYCIKILEKKKKWQSSELRVAVQGFGNAGQSIAKLLYDNGYKIVAISDSKGG
CCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCC
IYNTKGIDIPRMIEIKNSSKEVQSIYCKESVCKLAKDATITNEELLELDVDLLIPAAAQN
EEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHEECCHHHH
QITQENAARIKAPIIIEIANGPITLEADALLQKKGVLIVPDILANTGGVIVSYFEWVQNK
HHHHHHHHHCCCCEEEEECCCCEEEEHHHHHHHCCCEEECHHHCCCCCHHHHHHHHHCCC
SGYYWSMEKVQEELQTIISREFKNIWQLMEQYQTDMRQAAYIHALSRYDKAVTAQGTRGY
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
FSNLSLSL
HCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7787182; 8590279; 8905231 [H]